ViewGene
Locus Name:
GeneID:
Location:

GeneID:60
TrivialName: PA14_05540
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05540
PAO1 Ortholog Locus:PA0426
Sequence Length:3141
Protein Length:1046
Start: 487942
Stop: 491082
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03815.1|AE004479_10 (AE004479) RND multidrug efflux transporter MexB [Pseudomonas aeruginosa PAO1]
Identities = 1046/1046 (100%), Positives = 1046/1046 (100%)
Sequence:
ATGTCGAAGTTTTTCATTGATAGGCCCATTTTCGCGTGGGTGATCGCCTTGGTGATCATGCTCGCGGGCGGC
CTGTCGATCCTCAGTCTGCCGGTCAACCAGTACCCGGCCATCGCCCCGCCGGCCATCGCCGTGCAGGTGAGC
TACCCGGGCGCCTCGGCCGAGACGGTGCAGGACACCGTGGTCCAGGTGATCGAGCAGCAGATGAACGGGATC
GACAATCTGCGCTACATCTCCTCGGAGAGTAACTCCGACGGCAGCATGACCATCACCGTGACCTTCGAACAG
GGCACCGACCCCGACATCGCCCAGGTCCAGGTGCAGAACAAGCTGCAACTGGCCACCCCGCTGCTGCCGCAG
GAAGTGCAGCGCCAGGGGATCCGGGTGACCAAGGCGGTGAAGAACTTCCTCATGGTGGTCGGTGTGGTTTCC
ACCGACGGCAGCATGACCAAGGAAGACCTGTCGAACTACATCGTTTCCAACATCCAGGACCCGCTCTCGCGG
ACCAAGGGCGTCGGTGACTTCCAGGTGTTCGGCTCGCAGTACTCGATGCGCATCTGGCTCGACCCGGCCAAG
CTGAACAGCTACCAGCTGACCCCGGGCGACGTGAGCAGCGCGATCCAGGCGCAGAACGTGCAGATTTCCTCC
GGCCAGCTCGGCGGCTTGCCGGCGGTCAAGGGCCAGCAGCTCAACGCCACCATCATCGGCAAGACCCGCCTG
CAGACCGCGGAGCAATTCGAGAACATCCTGCTCAAGGTCAATCCCGACGGTTCCCAGGTGCGCCTGAAGGAC
GTCGCCGATGTCGGCCTGGGCGGCCAGGACTACAGCATCAACGCGCAGTTCAACGGCAGCCCGGCGTCCGGT
ATCGCGATCAAGCTGGCCACCGGCGCCAACGCGCTGGATACCGCCAAGGCGATCCGCCAGACCATCGCCAAC
CTGGAACCGTTCATGCCGCAGGGCATGAAGGTGGTCTACCCGTACGACACCACCCCGGTGGTCTCGGCCTCG
ATCCATGAGGTAGTGAAGACCCTCGGCGAGGCGATCCTCCTCGTGTTCCTGGTGATGTACCTGTTCCTGCAG
AACTTCCGCGCCACGCTGATCCCGACCATCGCCGTACCGGTGGTGCTGCTGGGGACCTTCGGCGTGCTCGCC
GCGTTCGGCTTCTCGATCAACACCCTGACCATGTTCGGCATGGTGCTGGCCATCGGCTTGCTGGTGGACGAC
GCCATCGTGGTGGTGGAGAACGTCGAGCGGGTGATGGCCGAGGAAGGCCTGTCGCCAAGGGAGGCGGCGCGC
AAGTCCATGGGCCAGATCCAGGGCGCGCTGGTCGGTATCGCCATGGTGCTCTCGGCGGTATTCCTGCCGATG
GCGTTCTTCGGCGGCTCCACCGGGGTGATCTACCGGCAGTTCTCCATCACCATCGTGTCGGCCATGGCCCTC
TCGGTGATCGTGGCGCTGATCCTCACCCCGGCGCTCTGCGCGACCATGCTCAAGCCGATCGAGAAAGGCGAC
CATGGCGAGCACAAGGGCGGCTTCTTCGGCTGGTTCAACCGGATGTTCCTTTCCACCACCCACGGCTACGAG
CGGGGCGTGGCGTCGATCCTCAAGCATCGCGCGCCGTACCTGCTGATCTACGTGGTGATCGTGGCCGGGATG
ATCTGGATGTTCACCCGCATTCCCACCGCGTTCCTCCCCGACGAGGACCAGGGCGTACTGTTCGCCCAGGTA
CAGACCCCGCCGGGCTCCAGTGCCGAGCGTACCCAGGTGGTGGTGGACTCGATGCGCGAATACCTGCTGGAG
AAGGAAAGCTCTTCGGTCAGCTCGGTGTTCACCGTGACCGGCTTCAACTTCGCCGGCCGCGGCCAGAGTTCG
GGCATGGCGTTCATCATGCTCAAGCCCTGGGAAGAGCGTCCCGGTGGTGAGAACAGCGTGTTCGAGCTGGCC
AAGCGCGCGCAGATGCACTTCTTCAGCTTCAAGGACGCGATGGTGTTCGCCTTCGCGCCGCCGTCGGTACTG
GAACTGGGTAACGCCACCGGCTTCGACCTGTTCCTCCAGGACCAGGCGGGCGTCGGCCACGAGGTCCTGCTC
CAGGCGCGCAACAAGTTCCTCATGCTCGCCGCGCAGAACCCGGCGCTGCAACGCGTGCGCCCCAACGGCATG
AGCGACGAACCGCAGTACAAGCTGGAGATCGACGACGAGAAGGCCAGCGCCCTCGGCGTGTCCCTCGCCGAC
ATCAACAGCACCGTGTCCATCGCCTGGGGTTCCAGCTACGTCAACGACTTCATCGACCGTGGCCGGGTCAAG
CGGGTCTACCTGCAGGGCAGGCCGGACGCGCGGATGAACCCGGACGACCTGAGCAAGTGGTACGTGCGCAAC
GACAAGGGCGAGATGGTGCCGTTCAACGCCTTCGCCACCGGCAAGTGGGAATACGGTTCGCCGAAGCTGGAG
CGCTACAATGGCGTGCCGGCGATGGAGATCCTCGGCGAGCCGGCGCCCGGCCTGAGTTCCGGTGACGCCATG
GCGGCGGTCGAGGAGATCGTCAAGCAATTGCCGAAAGGCGTTGGCTACTCCTGGACCGGCCTGTCCTACGAG
GAGCGCTTGTCCGGCTCGCAGGCGCCGGCGCTGTATGCGCTGTCGCTGCTGGTGGTGTTCCTCTGCCTGGCG
GCCCTGTACGAAAGCTGGTCGATTCCGTTCTCGGTGATGCTGGTGGTGCCGTTGGGCGTGATCGGCGCGCTG
CTGGCGACGTCCATGCGCGGCCTGTCTAACGACGTGTTCTTCCAGGTGGGCCTGTTGACGACCATCGGCCTG
TCGGCGAAGAACGCCATTCTCATCGTGGAGTTCGCCAAGGAGCTGCACGAGCAGGGCAAGGGCATCGTCGAG
GCGGCCATCGAGGCCTGCCGCATGCGTCTGCGGCCGATCGTGATGACCTCCCTGGCGTTCATCCTTGGTGTG
GTTCCGCTGGCGATCTCCACCGGCGCCGGCTCGGGCAGCCAGCATGCGATCGGTACCGGCGTGATCGGCGGC
ATGGTCACTGCGACCGTCCTGGCGATCTTCTGGGTACCGCTGTTCTACGTGGCGGTCAGCACGCTGTTCAAG
GACGAGGCGTCCAAGCAGCAAGCGTCCGTCGAAAAGGGGCAATGA
Translation:
MSKFFIDRPIFAWVIALVIMLAGGLSILSLPVNQYPAIAPPAIAVQVSYPGASAETVQDTVVQVIEQQMNGI
DNLRYISSESNSDGSMTITVTFEQGTDPDIAQVQVQNKLQLATPLLPQEVQRQGIRVTKAVKNFLMVVGVVS
TDGSMTKEDLSNYIVSNIQDPLSRTKGVGDFQVFGSQYSMRIWLDPAKLNSYQLTPGDVSSAIQAQNVQISS
GQLGGLPAVKGQQLNATIIGKTRLQTAEQFENILLKVNPDGSQVRLKDVADVGLGGQDYSINAQFNGSPASG
IAIKLATGANALDTAKAIRQTIANLEPFMPQGMKVVYPYDTTPVVSASIHEVVKTLGEAILLVFLVMYLFLQ
NFRATLIPTIAVPVVLLGTFGVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLSPREAAR
KSMGQIQGALVGIAMVLSAVFLPMAFFGGSTGVIYRQFSITIVSAMALSVIVALILTPALCATMLKPIEKGD
HGEHKGGFFGWFNRMFLSTTHGYERGVASILKHRAPYLLIYVVIVAGMIWMFTRIPTAFLPDEDQGVLFAQV
QTPPGSSAERTQVVVDSMREYLLEKESSSVSSVFTVTGFNFAGRGQSSGMAFIMLKPWEERPGGENSVFELA
KRAQMHFFSFKDAMVFAFAPPSVLELGNATGFDLFLQDQAGVGHEVLLQARNKFLMLAAQNPALQRVRPNGM
SDEPQYKLEIDDEKASALGVSLADINSTVSIAWGSSYVNDFIDRGRVKRVYLQGRPDARMNPDDLSKWYVRN
DKGEMVPFNAFATGKWEYGSPKLERYNGVPAMEILGEPAPGLSSGDAMAAVEEIVKQLPKGVGYSWTGLSYE
ERLSGSQAPALYALSLLVVFLCLAALYESWSIPFSVMLVVPLGVIGALLATSMRGLSNDVFFQVGLLTTIGL
SAKNAILIVEFAKELHEQGKGIVEAAIEACRMRLRPIVMTSLAFILGVVPLAISTGAGSGSQHAIGTGVIGG
MVTATVLAIFWVPLFYVAVSTLFKDEASKQQASVEKGQ*
AnnotationID:70GeneID:60
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 09:09:23
Gene Name:mexB
Confidence Code:1
GeneProduct:RND multidrug efflux transporter MexB
Cell Localization:()
Synonyms:acrB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:acriflavine resistance protein B
Functional Category Confidence Code:5
COGs:pfam00873,COG0841
Secondary Functional Category(ies):antibiotic resistance and susceptibility
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67008.1|AE016329_3 (AE016779) multidrug/solvent RND transporter TtgB [Pseudomonas putida
            KT2440]
          Length = 1050

 Score = 1643 bits (4255), Expect = 0.0
 Identities = 818/1043 (78%), Positives = 940/1043 (90%), Gaps = 1/1043 (0%)

>gb|AAO57755.1| (AE016871) AcrB/AcrD/AcrF family protein [Pseudomonas syringae pv.
            tomato str. DC3000]
          Length = 1044

 Score = 1628 bits (4217), Expect = 0.0
 Identities = 827/1046 (79%), Positives = 931/1046 (89%), Gaps = 2/1046 (0%)

>gb|AAL19429.1| (AE008717) RND family, acridine efflux pump [Salmonella typhimurium
            LT2]
          Length = 1049

 Score = 1467 bits (3797), Expect = 0.0
 Identities = 732/1047 (69%), Positives = 874/1047 (83%), Gaps = 4/1047 (0%)








 
Structural Features:
pfam00873, ACR_tran, AcrB/AcrD/AcrF family. 
CD-Length = 1017 residues,  99.9% aligned
Score = 1282 bits (3320), Expect = 0.0
Identities = 551/1031 (53%), Positives = 713/1031 (69%), Gaps = 19/1031 (1%)

COG0841, AcrB, Cation/multidrug efflux pump [Defense mechanisms]
CD-Length = 1009 residues,  99.9% aligned
Score = 1094 bits (2831), Expect = 0.0
Identities = 575/1033 (55%), Positives = 733/1033 (70%), Gaps = 28/1033 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:123




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17265




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33751




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:60_cdd.html


    		




 
URL:

















    ReferenceID:35361




  
Author/Investigator(s):
        Gotoh N, Tsujimoto H, Poole K, Yamagishi J, Nishino T.





  
Title:
        The outer membrane protein OprM of Pseudomonas aeruginosa is encoded by oprK of the mexA-mexB-oprK multidrug resistance operon.




  
PubMed:
        8585747


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1995 Nov;39(11):2567-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8585747

















    ReferenceID:35362




  
Author/Investigator(s):
        Poole K, Krebes K, McNally C, Neshat S.





  
Title:
        Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon.




  
PubMed:
        8226684


MedLine:
        




    
Source:
J Bacteriol. 1993 Nov;175(22):7363-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8226684









           
Homologs By Global Alignment 

           

       



     
Gene ID:60
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
113
                           PA0426_tr
                           translation of PA0426
                           1046
                           99.99
                           99.99
                           0
                           5253.0
     

     
     
12487
                           gb|AAG03815.1|AE004479_10
                           (AE004479) RND multidrug efflux transporter MexB [Pseudomonas aeruginosa PAO1]
                           1046
                           99.99
                           99.99
                           0
                           5253.0
     

     
     
12488
                           gb|AAA74437.1|
                           (L11616) envD homologue; ORFB [Pseudomonas aeruginosa]
                           1046
                           99.90
                           99.90
                           0
                           5248.0
     

     
     
12489
                           gb|AAQ92181.1|
                           (AY349612) inner membrane pump protein [Pseudomonas fluorescens]
                           1049
                           79.79
                           90.84
                           4
                           4342.0
     

     
     
12493
                           gb|AAO57755.1|
                           (AE016871) AcrB/AcrD/AcrF family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           1048
                           79.10
                           89.02
                           6
                           4221.0
     

     






      

          
GC ORFID: 43062How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0426Glimmer Score: 

          
Start: 6289284Stop: 6292424

          
Length: 3141


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0426 translation of PA0426Homolog Bit Score 2028.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6178 (ORF: BLASTX 1630.0), t_PA2018 (ORF: BLASTX 1016.0), t_PA4598 (ORF: BLASTX 1007.0), t_PA2494 (ORF: BLASTX 797.0), psyr_15may02_Scaffold7_revised_gene7199 (ORF: BLASTX 787.0), psyr_15may02_Scaffold7_revised_gene7337 (ORF: BLASTX 130.0), t_PA0426 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGAAGTTTTTCATTGATAGGCCCATTTTCGCGTGGGTGATCGCCTTGGTGATCATGCTCGCGGGCGGC 
CTGTCGATCCTCAGTCTGCCGGTCAACCAGTACCCGGCCATCGCCCCGCCGGCCATCGCCGTGCAGGTGAGC 
TACCCGGGCGCCTCGGCCGAGACGGTGCAGGACACCGTGGTCCAGGTGATCGAGCAGCAGATGAACGGGATC 
GACAATCTGCGCTACATCTCCTCGGAGAGTAACTCCGACGGCAGCATGACCATCACCGTGACCTTCGAACAG 
GGCACCGACCCCGACATCGCCCAGGTCCAGGTGCAGAACAAGCTGCAACTGGCCACCCCGCTGCTGCCGCAG 
GAAGTGCAGCGCCAGGGGATCCGGGTGACCAAGGCGGTGAAGAACTTCCTCATGGTGGTCGGTGTGGTTTCC 
ACCGACGGCAGCATGACCAAGGAAGACCTGTCGAACTACATCGTTTCCAACATCCAGGACCCGCTCTCGCGG 
ACCAAGGGCGTCGGTGACTTCCAGGTGTTCGGCTCGCAGTACTCGATGCGCATCTGGCTCGACCCGGCCAAG 
CTGAACAGCTACCAGCTGACCCCGGGCGACGTGAGCAGCGCGATCCAGGCGCAGAACGTGCAGATTTCCTCC 
GGCCAGCTCGGCGGCTTGCCGGCGGTCAAGGGCCAGCAGCTCAACGCCACCATCATCGGCAAGACCCGCCTG 
CAGACCGCGGAGCAATTCGAGAACATCCTGCTCAAGGTCAATCCCGACGGTTCCCAGGTGCGCCTGAAGGAC 
GTCGCCGATGTCGGCCTGGGCGGCCAGGACTACAGCATCAACGCGCAGTTCAACGGCAGCCCGGCGTCCGGT 
ATCGCGATCAAGCTGGCCACCGGCGCCAACGCGCTGGATACCGCCAAGGCGATCCGCCAGACCATCGCCAAC 
CTGGAACCGTTCATGCCGCAGGGCATGAAGGTGGTCTACCCGTACGACACCACCCCGGTGGTCTCGGCCTCG 
ATCCATGAGGTAGTGAAGACCCTCGGCGAGGCGATCCTCCTCGTGTTCCTGGTGATGTACCTGTTCCTGCAG 
AACTTCCGCGCCACGCTGATCCCGACCATCGCCGTACCGGTGGTGCTGCTGGGGACCTTCGGCGTGCTCGCC 
GCGTTCGGCTTCTCGATCAACACCCTGACCATGTTCGGCATGGTGCTGGCCATCGGCTTGCTGGTGGACGAC 
GCCATCGTGGTGGTGGAGAACGTCGAGCGGGTGATGGCCGAGGAAGGCCTGTCGCCAAGGGAGGCGGCGCGC 
AAGTCCATGGGCCAGATCCAGGGCGCGCTGGTCGGTATCGCCATGGTGCTCTCGGCGGTATTCCTGCCGATG 
GCGTTCTTCGGCGGCTCCACCGGGGTGATCTACCGGCAGTTCTCCATCACCATCGTGTCGGCCATGGCCCTC 
TCGGTGATCGTGGCGCTGATCCTCACCCCGGCGCTCTGCGCGACCATGCTCAAGCCGATCGAGAAAGGCGAC 
CATGGCGAGCACAAGGGCGGCTTCTTCGGCTGGTTCAACCGGATGTTCCTTTCCACCACCCACGGCTACGAG 
CGGGGCGTGGCGTCGATCCTCAAGCATCGCGCGCCGTACCTGCTGATCTACGTGGTGATCGTGGCCGGGATG 
ATCTGGATGTTCACCCGCATTCCCACCGCGTTCCTCCCCGACGAGGACCAGGGCGTACTGTTCGCCCAGGTA 
CAGACCCCGCCGGGCTCCAGTGCCGAGCGTACCCAGGTGGTGGTGGACTCGATGCGCGAATACCTGCTGGAG 
AAGGAAAGCTCTTCGGTCAGCTCGGTGTTCACCGTGACCGGCTTCAACTTCGCCGGCCGCGGCCAGAGTTCG 
GGCATGGCGTTCATCATGCTCAAGCCCTGGGAAGAGCGTCCCGGTGGTGAGAACAGCGTGTTCGAGCTGGCC 
AAGCGCGCGCAGATGCACTTCTTCAGCTTCAAGGACGCGATGGTGTTCGCCTTCGCGCCGCCGTCGGTACTG 
GAACTGGGTAACGCCACCGGCTTCGACCTGTTCCTCCAGGACCAGGCGGGCGTCGGCCACGAGGTCCTGCTC 
CAGGCGCGCAACAAGTTCCTCATGCTCGCCGCGCAGAACCCGGCGCTGCAACGCGTGCGCCCCAACGGCATG 
AGCGACGAACCGCAGTACAAGCTGGAGATCGACGACGAGAAGGCCAGCGCCCTCGGCGTGTCCCTCGCCGAC 
ATCAACAGCACCGTGTCCATCGCCTGGGGTTCCAGCTACGTCAACGACTTCATCGACCGTGGCCGGGTCAAG 
CGGGTCTACCTGCAGGGCAGGCCGGACGCGCGGATGAACCCGGACGACCTGAGCAAGTGGTACGTGCGCAAC 
GACAAGGGCGAGATGGTGCCGTTCAACGCCTTCGCCACCGGCAAGTGGGAATACGGTTCGCCGAAGCTGGAG 
CGCTACAATGGCGTGCCGGCGATGGAGATCCTCGGCGAGCCGGCGCCCGGCCTGAGTTCCGGTGACGCCATG 
GCGGCGGTCGAGGAGATCGTCAAGCAATTGCCGAAAGGCGTTGGCTACTCCTGGACCGGCCTGTCCTACGAG 
GAGCGCTTGTCCGGCTCGCAGGCGCCGGCGCTGTATGCGCTGTCGCTGCTGGTGGTGTTCCTCTGCCTGGCG 
GCCCTGTACGAAAGCTGGTCGATTCCGTTCTCGGTGATGCTGGTGGTGCCGTTGGGCGTGATCGGCGCGCTG 
CTGGCGACGTCCATGCGCGGCCTGTCTAACGACGTGTTCTTCCAGGTGGGCCTGTTGACGACCATCGGCCTG 
TCGGCGAAGAACGCCATTCTCATCGTGGAGTTCGCCAAGGAGCTGCACGAGCAGGGCAAGGGCATCGTCGAG 
GCGGCCATCGAGGCCTGCCGCATGCGTCTGCGGCCGATCGTGATGACCTCCCTGGCGTTCATCCTTGGTGTG 
GTTCCGCTGGCGATCTCCACCGGCGCCGGCTCGGGCAGCCAGCATGCGATCGGTACCGGCGTGATCGGCGGC 
ATGGTCACTGCGACCGTCCTGGCGATCTTCTGGGTACCGCTGTTCTACGTGGCGGTCAGCACGCTGTTCAAG 
GACGAGGCGTCCAAGCAGCAAGCGTCCGTCGAAAAGGGGCAATGA