ViewGene
Locus Name:
GeneID:
Location:

GeneID:61
TrivialName: PA14_18780
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18780
PAO1 Ortholog Locus:PA3522
Sequence Length:3162
Protein Length:1053
Start: 1615483
Stop: 1618644
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06910.1|AE004772_8 (AE004772) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
Identities = 1049/1053 (99%), Positives = 1052/1053 (99%)
Sequence:
ATGACATTCCCACGCTTCTTCATCGATAGGCCGATCTTCGCCATCGTTCTTTCGGTGCTGATGATGATCGGA
GGCATCGTTTCGTTCTTCCAGTTGCCGCTGAGCGAATACCCGGCGGTGACGCCGCCGACCGTGCAGGTGACC
ACCGCCTATCCCGGCGCCAACCCCGACGTGATCGCGCAGACGGTGGCGACGCCGCTGGAGCAGGCGATCACC
GGGGTCGAGGGGATGCTGTACATGTCCTCGCAGTCGGCCACCGACGGGCGAATGATCCTGACCATCACCTTC
GACCAGCACATCGATCCCGACATGGCCCAGATCCAGGTGCAGAACCGGGTCTCGCGGGTGCTCTCGCGCCTG
CCCGACGAGGTGCAGCGGCAGGGCGTGGTCACCCAGAAGACCTCGCCGGACATCCTCATGGTGGTGCACCTG
CTGTCGCCGGAACAACGCTACGACCCGCTGTACATCTCGAACTACGCCTACCTCCAGGTACGCGACGAACTG
CTGCGCCTGCCGGGGATCAGCGATGTGGTGGTGTGGGGCGCCGGCGAATACAGCATGCGCCTGTGGCTCGAC
CCCGACCTGATCGCCGCGCGCGGGCTGACGGCGGGCGAGGTGATCGCCGCGGTTCGCGAGCAGAACGTCCAG
GTGGCGGCCGGCGCCGTCGGCCAGGCGCCGGACTCCACCGCGGCCTTCCAGGTCACGGTGAATACCCTCGGG
CGCCTGAGCGACGAAGAGCAGTTCGGCGACATCATCGTCCGCACCGGCGCCGACGGCCAGGTGACGCGCCTG
CGCGACGTCGCGCGGATCGAGATGGGCGGCGATGCCTATGCGCTGCGCAGCCTGCTGGACGGCGAGCCGGCG
GTGGCCTTGCAGATCGTCCAGAGCCCCGGAGCCAACGCGCTGGATACCGCCGAGGCGGTGCGTGCCACCGTG
GCGAGGCTCGAAGGCAACTTCCCGGCCGGCCTGAGCGCGCGCATCGCCTACGACCCGACGGTGTTCGTCCGC
GCTTCGTTGCAGACCGTGGCCACCACCCTGCTGGAGGCGATCCTGCTGGTCGTGGTGGTGGTGGTGCTGTTC
CTGCGTAGCTGGCGTGCTTCGCTGATTCCGCTGATGGCCGTGCCGGTCTCGCTGGTCGGCACCTTCGCCATC
ATGCATCTGATGGGTTTCTCGCTGAATACCCTGTCGCTGTTCGGCCTGGTGCTGTCGATCGGCATCGTCGTC
GATGACGCCATCGTGGTGGTGGAAAACGTCGAGCGCCACATCGAGAACGGCGAGCCGCCGCTCCAGGCCGCG
CGGCGTGCGATGGACGAGGTCACCGGACCGATCATGGCGATTACCTCGGTGCTGGCGGCAGTGTTCATCCCC
ACGGCCTTTCTCAGTGGTCTGCAGGGCGAGTTCTATCGTCAGTTCGCCCTGACCATCGCCATCTCCACCATC
CTGTCGGCGCTCAACTCGCTGACCCTCAGCCCGGCCCTGGCGGGCCTGCTGTTGCGTCCGCGGCCGGCCGGA
GATGCGGTGGCGGGGCGCTTCCAGCGACTGCTCCAGGTCCTCGGCCGACCGTTGCGAAATGCCCCCGAGGCC
TACGGCAACGCGGTGCGCAAGGTGGTGCGGGTGAGCGGCCTGGCCCTGGTGGTGTATGGGGGCCTGCTTGGC
CTGACCTGGGTCGGTTTCCAGGCGGTGCCGCCGGGCTTCGTGCCGATGCAGGACAAGTACTATCTGGTCGGC
ATCGCCCAGTTGCCGAACGGAGCGTCGCTGGAGCGTACCGAAGCGGTGGTCAGGCAGATGTCCCGGATCGGC
CTCGACGAGCCGGGGGTGGAGAGCGTCGTGGCCTTCCCGGGCCTGTCGGTGAACGGCTTCGTCAACGTGCCG
AACGCCGCGGTCATGTTCTTCATGCTCGACCCGTTCGAATCGCGCACCTCCGCCGATCTCGGCGCGCTGGCC
ATCGCCGGGCGCCTGCAAGCCAGGTTCGCCAGCATTCCCGACGGCTTCCTCGGGGTCTTCCCGCCGCCGCCG
GTACCGGGGCTGGGAACCATCGGCGGCTTCAAGATGCAGGTCGAGGATCGCGGCGGCGCCGGGCTGGAAGCC
CTGGCCAGGCAGACCCAGGTGCTGATGATGAAGGCCACTGAGTCCGGCCAGCTCGGGGGCTTGATGACCAGC
TTCGATATCAATGCCCCGCAGCTCGAGGTGGTGGTCGACCGCACCAAGGTGAAGAGCCAGGGCGTTCGCCTG
GCCGATGTGTTCGAGGCATTGCAGGTCTACCTCGGCTCGCTGTACATCAACGACTTCAACCGCTTCGGGCGC
ACCTACAAGGTCACTGCCCAGGCCGATGCGCCGCACCGCATGCAGGCCGAAGCCATCGGGCGCCTGCAGGTA
CGCAATGCCGCCGGGGCGATGTTGCCGCTGTCCTCGTTCGTCACCGTGACGCCCAGCTCCGGTCCCGACCGG
GTGATCCACTACAACGGCTATCCGTCGGCCGATATCAGCGGCGGCGCGCTTCCCGGCGTCAGTTCGGGACAG
GCGGTGGCGTTGATGGAGCGCCTGGCCGGCGAAGTGCTGCCCGAGGGCATGACCTTCGAATGGACCGACCTG
ACCTACCAGCAGAAGCTGGCCGGTAACAGCGCGCTGTTCATCTTCCCGCTGTGCGTGCTGCTGGCCTACCTG
ATCCTCGCCGCGCAGTACAACAGCTGGCTGTTGCCGCTGGCGGTCCTGCTGATCGTGCCGATGTGCCTGCTC
AGCGCGATCGCCGGGGTGTGGCTGGTGGGCGGCGACAACAACGTGTTCGTCCAGATCGGGCTGGTGGTGCTG
GTCGGCCTGGCGGCGAAGAACGCGATCCTGATCGTCGAGTTCGCCCGCACGCTGGAGGCCGAGGGCGCCCGT
GCGCTGGAGGCGGTGGTCGAGGCTTGCCGCCTGCGCCTGCGGCCGATCCTGATGACCTCGCTGGCGTTCATC
GCCGGCGTGGTGCCGTTGGTCATGGCCAGCGGCGCCGGAGCGGAAATGCGCCAGGCCATGGGCGTCGCGGTA
TTCGCCGGGATGCTCGGGGTGACGCTGTTCGGCCTGTTCCTCACGCCAGTGTTCTACGTACTGGTACGGGCA
CTGGCGGCGCGCCTGGAGCGACGCGCCGGCGGCAGCCTGGCGCATCTGGAGGGAGGCCGTCCATGA
Translation:
MTFPRFFIDRPIFAIVLSVLMMIGGIVSFFQLPLSEYPAVTPPTVQVTTAYPGANPDVIAQTVATPLEQAIT
GVEGMLYMSSQSATDGRMILTITFDQHIDPDMAQIQVQNRVSRVLSRLPDEVQRQGVVTQKTSPDILMVVHL
LSPEQRYDPLYISNYAYLQVRDELLRLPGISDVVVWGAGEYSMRLWLDPDLIAARGLTAGEVIAAVREQNVQ
VAAGAVGQAPDSTAAFQVTVNTLGRLSDEEQFGDIIVRTGADGQVTRLRDVARIEMGGDAYALRSLLDGEPA
VALQIVQSPGANALDTAEAVRATVARLEGNFPAGLSARIAYDPTVFVRASLQTVATTLLEAILLVVVVVVLF
LRSWRASLIPLMAVPVSLVGTFAIMHLMGFSLNTLSLFGLVLSIGIVVDDAIVVVENVERHIENGEPPLQAA
RRAMDEVTGPIMAITSVLAAVFIPTAFLSGLQGEFYRQFALTIAISTILSALNSLTLSPALAGLLLRPRPAG
DAVAGRFQRLLQVLGRPLRNAPEAYGNAVRKVVRVSGLALVVYGGLLGLTWVGFQAVPPGFVPMQDKYYLVG
IAQLPNGASLERTEAVVRQMSRIGLDEPGVESVVAFPGLSVNGFVNVPNAAVMFFMLDPFESRTSADLGALA
IAGRLQARFASIPDGFLGVFPPPPVPGLGTIGGFKMQVEDRGGAGLEALARQTQVLMMKATESGQLGGLMTS
FDINAPQLEVVVDRTKVKSQGVRLADVFEALQVYLGSLYINDFNRFGRTYKVTAQADAPHRMQAEAIGRLQV
RNAAGAMLPLSSFVTVTPSSGPDRVIHYNGYPSADISGGALPGVSSGQAVALMERLAGEVLPEGMTFEWTDL
TYQQKLAGNSALFIFPLCVLLAYLILAAQYNSWLLPLAVLLIVPMCLLSAIAGVWLVGGDNNVFVQIGLVVL
VGLAAKNAILIVEFARTLEAEGARALEAVVEACRLRLRPILMTSLAFIAGVVPLVMASGAGAEMRQAMGVAV
FAGMLGVTLFGLFLTPVFYVLVRALAARLERRAGGSLAHLEGGRP*
AnnotationID:71GeneID:61
AnnotatorUID: diggins
Modification Date/Time: 2005-04-19 15:03:53
Gene Name:
Confidence Code:3
GeneProduct:putative RND efflux transporter
Cell Localization:()
Synonyms:mexF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00873,COG0841,COG3696
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56587.1| (AE016867) multidrug efflux RND transporter MexF [Pseudomonas
            syringae pv. tomato str. DC3000]
          Length = 1063

 Score = 1280 bits (3311), Expect = 0.0
 Identities = 645/1059 (60%), Positives = 812/1059 (76%), Gaps = 13/1059 (1%)

>gb|AAN56485.1|AE015785_8 (AE015785) RND multidrug efflux transporter MexF [Shewanella
            oneidensis MR-1]
          Length = 1047

 Score = 1227 bits (3174), Expect = 0.0
 Identities = 622/1029 (60%), Positives = 786/1029 (76%), Gaps = 6/1029 (0%)

>gb|AAM40737.1| (AE012244) RND multidrug efflux transporter MexF [Xanthomonas
            campestris pv. campestris str. ATCC 33913]
          Length = 1056

 Score = 1144 bits (2958), Expect = 0.0
 Identities = 603/1059 (56%), Positives = 765/1059 (72%), Gaps = 20/1059 (1%)








 
Structural Features:
pfam00873, ACR_tran, AcrB/AcrD/AcrF family.
CD-Length = 1017 residues, 100.0% aligned
Score =  984 bits (2546), Expect = 0.0
Identities = 442/1032 (42%), Positives = 645/1032 (62%), Gaps = 18/1032 (1%)

COG0841, AcrB, Cation/multidrug efflux pump [Defense 
mechanisms]
CD-Length = 1009 residues,  99.9% aligned
Score =  984 bits (2546), Expect = 0.0
Identities = 496/1034 (47%), Positives = 670/1034 (64%), Gaps = 29/1034 (2%)

COG3696, Putative silver efflux pump 
[Inorganic ion transport and metabolism]
CD-Length = 1027 residues,  98.5% aligned
Score =  413 bits (1062), Expect = 7e-116
Identities = 256/1043 (24%), Positives = 466/1043 (44%), Gaps = 48/1043 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:125




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17267




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32044




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:61_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:61
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
117
                           PA3522_tr
                           translation of PA3522
                           1053
                           99.62
                           99.90
                           0
                           5218.0
     

     
     
12674
                           gb|AAG06910.1|AE004772_8
                           (AE004772) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
                           1053
                           99.62
                           99.90
                           0
                           5218.0
     

     
     
12675
                           gb|AAL19305.1|
                           (AE008711) putative cation efflux system protein [Salmonella typhimurium LT2]
                           1064
                           68.70
                           80.92
                           20
                           3714.0
     

     
     
12677
                           gb|AAN69028.1|AE016535_2
                           (AE016786) multidrug efflux RND transporter MexF [Pseudomonas putida KT2440]
                           1064
                           60.71
                           76.59
                           16
                           3306.0
     

     
     
12676
                           gb|AAO56587.1|
                           (AE016867) multidrug efflux RND transporter MexF [Pseudomonas syringae pv. tomato str. DC3000]
                           1067
                           60.63
                           76.28
                           18
                           3320.0
     

     






      

          
GC ORFID: 43063How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3522Glimmer Score: 

          
Start: 3338451Stop: 3335290

          
Length: 3162


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3522 translation of PA3522Homolog Bit Score 2014.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7199 (ORF: BLASTX 1275.0), t_PA2494 (ORF: BLASTX 1248.0), t_PA4598 (ORF: BLASTX 762.0), t_PA2018 (ORF: BLASTX 752.0), psyr_15may02_Scaffold7_revised_gene7337 (ORF: BLASTX 109.0), t_PA3522 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACATTCCCACGCTTCTTCATCGATAGGCCGATCTTCGCCATCGTTCTTTCGGTGCTGATGATGATCGGA 
GGCATCGTTTCGTTCTTCCAGTTGCCGCTGAGCGAATACCCGGCGGTGACGCCGCCGACCGTGCAGGTGACC 
ACCGCCTATCCCGGCGCCAACCCCGACGTGATCGCGCAGACGGTGGCGACGCCGCTGGAGCAGGCGATCACC 
GGGGTCGAGGGGATGCTGTACATGTCCTCGCAGTCGGCCACCGACGGGCGAATGATCCTGACCATCACCTTC 
GACCAGCACATCGATCCCGACATGGCCCAGATCCAGGTGCAGAACCGGGTCTCGCGGGTGCTCTCGCGCCTG 
CCCGACGAGGTGCAGCGGCAGGGCGTGGTCACCCAGAAGACCTCGCCGGACATCCTCATGGTGGTGCACCTG 
CTGTCGCCGGAACAACGCTACGACCCGCTGTACATCTCGAACTACGCCTACCTCCAGGTACGCGACGAACTG 
CTGCGCCTGCCGGGGATCAGCGATGTGGTGGTGTGGGGCGCCGGCGAATACAGCATGCGCCTGTGGCTCGAC 
CCCGACCTGATCGCCGCGCGCGGGCTGACGGCGGGCGAGGTGATCGCCGCGGTTCGCGAGCAGAACGTCCAG 
GTGGCGGCCGGCGCCGTCGGCCAGGCGCCGGACTCCACCGCGGCCTTCCAGGTCACGGTGAATACCCTCGGG 
CGCCTGAGCGACGAAGAGCAGTTCGGCGACATCATCGTCCGCACCGGCGCCGACGGCCAGGTGACGCGCCTG 
CGCGACGTCGCGCGGATCGAGATGGGCGGCGATGCCTATGCGCTGCGCAGCCTGCTGGACGGCGAGCCGGCG 
GTGGCCTTGCAGATCGTCCAGAGCCCCGGAGCCAACGCGCTGGATACCGCCGAGGCGGTGCGTGCCACCGTG 
GCGAGGCTCGAAGGCAACTTCCCGGCCGGCCTGAGCGCGCGCATCGCCTACGACCCGACGGTGTTCGTCCGC 
GCTTCGTTGCAGACCGTGGCCACCACCCTGCTGGAGGCGATCCTGCTGGTCGTGGTGGTGGTGGTGCTGTTC 
CTGCGTAGCTGGCGTGCTTCGCTGATTCCGCTGATGGCCGTGCCGGTCTCGCTGGTCGGCACCTTCGCCATC 
ATGCATCTGATGGGTTTCTCGCTGAATACCCTGTCGCTGTTCGGCCTGGTGCTGTCGATCGGCATCGTCGTC 
GATGACGCCATCGTGGTGGTGGAAAACGTCGAGCGCCACATCGAGAACGGCGAGCCGCCGCTCCAGGCCGCG 
CGGCGTGCGATGGACGAGGTCACCGGACCGATCATGGCGATTACCTCGGTGCTGGCGGCAGTGTTCATCCCC 
ACGGCCTTTCTCAGTGGTCTGCAGGGCGAGTTCTATCGTCAGTTCGCCCTGACCATCGCCATCTCCACCATC 
CTGTCGGCGCTCAACTCGCTGACCCTCAGCCCGGCCCTGGCGGGCCTGCTGTTGCGTCCGCGGCCGGCCGGA 
GATGCGGTGGCGGGGCGCTTCCAGCGACTGCTCCAGGTCCTCGGCCGACCGTTGCGAAATGCCCCCGAGGCC 
TACGGCAACGCGGTGCGCAAGGTGGTGCGGGTGAGCGGCCTGGCCCTGGTGGTGTATGGGGGCCTGCTTGGC 
CTGACCTGGGTCGGTTTCCAGGCGGTGCCGCCGGGCTTCGTGCCGATGCAGGACAAGTACTATCTGGTCGGC 
ATCGCCCAGTTGCCGAACGGAGCGTCGCTGGAGCGTACCGAAGCGGTGGTCAGGCAGATGTCCCGGATCGGC 
CTCGACGAGCCGGGGGTGGAGAGCGTCGTGGCCTTCCCGGGCCTGTCGGTGAACGGCTTCGTCAACGTGCCG 
AACGCCGCGGTCATGTTCTTCATGCTCGACCCGTTCGAATCGCGCACCTCCGCCGATCTCGGCGCGCTGGCC 
ATCGCCGGGCGCCTGCAAGCCAGGTTCGCCAGCATTCCCGACGGCTTCCTCGGGGTCTTCCCGCCGCCGCCG 
GTACCGGGGCTGGGAACCATCGGCGGCTTCAAGATGCAGGTCGAGGATCGCGGCGGCGCCGGGCTGGAAGCC 
CTGGCCAGGCAGACCCAGGTGCTGATGATGAAGGCCACTGAGTCCGGCCAGCTCGGGGGCTTGATGACCAGC 
TTCGATATCAATGCCCCGCAGCTCGAGGTGGTGGTCGACCGCACCAAGGTGAAGAGCCAGGGCGTTCGCCTG 
GCCGATGTGTTCGAGGCATTGCAGGTCTACCTCGGCTCGCTGTACATCAACGACTTCAACCGCTTCGGGCGC 
ACCTACAAGGTCACTGCCCAGGCCGATGCGCCGCACCGCATGCAGGCCGAAGCCATCGGGCGCCTGCAGGTA 
CGCAATGCCGCCGGGGCGATGTTGCCGCTGTCCTCGTTCGTCACCGTGACGCCCAGCTCCGGTCCCGACCGG 
GTGATCCACTACAACGGCTATCCGTCGGCCGATATCAGCGGCGGCGCGCTTCCCGGCGTCAGTTCGGGACAG 
GCGGTGGCGTTGATGGAGCGCCTGGCCGGCGAAGTGCTGCCCGAGGGCATGACCTTCGAATGGACCGACCTG 
ACCTACCAGCAGAAGCTGGCCGGTAACAGCGCGCTGTTCATCTTCCCGCTGTGCGTGCTGCTGGCCTACCTG 
ATCCTCGCCGCGCAGTACAACAGCTGGCTGTTGCCGCTGGCGGTCCTGCTGATCGTGCCGATGTGCCTGCTC 
AGCGCGATCGCCGGGGTGTGGCTGGTGGGCGGCGACAACAACGTGTTCGTCCAGATCGGGCTGGTGGTGCTG 
GTCGGCCTGGCGGCGAAGAACGCGATCCTGATCGTCGAGTTCGCCCGCACGCTGGAGGCCGAGGGCGCCCGT 
GCGCTGGAGGCGGTGGTCGAGGCTTGCCGCCTGCGCCTGCGGCCGATCCTGATGACCTCGCTGGCGTTCATC 
GCCGGCGTGGTGCCGTTGGTCATGGCCAGCGGCGCCGGAGCGGAAATGCGCCAGGCCATGGGCGTCGCGGTA 
TTCGCCGGGATGCTCGGGGTGACGCTGTTCGGCCTGTTCCTCACGCCAGTGTTCTACGTACTGGTACGGGCA 
CTGGCGGCGCGCCTGGAGCGACGCGCCGGCGGCAGCCTGGCGCATCTGGAGGGAGGCCGTCCATGA