ViewGene
Locus Name:
GeneID:
Location:

GeneID:611
TrivialName: PA14_02310
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02310
PAO1 Ortholog Locus:PA0183
Sequence Length:1611
Protein Length:536
Start: 208394
Stop: 206784
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03573.1|AE004456_4 (AE004456) arylsulfatase [Pseudomonas aeruginosa PAO1]
Identities = 531/536 (99%), Positives = 534/536 (99%)
Sequence:
ATGAGCAAACGCCCCAACTTCCTGGTGATCGTCGCCGACGACCTGGGCTTCTCCGATATCGGCGCCTTCGGC
GGCGAGATCGCCACGCCGAACCTCGACGCCCTGGCCATCGCCGGCCTGCGCCTGACCGACTTCCACACCGCC
TCGACCTGCTCGCCGACCCGCTCGATGCTGCTCACCGGCACCGACCACCACATCGCCGGGATCGGCACCATG
GCCGAGGCGCTGACCCCGGAACTGGCAGGCAAGCCGGGTTACGAAGGGCATCTCAACGAGCGCGTGGTGGCG
CTGCCGGAGCTGCTCCGCGAGGCCGGCTACCAGACCCTCATGGCCGGCAAGTGGCACCTCGGTCTGAAGCCG
GAACAGACGCCCCATGCACGCGGTTTCGAGCGTTCCTTCTCGCTGCTGCCGGGCGCCGCCAACCACTACGGC
TTCGAGCCTCCCTACGACGAAAGCACTCCGCGCATCCTCAAGGGCACGCCGGCGCTCTACGTGGAAGACGAG
CGCTACCTCGACACGCTGCCGGAGGGTTTCTATTCCTCCGATGCCTTCGGCGACAAGCTGCTGCACTACCTC
AAGGAGCGCGACCAGAGCCGGCCGTTCTTCGCCTACCTGCCGTTCTCCGCGCCGCACTGGCCGCTACAGGCG
CCGCAGGAGATCGTCGAGAAGTACCGCGGCCGCTATGACGCCGGCCCAGAGGCGCTGCGCCAGGAACGCCTG
GCCCGGCTCAAGGAGCTGGGCCTGGTGGAGGCGGACGTCGAAGCCCATCCGGTGCTCGCCCTGAGCCGCGAG
TGGGAAGCCCTGGACGACGAGGAACGGGCGAAGTCGGCGCGGGCGATGGAGGTCTACGCGGCGATGGTCGAG
CGCATGGACTGGAACATCGGCAGGGTCGTGGACTACCTGCGTCGGCAGGGCGAGCTGGACAACACCTTCGTC
CTGTTCATGTCCGACAACGGCGCCGAAGGCGCCCTGCTGGAGGCCTTTCCGAAATTCGGCCCGGATTTGCTG
GGCTTTCTCGACCGGCACTACGACAACAGCCTGGAGAACATCGGCCGCGCCAATTCCTACGTCTGGTATGGC
CCGCGCTGGGCCCAGGCGGCCACCGCGCCGTCGCGCCTGTACAAGGCGTTCACCACCCAGGGCGGCATTCGC
GTGCCAGCGCTGGTGCGCTACCCGCGGCTAAGCCGGCAGGGCGCGATCAGCCATGCCTTCGCCACGGTGATG
GACGTCACCCCGACCCTCCTCGACCTCGCCGGTGTCCGCCACCCAGGCAAGCGCTGGCGCGGCCGCGAGATC
GCCGAACCGCGCGGCAGGTCGTGGCTGGGCTGGCTTTCCGGCGAGACCGAGGCGGCCCACGACGAGAACACC
GTGACCGGCTGGGAGCTGTTCGGCATGCGTGCGATCCGCCAGGGCGACTGGAAGGCGGTGTACCTGCCGGCC
CCGGTGGGCCCGGCCACCTGGCAGCTCTACGACCTGGCCCGCGACCCGGGCGAGATCCACGACCTCGCTGAC
AGCCAGCCGGGCAAGCTGGCGGAGCTGATCGAGCATTGGAAGCGCTACGTCAGCGAGACCGGTGTCGTAGAG
GGGGCTTCGCCTTTCCTGGTGCGATAA
Translation:
MSKRPNFLVIVADDLGFSDIGAFGGEIATPNLDALAIAGLRLTDFHTASTCSPTRSMLLTGTDHHIAGIGTM
AEALTPELAGKPGYEGHLNERVVALPELLREAGYQTLMAGKWHLGLKPEQTPHARGFERSFSLLPGAANHYG
FEPPYDESTPRILKGTPALYVEDERYLDTLPEGFYSSDAFGDKLLHYLKERDQSRPFFAYLPFSAPHWPLQA
PQEIVEKYRGRYDAGPEALRQERLARLKELGLVEADVEAHPVLALSREWEALDDEERAKSARAMEVYAAMVE
RMDWNIGRVVDYLRRQGELDNTFVLFMSDNGAEGALLEAFPKFGPDLLGFLDRHYDNSLENIGRANSYVWYG
PRWAQAATAPSRLYKAFTTQGGIRVPALVRYPRLSRQGAISHAFATVMDVTPTLLDLAGVRHPGKRWRGREI
AEPRGRSWLGWLSGETEAAHDENTVTGWELFGMRAIRQGDWKAVYLPAPVGPATWQLYDLARDPGEIHDLAD
SQPGKLAELIEHWKRYVSETGVVEGASPFLVR*
AnnotationID:620GeneID:611
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 09:09:31
Gene Name:atsA
Confidence Code:1
GeneProduct:arylsulfatase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:hydolysis of arylsulfatase esters
Functional Category Confidence Code:5
COGs:COG3119,pfam00884
Secondary Functional Category(ies):carbon compond metabolism
EC Number:3.1.6.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68956.1|AE016527_2 (AE016786) arylsulfatase, putative [Pseudomonas putida KT2440]
          Length = 554

 Score =  442 bits (1138), Expect = e-123
 Identities = 236/543 (43%), Positives = 328/543 (60%), Gaps = 26/543 (4%)








 
Structural Features:
COG3119, AslA, Arylsulfatase A and related enzymes 
[Inorganic ion transport and metabolism]
CD-Length = 475 residues, 100.0% aligned
Score =  298 bits (765), Expect = 8e-82
Identities = 179/548 (32%), Positives = 238/548 (43%), Gaps = 85/548 (15%)

pfam00884, Sulfatase, Sulfatase.
CD-Length = 329 residues, 100.0% aligned
Score =  165 bits (418), Expect = 1e-41
Identities = 114/508 (22%), Positives = 163/508 (32%), Gaps = 186/508 (36%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1225




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18367




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33552




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:611_cdd.html


    		




 
URL:

















    ReferenceID:35482




  
Author/Investigator(s):
        Beil S, Kehrli H, James P, Staudenmann W, Cook AM, Leisinger T, Kertesz MA.





  
Title:
        Purification and characterization of the arylsulfatase synthesized by Pseudomonas aeruginosa PAO during growth in sulfate-free medium and cloning of the arylsulfatase gene (atsA).




  
PubMed:
        7744061


MedLine:
        




    
Source:
Eur J Biochem. 1995 Apr 15;229(2):385-94.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7744061









           
Homologs By Global Alignment 

           

       



     
Gene ID:611
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1041
                           PA0183_tr
                           translation of PA0183
                           536
                           99.06
                           99.62
                           0
                           2823.0
     

     
     
40525
                           gb|AAG03573.1|AE004456_4
                           (AE004456) arylsulfatase [Pseudomonas aeruginosa PAO1]
                           536
                           99.06
                           99.62
                           0
                           2823.0
     

     
     
40526
                           emb|CAA88421.2|
                           (Z48540) arylsulfatase [Pseudomonas aeruginosa]
                           536
                           99.06
                           99.62
                           0
                           2823.0
     

     
     
40528
                           gb|AAN68956.1|AE016527_2
                           (AE016786) arylsulfatase, putative [Pseudomonas putida KT2440]
                           571
                           41.50
                           57.79
                           52
                           1162.0
     

     
     
40527
                           gb|AAP44252.1|
                           (AY208917) arylsulfatase [Pseudomonas sp. ND6]
                           676
                           35.79
                           50.14
                           157
                           1182.0
     

     






      

          
GC ORFID: 43613How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0183Glimmer Score: 

          
Start: 6009733Stop: 6008123

          
Length: 1611


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0183 translation of PA0183Homolog Bit Score 1092.0

          
Other Homologs:  t_PA0183 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAACGCCCCAACTTCCTGGTGATCGTCGCCGACGACCTGGGCTTCTCCGATATCGGCGCCTTCGGC 
GGCGAGATCGCCACGCCGAACCTCGACGCCCTGGCCATCGCCGGCCTGCGCCTGACCGACTTCCACACCGCC 
TCGACCTGCTCGCCGACCCGCTCGATGCTGCTCACCGGCACCGACCACCACATCGCCGGGATCGGCACCATG 
GCCGAGGCGCTGACCCCGGAACTGGCAGGCAAGCCGGGTTACGAAGGGCATCTCAACGAGCGCGTGGTGGCG 
CTGCCGGAGCTGCTCCGCGAGGCCGGCTACCAGACCCTCATGGCCGGCAAGTGGCACCTCGGTCTGAAGCCG 
GAACAGACGCCCCATGCACGCGGTTTCGAGCGTTCCTTCTCGCTGCTGCCGGGCGCCGCCAACCACTACGGC 
TTCGAGCCTCCCTACGACGAAAGCACTCCGCGCATCCTCAAGGGCACGCCGGCGCTCTACGTGGAAGACGAG 
CGCTACCTCGACACGCTGCCGGAGGGTTTCTATTCCTCCGATGCCTTCGGCGACAAGCTGCTGCACTACCTC 
AAGGAGCGCGACCAGAGCCGGCCGTTCTTCGCCTACCTGCCGTTCTCCGCGCCGCACTGGCCGCTACAGGCG 
CCGCAGGAGATCGTCGAGAAGTACCGCGGCCGCTATGACGCCGGCCCAGAGGCGCTGCGCCAGGAACGCCTG 
GCCCGGCTCAAGGAGCTGGGCCTGGTGGAGGCGGACGTCGAAGCCCATCCGGTGCTCGCCCTGAGCCGCGAG 
TGGGAAGCCCTGGACGACGAGGAACGGGCGAAGTCGGCGCGGGCGATGGAGGTCTACGCGGCGATGGTCGAG 
CGCATGGACTGGAACATCGGCAGGGTCGTGGACTACCTGCGTCGGCAGGGCGAGCTGGACAACACCTTCGTC 
CTGTTCATGTCCGACAACGGCGCCGAAGGCGCCCTGCTGGAGGCCTTTCCGAAATTCGGCCCGGATTTGCTG 
GGCTTTCTCGACCGGCACTACGACAACAGCCTGGAGAACATCGGCCGCGCCAATTCCTACGTCTGGTATGGC 
CCGCGCTGGGCCCAGGCGGCCACCGCGCCGTCGCGCCTGTACAAGGCGTTCACCACCCAGGGCGGCATTCGC 
GTGCCAGCGCTGGTGCGCTACCCGCGGCTAAGCCGGCAGGGCGCGATCAGCCATGCCTTCGCCACGGTGATG 
GACGTCACCCCGACCCTCCTCGACCTCGCCGGTGTCCGCCACCCAGGCAAGCGCTGGCGCGGCCGCGAGATC 
GCCGAACCGCGCGGCAGGTCGTGGCTGGGCTGGCTTTCCGGCGAGACCGAGGCGGCCCACGACGAGAACACC 
GTGACCGGCTGGGAGCTGTTCGGCATGCGTGCGATCCGCCAGGGCGACTGGAAGGCGGTGTACCTGCCGGCC 
CCGGTGGGCCCGGCCACCTGGCAGCTCTACGACCTGGCCCGCGACCCGGGCGAGATCCACGACCTCGCTGAC 
AGCCAGCCGGGCAAGCTGGCGGAGCTGATCGAGCATTGGAAGCGCTACGTCAGCGAGACCGGTGTCGTAGAG 
GGGGCTTCGCCTTTCCTGGTGCGATAA