ViewGene
Locus Name:
GeneID:
Location:

GeneID:617
TrivialName: PA14_18500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18500
PAO1 Ortholog Locus:PA3545
Sequence Length:1632
Protein Length:543
Start: 1588191
Stop: 1586560
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06933.1|AE004775_4 (AE004775) alginate-c5-mannuronan-epimerase AlgG [Pseudomonas aeruginosa PAO1]
Identities = 542/543 (99%), Positives = 543/543 (100%)
Sequence:
ATGCCCGACATTTCCCTTTCGATCCCCCGCCGCCGGCTCCCGCGCCTGCGCCCGCTGGCCGCGGCAGTGCTC
GGCGCGGTGCTGCTGCACGGCCAGGCGTGGGCGGCGCAGCCGGTGGAGAAACCGCAGCCGGTCCCGGCGCAG
GCCGGCAACGAGCCGGGCCTGACCCAGGGCCTGAAGGAAACCGGCAACTACACGGTGACCACCGCGCCGGCC
GAACCGTTGCACCTGGACCCGCCGAAACTGCCCGACCTCTCCGGCTACACCGCCGCCGCGGTGGAAGCCAAG
ATCGTGCGCAAGCCGGGTGGCCGCGCCAGCGTGCAGCGGATGGTCCAGCAGCAGCCGCTGAAGGAGTTCACC
GGTGGCAGCAACCGCCTCTCCGAATGGGTCAAGCGCCAGCGCCAGATGCCCCAGGCGATCTTCATCGAAGGC
GGCTACGTGAACCTCGCCCAGCTCGCCGGCAAGCTTCCCGCCAGCGCGCTGGAACAGGTCGAGCCGGGGGTG
TTCGTCGCCCGCCTGCCGATCGTGGTCAGCCAGGGCGCGACCCTCGACATCGACAAGCAGGTCAAGGAACTG
CGCCTGTCCCAGGAGCGCGGCGCGTTCCTGGTCAACGACGGCATGCTGTTCGTCCGCGACAGCAAGGTCACC
GGCTGGAGCGAATCGAAGAAGGAGCCGGCCTGGTTCAAGACGCCCAACGAGTTCCGTCCGTTCCTGATCTCC
TGGGGCGGCGCCGAGGTGTACCTGAGCAACAGCACCTTCACCAGCTTCGGCTACAACGCCTCCAAGGCCTAC
GGCATCTCCATCTCGCAGTACAGCCCGGGCATGGACAAGCAGATGAAGCGCCCGCGCCCCAAGGGCTGGGTG
ATCGACTCGACCATCGTCGACAGCTGGTACGGCTTCTATTGCTACGAGGCCGACGACCTGGTGGTGAAGGGC
AACACCTACCGCGACAACATCGTCTACGGCATCGACCCGCACGACCGTTCGCACCGCCTGATCATCGCCGAC
AACACCGTCCACGGGACCAGGAAGAAGCACGGCATCATCGTCTCGCGGGAAGTCAACGACAGCTTCATCTTC
AACAACCGCAGCTACGAGAACAAGCTTTCCGGGATCGTCCTCGACCGTAACAGCGAGGGCAACCTGGTGGCC
TACAACGAGGTCTACCGCAACCATTCCGACGGCATCACCCTCTACGAGAGCGGCGACAACCTGCTCTGGGGC
AACCAGGTCCTGGCCAACCGCCGCCACGGCATCCGCGTGCGCAACAGCGTGAACATCCGCCTCTACGAAAAC
CTCGCGGCGGGCAACCAGCTGATCGGCGTGTACGGCCACATCAAGGACCTCACCAACACCGACCGCAACATC
GCCCTCGACCCGTTCGACACCAAGGTCTCGCTGATCGTGGTCGGCGGCAAGCTCGCCGGCAACGGCTCCGGC
CCGCTCTCGGTGGATTCGCCGCTGAGCCTCGAACTGTACCGGGTGGCGATGCTCGCCCCGACCAAATCCTCC
GGCATCAGCCTGCCCGGCGTTCTCGGCGAGAAGCAGGACCAGATCCTCGACCTGCTGGTGCGCCAGGACAAG
GCGGTGCTGATCGATCCCGTGGAAAGCCAGGCCGAACTCCAGGACTGA
Translation:
MPDISLSIPRRRLPRLRPLAAAVLGAVLLHGQAWAAQPVEKPQPVPAQAGNEPGLTQGLKETGNYTVTTAPA
EPLHLDPPKLPDLSGYTAAAVEAKIVRKPGGRASVQRMVQQQPLKEFTGGSNRLSEWVKRQRQMPQAIFIEG
GYVNLAQLAGKLPASALEQVEPGVFVARLPIVVSQGATLDIDKQVKELRLSQERGAFLVNDGMLFVRDSKVT
GWSESKKEPAWFKTPNEFRPFLISWGGAEVYLSNSTFTSFGYNASKAYGISISQYSPGMDKQMKRPRPKGWV
IDSTIVDSWYGFYCYEADDLVVKGNTYRDNIVYGIDPHDRSHRLIIADNTVHGTRKKHGIIVSREVNDSFIF
NNRSYENKLSGIVLDRNSEGNLVAYNEVYRNHSDGITLYESGDNLLWGNQVLANRRHGIRVRNSVNIRLYEN
LAAGNQLIGVYGHIKDLTNTDRNIALDPFDTKVSLIVVGGKLAGNGSGPLSVDSPLSLELYRVAMLAPTKSS
GISLPGVLGEKQDQILDLLVRQDKAVLIDPVESQAELQD*
AnnotationID:626GeneID:617
AnnotatorUID: danlee
Modification Date/Time: 2006-03-11 16:04:01
Gene Name:algG
Confidence Code:1
GeneProduct:alginate-c5-mannuronan-epimerase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:alginate biosynthesis protein AlgG
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):Adaptation, protection; Cell wall / LPS / capsule
EC Number:5.1.3.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66907.1|AE016319_3 (AE016778) alginate-C5-mannuronan-epimerase AlgG, putative
           [Pseudomonas putida KT2440]
          Length = 557

 Score =  704 bits (1817), Expect = 0.0
 Identities = 336/497 (67%), Positives = 409/497 (82%)

>emb|CAA61231.1| (X87973) epimerase [Azotobacter vinelandii]
          Length = 525

 Score =  688 bits (1776), Expect = 0.0
 Identities = 346/523 (66%), Positives = 413/523 (78%), Gaps = 16/523 (3%)

>gb|AAO54763.1| (AE016860) alginate biosynthesis protein AlgG [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 536

 Score =  656 bits (1692), Expect = 0.0
 Identities = 318/529 (60%), Positives = 403/529 (76%), Gaps = 1/529 (0%)






 
Structural Features:






   
Genomic Context:






   
Comment:


















    ReferenceID:1237




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18379




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32021




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:617_cdd.html


    		




 
URL:

















    ReferenceID:34131




  
Author/Investigator(s):
        Chitnis CE, Ohman DE.





  
Title:
        Cloning of Pseudomonas aeruginosa algG, which controls alginate structure.




  
PubMed:
        2160929


MedLine:
        




    
Source:
J Bacteriol. 1990 Jun;172(6):2894-900.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2160929

















    ReferenceID:34132




  
Author/Investigator(s):
        Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE.





  
Title:
        Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase.




  
PubMed:
        8144447


MedLine:
        




    
Source:
J Bacteriol. 1994 Apr;176(7):1821-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8144447









           
Homologs By Global Alignment 

           

       



     
Gene ID:617
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1048
                           PA3545_tr
                           translation of PA3545
                           543
                           99.81
                           99.99
                           0
                           2805.0
     

     
     
40721
                           gb|AAG06933.1|AE004775_4
                           (AE004775) alginate-c5-mannuronan-epimerase AlgG [Pseudomonas aeruginosa PAO1]
                           543
                           99.81
                           99.99
                           0
                           2805.0
     

     
     
40722
                           gb|AAA91125.1|
                           (U27829) alginate-C5-mannuronan-epimerase [Pseudomonas aeruginosa]
                           543
                           99.81
                           99.99
                           0
                           2805.0
     

     
     
40725
                           emb|CAA61231.1|
                           (X87973) epimerase [Azotobacter vinelandii]
                           548
                           64.05
                           77.37
                           28
                           1791.0
     

     
     
40723
                           gb|AAP46694.1|
                           (AF527790) AlgG [Pseudomonas fluorescens]
                           553
                           62.92
                           75.40
                           34
                           1831.0
     

     






      

          
GC ORFID: 43619How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3545Glimmer Score: 

          
Start: 3365744Stop: 3367375

          
Length: 1632


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3545 translation of PA3545Homolog Bit Score 1085.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4996 (ORF: BLASTX 672.0), t_PA3545 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCGACATTTCCCTTTCGATCCCCCGCCGCCGGCTCCCGCGCCTGCGCCCGCTGGCCGCGGCAGTGCTC 
GGCGCGGTGCTGCTGCACGGCCAGGCGTGGGCGGCGCAGCCGGTGGAGAAACCGCAGCCGGTCCCGGCGCAG 
GCCGGCAACGAGCCGGGCCTGACCCAGGGCCTGAAGGAAACCGGCAACTACACGGTGACCACCGCGCCGGCC 
GAACCGTTGCACCTGGACCCGCCGAAACTGCCCGACCTCTCCGGCTACACCGCCGCCGCGGTGGAAGCCAAG 
ATCGTGCGCAAGCCGGGTGGCCGCGCCAGCGTGCAGCGGATGGTCCAGCAGCAGCCGCTGAAGGAGTTCACC 
GGTGGCAGCAACCGCCTCTCCGAATGGGTCAAGCGCCAGCGCCAGATGCCCCAGGCGATCTTCATCGAAGGC 
GGCTACGTGAACCTCGCCCAGCTCGCCGGCAAGCTTCCCGCCAGCGCGCTGGAACAGGTCGAGCCGGGGGTG 
TTCGTCGCCCGCCTGCCGATCGTGGTCAGCCAGGGCGCGACCCTCGACATCGACAAGCAGGTCAAGGAACTG 
CGCCTGTCCCAGGAGCGCGGCGCGTTCCTGGTCAACGACGGCATGCTGTTCGTCCGCGACAGCAAGGTCACC 
GGCTGGAGCGAATCGAAGAAGGAGCCGGCCTGGTTCAAGACGCCCAACGAGTTCCGTCCGTTCCTGATCTCC 
TGGGGCGGCGCCGAGGTGTACCTGAGCAACAGCACCTTCACCAGCTTCGGCTACAACGCCTCCAAGGCCTAC 
GGCATCTCCATCTCGCAGTACAGCCCGGGCATGGACAAGCAGATGAAGCGCCCGCGCCCCAAGGGCTGGGTG 
ATCGACTCGACCATCGTCGACAGCTGGTACGGCTTCTATTGCTACGAGGCCGACGACCTGGTGGTGAAGGGC 
AACACCTACCGCGACAACATCGTCTACGGCATCGACCCGCACGACCGTTCGCACCGCCTGATCATCGCCGAC 
AACACCGTCCACGGGACCAGGAAGAAGCACGGCATCATCGTCTCGCGGGAAGTCAACGACAGCTTCATCTTC 
AACAACCGCAGCTACGAGAACAAGCTTTCCGGGATCGTCCTCGACCGTAACAGCGAGGGCAACCTGGTGGCC 
TACAACGAGGTCTACCGCAACCATTCCGACGGCATCACCCTCTACGAGAGCGGCGACAACCTGCTCTGGGGC 
AACCAGGTCCTGGCCAACCGCCGCCACGGCATCCGCGTGCGCAACAGCGTGAACATCCGCCTCTACGAAAAC 
CTCGCGGCGGGCAACCAGCTGATCGGCGTGTACGGCCACATCAAGGACCTCACCAACACCGACCGCAACATC 
GCCCTCGACCCGTTCGACACCAAGGTCTCGCTGATCGTGGTCGGCGGCAAGCTCGCCGGCAACGGCTCCGGC 
CCGCTCTCGGTGGATTCGCCGCTGAGCCTCGAACTGTACCGGGTGGCGATGCTCGCCCCGACCAAATCCTCC 
GGCATCAGCCTGCCCGGCGTTCTCGGCGAGAAGCAGGACCAGATCCTCGACCTGCTGGTGCGCCAGGACAAG 
GCGGTGCTGATCGATCCCGTGGAAAGCCAGGCCGAACTCCAGGACTGA