ViewGene
Locus Name:
GeneID:
Location:

GeneID:639
TrivialName: PA14_13800
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_13800
PAO1 Ortholog Locus:PA3874
Sequence Length:1542
Protein Length:513
Start: 1187404
Stop: 1188945
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07261.1|AE004804_9 (AE004804) respiratory nitrate reductase beta chain [Pseudomonas aeruginosa PAO1]
Identities = 513/513 (100%), Positives = 513/513 (100%)
Sequence:
ATGAAAATTCGTTCGCAAGTCGGCATGGTGCTGAACCTCGACAAGTGCATTGGTTGCCACACCTGCTCGATC
ACCTGCAAGAACGTCTGGACCAGCCGCGAAGGCATGGAGTACGCCTGGTTCAACAACGTCGAGACCAAGCCC
GGGATCGGCTACCCGAAGGAATGGGAAAACCAGGAGAAGTGGAAGGGCGGCTGGGTGCGCGCGGCGGACGGT
TCGATCCGCCCGCGCATCGGCGGCAAGTTCCGCGTGCTGGCGAACATCTTCGCCAACCCGGACCTGCCCGAG
ATCGACGACTACTACGAACCGTTCGACTTCGATTACCAGCACCTGCATACCGCGCCCAAGGCCGAGCACCAG
CCGGTGGCGCGCCCGCGCTCGCTGGTCTCCGGGCAGCGCATGGAGAAGATCGAGTGGGGCCCGAACTGGGAG
GAGATCCTCGGCACCGAGTTCGCCAAGCGGCGCAAGGACAAGAACTTCGACCAGGTCCAGGCGGACATCTAC
GGTGAGTACGAGAACACCTTCATGATGTACCTGCCGCGCCTCTGCGAGCACTGCCTGAACCCGGCGTGCGTG
GCGTCCTGCCCGAGCGGGGCGATCTACAAGCGCGAGGAGGACGGCATCGTCCTGATCGACCAGGACAAGTGC
CGCGGCTGGCGGATGTGCATCTCCGGCTGCCCGTACAAGAAGATCTACTTCAACTGGAAGAGCGGCAAGTCC
GAGAAGTGCATCTTCTGCTACCCGCGCATCGAGGCCGGCCAGCCCACTGTCTGCTCGGAGACCTGCGTCGGG
CGCATCCGCTACCTCGGCGTGCTGCTCTACGACGCCGACCGCATCCACGAAGTGGCCAGTTGCGAGAACGAG
CGCGAGCTGTACGAGAAGCAACTGGAGATCTTCCTCGATCCGTTCGACCCGGCGGTGATCGCCCAGGCGCGC
AAGGACGGGGTGGCCGACAGCGTCATCGAGGCGGCGCAGAAGTCGCCGGTGTACAAGCTGGCGATGGACTGG
AAGCTGGCCCTGCCGCTGCACCCGGAATACCGCACGCTGCCGATGGTCTGGTACGTGCCACCGCTGTCGCCG
ATCCAGAACGCCGCCGCCGAGGGGCACATCGGCAGCGACGGGGTGATCCCGGACGTGGAGTCGCTGCGCATC
CCCGTGCAGTACCTGGCCAACCTGCTCACCGCCGGCGACACCGCGCCGGTGCTGCTGGCGCTCAAGCGCCTG
CTGGCGATGCGCGCCTACAAGCGCGCCGAGCACGTCGAAGGCCGCCAGGACCTGGAGGTGCTGGCCAAGGTC
GGGTTGAGCGTGGAGCAGGTGGAGGAGATGTACCGCTACCTGGCCATCGCCAACTACGAGGATCGCTTCGTG
ATCCCCAGCGCGCACCGCGAGGAAGCGCTTTCCGATGCCTTCGCCGAGCGTTCCGGCTGCGGCTTCAGCTTC
GGCAACGGCTGTTCCGGCGGCAGCAACTCCGCCGTCAACCTGTTCGGCGGCAAGCCGACCAACCGCCGCGAC
GTGATCCAGGTCGTGCAGATCCAGGAGTGA
Translation:
MKIRSQVGMVLNLDKCIGCHTCSITCKNVWTSREGMEYAWFNNVETKPGIGYPKEWENQEKWKGGWVRAADG
SIRPRIGGKFRVLANIFANPDLPEIDDYYEPFDFDYQHLHTAPKAEHQPVARPRSLVSGQRMEKIEWGPNWE
EILGTEFAKRRKDKNFDQVQADIYGEYENTFMMYLPRLCEHCLNPACVASCPSGAIYKREEDGIVLIDQDKC
RGWRMCISGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIHEVASCENE
RELYEKQLEIFLDPFDPAVIAQARKDGVADSVIEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSP
IQNAAAEGHIGSDGVIPDVESLRIPVQYLANLLTAGDTAPVLLALKRLLAMRAYKRAEHVEGRQDLEVLAKV
GLSVEQVEEMYRYLAIANYEDRFVIPSAHREEALSDAFAERSGCGFSFGNGCSGGSNSAVNLFGGKPTNRRD
VIQVVQIQE*
AnnotationID:648GeneID:639
AnnotatorUID: diggins
Modification Date/Time: 2005-04-25 09:09:22
Gene Name:narH
Confidence Code:2
GeneProduct:respiratory nitrate reductase beta subuni
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1140
Secondary Functional Category(ies):
EC Number:1.7.99.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG34374.1|AF197465_6 (AF197465) respiratory nitrate reductase beta subunit; NarH
           [Pseudomonas fluorescens]
          Length = 512

 Score =  934 bits (2414), Expect = 0.0
 Identities = 439/513 (85%), Positives = 474/513 (92%), Gaps = 2/513 (0%)

>gb|AAN80359.1|AE016760_218 (AE016760) Respiratory nitrate reductase 2 beta chain [Escherichia
           coli CFT073]
          Length = 514

 Score =  821 bits (2121), Expect = 0.0
 Identities = 380/514 (73%), Positives = 446/514 (86%), Gaps = 6/514 (1%)








 
Structural Features:
COG1140, NarY, Nitrate reductase beta subunit 
[Energy production and conversion]
CD-Length = 513 residues, 100.0% aligned
Score =  955 bits (2469), Expect = 0.0
Identities = 394/515 (76%), Positives = 450/515 (87%), Gaps = 4/515 (0%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1281




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18423




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31690




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:639_cdd.html


    		




 
URL:

















    ReferenceID:34331




  
Author/Investigator(s):
        Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V.





  
Title:
        Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.




  
PubMed:
        2674654


MedLine:
        




    
Source:
Mol Gen Genet. 1989 Aug;218(2):249-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2674654

















    ReferenceID:34332




  
Author/Investigator(s):
        Yoshimatsu K, Iwasaki T, Fujiwara T.





  
Title:
        Sequence and electron paramagnetic resonance analyses of nitrate reductase NarGH from a denitrifying halophilic euryarchaeote Haloarcula marismortui.




  
PubMed:
        11959121


MedLine:
        




    
Source:
FEBS Lett. 2002 Apr 10;516(1-3):145-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11959121









           
Homologs By Global Alignment 

           

       



     
Gene ID:639
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1086
                           PA3874_tr
                           translation of PA3874
                           513
                           99.99
                           99.99
                           0
                           2768.0
     

     
     
41306
                           gb|AAG07261.1|AE004804_9
                           (AE004804) respiratory nitrate reductase beta chain [Pseudomonas aeruginosa PAO1]
                           513
                           99.99
                           99.99
                           0
                           2768.0
     

     
     
41307
                           emb|CAA75541.1|
                           (Y15252) respiratory nitrate reductase beta subunit [Pseudomonas aeruginosa]
                           513
                           99.99
                           99.99
                           0
                           2768.0
     

     
     
41308
                           gb|AAG34374.1|AF197465_6
                           (AF197465) respiratory nitrate reductase beta subunit; NarH [Pseudomonas fluorescens]
                           514
                           85.40
                           92.21
                           3
                           2416.0
     

     
     
41309
                           dbj|BAB84313.1|
                           (AB076402) probable nitrate reductase beta subunit [Halomonas halodenitrificans]
                           532
                           76.69
                           86.65
                           23
                           2261.0
     

     






      

          
GC ORFID: 43641How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3874Glimmer Score: 

          
Start: 3766534Stop: 3764993

          
Length: 1542


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3874 translation of PA3874Homolog Bit Score 1070.0

          
Other Homologs:  t_PA3874 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAATTCGTTCGCAAGTCGGCATGGTGCTGAACCTCGACAAGTGCATTGGTTGCCACACCTGCTCGATC 
ACCTGCAAGAACGTCTGGACCAGCCGCGAAGGCATGGAGTACGCCTGGTTCAACAACGTCGAGACCAAGCCC 
GGGATCGGCTACCCGAAGGAATGGGAAAACCAGGAGAAGTGGAAGGGCGGCTGGGTGCGCGCGGCGGACGGT 
TCGATCCGCCCGCGCATCGGCGGCAAGTTCCGCGTGCTGGCGAACATCTTCGCCAACCCGGACCTGCCCGAG 
ATCGACGACTACTACGAACCGTTCGACTTCGATTACCAGCACCTGCATACCGCGCCCAAGGCCGAGCACCAG 
CCGGTGGCGCGCCCGCGCTCGCTGGTCTCCGGGCAGCGCATGGAGAAGATCGAGTGGGGCCCGAACTGGGAG 
GAGATCCTCGGCACCGAGTTCGCCAAGCGGCGCAAGGACAAGAACTTCGACCAGGTCCAGGCGGACATCTAC 
GGTGAGTACGAGAACACCTTCATGATGTACCTGCCGCGCCTCTGCGAGCACTGCCTGAACCCGGCGTGCGTG 
GCGTCCTGCCCGAGCGGGGCGATCTACAAGCGCGAGGAGGACGGCATCGTCCTGATCGACCAGGACAAGTGC 
CGCGGCTGGCGGATGTGCATCTCCGGCTGCCCGTACAAGAAGATCTACTTCAACTGGAAGAGCGGCAAGTCC 
GAGAAGTGCATCTTCTGCTACCCGCGCATCGAGGCCGGCCAGCCCACTGTCTGCTCGGAGACCTGCGTCGGG 
CGCATCCGCTACCTCGGCGTGCTGCTCTACGACGCCGACCGCATCCACGAAGTGGCCAGTTGCGAGAACGAG 
CGCGAGCTGTACGAGAAGCAACTGGAGATCTTCCTCGATCCGTTCGACCCGGCGGTGATCGCCCAGGCGCGC 
AAGGACGGGGTGGCCGACAGCGTCATCGAGGCGGCGCAGAAGTCGCCGGTGTACAAGCTGGCGATGGACTGG 
AAGCTGGCCCTGCCGCTGCACCCGGAATACCGCACGCTGCCGATGGTCTGGTACGTGCCACCGCTGTCGCCG 
ATCCAGAACGCCGCCGCCGAGGGGCACATCGGCAGCGACGGGGTGATCCCGGACGTGGAGTCGCTGCGCATC 
CCCGTGCAGTACCTGGCCAACCTGCTCACCGCCGGCGACACCGCGCCGGTGCTGCTGGCGCTCAAGCGCCTG 
CTGGCGATGCGCGCCTACAAGCGCGCCGAGCACGTCGAAGGCCGCCAGGACCTGGAGGTGCTGGCCAAGGTC 
GGGTTGAGCGTGGAGCAGGTGGAGGAGATGTACCGCTACCTGGCCATCGCCAACTACGAGGATCGCTTCGTG 
ATCCCCAGCGCGCACCGCGAGGAAGCGCTTTCCGATGCCTTCGCCGAGCGTTCCGGCTGCGGCTTCAGCTTC 
GGCAACGGCTGTTCCGGCGGCAGCAACTCCGCCGTCAACCTGTTCGGCGGCAAGCCGACCAACCGCCGCGAC 
GTGATCCAGGTCGTGCAGATCCAGGAGTGA