ViewGene
Locus Name:
GeneID:
Location:

GeneID:622
TrivialName: PA14_26530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_26530
PAO1 Ortholog Locus:PA2903
Sequence Length:1680
Protein Length:559
Start: 2310997
Stop: 2312676
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06291.1|AE004716_9 (AE004716) precorrin-3 methylase CobJ [Pseudomonas aeruginosa PAO1]
Identities = 555/559 (99%), Positives = 556/559 (99%)
Sequence:
GTGAGTGACGCCCAGCAGGTTCCGGCCATCGTCATCCTCGGCCAGGGCGCGCTGGATACCGCGCGGCGGGTC
CAGGCGCGCTATCCCGGGGCGCTGGTCCACGGGCTCGCCGGGCGGGTCGAGGCCGACCGTTCCTATACGGAC
TTCGGCGACACCCTGCGCGAACTGTATTGCGCCGACCGCCCGATCGTCGCCCTGTGCGCCGCCGGCATCGTC
ATTCGCAGCCTGGCGCCGTTGTTGCAACGCAAGGGAGCGGAGCCGCCGGTGCTGGCCCTGGCCGAGGACGGC
AGCGCGGTGGTGCCGCTGCTCGGCGGGCTGGCCGGGGTCAACCGGCTGGCGCGGGAAATCGGCGAGGTGCTG
GCCGTGGCGCCGGCGATCACCACCAGCGGCGAGCTGCGCTTCGGCACCTGCGTGCTCAACCCGCCCGCCGGT
TATGTCCTGGCCGACCTGGAGCAGGGCAAGCGCTTCGTTGCCGATTTGCTCGGTGGCCAGCCGGTGCGCGTC
GAGGGTGCCGCCGACTGGCTCGACGCGGCCCGTTTGCCGCGCGACCCGGAGGCAGCGCTGGCGATTCACGTG
ACGCCCTCGGCGCGTGCGCCGCGGGCCGAGGAACTGCTGATCCATCCGCGCTGCGTGCTGGCGGCGCTCGAA
CCTGCCGACGCGGCGGCGGACGCGGTGCGCCAGGCGCTGGTCGATGCCGGCCTGGCCGCGGCGGCGCTCGGC
GGCTTGCTGGCCGACAGCGCGCGGATGGCCGATCCGCGCCTGCAGGCCGCCGCCGATGCGCTCGGCGTACCG
CTGCGCTTCCTGCCGCAGACCGATCCCGCTGCGCGCCTGCGCCAGGCCTTGCCCGCCGCCGAGCAGGTCGGC
GACCGGCTGGCGGTGGCCGCCGCGCCCCTGGCGATGGACCGCATCGGCCAGCGTCGCGGCCGACTCACGGTG
ATCGGCCTGGGGCCCGGCTGCGCCGAGCTGATGGTGCCTGCGGCGCGGCGGGCGCTGGACGAGGCCGAGGAC
ATCCTCGGCTACCAGACCTACGTCGACATGGCCGGACCCTTCCGCGCCGGACAGGTGCTGCATGGCAGCGAC
AACCGCGAGGAATTGCAGCGCGCCCGGCATGCCTTCGAACTGGCGGCGACCGGCAGGGCGGTGGTGTTGGTA
TCGTCCGGCGACCCCGGCGTGTTCGCCATGGCCGCCGCGGTGCTGGAAGCCCTGCATGGCGCTGGCGAGCCG
GCCTGGGACGCGGTCGAGCTGCGGATCTTCCCTGGCGTCTCGGCGGCCCTGGCCACCGCGGCCAGGGCGGGG
GCGCCTCTGGGCCACGATTTCTGCCTGCTGTCGCTGTCCGACAACCTCAAGCCCTGGGAGGTGATCGAGCGG
CGCCTGGACCTGGCCGGCGCCGCCGACCTGGCGATGGCTTTCTACAATCCGATCTCGCGGGCGCGGCCCTGG
CAACTGGGACGGGCGCTGGAGATCGTCGCCCGCCATCGTTCGCCGCAGACCCTGGTGGTGCTCGGTCGCGAC
ATCGGGCGGCCCGGTGAGCGCCTGCTGCGCACCACCCTGGGCGAGCTGCGTGCGGAGCAGGTGGACATGCGC
ACCCTGGTGATCATCGGCTCGTCCACCACCTGCAGCTTCCCGCGTGCCGACGGCGAAGCCTGGGTCTACACG
CCGCGCTGGTACCCATCGGAATAG
Translation:
MSDAQQVPAIVILGQGALDTARRVQARYPGALVHGLAGRVEADRSYTDFGDTLRELYCADRPIVALCAAGIV
IRSLAPLLQRKGAEPPVLALAEDGSAVVPLLGGLAGVNRLAREIGEVLAVAPAITTSGELRFGTCVLNPPAG
YVLADLEQGKRFVADLLGGQPVRVEGAADWLDAARLPRDPEAALAIHVTPSARAPRAEELLIHPRCVLAALE
PADAAADAVRQALVDAGLAAAALGGLLADSARMADPRLQAAADALGVPLRFLPQTDPAARLRQALPAAEQVG
DRLAVAAAPLAMDRIGQRRGRLTVIGLGPGCAELMVPAARRALDEAEDILGYQTYVDMAGPFRAGQVLHGSD
NREELQRARHAFELAATGRAVVLVSSGDPGVFAMAAAVLEALHGAGEPAWDAVELRIFPGVSAALATAARAG
APLGHDFCLLSLSDNLKPWEVIERRLDLAGAADLAMAFYNPISRARPWQLGRALEIVARHRSPQTLVVLGRD
IGRPGERLLRTTLGELRAEQVDMRTLVIIGSSTTCSFPRADGEAWVYTPRWYPSE*
AnnotationID:631GeneID:622
AnnotatorUID: diggins
Modification Date/Time: 2005-04-07 18:06:19
Gene Name:cobJ
Confidence Code:2
GeneProduct:precorrin-3 methylase CobJ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:CbiG protein/precorrin-3B C17-methyltransferase
Functional Category Confidence Code:5
COGs:COG1010
Secondary Functional Category(ies):
EC Number:2.1.1.131
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58303.1| (AE016873) CbiG protein/precorrin-3B C17-methyltransferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 567

 Score =  699 bits (1803), Expect = 0.0
 Identities = 368/556 (66%), Positives = 429/556 (77%), Gaps = 7/556 (1%)

>gb|AAN70395.1|AE016681_4 (AE016792) precorrin-3B C17-methyltransferase [Pseudomonas putida
           KT2440]
          Length = 565

 Score =  687 bits (1773), Expect = 0.0
 Identities = 360/556 (64%), Positives = 426/556 (76%), Gaps = 7/556 (1%)

>gb|AAN30207.1|AE014427_4 (AE014427) cbiG protein/precorrin-3B C17-methyltransferase
           [Brucella suis 1330]
          Length = 581

 Score =  596 bits (1536), Expect = e-169
 Identities = 317/561 (56%), Positives = 392/561 (69%), Gaps = 7/561 (1%)






 
Structural Features:
COG1010, CobJ, Precorrin-3B methylase [Coenzyme metabolism]
CD-Length = 249 residues,  98.4% aligned
Score =  322 bits (827), Expect = 6e-89
Identities = 141/255 (55%), Positives = 177/255 (69%), Gaps = 10/255 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14181




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18389




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36152




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:622_cdd.html


    		




 
URL:

















    ReferenceID:36411




  
Author/Investigator(s):
        Crouzet J, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Thibaut D, Debussche L.





  
Title:
        Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid.




  
PubMed:
        2211521


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5980-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2211521









           
Homologs By Global Alignment 

           

       



     
Gene ID:622
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1057
                           PA2903_tr
                           translation of PA2903
                           559
                           99.28
                           99.46
                           0
                           2795.0
     

     
     
40799
                           gb|AAG06291.1|AE004716_9
                           (AE004716) precorrin-3 methylase CobJ [Pseudomonas aeruginosa PAO1]
                           559
                           99.10
                           99.46
                           0
                           2792.0
     

     
     
40800
                           gb|AAO58303.1|
                           (AE016873) CbiG protein/precorrin-3B C17-methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           574
                           64.63
                           75.60
                           22
                           1810.0
     

     
     
40801
                           gb|AAN70395.1|AE016681_4
                           (AE016792) precorrin-3B C17-methyltransferase [Pseudomonas putida KT2440]
                           567
                           63.66
                           76.01
                           10
                           1779.0
     

     
     
40803
                           gb|AAL51893.1|
                           (AE009513) CBIG PROTEIN / PRECORRIN-3B C17-METHYLTRANSFERASE [Brucella melitensis 16M]
                           569
                           55.53
                           69.24
                           11
                           1541.0
     

     






      

          
GC ORFID: 43624How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2903Glimmer Score: 

          
Start: 2642929Stop: 2641250

          
Length: 1680


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2903 translation of PA2903Homolog Bit Score 1081.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5299 (ORF: BLASTX 704.0), t_PA2903 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAGTGACGCCCAGCAGGTTCCGGCCATCGTCATCCTCGGCCAGGGCGCGCTGGATACCGCGCGGCGGGTC 
CAGGCGCGCTATCCCGGGGCGCTGGTCCACGGGCTCGCCGGGCGGGTCGAGGCCGACCGTTCCTATACGGAC 
TTCGGCGACACCCTGCGCGAACTGTATTGCGCCGACCGCCCGATCGTCGCCCTGTGCGCCGCCGGCATCGTC 
ATTCGCAGCCTGGCGCCGTTGTTGCAACGCAAGGGAGCGGAGCCGCCGGTGCTGGCCCTGGCCGAGGACGGC 
AGCGCGGTGGTGCCGCTGCTCGGCGGGCTGGCCGGGGTCAACCGGCTGGCGCGGGAAATCGGCGAGGTGCTG 
GCCGTGGCGCCGGCGATCACCACCAGCGGCGAGCTGCGCTTCGGCACCTGCGTGCTCAACCCGCCCGCCGGT 
TATGTCCTGGCCGACCTGGAGCAGGGCAAGCGCTTCGTTGCCGATTTGCTCGGTGGCCAGCCGGTGCGCGTC 
GAGGGTGCCGCCGACTGGCTCGACGCGGCCCGTTTGCCGCGCGACCCGGAGGCAGCGCTGGCGATTCACGTG 
ACGCCCTCGGCGCGTGCGCCGCGGGCCGAGGAACTGCTGATCCATCCGCGCTGCGTGCTGGCGGCGCTCGAA 
CCTGCCGACGCGGCGGCGGACGCGGTGCGCCAGGCGCTGGTCGATGCCGGCCTGGCCGCGGCGGCGCTCGGC 
GGCTTGCTGGCCGACAGCGCGCGGATGGCCGATCCGCGCCTGCAGGCCGCCGCCGATGCGCTCGGCGTACCG 
CTGCGCTTCCTGCCGCAGACCGATCCCGCTGCGCGCCTGCGCCAGGCCTTGCCCGCCGCCGAGCAGGTCGGC 
GACCGGCTGGCGGTGGCCGCCGCGCCCCTGGCGATGGACCGCATCGGCCAGCGTCGCGGCCGACTCACGGTG 
ATCGGCCTGGGGCCCGGCTGCGCCGAGCTGATGGTGCCTGCGGCGCGGCGGGCGCTGGACGAGGCCGAGGAC 
ATCCTCGGCTACCAGACCTACGTCGACATGGCCGGACCCTTCCGCGCCGGACAGGTGCTGCATGGCAGCGAC 
AACCGCGAGGAATTGCAGCGCGCCCGGCATGCCTTCGAACTGGCGGCGACCGGCAGGGCGGTGGTGTTGGTA 
TCGTCCGGCGACCCCGGCGTGTTCGCCATGGCCGCCGCGGTGCTGGAAGCCCTGCATGGCGCTGGCGAGCCG 
GCCTGGGACGCGGTCGAGCTGCGGATCTTCCCTGGCGTCTCGGCGGCCCTGGCCACCGCGGCCAGGGCGGGG 
GCGCCTCTGGGCCACGATTTCTGCCTGCTGTCGCTGTCCGACAACCTCAAGCCCTGGGAGGTGATCGAGCGG 
CGCCTGGACCTGGCCGGCGCCGCCGACCTGGCGATGGCTTTCTACAATCCGATCTCGCGGGCGCGGCCCTGG 
CAACTGGGACGGGCGCTGGAGATCGTCGCCCGCCATCGTTCGCCGCAGACCCTGGTGGTGCTCGGTCGCGAC 
ATCGGGCGGCCCGGTGAGCGCCTGCTGCGCACCACCCTGGGCGAGCTGCGTGCGGAGCAGGTGGACATGCGC 
ACCCTGGTGATCATCGGCTCGTCCACCACCTGCAGCTTCCCGCGTGCCGACGGCGAAGCCTGGGTCTACACG 
CCGCGCTGGTACCCATCGGAATAG