ViewGene

Locus Name:
GeneID:
Location:

GeneID: 6269

TrivialName: PA14_23420

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_23420

PAO1 Ortholog Locus:

Sequence Length: 2169

Protein Length: 722

Start: 2034189

Stop: 2036357

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAM27713.1|AF498411_10 (AF498411) ORF_10; similar to Zinc-binding dehydrogenases [Pseudomonas aeruginosa] Identities = 722/722 (100%), Positives = 722/722 (100%)

Sequence:

ATGAGTTTCTGTATTGAGGGCGCTATGAAGCAAGTTCTTCAAGATATGGCTAAAGGTGGCACCAGTGTCACG
GTCGCTCCGGCACCGCAGATATCGCGTGATCATGTGATTATCAATAGTACTGTCTCGCTGATATCGGCGGGG
ACCGAGCGTATGTTGGTTGGCTTCGGTAAGGCATCCTACCTTGATAAGGCTCGTCAACAGCCAGAGAAGGTC
AAGATGGTCTTGGAAAAGATCAAAACCGATGGATTGATGACCACAGTAGAGGCTGTACAGTCCAAGCTCGCA
CAGCCACTTCCCCTAGGCTACTGCAATGTAGGAGTGGTGTCTGAGATCGGCTCTGGTGTTGAGGGCTTCAAG
GTTGGTGATCGAGTTGTTTCCAACGGTCCTCATGCTGATCTAGTCAAAGTGTCGAAGAACCTCTGTGCACGT
ATTCCGGATGGCGTCGATGATGAATCGGCGGCATTTGTAGTCGTGGCGAGCATTGGTCTACAGGGTATCCGT
CTTGCACAACCAACCTTGGGCGAGTGTTTTGTTGTCACAGGAGTTGGCCTCATTGGTCTACTCACTGTTCAG
TTGCTACGGGCTCATGGCTGTCGGGTGTTGGCTATTGATTTTGATGATTCGAAGCTAGAACTTGCTCGACAG
TACGGGGCGCAGACTTGTAACCCTGGTAAAGGTGAGGATCCGGTTGCTGCAGGCATGGCATTCAGCCGAGGA
AAAGGTGTCGACGGCGTAATTATCACTGCCTCAACAAAGTCCAGTGACCCTGTAACTCAGGCTGCACGCATG
AGTCGTAAGCGTGGACGGATTGTTTTGGTGGGAGTGGTGGGATTGGAGCTCAATCGCGCTGACTTTTATGAA
AAGGAGTTGACTTTTCAGGTATCGTGCTCATATGGACCGGGGCGCTATGATCCTGAATACGAGGAGAAAGGA
GGAGACTACCCTCTCGGCTTTGTCCGTTGGACTGAGCAAAGAAATTTTGAAGCTGTCCTAGACATGCTGGAT
AGCGGCCAACTGGAAGTGAAAACGTTGATTACTCATCGTTTTAAATTCGAGGACGCTCCGGCGGCTTATTCC
ACTTTAACTCAGGATAACTCTGGTCTAGGAATGCTCCTTCTGTATGCTTCGGATACGGCCGAGCGAGTTGTC
CGTCATGTCCCACTATCCGATATGAAGGGCTTCAATGCTCAAAAGCCCGTGGTTGGTTTCATTGGAGCGGGA
AATTACGCGTCTCGTATTCTGATCCCTGCCTTCAAGACGGCTGGAGCGCAATTCCATACTCTCGTTACCTCC
GGCGGGATCAATAGTGTTATTCATGGTGAAAAAGCTGGATTCTCACAGGCATCAACCAATATTGATGCAGTG
CTGAACGAGCGGATGATCAATACCATCGCTATTGTGACCCGACACGATACCCATGCGCGTTTAGTTGTCGAT
GCATTGCGCACCGGGAAGAACGTTTTCGTGGAAAAGCCTCTGGCTATCACCCATGCGGAGTTGGAGGATGTA
CAAGCAGTCCATGCTATTACGCATCAGACCGGTGGTGGTCCGCAATTGATGGTTGGTTTCAATCGGCGTTTT
GCTCCCCATATACAGAAAATGAAGGGCTTGTTGGCGTCTGTAAGGGAGCCTAAGTCCTTCATCATGACGATG
AATGCAGGCGCAATTCCGGTGGGTCATTGGACTCAAGATATTGCTGTAGGTGGCGGCAGAATCATAGGTGAA
GCATGCCACTTCATCGATTTGATGCGCTACCTGGCAGGAAGCCGGATAGTTTCGGTTCAGGCACGACGCATG
GGAGATTCCAACGCAATTGATACTTCTGAGGACAAGGCTTCGATTACTCTAGGTTTTGAGGATGGTTCCTTC
GGTACTATTCTCTACTTGGCAAACGGTGCAAGTAGTTTCCCCAAGGAGCGTATTGAGGTCTTTACTGCGGGG
CGAGTTTTGCAATTGGATAATTTTAGAAAGCTGAAAGGGTATGGTTGGCCAGGTTTCAACAAGATGAATCTA
TGGAAGCAGGACAAAGGCCAGCGGCAGTGTGTTGCGGCGTTCCTTGATTCAATTGAGAAAGGTGGAGAGCCG
ACTATCGCTTGTGAAGAAATTTTCGAGGTGACGCGAGTCTCTATAGAGGCGGCAGAGATACTGCGTAATCAA
TGGGATTGA

Translation:

MSFCIEGAMKQVLQDMAKGGTSVTVAPAPQISRDHVIINSTVSLISAGTERMLVGFGKASYLDKARQQPEKV
KMVLEKIKTDGLMTTVEAVQSKLAQPLPLGYCNVGVVSEIGSGVEGFKVGDRVVSNGPHADLVKVSKNLCAR
IPDGVDDESAAFVVVASIGLQGIRLAQPTLGECFVVTGVGLIGLLTVQLLRAHGCRVLAIDFDDSKLELARQ
YGAQTCNPGKGEDPVAAGMAFSRGKGVDGVIITASTKSSDPVTQAARMSRKRGRIVLVGVVGLELNRADFYE
KELTFQVSCSYGPGRYDPEYEEKGGDYPLGFVRWTEQRNFEAVLDMLDSGQLEVKTLITHRFKFEDAPAAYS
TLTQDNSGLGMLLLYASDTAERVVRHVPLSDMKGFNAQKPVVGFIGAGNYASRILIPAFKTAGAQFHTLVTS
GGINSVIHGEKAGFSQASTNIDAVLNERMINTIAIVTRHDTHARLVVDALRTGKNVFVEKPLAITHAELEDV
QAVHAITHQTGGGPQLMVGFNRRFAPHIQKMKGLLASVREPKSFIMTMNAGAIPVGHWTQDIAVGGGRIIGE
ACHFIDLMRYLAGSRIVSVQARRMGDSNAIDTSEDKASITLGFEDGSFGTILYLANGASSFPKERIEVFTAG
RVLQLDNFRKLKGYGWPGFNKMNLWKQDKGQRQCVAAFLDSIEKGGEPTIACEEIFEVTRVSIEAAEILRNQ
WD*

AnnotationID:6270 GeneID:6269

AnnotatorUID: danlee

Modification Date/Time: 2005-10-14 12:12:30

Gene Name: ORF_10

Confidence Code: 3

GeneProduct: similar to Zinc-binding dehydrogenases

Cell Localization: ()

Synonyms:

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (7) Cell wall / LPS / capsule

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs:

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

O antigen biosynthesis

Homology:

gi|20559950|gb|AAM27713.1|  ORF_10; similar to Zinc-binding dehydrogenases [Pseudomonas aeruginosa]
gi|20559783|gb|AAM27562.1|  ORF_10; similar to Zinc-binding dehydrogenases [Pseudomonas aeruginosa]
     Length=722
     Score = 1427 bits (3693),  Expect = 0.0
     Identities = 722/722 (100%), Positives = 722/722 (100%), Gaps = 0/722 (0%)

Structural Features:

COG0673, MviM, Predicted dehydrogenases and related proteins [General function prediction only].
             CD-Length = 342 residues,  96.2% aligned
             Score =  147 bits (371), Expect = 5e-36

COG1063, Tdh, Threonine dehydrogenase and related Zn-dependent dehydrogenases [Amino acid 
transport and metabolism / General function prediction only].
             CD-Length = 350 residues,  99.7% aligned
             Score =  129 bits (325), Expect = 1e-30

Genomic Context:

Part of variable O-antigen biosynthetic locus in P. aeruginosa.

Comment:

Identical to ORF_10 found in P. aeruginosa serotypes 010 and 019.

ReferenceID:12384

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:29655

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:31522

Author/Investigator(s): Raymond CK, Sims EH, Kas A, Spencer DH, Kutyavin TV, Ivey RG, Zhou Y, Kaul R, Clendenning JB, Olson MV.

Title: Genetic variation at the O-antigen biosynthetic locus in Pseudomonas aeruginosa.

PubMed: 12057956

MedLine: 22053227

Source:

J Bacteriol. 2002 Jul;184(13):3614-22.

Reference Type: Journal

Data:

URL:	`http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=22053227&dopt=Citation`

Homologs By Global Alignment

Gene ID:6269

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

198783 gb|AAM27713.1|AF498411_10 (AF498411) ORF_10; similar to Zinc-binding dehydrogenases [Pseudomonas aeruginosa] 722 99.99 99.99 0 3693.0

198784 gb|AAM27562.1|AF498401_10 (AF498401) ORF_10; similar to Zinc-binding dehydrogenases [Pseudomonas aeruginosa] 722 99.99 99.99 0 3693.0

198785 gb|AAM27769.1|AF498414_11 (AF498414) ORF_11; similar to Zinc-binding dehydrogenases [Pseudomonas aeruginosa] 724 77.07 85.91 13 2906.0

198786 gb|AAM27648.1|AF498407_10 (AF498407) ORF_11; similar to Zinc-binding dehydrogenases [Pseudomonas aeruginosa] 724 77.07 85.91 13 2906.0

198787 dbj|BAC93121.1| (AP005331) putatve zinc-binding dehydrogenase [Vibrio vulnificus YJ016] 722 72.71 83.24 9 2746.0

GC ORFID: `49271`	How Found: `Glimmer`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name:	Glimmer Score:
Start: `2919742`	Stop: `2917574`
Length: `2169`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog:	Homolog Bit Score
Other Homologs:
GC ORF Sequence
ATGAGTTTCTGTATTGAGGGCGCTATGAAGCAAGTTCTTCAAGATATGGCTAAAGGTGGCACCAGTGTCACG GTCGCTCCGGCACCGCAGATATCGCGTGATCATGTGATTATCAATAGTACTGTCTCGCTGATATCGGCGGGG ACCGAGCGTATGTTGGTTGGCTTCGGTAAGGCATCCTACCTTGATAAGGCTCGTCAACAGCCAGAGAAGGTC AAGATGGTCTTGGAAAAGATCAAAACCGATGGATTGATGACCACAGTAGAGGCTGTACAGTCCAAGCTCGCA CAGCCACTTCCCCTAGGCTACTGCAATGTAGGAGTGGTGTCTGAGATCGGCTCTGGTGTTGAGGGCTTCAAG GTTGGTGATCGAGTTGTTTCCAACGGTCCTCATGCTGATCTAGTCAAAGTGTCGAAGAACCTCTGTGCACGT ATTCCGGATGGCGTCGATGATGAATCGGCGGCATTTGTAGTCGTGGCGAGCATTGGTCTACAGGGTATCCGT CTTGCACAACCAACCTTGGGCGAGTGTTTTGTTGTCACAGGAGTTGGCCTCATTGGTCTACTCACTGTTCAG TTGCTACGGGCTCATGGCTGTCGGGTGTTGGCTATTGATTTTGATGATTCGAAGCTAGAACTTGCTCGACAG TACGGGGCGCAGACTTGTAACCCTGGTAAAGGTGAGGATCCGGTTGCTGCAGGCATGGCATTCAGCCGAGGA AAAGGTGTCGACGGCGTAATTATCACTGCCTCAACAAAGTCCAGTGACCCTGTAACTCAGGCTGCACGCATG AGTCGTAAGCGTGGACGGATTGTTTTGGTGGGAGTGGTGGGATTGGAGCTCAATCGCGCTGACTTTTATGAA AAGGAGTTGACTTTTCAGGTATCGTGCTCATATGGACCGGGGCGCTATGATCCTGAATACGAGGAGAAAGGA GGAGACTACCCTCTCGGCTTTGTCCGTTGGACTGAGCAAAGAAATTTTGAAGCTGTCCTAGACATGCTGGAT AGCGGCCAACTGGAAGTGAAAACGTTGATTACTCATCGTTTTAAATTCGAGGACGCTCCGGCGGCTTATTCC ACTTTAACTCAGGATAACTCTGGTCTAGGAATGCTCCTTCTGTATGCTTCGGATACGGCCGAGCGAGTTGTC CGTCATGTCCCACTATCCGATATGAAGGGCTTCAATGCTCAAAAGCCCGTGGTTGGTTTCATTGGAGCGGGA AATTACGCGTCTCGTATTCTGATCCCTGCCTTCAAGACGGCTGGAGCGCAATTCCATACTCTCGTTACCTCC GGCGGGATCAATAGTGTTATTCATGGTGAAAAAGCTGGATTCTCACAGGCATCAACCAATATTGATGCAGTG CTGAACGAGCGGATGATCAATACCATCGCTATTGTGACCCGACACGATACCCATGCGCGTTTAGTTGTCGAT GCATTGCGCACCGGGAAGAACGTTTTCGTGGAAAAGCCTCTGGCTATCACCCATGCGGAGTTGGAGGATGTA CAAGCAGTCCATGCTATTACGCATCAGACCGGTGGTGGTCCGCAATTGATGGTTGGTTTCAATCGGCGTTTT GCTCCCCATATACAGAAAATGAAGGGCTTGTTGGCGTCTGTAAGGGAGCCTAAGTCCTTCATCATGACGATG AATGCAGGCGCAATTCCGGTGGGTCATTGGACTCAAGATATTGCTGTAGGTGGCGGCAGAATCATAGGTGAA GCATGCCACTTCATCGATTTGATGCGCTACCTGGCAGGAAGCCGGATAGTTTCGGTTCAGGCACGACGCATG GGAGATTCCAACGCAATTGATACTTCTGAGGACAAGGCTTCGATTACTCTAGGTTTTGAGGATGGTTCCTTC GGTACTATTCTCTACTTGGCAAACGGTGCAAGTAGTTTCCCCAAGGAGCGTATTGAGGTCTTTACTGCGGGG CGAGTTTTGCAATTGGATAATTTTAGAAAGCTGAAAGGGTATGGTTGGCCAGGTTTCAACAAGATGAATCTA TGGAAGCAGGACAAAGGCCAGCGGCAGTGTGTTGCGGCGTTCCTTGATTCAATTGAGAAAGGTGGAGAGCCG ACTATCGCTTGTGAAGAAATTTTCGAGGTGACGCGAGTCTCTATAGAGGCGGCAGAGATACTGCGTAATCAA TGGGATTGA