ViewGene
Locus Name:
GeneID:
Location:

GeneID:615
TrivialName: PA14_09820
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_09820
PAO1 Ortholog Locus:PA4180
Sequence Length:1644
Protein Length:547
Start: 844784
Stop: 846427
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07567.1|AE004834_7 (AE004834) probable acetolactate synthase large subunit [Pseudomonas aeruginosa PAO1]
Identities = 546/547 (99%), Positives = 546/547 (99%)
Sequence:
ATGGCCAAGGCAGCCGATGTGGTTGTGCAATGCCTGGAAAACGAGGGCGTGCAGTACGTCTTCGGAATTCCC
GGCGAGGAGAATCTCGACCTGCTGGAGTCCCTGCGCAAGTCGCAGATCCGGCTGGTGCTCACCCGCCACGAA
CAATCGGCCGGCTTCATGGCCGCCACCTACGGGCGCCTCACCGGCAAGACCGGGGTCAGCCTGTCGACCCTC
GGCCCGGGCGCCACCAACCTGGTCACCGCCGGCGCCTACGCCTACCTGGGCGGCATGCCGATGCTGATGATC
ACCGGGCAGAAGCCGATCAAGAAGTCCAAGCAGGGCCGCTTCCAGATCCTCGACGTGGTCGGCATGATGCAG
CCGCTGACCAAGTACACCCACCAGTTGGCCTCCGCCGACAACATTCCCTCGCGGGTCCGCGAGGCGTTCCGC
CTGGCCGAGGAAGAGAAGCCCGGCGCGGTGCACCTGGAGCTGCCGGAGGACATCGCCGCCGAGCAGACCGAC
AGCCTGCCCATCCCGCCGAGCCTGAGCCGCCGGCCGCTGGCGGAATACAAGGCGATCTGCGCCGCGGTGGAG
AAGCTGCGCGCCGCGCGCAGCCCGATCCTGGTGATCGGCGCCGGGGCCAACCGCAAGATGACCGCCAAGGTC
CTCAAGCAACTGATCGACAAGACCGGCATTCCCTTCGTCACCACCCAGATGGGCAAGGGCGTGGTCGACGAG
CGTCACCCGCGCTTCCTCGGCAACGCCGCGCTGTCCGCCGGCGACTTCGTGCATCGCGCGGTGGAGGCGGCC
GACCTGATCGTCAACGTCGGCCACGACGTGATCGAGAAGCCGCCGTTCTTCATGGTTCGCGGCGGTACCGAG
GTGATCCACGTGAACTTCCGTTCCGCCGAGGTGGACCCGGTGTATTTCCCGCAGATCGAAGTGGTGGGCGAC
ATCGCCAACGCCATCTGGCAGATCGGCGAGGCGCTGGACGATACCGCGCACTGGGACTTCACCCGCCTGCTG
GCGATCCGCGAGGCCAACGAGGCGCAGGTCGCCGAAGGCGCCGACGACCCGCGCTTCCCGCTCTATCCGCAG
CGCCTGGTGGCCGACGTGCGCCGGGCGTTGCCGTCGCAGGGCATAGTCGCCCTGGACAACGGCATGTACAAG
ATCTGGTTCGCCCGCAACTACAAGGCGCACATGCCCAACACCGTGCTGCTGGACAACGCCCTCGCCACCATG
GGCGCCGGCCTGCCCTCGGCGATGGCCGCGCACCTGGTCTACCCGGACCGGCCGATCGTCGCGGTGTGCGGC
GATGGCGGCTTCATGATGAACAGCCAGGAACTGGAAACCGCGGTGCGGCTGAAGATGAACCTGACGGTGATC
ATCCTGCGCGACAACGGCTACGGGATGATCCGCTGGAAGCAATCGAACATGGGCTTCGCCGACTTCGGGCTG
GACTACGGCAACCCGGACTTCGTGCGCTATGCCGAGGCCTACGGCGCCCACGGCCACCGGGTCGACAGTGCC
GAGGGCTTCCTGCCGTTGCTGGAGCGCTGCCTGGGCACGCCCGGGGTGCATGTGATCGATTGCCCGGTGGAT
TACTCGGAGAACGACCGCATCCTCAACGTCGAGCTGCGCGAACGCGCCCTGGCGGTCTGA
Translation:
MAKAADVVVQCLENEGVQYVFGIPGEENLDLLESLRKSQIRLVLTRHEQSAGFMAATYGRLTGKTGVSLSTL
GPGATNLVTAGAYAYLGGMPMLMITGQKPIKKSKQGRFQILDVVGMMQPLTKYTHQLASADNIPSRVREAFR
LAEEEKPGAVHLELPEDIAAEQTDSLPIPPSLSRRPLAEYKAICAAVEKLRAARSPILVIGAGANRKMTAKV
LKQLIDKTGIPFVTTQMGKGVVDERHPRFLGNAALSAGDFVHRAVEAADLIVNVGHDVIEKPPFFMVRGGTE
VIHVNFRSAEVDPVYFPQIEVVGDIANAIWQIGEALDDTAHWDFTRLLAIREANEAQVAEGADDPRFPLYPQ
RLVADVRRALPSQGIVALDNGMYKIWFARNYKAHMPNTVLLDNALATMGAGLPSAMAAHLVYPDRPIVAVCG
DGGFMMNSQELETAVRLKMNLTVIILRDNGYGMIRWKQSNMGFADFGLDYGNPDFVRYAEAYGAHGHRVDSA
EGFLPLLERCLGTPGVHVIDCPVDYSENDRILNVELRERALAV*
AnnotationID:624GeneID:615
AnnotatorUID: diggins
Modification Date/Time: 2005-03-31 08:08:21
Gene Name:
Confidence Code:3
GeneProduct:putative acetolactate synthase large subunit
Cell Localization:()
Synonyms:PA4180
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0028,COG3962
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68969.1|AE016528_7 (AE016786) acetolactate synthase, catabolic, putative [Pseudomonas
           putida KT2440]
          Length = 547

 Score =  997 bits (2577), Expect = 0.0
 Identities = 485/547 (88%), Positives = 527/547 (96%)

>dbj|BAB53323.1| (AP003011) acetolactate synthase large subunit [Mesorhizobium loti]
          Length = 548

 Score =  604 bits (1558), Expect = e-172
 Identities = 306/544 (56%), Positives = 392/544 (72%), Gaps = 4/544 (0%)

>gb|AAM32036.1| (AE013477) Acetolactate synthase [Methanosarcina mazei Goe1]
          Length = 544

 Score =  495 bits (1274), Expect = e-139
 Identities = 256/542 (47%), Positives = 362/542 (66%), Gaps = 10/542 (1%)






 
Structural Features:
COG0028, IlvB, Thiamine pyrophosphate-requiring enzymes 
[acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, 
phosphonopyruvate decarboxylase] 
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 550 residues,  97.3% aligned
Score =  497 bits (1280), Expect = 2e-141
Identities = 212/540 (39%), Positives = 292/540 (54%), Gaps = 13/540 (2%)

COG3962, COG3962, Acetolactate synthase [Amino acid transport and metabolism]
CD-Length = 617 residues,  87.8% aligned
Score =  175 bits (444), Expect = 2e-44
Identities = 136/553 (24%), Positives = 230/553 (41%), Gaps = 55/553 (9%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1233




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18375




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36910




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:615_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:615
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1045
                           PA4180_tr
                           translation of PA4180
                           547
                           99.81
                           99.81
                           0
                           2813.0
     

     
     
40696
                           gb|AAG07567.1|AE004834_7
                           (AE004834) probable acetolactate synthase large subunit [Pseudomonas aeruginosa PAO1]
                           547
                           99.81
                           99.81
                           0
                           2813.0
     

     
     
40697
                           gb|AAN66782.1|AE016307_1
                           (AE016778) acetolactate synthase, catabolic, putative [Pseudomonas putida KT2440]
                           547
                           88.66
                           96.34
                           0
                           2577.0
     

     
     
40698
                           gb|AAN68969.1|AE016528_7
                           (AE016786) acetolactate synthase, catabolic, putative [Pseudomonas putida KT2440]
                           547
                           88.66
                           96.34
                           0
                           2577.0
     

     
     
40699
                           dbj|BAC49288.1|
                           (AP005949) blr4023 [Bradyrhizobium japonicum USDA 110]
                           556
                           56.29
                           71.76
                           11
                           1586.0
     

     






      

          
GC ORFID: 43617How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4180Glimmer Score: 

          
Start: 4109153Stop: 4107510

          
Length: 1644


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4180 translation of PA4180Homolog Bit Score 1088.0

          
Other Homologs:  t_PA4180 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCAAGGCAGCCGATGTGGTTGTGCAATGCCTGGAAAACGAGGGCGTGCAGTACGTCTTCGGAATTCCC 
GGCGAGGAGAATCTCGACCTGCTGGAGTCCCTGCGCAAGTCGCAGATCCGGCTGGTGCTCACCCGCCACGAA 
CAATCGGCCGGCTTCATGGCCGCCACCTACGGGCGCCTCACCGGCAAGACCGGGGTCAGCCTGTCGACCCTC 
GGCCCGGGCGCCACCAACCTGGTCACCGCCGGCGCCTACGCCTACCTGGGCGGCATGCCGATGCTGATGATC 
ACCGGGCAGAAGCCGATCAAGAAGTCCAAGCAGGGCCGCTTCCAGATCCTCGACGTGGTCGGCATGATGCAG 
CCGCTGACCAAGTACACCCACCAGTTGGCCTCCGCCGACAACATTCCCTCGCGGGTCCGCGAGGCGTTCCGC 
CTGGCCGAGGAAGAGAAGCCCGGCGCGGTGCACCTGGAGCTGCCGGAGGACATCGCCGCCGAGCAGACCGAC 
AGCCTGCCCATCCCGCCGAGCCTGAGCCGCCGGCCGCTGGCGGAATACAAGGCGATCTGCGCCGCGGTGGAG 
AAGCTGCGCGCCGCGCGCAGCCCGATCCTGGTGATCGGCGCCGGGGCCAACCGCAAGATGACCGCCAAGGTC 
CTCAAGCAACTGATCGACAAGACCGGCATTCCCTTCGTCACCACCCAGATGGGCAAGGGCGTGGTCGACGAG 
CGTCACCCGCGCTTCCTCGGCAACGCCGCGCTGTCCGCCGGCGACTTCGTGCATCGCGCGGTGGAGGCGGCC 
GACCTGATCGTCAACGTCGGCCACGACGTGATCGAGAAGCCGCCGTTCTTCATGGTTCGCGGCGGTACCGAG 
GTGATCCACGTGAACTTCCGTTCCGCCGAGGTGGACCCGGTGTATTTCCCGCAGATCGAAGTGGTGGGCGAC 
ATCGCCAACGCCATCTGGCAGATCGGCGAGGCGCTGGACGATACCGCGCACTGGGACTTCACCCGCCTGCTG 
GCGATCCGCGAGGCCAACGAGGCGCAGGTCGCCGAAGGCGCCGACGACCCGCGCTTCCCGCTCTATCCGCAG 
CGCCTGGTGGCCGACGTGCGCCGGGCGTTGCCGTCGCAGGGCATAGTCGCCCTGGACAACGGCATGTACAAG 
ATCTGGTTCGCCCGCAACTACAAGGCGCACATGCCCAACACCGTGCTGCTGGACAACGCCCTCGCCACCATG 
GGCGCCGGCCTGCCCTCGGCGATGGCCGCGCACCTGGTCTACCCGGACCGGCCGATCGTCGCGGTGTGCGGC 
GATGGCGGCTTCATGATGAACAGCCAGGAACTGGAAACCGCGGTGCGGCTGAAGATGAACCTGACGGTGATC 
ATCCTGCGCGACAACGGCTACGGGATGATCCGCTGGAAGCAATCGAACATGGGCTTCGCCGACTTCGGGCTG 
GACTACGGCAACCCGGACTTCGTGCGCTATGCCGAGGCCTACGGCGCCCACGGCCACCGGGTCGACAGTGCC 
GAGGGCTTCCTGCCGTTGCTGGAGCGCTGCCTGGGCACGCCCGGGGTGCATGTGATCGATTGCCCGGTGGAT 
TACTCGGAGAACGACCGCATCCTCAACGTCGAGCTGCGCGAACGCGCCCTGGCGGTCTGA