ViewGene
Locus Name:
GeneID:
Location:

GeneID:606
TrivialName: PA14_09240
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_09240
PAO1 Ortholog Locus:PA4228
Sequence Length:1644
Protein Length:547
Start: 784394
Stop: 782751
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAD55799.1|AF184620_1 (AF184620) salicyl-AMP ligase PchD [Pseudomonas aeruginosa]
Identities = 546/547 (99%), Positives = 546/547 (99%)
Sequence:
ATGACTTCCTCGCCCGCCACCCCATCGGCCGTCGACGACGCCCCCGACTGGCCCGCCGCCTTCGTCCGCCGC
TATCTCGACGCCGGCCACTGGCAGGACCAGAACTTCGCCGAAGCCCTGGCCGCCAGCGCCGCGCGCCATCCG
CGGCGGATCGCACTCTGCGACGACGACCAGCGGCTCAGCTATGCCGACCTGCTCCAGCGCTGCCGCCGCCTG
GCAGCCGGACTGCGCCAGGCCGGCCTCGCACATGGCGACACGGTGGTGCTGCACCTGCCCAACGGCATCGCC
TTCGTCGAGACCTGCTTCGCCCTGTTCCAGCTCGGCGTACGCCCGGTGCTCGCCCTCCCCGCCCATCGCCAG
CATGAGATCAGCGGTTTCTGCCGGTTCGCCGAGGCCAAGGCCTACATCGGCGCCGAACGGATCGACGGCTTC
GACCCACGGCCGATGGCCCGTGAGCTGCTCGCCAGCGGCGCCTGCCGGATGGCGCTGATCCACGGCGAGGCC
GAGGCGCCGCTGCAAGCGCTGGCACCGCTGTACCAGGCCGACGCGCTGGAAGACTGCGCCGCCCGCGCCGAG
GATATCGCCTGCTTCCAGTTGTCCGGCGGCACCACCGGCACGCCGAAGCTGATTCCCCGGCGTCATCGCGAG
TATCTCTACAACGTCCGCGCCAGCGCCGAGGTCTGCGGCTTCGACGAGCACACGGTGTACCTCACCGGCTTG
CCGATGGCGCACAACTTCACCCTCTGCTGCCCCGGAGTGATCGGCACGCTGCTGGCCGGCGGCCGCGTGGTG
GTCAGCCAGCGCGCCGATCCCGAGCATTGCTTCGCCCTGATCGCCCGTGAACGGGTCACCCACACCGCCCTG
GTACCGCCGCTGGCGATGCTCTGGCTGGATGCCCAGGAAAGCCGCCGCGCCGATCTATCCAGCCTGCGTCTG
CTCCAGGTCGGCGGTTCCAGGCTCGGCAGCAGTGCCGCGCAACGCGTCGAACCGGTGCTCGGCTGCCAGTTG
CAACAGGTGCTGGGAATGGCCGAGGGGCTGATCTGCTACACCCGCCTGGACGATCCGCCCGAGCGCGTATTG
CATACCCAGGGCCGGCCGCTGTCGCCGGACGACGAAGTGCGGGTGGTCGACGCCGAGGGCCGCGAGGTCGGC
CCCGGCGAGGTCGGCGAACTGACCGTGCGCGGTCCCTACACCATCCGCGGCTACTACCGCCTGCCCGAACAC
AACGCCAAGGCCTTCAGCGCGGACGGCTTCTACCGCACCGGCGACCGGGTCAGCCGCGACAAGGACGGCTAC
CTGGTGGTGGAGGGCCGCGACAAGGACCAGATCAACCGCGGCGGCGAGAAGATCGCTGCGGAAGAGGTGGAG
AACCTGCTGATCGCCCATCCGCAGGTGCACGACGCCACCGTCGTGGCGATGCCCGACAGCCTGCTCGGCGAG
CGCACCTGTGCCTTCGTCATCCCGCGCCAGCCGGCACCCTCGGCGCTGAAGCTGAAGCAATACCTGCACGCC
TGCGGGCTGGCCGCGTTCAAGGTGCCGGACCGCATCGAGCTGGTCCCGGCCTTCCCCCAGACCGGCATCGGC
AAGATCAGCAAGAAGGACCTGCGCGAACGCCTGCGCCGCGAGCTGGAGGCCCGCGCATGA
Translation:
MTSSPATPSAVDDAPDWPAAFVRRYLDAGHWQDQNFAEALAASAARHPRRIALCDDDQRLSYADLLQRCRRL
AAGLRQAGLAHGDTVVLHLPNGIAFVETCFALFQLGVRPVLALPAHRQHEISGFCRFAEAKAYIGAERIDGF
DPRPMARELLASGACRMALIHGEAEAPLQALAPLYQADALEDCAARAEDIACFQLSGGTTGTPKLIPRRHRE
YLYNVRASAEVCGFDEHTVYLTGLPMAHNFTLCCPGVIGTLLAGGRVVVSQRADPEHCFALIARERVTHTAL
VPPLAMLWLDAQESRRADLSSLRLLQVGGSRLGSSAAQRVEPVLGCQLQQVLGMAEGLICYTRLDDPPERVL
HTQGRPLSPDDEVRVVDAEGREVGPGEVGELTVRGPYTIRGYYRLPEHNAKAFSADGFYRTGDRVSRDKDGY
LVVEGRDKDQINRGGEKIAAEEVENLLIAHPQVHDATVVAMPDSLLGERTCAFVIPRQPAPSALKLKQYLHA
CGLAAFKVPDRIELVPAFPQTGIGKISKKDLRERLRRELEARA*
AnnotationID:615GeneID:606
AnnotatorUID: diggins
Modification Date/Time: 2005-04-01 07:07:20
Gene Name:pchD
Confidence Code:1
GeneProduct:pyochelin biosynthesis protein PchD
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:salicyl-AMP ligase PchD
Functional Category Confidence Code:5
COGs:COG1021,COG0318,pfam00501
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN15214.1| (AY138812) DHB-AMP-ligase DhbE [Bacillus subtilis]
          Length = 539

 Score =  564 bits (1454), Expect = e-160
 Identities = 291/532 (54%), Positives = 367/532 (68%), Gaps = 7/532 (1%)

>gb|AAF93937.1| (AE004162) vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase
           [Vibrio cholerae]
          Length = 543

 Score =  519 bits (1336), Expect = e-146
 Identities = 268/532 (50%), Positives = 351/532 (65%), Gaps = 8/532 (1%)

>gb|AAP16028.1| (AE016979) 2,3-dihydroxybenzoate-AMP ligase [Shigella flexneri 2a
           str. 2457T]
          Length = 536

 Score =  474 bits (1219), Expect = e-133
 Identities = 246/532 (46%), Positives = 342/532 (64%), Gaps = 6/532 (1%)






 
Structural Features:
COG1021, EntE, Peptide arylation enzymes 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 542 residues, 100.0% aligned
Score =  832 bits (2150), Expect = 0.0
Identities = 333/542 (61%), Positives = 389/542 (71%), Gaps = 3/542 (0%)

COG0318, CaiC, Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II 
[Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 534 residues,  98.7% aligned
Score =  329 bits (845), Expect = 5e-91
Identities = 183/532 (34%), Positives = 245/532 (46%), Gaps = 19/532 (3%)

pfam00501, AMP-binding, AMP-binding enzyme.
CD-Length = 412 residues, 100.0% aligned
Score =  289 bits (740), Expect = 6e-79
Identities = 146/416 (35%), Positives = 199/416 (47%), Gaps = 10/416 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1215




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18357




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36927




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:606_cdd.html


    		




 
URL:

















    ReferenceID:37015




  
Author/Investigator(s):
        Serino L, Reimmann C, Visca P, Beyeler M, Chiesa VD, Haas D.





  
Title:
        Biosynthesis of pyochelin and dihydroaeruginoic acid requires the iron-regulated pchDCBA operon in Pseudomonas aeruginosa.




  
PubMed:
        8982005


MedLine:
        




    
Source:
J Bacteriol. 1997 Jan;179(1):248-57.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8982005









           
Homologs By Global Alignment 

           

       



     
Gene ID:606
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
40221
                           gb|AAD55799.1|AF184620_1
                           (AF184620) salicyl-AMP ligase PchD [Pseudomonas aeruginosa]
                           547
                           99.81
                           99.81
                           0
                           2847.0
     

     
     
1034
                           PA4228_tr
                           translation of PA4228
                           547
                           99.26
                           99.45
                           0
                           2832.0
     

     
     
40222
                           gb|AAG07616.1|AE004840_2
                           (AE004840) pyochelin biosynthesis protein PchD [Pseudomonas aeruginosa PAO1]
                           547
                           99.26
                           99.45
                           0
                           2832.0
     

     
     
40223
                           emb|CAA57966.1|
                           (X82644) pchD [Pseudomonas aeruginosa]
                           547
                           98.72
                           98.90
                           1
                           2803.0
     

     
     
40224
                           gb|AAG31128.1|AF299336_5
                           (AF299336) MxcE [Stigmatella aurantiaca]
                           550
                           55.27
                           68.18
                           10
                           1528.0
     

     






      

          
GC ORFID: 43608How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4228Glimmer Score: 

          
Start: 4169543Stop: 4171186

          
Length: 1644


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4228 translation of PA4228Homolog Bit Score 1095.0

          
Other Homologs:  t_PA4228 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTTCCTCGCCCGCCACCCCATCGGCCGTCGACGACGCCCCCGACTGGCCCGCCGCCTTCGTCCGCCGC 
TATCTCGACGCCGGCCACTGGCAGGACCAGAACTTCGCCGAAGCCCTGGCCGCCAGCGCCGCGCGCCATCCG 
CGGCGGATCGCACTCTGCGACGACGACCAGCGGCTCAGCTATGCCGACCTGCTCCAGCGCTGCCGCCGCCTG 
GCAGCCGGACTGCGCCAGGCCGGCCTCGCACATGGCGACACGGTGGTGCTGCACCTGCCCAACGGCATCGCC 
TTCGTCGAGACCTGCTTCGCCCTGTTCCAGCTCGGCGTACGCCCGGTGCTCGCCCTCCCCGCCCATCGCCAG 
CATGAGATCAGCGGTTTCTGCCGGTTCGCCGAGGCCAAGGCCTACATCGGCGCCGAACGGATCGACGGCTTC 
GACCCACGGCCGATGGCCCGTGAGCTGCTCGCCAGCGGCGCCTGCCGGATGGCGCTGATCCACGGCGAGGCC 
GAGGCGCCGCTGCAAGCGCTGGCACCGCTGTACCAGGCCGACGCGCTGGAAGACTGCGCCGCCCGCGCCGAG 
GATATCGCCTGCTTCCAGTTGTCCGGCGGCACCACCGGCACGCCGAAGCTGATTCCCCGGCGTCATCGCGAG 
TATCTCTACAACGTCCGCGCCAGCGCCGAGGTCTGCGGCTTCGACGAGCACACGGTGTACCTCACCGGCTTG 
CCGATGGCGCACAACTTCACCCTCTGCTGCCCCGGAGTGATCGGCACGCTGCTGGCCGGCGGCCGCGTGGTG 
GTCAGCCAGCGCGCCGATCCCGAGCATTGCTTCGCCCTGATCGCCCGTGAACGGGTCACCCACACCGCCCTG 
GTACCGCCGCTGGCGATGCTCTGGCTGGATGCCCAGGAAAGCCGCCGCGCCGATCTATCCAGCCTGCGTCTG 
CTCCAGGTCGGCGGTTCCAGGCTCGGCAGCAGTGCCGCGCAACGCGTCGAACCGGTGCTCGGCTGCCAGTTG 
CAACAGGTGCTGGGAATGGCCGAGGGGCTGATCTGCTACACCCGCCTGGACGATCCGCCCGAGCGCGTATTG 
CATACCCAGGGCCGGCCGCTGTCGCCGGACGACGAAGTGCGGGTGGTCGACGCCGAGGGCCGCGAGGTCGGC 
CCCGGCGAGGTCGGCGAACTGACCGTGCGCGGTCCCTACACCATCCGCGGCTACTACCGCCTGCCCGAACAC 
AACGCCAAGGCCTTCAGCGCGGACGGCTTCTACCGCACCGGCGACCGGGTCAGCCGCGACAAGGACGGCTAC 
CTGGTGGTGGAGGGCCGCGACAAGGACCAGATCAACCGCGGCGGCGAGAAGATCGCTGCGGAAGAGGTGGAG 
AACCTGCTGATCGCCCATCCGCAGGTGCACGACGCCACCGTCGTGGCGATGCCCGACAGCCTGCTCGGCGAG 
CGCACCTGTGCCTTCGTCATCCCGCGCCAGCCGGCACCCTCGGCGCTGAAGCTGAAGCAATACCTGCACGCC 
TGCGGGCTGGCCGCGTTCAAGGTGCCGGACCGCATCGAGCTGGTCCCGGCCTTCCCCCAGACCGGCATCGGC 
AAGATCAGCAAGAAGGACCTGCGCGAACGCCTGCGCCGCGAGCTGGAGGCCCGCGCATGA