ViewGene
Locus Name:
GeneID:
Location:

GeneID:644
TrivialName: PA14_41090
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41090
PAO1 Ortholog Locus:PA1812
Sequence Length:1605
Protein Length:534
Start: 3663439
Stop: 3665043
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05201.1|AE004607_7 (AE004607) membrane-bound lytic murein transglycosylase D precursor [Pseudomonas aeruginosa PAO1]
Identities = 534/534 (100%), Positives = 534/534 (100%)
Sequence:
ATGCCGCCACAGACACGTAAAACCCCCGATCTAGACGCCCTGGCTCGCGCCGTCCGGGTGTCCATCCTGCTC
ATCGCCGGTGCGCTCGCCGGCTGCCAGGGCAGCGGCCAGATCAAGGGGGAGGCCCAGGCCAAAGCAGCACGT
CCCGGCGCCCGAGCCGTCGACGTCCAGTTCAATCCCTCCTGGCTGCATACCCAGCCCGGCCAGAACGCCGCC
TACAACGACATCTGGGACCGCATGCGCGACGGTTTCCAGCTCCAGGATGCGATCAGCACCAACCCGCGCATC
GAACGCCAGCGCCTCTGGTTCCTCAGCAACCAGTCGTTCCTCGAACAATCGAGCGCGCGCGGCAGCCTCTAC
ATGCACTACGTGGTCGAGCGCCTGGAAGAACGCAACATGCCGCTCGAACTGGCGCTGCTGCCGGTCATCGAA
AGCGCCTACAACCCCTTCGCCCTGTCGCGCAGCAACGCCGCCGGCCTCTGGCAGTTCATTCCCGCCACCGGC
CAGCATTTCAACCTGCGCCAGACCAATTTCTACGACGGTCGCCGCGACATCACCGCCTCGACCAACGCCGCC
CTGACCTATCTCGAGCGCCTGCACGACATGTTCAACGGCGACTGGATGCTCGCCCTGGCGGCCTACAACGCC
GGCGAAGGCACTGTCAGCCGTGCCATCGAACGCAACGAGAAACTGGGCCTGCCGACCGACTACTGGAACCTG
CCGCTGCCGCAGGAAACCCAGGACTACGTACCCAAGCTGCTCGCCCTCTCGCAGATCGTCATGGCGCCGGAT
TCCTACGGCATCAGCCTGAACCCGATCAACAACGAGCCCTACTTCCAGGCCGTCCGGGTCAAGCGCGGGATC
GACCTGTCCAGCGTCGCCGCGCTGGCGAACCTGGACGAGGACGAGCTGTACCAGCTGAACCCGGCCTACAAG
CGCCGCGTGACCATGGACGGCCCCCAGCAACTGTTGGTGCCGATGGAGAAAGCGGCGTTCCTGACCGCCTCG
CTGGACACCCTCAAGCCGAAGGAAGTCACCGCCTGGCAGCAATACCGGGTGCGTAGCGGCGACAGCCTGCAC
AGCATCGCCAACCGCTATCGGATCACCGTAGCGGAGCTCAAGAGCGCCAACCGCCTGTCCAGCAACCACCTG
CGCAAGGGCCAGCAACTGAGCATCCCCGGGCAGATCGCCGGCGGCGCCGTCAAGCCGGTCTACCAGCAACTC
GCCCGACAGGCTTCCACCCCGGCCAGAACCCGCTCCTACAAGGTCAAGAACGGCGACAGCCTGTGGCAGATC
GCCCGCAACAACGGCGTCGACGTCAACGACCTGAAGCGCTGGAACGGCCTGGACAAGCACGCCCTGAAGGTC
GGCCAGACCCTCAAGCTGCAAGGCGGCACCCAGGCCCTGGCGGCCCGCAAGGGAAATGCCGCCGGCAAGCGC
GACAGCGCCACCTACTACAAGGTCAAGCAAGGCGACTCGATGTACCTGATCGCCAAGCGCTTCAATGTCGAG
ATGAAGCACCTGCAGCGCTGGAACCCGCGCAGCAAGCAGGCGCTCAAGCCCGGCCAGACCCTGACCCTGTAC
CTGGACACCGCGAGCCGCTGA
Translation:
MPPQTRKTPDLDALARAVRVSILLIAGALAGCQGSGQIKGEAQAKAARPGARAVDVQFNPSWLHTQPGQNAA
YNDIWDRMRDGFQLQDAISTNPRIERQRLWFLSNQSFLEQSSARGSLYMHYVVERLEERNMPLELALLPVIE
SAYNPFALSRSNAAGLWQFIPATGQHFNLRQTNFYDGRRDITASTNAALTYLERLHDMFNGDWMLALAAYNA
GEGTVSRAIERNEKLGLPTDYWNLPLPQETQDYVPKLLALSQIVMAPDSYGISLNPINNEPYFQAVRVKRGI
DLSSVAALANLDEDELYQLNPAYKRRVTMDGPQQLLVPMEKAAFLTASLDTLKPKEVTAWQQYRVRSGDSLH
SIANRYRITVAELKSANRLSSNHLRKGQQLSIPGQIAGGAVKPVYQQLARQASTPARTRSYKVKNGDSLWQI
ARNNGVDVNDLKRWNGLDKHALKVGQTLKLQGGTQALAARKGNAAGKRDSATYYKVKQGDSMYLIAKRFNVE
MKHLQRWNPRSKQALKPGQTLTLYLDTASR*
AnnotationID:653GeneID:644
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 13:01:59
Gene Name:mltD
Confidence Code:2
GeneProduct:membrane-bound lytic murein transglycosylase D precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam06474,pfam01464
Secondary Functional Category(ies):
EC Number:3.2.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57184.1| (AE016869) membrane-bound lytic murein transglycosylase D
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 530

 Score =  630 bits (1626), Expect = e-180
 Identities = 321/534 (60%), Positives = 399/534 (74%), Gaps = 11/534 (2%)

>gb|AAN69727.1|AE016609_1 (AE016789) membrane-bound lytic murein transglycosylase D
           [Pseudomonas putida KT2440]
          Length = 476

 Score =  574 bits (1479), Expect = e-163
 Identities = 299/532 (56%), Positives = 370/532 (69%), Gaps = 61/532 (11%)

>gb|AAF95381.1| (AE004295) membrane-bound lytic murein transglycosylase D [Vibrio
           cholerae]
          Length = 532

 Score =  336 bits (861), Expect = 2e-91
 Identities = 185/464 (39%), Positives = 282/464 (60%), Gaps = 18/464 (3%)






 
Structural Features:
pfam06474, MLTD_N, MLTD_N.
CD-Length = 93 residues, 100.0% aligned
Score = 98.1 bits (244), Expect = 2e-21
Identities = 37/95 (38%), Positives = 48/95 (50%), Gaps = 2/95 (2%)

pfam01464, SLT, Transglycosylase SLT domain.
CD-Length = 117 residues,  88.9% aligned
Score = 95.0 bits (236), Expect = 2e-20
Identities = 39/104 (37%), Positives = 53/104 (50%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1291




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18433




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37104




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:644_cdd.html


    		




 
URL:

















    ReferenceID:37797




  
Author/Investigator(s):
        Bateman A, Bycroft M.





  
Title:
        The structure of a LysM domain from E. coli membrane-bound lytic murein transglycosylase D (MltD).




  
PubMed:
        10843862


MedLine:
        




    
Source:
J Mol Biol. 2000 Jun 16;299(4):1113-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10843862









           
Homologs By Global Alignment 

           

       



     
Gene ID:644
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1092
                           PA1812_tr
                           translation of PA1812
                           534
                           99.99
                           99.99
                           0
                           2757.0
     

     
     
41513
                           gb|AAG05201.1|AE004607_7
                           (AE004607) membrane-bound lytic murein transglycosylase D precursor [Pseudomonas aeruginosa PAO1]
                           534
                           99.99
                           99.99
                           0
                           2757.0
     

     
     
41514
                           gb|AAO57184.1|
                           (AE016869) membrane-bound lytic murein transglycosylase D [Pseudomonas syringae pv. tomato str. DC3000]
                           542
                           59.59
                           73.80
                           20
                           1636.0
     

     
     
41515
                           gb|AAN69727.1|AE016609_1
                           (AE016789) membrane-bound lytic murein transglycosylase D [Pseudomonas putida KT2440]
                           537
                           55.67
                           68.90
                           64
                           1516.0
     

     
     
41516
                           dbj|BAC95297.1|
                           (AP005340) soluble lytic murein transglycosylase [Vibrio vulnificus YJ016]
                           580
                           36.20
                           54.13
                           79
                           886.0
     

     






      

          
GC ORFID: 43646How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1812Glimmer Score: 

          
Start: 1290470Stop: 1288866

          
Length: 1605


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1812 translation of PA1812Homolog Bit Score 1066.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene633 (ORF: BLASTX 633.0), t_PA1812 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGCCACAGACACGTAAAACCCCCGATCTAGACGCCCTGGCTCGCGCCGTCCGGGTGTCCATCCTGCTC 
ATCGCCGGTGCGCTCGCCGGCTGCCAGGGCAGCGGCCAGATCAAGGGGGAGGCCCAGGCCAAAGCAGCACGT 
CCCGGCGCCCGAGCCGTCGACGTCCAGTTCAATCCCTCCTGGCTGCATACCCAGCCCGGCCAGAACGCCGCC 
TACAACGACATCTGGGACCGCATGCGCGACGGTTTCCAGCTCCAGGATGCGATCAGCACCAACCCGCGCATC 
GAACGCCAGCGCCTCTGGTTCCTCAGCAACCAGTCGTTCCTCGAACAATCGAGCGCGCGCGGCAGCCTCTAC 
ATGCACTACGTGGTCGAGCGCCTGGAAGAACGCAACATGCCGCTCGAACTGGCGCTGCTGCCGGTCATCGAA 
AGCGCCTACAACCCCTTCGCCCTGTCGCGCAGCAACGCCGCCGGCCTCTGGCAGTTCATTCCCGCCACCGGC 
CAGCATTTCAACCTGCGCCAGACCAATTTCTACGACGGTCGCCGCGACATCACCGCCTCGACCAACGCCGCC 
CTGACCTATCTCGAGCGCCTGCACGACATGTTCAACGGCGACTGGATGCTCGCCCTGGCGGCCTACAACGCC 
GGCGAAGGCACTGTCAGCCGTGCCATCGAACGCAACGAGAAACTGGGCCTGCCGACCGACTACTGGAACCTG 
CCGCTGCCGCAGGAAACCCAGGACTACGTACCCAAGCTGCTCGCCCTCTCGCAGATCGTCATGGCGCCGGAT 
TCCTACGGCATCAGCCTGAACCCGATCAACAACGAGCCCTACTTCCAGGCCGTCCGGGTCAAGCGCGGGATC 
GACCTGTCCAGCGTCGCCGCGCTGGCGAACCTGGACGAGGACGAGCTGTACCAGCTGAACCCGGCCTACAAG 
CGCCGCGTGACCATGGACGGCCCCCAGCAACTGTTGGTGCCGATGGAGAAAGCGGCGTTCCTGACCGCCTCG 
CTGGACACCCTCAAGCCGAAGGAAGTCACCGCCTGGCAGCAATACCGGGTGCGTAGCGGCGACAGCCTGCAC 
AGCATCGCCAACCGCTATCGGATCACCGTAGCGGAGCTCAAGAGCGCCAACCGCCTGTCCAGCAACCACCTG 
CGCAAGGGCCAGCAACTGAGCATCCCCGGGCAGATCGCCGGCGGCGCCGTCAAGCCGGTCTACCAGCAACTC 
GCCCGACAGGCTTCCACCCCGGCCAGAACCCGCTCCTACAAGGTCAAGAACGGCGACAGCCTGTGGCAGATC 
GCCCGCAACAACGGCGTCGACGTCAACGACCTGAAGCGCTGGAACGGCCTGGACAAGCACGCCCTGAAGGTC 
GGCCAGACCCTCAAGCTGCAAGGCGGCACCCAGGCCCTGGCGGCCCGCAAGGGAAATGCCGCCGGCAAGCGC 
GACAGCGCCACCTACTACAAGGTCAAGCAAGGCGACTCGATGTACCTGATCGCCAAGCGCTTCAATGTCGAG 
ATGAAGCACCTGCAGCGCTGGAACCCGCGCAGCAAGCAGGCGCTCAAGCCCGGCCAGACCCTGACCCTGTAC 
CTGGACACCGCGAGCCGCTGA