ViewGene
Locus Name:
GeneID:
Location:

GeneID:635
TrivialName: PA14_11000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11000
PAO1 Ortholog Locus:PA4091
Sequence Length:1563
Protein Length:520
Start: 952606
Stop: 951044
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07478.1|AE004825_7 (AE004825) 4-hydroxyphenylacetate 3-monooxygenase large chain [Pseudomonas aeruginosa PAO1]
Identities = 517/520 (99%), Positives = 519/520 (99%)
Sequence:
ATGAAACCCGAAGATTTCCGTGCCTCTGCCACCCGTCCGTTCACCGGCGAGGAGTACCTCGCCAGCCTGCGC
GACGACCGTGAGATCTACATCTACGGCGACCGCGTCAAGGACGTCACCAGCCACCCCGCCTTCCGCAACGCG
GCCGCCTCCATGGCCCGGCTCTACGACGCCCTGCACGATCCGCAGAGCAAGGAAAAGCTCTGCTGGGAGACC
GATACCGGCAACGGCGGCTATACCCACAAGTTCTTCCGCTACGCGCGCAGCGCCGACGAACTGCGCCAGCAG
CGCGACGCCATCGCCGAGTGGTCGCGGCTGACCTACGGCTGGATGGGCCGCACCCCGGACTACAAGGCCGCC
TTCGGCAGCGCCCTCGGCGCCAACCCGGGCTTCTACGGGCGTTTCGAGGACAACGCGAAAACCTGGTACAAG
CGCATCCAGGAAGCCTGCCTGTACCTCAACCATGCCATCGTCAACCCGCCGATCGACCGCGACAAGCCGGTG
GACCAGGTCAAGGACGTGTTCATCTCGGTGGACGAGGAAGTCGACGGCGGCATCGTCGTCAGCGGCGCCAAG
GTGGTCGCCACGAATTCCGCGCTGACCCACTACAACTTCGTCGGCCAGGGTTCGGCGCAACTGCTCGGCGAC
AACACCGACTTCGCCCTGATGTTCATCGCGCCGATGAACACCCCCGGCATGAAGCTGATCTGCCGCCCCTCC
TACGAACTGGTGGCGGGTATCGCCGGATCGCCGTTCGACTACCCGCTGTCCAGCCGTTTCGACGAGAACGAC
GCGATCCTGGTGATGGACAAGGTGTTCATCCCCTGGGAGAACGTACTGATCTACCGCGACTTCGAGCGCTGC
AAGCAGTGGTTCCCCCAGGGTGGCTTCGGCCGGCTGTTCCCGATGCAGGGCTGCACCCGCCTGGCGGTCAAG
CTCGACTTCATCACCGGCGCCCTCTACAAGGCCCTGCAATGCACCGGCTCCCTGGAGTTCCGCGGCGTGCAG
GCGCAGGTCGGCGAAGTGGTGGCCTGGCGCAACCTGTTCTGGTCGCTGACCGACGCCATGTACGGCAACGCC
AGCGAATGGTACAGCGGCGCCTTCCTACCCAGCGCCGAGGCCCTGCAGGCCTACCGCGTGCTGGCGCCGCAG
GCCTACCCGGAGATCAAGAAGACCATCGAGCAGGTGGTCGCCAGCGGCCTGATCTACCTGCCCTCCGGCGTT
CGCGACCTGCACAATCCGCAGCTCGACAAGTACCTCTCCACCTATTGCCGCGGCTCCGGCGGCATGGGCCAC
CGGGAGCGGATCAAGATCCTCAAGCTGCTCTGGGACGCCATCGGCAGCGAGTTCGGCGGCCGCCACGAGCTG
TACGAGATCAACTACGCCGGCAGCCAGGACGAGATCCGCATGCAGGCCCTGCGCCAGGCGGTCGGCAGCGGG
GCGATGAAGGGCATGCTCGGCATGGTCGAGCAGTGCATGGGCGACTACGACGAGAACGGCTGGACCGTGCCG
CACCTGCACAACCCGGACGACATCAACGTGCTCGACCGCATCCGCCAATGA
Translation:
MKPEDFRASATRPFTGEEYLASLRDDREIYIYGDRVKDVTSHPAFRNAAASMARLYDALHDPQSKEKLCWET
DTGNGGYTHKFFRYARSADELRQQRDAIAEWSRLTYGWMGRTPDYKAAFGSALGANPGFYGRFEDNAKTWYK
RIQEACLYLNHAIVNPPIDRDKPVDQVKDVFISVDEEVDGGIVVSGAKVVATNSALTHYNFVGQGSAQLLGD
NTDFALMFIAPMNTPGMKLICRPSYELVAGIAGSPFDYPLSSRFDENDAILVMDKVFIPWENVLIYRDFERC
KQWFPQGGFGRLFPMQGCTRLAVKLDFITGALYKALQCTGSLEFRGVQAQVGEVVAWRNLFWSLTDAMYGNA
SEWYSGAFLPSAEALQAYRVLAPQAYPEIKKTIEQVVASGLIYLPSGVRDLHNPQLDKYLSTYCRGSGGMGH
RERIKILKLLWDAIGSEFGGRHELYEINYAGSQDEIRMQALRQAVGSGAMKGMLGMVEQCMGDYDENGWTVP
HLHNPDDINVLDRIRQ*
AnnotationID:644GeneID:635
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 10:10:36
Gene Name:hpaA
Confidence Code:2
GeneProduct:4-hydroxyphenylacetate 3-monooxygenase large chain
Cell Localization:()
Synonyms:hpaB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:4-hydroxyphenylacetate 3-monooxygenase; oxygenase component
Functional Category Confidence Code:5
COGs:COG2368,pfam03241
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:1.14.13.3
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA82321.1| (Z29081) 4-hydroxyphenylacetic hydroxylase [Escherichia coli]
          Length = 520

 Score =  807 bits (2085), Expect = 0.0
 Identities = 374/518 (72%), Positives = 441/518 (85%)

>gb|AAL20031.1| (AE008747) 4-hydroxyphenylacetate catabolism protein [Salmonella
           typhimurium LT2]
          Length = 520

 Score =  807 bits (2084), Expect = 0.0
 Identities = 376/518 (72%), Positives = 440/518 (84%)

>gb|AAM86095.1|AE013856_9 (AE013856) putative component B of 4-hydroxyphenylacetic
           acid-hydroxylase [Yersinia pestis KIM]
          Length = 520

 Score =  800 bits (2067), Expect = 0.0
 Identities = 376/518 (72%), Positives = 436/518 (84%)






 
Structural Features:
COG2368, COG2368, Aromatic ring hydroxylase 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 493 residues, 100.0% aligned
Score =  668 bits (1726), Expect = 0.0
Identities = 255/499 (51%), Positives = 317/499 (63%), Gaps = 14/499 (2%)

pfam03241, HpaB, 4-hydroxyphenylacetate 3-hydroxylase family. 
CD-Length = 368 residues, 100.0% aligned
Score =  394 bits (1013), Expect = 2e-110
Identities = 154/375 (41%), Positives = 205/375 (54%), Gaps = 8/375 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1273




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18415




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36844




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:635_cdd.html


    		




 
URL:

















    ReferenceID:36945




  
Author/Investigator(s):
        Evans DG, Karjalainen TK, Evans DJ Jr, Graham DY, Lee CH.





  
Title:
        Cloning, nucleotide sequence, and expression of a gene encoding an adhesin subunit protein of Helicobacter pylori.




  
PubMed:
        7678592


MedLine:
        




    
Source:
J Bacteriol. 1993 Feb;175(3):674-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7678592

















    ReferenceID:36946




  
Author/Investigator(s):
        Prieto MA, Diaz E, Garcia JL.





  
Title:
        Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.




  
PubMed:
        8550403


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(1):111-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550403

















    ReferenceID:36949




  
Author/Investigator(s):
        Prieto MA, Garcia JL.





  
Title:
        Molecular characterization of 4-hydroxyphenylacetate 3-hydroxylase of Escherichia coli. A two-protein component enzyme.




  
PubMed:
        8077235


MedLine:
        




    
Source:
J Biol Chem. 1994 Sep 9;269(36):22823-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94357932&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:635
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1072
                           PA4091_tr
                           translation of PA4091
                           520
                           99.42
                           99.80
                           0
                           2769.0
     

     
     
41147
                           gb|AAG07478.1|AE004825_7
                           (AE004825) 4-hydroxyphenylacetate 3-monooxygenase large chain [Pseudomonas aeruginosa PAO1]
                           520
                           99.42
                           99.80
                           0
                           2769.0
     

     
     
41148
                           gb|AAR11357.1|
                           (AY392450) HpaB [Escherichia coli]
                           520
                           72.69
                           85.19
                           0
                           2096.0
     

     
     
41156
                           emb|CAC90587.1|
                           (AJ414150) 4-hydroxyphenylacetate 3-monooxygenase [Yersinia pestis CO92]
                           520
                           72.30
                           84.03
                           0
                           2067.0
     

     
     
41155
                           gb|AAM86095.1|AE013856_9
                           (AE013856) putative component B of 4-hydroxyphenylacetic acid-hydroxylase [Yersinia pestis KIM]
                           520
                           72.30
                           84.03
                           0
                           2067.0
     

     






      

          
GC ORFID: 43637How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4091Glimmer Score: 

          
Start: 4001331Stop: 4002893

          
Length: 1563


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4091 translation of PA4091Homolog Bit Score 1071.0

          
Other Homologs:  t_PA2256 (ORF: BLASTX 431.0), t_PA4091 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACCCGAAGATTTCCGTGCCTCTGCCACCCGTCCGTTCACCGGCGAGGAGTACCTCGCCAGCCTGCGC 
GACGACCGTGAGATCTACATCTACGGCGACCGCGTCAAGGACGTCACCAGCCACCCCGCCTTCCGCAACGCG 
GCCGCCTCCATGGCCCGGCTCTACGACGCCCTGCACGATCCGCAGAGCAAGGAAAAGCTCTGCTGGGAGACC 
GATACCGGCAACGGCGGCTATACCCACAAGTTCTTCCGCTACGCGCGCAGCGCCGACGAACTGCGCCAGCAG 
CGCGACGCCATCGCCGAGTGGTCGCGGCTGACCTACGGCTGGATGGGCCGCACCCCGGACTACAAGGCCGCC 
TTCGGCAGCGCCCTCGGCGCCAACCCGGGCTTCTACGGGCGTTTCGAGGACAACGCGAAAACCTGGTACAAG 
CGCATCCAGGAAGCCTGCCTGTACCTCAACCATGCCATCGTCAACCCGCCGATCGACCGCGACAAGCCGGTG 
GACCAGGTCAAGGACGTGTTCATCTCGGTGGACGAGGAAGTCGACGGCGGCATCGTCGTCAGCGGCGCCAAG 
GTGGTCGCCACGAATTCCGCGCTGACCCACTACAACTTCGTCGGCCAGGGTTCGGCGCAACTGCTCGGCGAC 
AACACCGACTTCGCCCTGATGTTCATCGCGCCGATGAACACCCCCGGCATGAAGCTGATCTGCCGCCCCTCC 
TACGAACTGGTGGCGGGTATCGCCGGATCGCCGTTCGACTACCCGCTGTCCAGCCGTTTCGACGAGAACGAC 
GCGATCCTGGTGATGGACAAGGTGTTCATCCCCTGGGAGAACGTACTGATCTACCGCGACTTCGAGCGCTGC 
AAGCAGTGGTTCCCCCAGGGTGGCTTCGGCCGGCTGTTCCCGATGCAGGGCTGCACCCGCCTGGCGGTCAAG 
CTCGACTTCATCACCGGCGCCCTCTACAAGGCCCTGCAATGCACCGGCTCCCTGGAGTTCCGCGGCGTGCAG 
GCGCAGGTCGGCGAAGTGGTGGCCTGGCGCAACCTGTTCTGGTCGCTGACCGACGCCATGTACGGCAACGCC 
AGCGAATGGTACAGCGGCGCCTTCCTACCCAGCGCCGAGGCCCTGCAGGCCTACCGCGTGCTGGCGCCGCAG 
GCCTACCCGGAGATCAAGAAGACCATCGAGCAGGTGGTCGCCAGCGGCCTGATCTACCTGCCCTCCGGCGTT 
CGCGACCTGCACAATCCGCAGCTCGACAAGTACCTCTCCACCTATTGCCGCGGCTCCGGCGGCATGGGCCAC 
CGGGAGCGGATCAAGATCCTCAAGCTGCTCTGGGACGCCATCGGCAGCGAGTTCGGCGGCCGCCACGAGCTG 
TACGAGATCAACTACGCCGGCAGCCAGGACGAGATCCGCATGCAGGCCCTGCGCCAGGCGGTCGGCAGCGGG 
GCGATGAAGGGCATGCTCGGCATGGTCGAGCAGTGCATGGGCGACTACGACGAGAACGGCTGGACCGTGCCG 
CACCTGCACAACCCGGACGACATCAACGTGCTCGACCGCATCCGCCAATGA