ViewGene
Locus Name:
GeneID:
Location:

GeneID:630
TrivialName: PA14_17290
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17290
PAO1 Ortholog Locus:PA3637
Sequence Length:1629
Protein Length:542
Start: 1485522
Stop: 1487150
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07025.1|AE004783_10 (AE004783) CTP synthase [Pseudomonas aeruginosa PAO1]
Identities = 542/542 (100%), Positives = 542/542 (100%)
Sequence:
ATGACGCGCTACATCTTCGTCACGGGTGGTGTTGTTTCTTCATTGGGGAAAGGCATCGCTTCGGCTTCTCTG
GCTGCGATCTTGGAAGCGCGTGGCCTGAAGATCACGATGCTCAAGCTGGACCCTTACATCAACGTGGACCCG
GGCACCATGAGCCCGTTCCAGCACGGTGAGGTCTTCGTGACCCAGGACGGCGCCGAGACCGACCTGGACCTG
GGCCATTACGAGCGCTTCGTTCGTACCACCATGACCCAGAACAACAACTTCACCACCGGCCGCGTCTACATG
GACGTGTTGCGCAAGGAACGCCGCGGCGACTACCTGGGCGCCACCGTGCAGGTGATCCCGCACATCACCGAC
GAGATCAAGCGGCGCATCATCAAGGGCGCCGGCGATGCCGACGTCGCCCTGGTGGAGATCGGTGGCACCGTC
GGCGACATCGAGTCGCAGCCGTTCCTCGAGGCGATCCGCCAGCTGCGCGTCGAGATCGGCGCCAAGCGCGCG
ATGCTGATGCACCTGACCCTGGTCCCGTACATCGCCACCGCCGGCGAGACCAAGACCAAGCCGACCCAGCAC
TCGGTCAAGGAGCTGCGCTCCATCGGCCTGCAACCGGACGTGCTGGTCTGCCGTTCCGACCACCCGATCGAC
GTCTCCTCGCGGCGCAAGATCGCGCTCTTCACCAACGTCGAGGAGCGCGCGGTGATCGCCCTGGAAGACGTC
GACACCATCTACCGCATCCCGTCGGTGCTGCATGCCCAGGGCCTGGACGACATCGTCGTCGAGCGCTTCGGC
CTGGAGTGCGGGCAGGCCGACCTGTCCGAATGGGACCGCGTGGTCGATGCCAAGCTCAACCCCGAGCGTGAA
GTCACCATCGCCATGGTCGGCAAGTACATGGAACTGCTGGACGCGTACAAGTCGCTGATCGAGGCGATGACC
CACGCCGGCATCCAGAGCCGCACCAAGGTCAACCTGCGCTACATCGACTCCGAAGACATCGAGCAGCAGGGC
ACCAGCCTGCTGGAAGGCGTCGACGCCATCCTGGTCCCGGGCGGCTTCGGCCTGCGCGGCGTGGAAGGCAAG
ATCAGCACCGTGCAGTACGCCCGCGAGAACAAGATCCCCTACCTGGGCATCTGCCTGGGCATGCAGGTCGCG
GTCATCGAATACGCCCGCAACGTGCTCGGCTGGAGCGACGCCAACTCCACCGAGTTCGACAAGTCCAGCGGC
CATCCGGTGGTCGGCCTGATCACCGAATGGCAGGACGCGACCGGCGCCACCGAGATCCGCACCGAAGCTTCC
GACCTGGGCGGCACCATGCGCCTGGGCGCCCAGGAGTGCCAGCTGCAGACCGGCACCCTGGTGCACGACTGC
TATGCCAAGGACGTGATCGTCGAGCGCCATCGCCATCGCTACGAAGTGAACAACAACCTGCTGCCGCAACTG
GAACAGGCCGGCCTGAAGATTTCCGGCCGCTCCGGCGACGGCGCCCTGGTGGAAGTGGTCGAGGCCCCGGAG
CATCCGTGGTTCGTTGCCTGCCAGTTCCACCCGGAATTCACCTCCACACCGCGTGATGGCCACCCGCTGTTC
AGCGGTTTCGTCAACGCCGCGCTGAAATATTCCGGGAAGGCCTGA
Translation:
MTRYIFVTGGVVSSLGKGIASASLAAILEARGLKITMLKLDPYINVDPGTMSPFQHGEVFVTQDGAETDLDL
GHYERFVRTTMTQNNNFTTGRVYMDVLRKERRGDYLGATVQVIPHITDEIKRRIIKGAGDADVALVEIGGTV
GDIESQPFLEAIRQLRVEIGAKRAMLMHLTLVPYIATAGETKTKPTQHSVKELRSIGLQPDVLVCRSDHPID
VSSRRKIALFTNVEERAVIALEDVDTIYRIPSVLHAQGLDDIVVERFGLECGQADLSEWDRVVDAKLNPERE
VTIAMVGKYMELLDAYKSLIEAMTHAGIQSRTKVNLRYIDSEDIEQQGTSLLEGVDAILVPGGFGLRGVEGK
ISTVQYARENKIPYLGICLGMQVAVIEYARNVLGWSDANSTEFDKSSGHPVVGLITEWQDATGATEIRTEAS
DLGGTMRLGAQECQLQTGTLVHDCYAKDVIVERHRHRYEVNNNLLPQLEQAGLKISGRSGDGALVEVVEAPE
HPWFVACQFHPEFTSTPRDGHPLFSGFVNAALKYSGKA*
AnnotationID:639GeneID:630
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 21:09:37
Gene Name:pyrG
Confidence Code:1
GeneProduct:CTP synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0504,KOG2387,pfam06418
Secondary Functional Category(ies):
EC Number:6.3.4.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55072.1| (AE016861) CTP synthase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 543

 Score =  989 bits (2557), Expect = 0.0
 Identities = 487/536 (90%), Positives = 513/536 (95%)

>gb|AAA24485.1| (M12843) CTP synthetase (EC 6.3.4.2) [Escherichia coli]
          Length = 545

 Score =  778 bits (2010), Expect = 0.0
 Identities = 373/537 (69%), Positives = 448/537 (83%)






 
Structural Features:
COG0504, PyrG, CTP synthase (UTP-ammonia lyase) 
[Nucleotide transport and metabolism]
CD-Length = 533 residues, 100.0% aligned
Score =  904 bits (2339), Expect = 0.0
Identities = 354/543 (65%), Positives = 424/543 (78%), Gaps = 12/543 (2%)

KOG2387, CTP synthase (UTP-ammonia lyase) 
[Nucleotide transport and metabolism]
CD-Length = 585 residues,  94.4% aligned
Score =  604 bits (1559), Expect = 2e-173
Identities = 263/563 (46%), Positives = 369/563 (65%), Gaps = 35/563 (6%)

pfam06418, CTP_synth_N, CTP synthase N-terminus. 
CD-Length = 280 residues, 100.0% aligned
Score =  494 bits (1274), Expect = 2e-140
Identities = 191/280 (68%), Positives = 234/280 (83%), Gaps = 3/280 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1263




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18405




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31906




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:630_cdd.html


    		




 
URL:

















    ReferenceID:34191




  
Author/Investigator(s):
        Walsh AG, Burrows LL, Lam JS.





  
Title:
        Genetic and biochemical characterization of an operon involved in the biosynthesis of 3-deoxy-D-manno-octulosonic acid in Pseudomonas aeruginosa.




  
PubMed:
        10220877


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 Apr 1;173(1):27-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10220877

















    ReferenceID:34192




  
Author/Investigator(s):
        Kim JH, Kim JK, Choe YK.





  
Title:
        Cloning and sequencing of the pyrG encoding cytidine 5'-triphosphate synthetase from Mycobacterium bovis BCG.




  
PubMed:
        9415801


MedLine:
        




    
Source:
Biochem Mol Biol Int. 1997 Dec;43(5):925-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9415801

















    ReferenceID:34193




  
Author/Investigator(s):
        Zimmer W, Hundeshagen B.





  
Title:
        Identification and sequencing of pyrG, the CTP synthetase gene of Azospirillum brasilense Sp7.




  
PubMed:
        8138139


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 Jan 15;115(2-3):273-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8138139

















    ReferenceID:34194




  
Author/Investigator(s):
        Weng M, Makaroff CA, Zalkin H.





  
Title:
        Nucleotide sequence of Escherichia coli pyrG encoding CTP synthetase.




  
PubMed:
        3514618


MedLine:
        




    
Source:
J Biol Chem. 1986 Apr 25;261(12):5568-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3514618









           
Homologs By Global Alignment 

           

       



     
Gene ID:630
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1067
                           PA3637_tr
                           translation of PA3637
                           542
                           99.99
                           99.99
                           0
                           2782.0
     

     
     
40950
                           gb|AAG07025.1|AE004783_10
                           (AE004783) CTP synthase [Pseudomonas aeruginosa PAO1]
                           542
                           99.99
                           99.99
                           0
                           2782.0
     

     
     
40951
                           gb|AAO55072.1|
                           (AE016861) CTP synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           543
                           89.87
                           94.65
                           1
                           2555.0
     

     
     
40952
                           gb|AAN67231.1|AE016350_4
                           (AE016779) CTP synthase [Pseudomonas putida KT2440]
                           542
                           89.29
                           94.09
                           0
                           2540.0
     

     
     
40953
                           gb|AAK03956.1|
                           (AE006225) PyrG [Pasteurella multocida]
                           543
                           71.63
                           83.42
                           2
                           2063.0
     

     






      

          
GC ORFID: 43632How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3637Glimmer Score: 

          
Start: 3468415Stop: 3466787

          
Length: 1629


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3637 translation of PA3637Homolog Bit Score 1076.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4639 (ORF: BLASTX 991.0), t_PA3637 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGCGCTACATCTTCGTCACGGGTGGTGTTGTTTCTTCATTGGGGAAAGGCATCGCTTCGGCTTCTCTG 
GCTGCGATCTTGGAAGCGCGTGGCCTGAAGATCACGATGCTCAAGCTGGACCCTTACATCAACGTGGACCCG 
GGCACCATGAGCCCGTTCCAGCACGGTGAGGTCTTCGTGACCCAGGACGGCGCCGAGACCGACCTGGACCTG 
GGCCATTACGAGCGCTTCGTTCGTACCACCATGACCCAGAACAACAACTTCACCACCGGCCGCGTCTACATG 
GACGTGTTGCGCAAGGAACGCCGCGGCGACTACCTGGGCGCCACCGTGCAGGTGATCCCGCACATCACCGAC 
GAGATCAAGCGGCGCATCATCAAGGGCGCCGGCGATGCCGACGTCGCCCTGGTGGAGATCGGTGGCACCGTC 
GGCGACATCGAGTCGCAGCCGTTCCTCGAGGCGATCCGCCAGCTGCGCGTCGAGATCGGCGCCAAGCGCGCG 
ATGCTGATGCACCTGACCCTGGTCCCGTACATCGCCACCGCCGGCGAGACCAAGACCAAGCCGACCCAGCAC 
TCGGTCAAGGAGCTGCGCTCCATCGGCCTGCAACCGGACGTGCTGGTCTGCCGTTCCGACCACCCGATCGAC 
GTCTCCTCGCGGCGCAAGATCGCGCTCTTCACCAACGTCGAGGAGCGCGCGGTGATCGCCCTGGAAGACGTC 
GACACCATCTACCGCATCCCGTCGGTGCTGCATGCCCAGGGCCTGGACGACATCGTCGTCGAGCGCTTCGGC 
CTGGAGTGCGGGCAGGCCGACCTGTCCGAATGGGACCGCGTGGTCGATGCCAAGCTCAACCCCGAGCGTGAA 
GTCACCATCGCCATGGTCGGCAAGTACATGGAACTGCTGGACGCGTACAAGTCGCTGATCGAGGCGATGACC 
CACGCCGGCATCCAGAGCCGCACCAAGGTCAACCTGCGCTACATCGACTCCGAAGACATCGAGCAGCAGGGC 
ACCAGCCTGCTGGAAGGCGTCGACGCCATCCTGGTCCCGGGCGGCTTCGGCCTGCGCGGCGTGGAAGGCAAG 
ATCAGCACCGTGCAGTACGCCCGCGAGAACAAGATCCCCTACCTGGGCATCTGCCTGGGCATGCAGGTCGCG 
GTCATCGAATACGCCCGCAACGTGCTCGGCTGGAGCGACGCCAACTCCACCGAGTTCGACAAGTCCAGCGGC 
CATCCGGTGGTCGGCCTGATCACCGAATGGCAGGACGCGACCGGCGCCACCGAGATCCGCACCGAAGCTTCC 
GACCTGGGCGGCACCATGCGCCTGGGCGCCCAGGAGTGCCAGCTGCAGACCGGCACCCTGGTGCACGACTGC 
TATGCCAAGGACGTGATCGTCGAGCGCCATCGCCATCGCTACGAAGTGAACAACAACCTGCTGCCGCAACTG 
GAACAGGCCGGCCTGAAGATTTCCGGCCGCTCCGGCGACGGCGCCCTGGTGGAAGTGGTCGAGGCCCCGGAG 
CATCCGTGGTTCGTTGCCTGCCAGTTCCACCCGGAATTCACCTCCACACCGCGTGATGGCCACCCGCTGTTC 
AGCGGTTTCGTCAACGCCGCGCTGAAATATTCCGGGAAGGCCTGA