ViewGene
Locus Name:
GeneID:
Location:

GeneID:6445
TrivialName: PA14_15520
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_15520
PAO1 Ortholog Locus:
Sequence Length:786
Protein Length:261
Start: 1313291
Stop: 1314076
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAK50311.1|U66917_80 (U66917) TrbJ protein [Pseudomonas sp. ADP]
Identities = 209/252 (82%), Positives = 224/252 (88%), Gaps = 1/252 (0%)
Sequence:
ATGAAGCACGTCCGTTTTTTAGCGGCTAAAACCGCGCTCGCCGCCGTTCTGGCCGGCAGCATCGTCATCACG
CCTGTGCAGGCGGGTATCCCCGTCATCGACGGCACCAACCTGTCGCAGAACATCATGACGGCCATCGAATCG
GTGGCTCAAACGCTCAAGCAAATTGAGCAGTACAGCACCCAGCTCCAGCAGTATCAGAACCAGCTCCAGAAC
ACGATGGCTCCGGCAGCCTATATCTGGGATCAGGCGCAGTCCACCATCAATGGGCTGATGAACGCCGTCAAC
ACGCTGGACTACTACAAGACGCAGCTCGGCAGCCTGGACAGCTACATCAGCAAGTTCCAGGACGTGAACTAC
TACAAGGGGTCACCGTGCTTCTCGGCTACTGGCTGCTCGGCCGCCGAATTGGCCGCCATGAACAACGTGCGG
CAGCTCGCCAGCCAGTCGCAGAAGAAGGCCAACGATGCGTTGTTCAAGGGCTTGGACAAGCAGCAATCGAAC
CTGACGGCCGACGCGGCGACGTTGCAGCGTCTCCAGTCGGCCGCGCAGGGTGCGACCGGCCAGATGCAGGCC
ATCGGCTACGCCAACCAGCTTGCCAGCCAGCAGGCCAACCAGCTCTTACAGATTCGTGGCCTGTTGATCGCG
CAGCAGAACGCGGTGGCGACCAAGATGCAGGCCGACGCCGACCTTGAGGCCCGGCAGCAGGCGGCGCACGCC
GCTTCGACGGAATCGCGGATCGGGAAAACGGCAAGCCCGAAAAACTGGCTCGAACTGACCCGCTGA
Translation:
MKHVRFLAAKTALAAVLAGSIVITPVQAGIPVIDGTNLSQNIMTAIESVAQTLKQIEQYSTQLQQYQNQLQN
TMAPAAYIWDQAQSTINGLMNAVNTLDYYKTQLGSLDSYISKFQDVNYYKGSPCFSATGCSAAELAAMNNVR
QLASQSQKKANDALFKGLDKQQSNLTADAATLQRLQSAAQGATGQMQAIGYANQLASQQANQLLQIRGLLIA
QQNAVATKMQADADLEARQQAAHAASTESRIGKTASPKNWLELTR*
AnnotationID:6446GeneID:6445
AnnotatorUID: danlee
Modification Date/Time: 2005-10-18 14:02:40
Gene Name:trbJ
Confidence Code:2
GeneProduct:mating pair formation protein TrbJ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(22) Related to phage, transposon, or plasmid
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG5314
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|55418017|ref|YP_133917.1|  TrbJ conjugal transfer/entry exclusion protein precursor [Plasmid pB3]
     Length=254
     Score =  402 bits (1032),  Expect = 8e-111
     Identities = 209/252 (82%), Positives = 224/252 (88%), Gaps = 1/252 (0%)

gi|77733992|emb|CAI10756.1|  TrbJ protein [IncP-1beta multiresistance plasmid pB8]
gi|72384312|ref|YP_293665.1|  TrbJ protein of DNA transfer system [Ralstonia eutropha JMP134]
gi|72123665|gb|AAZ65808.1|  TrbJ protein of DNA transfer system [Ralstonia eutropha JMP134]
gi|32455864|ref|NP_862517.1|  TrbJ protein [Pseudomonas sp. ADP]
gi|72607215|gb|EAO43180.1|  putative mating pair formation protein [Burkholderia ambifaria AMMD]
gi|13937500|gb|AAK50311.1|  TrbJ protein [Pseudomonas sp. ADP]
gi|10955219|ref|NP_044248.1|  TrbJ [Enterobacter aerogenes]
gi|7465747|pir||T08507  trbJ protein - Enterobacter aerogenes plasmid R751
gi|34500499|ref|NP_904270.1|  TrbJ [Delftia acidovorans]
     Length=254
     Score =  401 bits (1031),  Expect = 1e-110
     Identities = 209/252 (82%), Positives = 224/252 (88%), Gaps = 1/252 (0%)






 
Structural Features:
COG5314, Conjugal transfer/entry exclusion protein [Intracellular trafficking and secretion].
             CD-Length = 252 residues,  79.8% aligned
             Score = 87.8 bits (217), Expect = 1e-18






   
Genomic Context:






   
Comment:


















    ReferenceID:12699




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:16948




  
Author/Investigator(s):
        
Lessl,M., Krishnapillai,V. and Schilf,W.





  
Title:
         Identification and characterization of two entry exclusion genes of
            the promiscuous IncP plasmid R18




  
PubMed:
        1904533


MedLine:
        91260666




    
Source:

  AUTHORS   Lessl,M., Krishnapillai,V. and Schilf,W.
  JOURNAL   Mol. Gen. Genet. 227 (1), 120-126 (1991)






    
Reference Type:Journal 







    
Data:


    		




 
URL:

















    ReferenceID:29995




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6445
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
199221
                           gb|AAK38010.1|
                           (AF305549) mating pair formation protein TrbJ [Comamonas testosteroni]
                           261
                           80.84
                           86.59
                           7
                           1031.0
     

     
     
199222
                           gb|AAK50311.1|U66917_80
                           (U66917) TrbJ protein [Pseudomonas sp. ADP]
                           261
                           80.84
                           86.59
                           7
                           1031.0
     

     
     
199223
                           emb|CAD97496.1|
                           (AJ564903) TrbJ protein [uncultured bacterium]
                           261
                           80.84
                           86.59
                           7
                           1031.0
     

     
     
199224
                           gb|AAC64451.1|
                           (U67194) TrbJ [Enterobacter aerogenes]
                           261
                           80.84
                           86.59
                           7
                           1031.0
     

     
     
199225
                           dbj|BAC81993.1|
                           (AB063332) TrbJ [Delftia acidovorans]
                           261
                           80.84
                           86.59
                           7
                           1031.0
     

     






      

          
GC ORFID: 49447How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 3640646Stop: 3639861

          
Length: 786


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: Homolog Bit Score 

          
Other Homologs:  

          
GC ORF Sequence

           
ATGAAGCACGTCCGTTTTTTAGCGGCTAAAACCGCGCTCGCCGCCGTTCTGGCCGGCAGCATCGTCATCACG 
CCTGTGCAGGCGGGTATCCCCGTCATCGACGGCACCAACCTGTCGCAGAACATCATGACGGCCATCGAATCG 
GTGGCTCAAACGCTCAAGCAAATTGAGCAGTACAGCACCCAGCTCCAGCAGTATCAGAACCAGCTCCAGAAC 
ACGATGGCTCCGGCAGCCTATATCTGGGATCAGGCGCAGTCCACCATCAATGGGCTGATGAACGCCGTCAAC 
ACGCTGGACTACTACAAGACGCAGCTCGGCAGCCTGGACAGCTACATCAGCAAGTTCCAGGACGTGAACTAC 
TACAAGGGGTCACCGTGCTTCTCGGCTACTGGCTGCTCGGCCGCCGAATTGGCCGCCATGAACAACGTGCGG 
CAGCTCGCCAGCCAGTCGCAGAAGAAGGCCAACGATGCGTTGTTCAAGGGCTTGGACAAGCAGCAATCGAAC 
CTGACGGCCGACGCGGCGACGTTGCAGCGTCTCCAGTCGGCCGCGCAGGGTGCGACCGGCCAGATGCAGGCC 
ATCGGCTACGCCAACCAGCTTGCCAGCCAGCAGGCCAACCAGCTCTTACAGATTCGTGGCCTGTTGATCGCG 
CAGCAGAACGCGGTGGCGACCAAGATGCAGGCCGACGCCGACCTTGAGGCCCGGCAGCAGGCGGCGCACGCC 
GCTTCGACGGAATCGCGGATCGGGAAAACGGCAAGCCCGAAAAACTGGCTCGAACTGACCCGCTGA