ViewGene

Locus Name:
GeneID:
Location:

GeneID: 6

TrivialName: PA14_34840

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_34840

PAO1 Ortholog Locus: PA2302

Sequence Length: 6378

Protein Length: 2125

Start: 3096787

Stop: 3103164

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG05690.1|AE004656_2 (AE004656) probable non-ribosomal peptide synthetase [Pseudomonas aeruginosa PAO1] Identities = 2091/2125 (98%), Positives = 2099/2125 (98%), Gaps = 1/2125 (0%)

Sequence:

ATGAGTGCGTCAGAAGACCTGCAATCCGCTGTGCAACCGGCCGCGAGCGAAGCGCTCGAAGGATTCCCGCTG
TCCCCCTTGCAGACCCGCGCCTGGCGCCGTCATGCCGAGCGGCCGGAAAATACCGTTGTCGGCGTGCGCCTG
CACGCCCCGGCCGATCCCGCGGCGACGCTGGAGCGGCTGCGCCGGGCGCTGGACGGCGAGGCGCAACTGCGC
GTGGCTTACCGGACGATGCCGGGCATGAGCCTGCCGGTGCAGGTACTGGATGGACGCGCGGCCGATCTGCTG
GTCGAGCGCCTGCCGGGAGACGGCGACTGGGCCGGGCGCTTCGCGCGCGAAAGCGCACGTCTCGCCGCTGCG
CCCCTGGGCGGGGAAGGCCAGCCGGTGCTGGCGCTCGGCCTGCTGCTGGACGCCGCCGGAGAGACGCTCCAG
GGGCTGTTGCTGGCGGCGCCGGCGTTCGTCGTCGATGCGGCCAGCCTGGCGGCGCTGCTGCGCCGCGGCCTG
GGGCAGGCCGGCCCGGCGAGCGCGGACGAGGGGGCCGAGGCGCTGCTGTTCCAGCATTTCTCCGAATGGGCC
AACGAGGCGCTGGCCGGCGAAGACGGCGAAAGCGCCAGCGGCTACTGGCGCGAGCAGGCGGCCGTTGCGGCG
GAGAGTCCGCTGGCGCTGGCGGACGACCTGGGCGAAGGCGAGTGGACGGCGCGGCGCCTGCTGCCGCGCGCG
CTGCTCGAACGCCTGGCCGCCAACGGCTTGCCGGAGGCGGCCGCCCTGCTGGCCTGGACCCAGGTCGCCGGG
CAGTTCCAGGGCGACGAGGGACTCCCGCTGGAAATGGCGCGACTGGTCTCGGGGCGCCTGTTCAACGAGTTC
GCCGAGCTGGCCGGACCGTTCGCCGGGGTCGCGCCGCTGTGCCTGGAGAATGTCCGCGCGGGCAGCGTCGGC
GAGCGGCTCGACGCCCTCCAGGCGGCGATCCTCGCCCAGGAGGAGGCAGCGGCCCTGCGCGATCCCTTTGCC
CCCGACTGGCCGCTCGCCGAGTTGGGCTTCGCCTGGCTGGCGGGCGAACTGGATGGCGCCGGGGTGGCCGAG
CTGGATTGCCGGCAGCCGCCGCTGGGCGGGTTCCTCGAGTTGCAGGTGCTGCCCCACGGCGAAGGCCGGCTG
GCCAGCCTGCGGGTCCGCCGCGACCATGACGGAACGCTGGCCGGGCGCTTGCTCGACGCCTGGGTCGAATGC
CTGGAAAGCATCGCCGCCGACAGGCAACTGCCACTGGCCGGGCTGCCGTTGATCGGCGCGGCCGAGCGCGAG
CGCTACCAGGCCTGGCAGGGCGAGCGCGTGGAGCCCGCGCCGGTGGAATCCCTGGTGGCCGCGTTCGATCTG
CGCGCCGCCCTGCAGCCGCAGGCGCCGGCGTTGCTGGATGCCCACGGCAGCCTGGATTTCGCCACGCTGCGC
GCGCGCAGCGAAGCGGTCGCCGAAGCGCTGCTGGCTGCCGGCGTGCGGCCCGGCCAGGCGGTGGCGGTGATG
ACCGGGCGCAACCGCGAGGCGATCGTCGCCCTGCTCGGGGTGATGCGCGCGGCGGCGGTGTACACCCCGGTC
AATCCGGAGTTTCCGGCGGCGCGGGTGGAGCGGATGCGCGAAGCGGGCGGGATCGTCTTCGCCCTTGCCGAT
GCCGAGTGCGCCGGGCGCGCCCGCGAGGCCTTCGCCGGGGCCTGCCTGGACCTGTCGACGCTGCCGCTTGCC
GGCAGCGGCATGAGCCTGCCGGCGCCGGGCGGGCGCGATGCGGCCTACATGATCTTCACCTCGGGCACCAGC
GGCCAGCCCAAAGGCGTGGTGGTCGAGCACGCCAGCGCGCTCAACCTGTCCCAGGCCCTGGCGCGCACGGTA
TACGCGAACGTGGTGGGCGAGGGCCTGCGGGTGACGGTCAACGCGCCGTTCTCCTTCGATTCCTCGATCAAG
CAGATTCTCCAGTTGCTCTCCGGCCATTGCCTGGTCCCGGTGCCGCAGGAGGTGCGCAGCGATCCGCAGCGG
ATGCTGGGGTTCCTCGAAGAACGGCGCATCGACGTGCTCGACTGCACCCCGTCGCTGTTCCGCCTGCTGCTC
CAGGCCGGCCTCGACGATGCCCATCCGGCGCTGCCCGGGTGCATCCTGGTAGGGGGCGAGCGCTTCGACGAA
GCGTCCTGGGAGGTCGCCGCCGGCTGGCGCCGCTGCCAGGTGTTCAATCTCTACGGTCCTACCGAAGCCACG
GTGAACGCCAGCCTGGCGCGGGTCGCCGAGCATGCGCGGCCGACCATCGGCCGCGCCCTGGCCAACGTCGAT
CTGCATGTGGTCGATGGCCTCGGTCGTCGCAAGACCCGTGGCGCCAGCGGCGAACTGTGGATCGGCGGCGCC
GGGGTGGCGCGCGGCTATGCCGGCGACGCCGGCGAGGCGGCCGGGCGCTTCCTCGAGGAGGGCTGGCCGGGC
AGCGGCCGCCTGTACCGCAGCGGCGACCTGGTGCGCTGGCGCGCCGACGGTTGCCTGGAGTTCCTCGGGCGG
ATCGACGAACAGGTGAAGATCAACGGCTACCGCATCGAACTGGGCGAGATCCGCAGCGCGTTGCTGGAACAC
CCGGCAGTGGGCGAGGCGGCGGTACTCACCGACGAGGCCGATGCGGCCGAGCCGGGCACGGATCGCCGGATC
GTCGCCTTCGTCACCGCCGCCGGGGAGACCGCGGACGAGTCCTGGCTGGAAGTCGACCTGCCCAGCGGGCAC
CGGGTCGCCGGACTGAACCTCAACGAAACCGAGTACGTCTACCAGGAAATCTTCGTCGACGAGGTCTACAGC
CGCGACGGCATCGTCCTGCCGCCGGACGCGGTGGTCCTCGACGTCGGTGCCAACATCGGCCTGTTCTCGTTG
TACATCGCCAGCCGCGCGCCGCGCGCGCGAGTGGTCGCCTTCGAGCCGCTGGCACCGATCCGCCGGCGCCTG
GAGGCCAACCTCGGACGCTACGCACCGCAGGTCGAGGTATTCGGCATCGGCCTGTCCGACGCCGAGCGCGAG
GAAACCTTCACCTACTATCCGGGCTACTCGACCTTCTCCGGGATCGCCGAGTACGCCGACGCCAGCGGCGAA
CGCGACGTCATCCGACGCTACCTGAGCAACCAGGGCGAGGAGGGCGGGGCCAACCTGCTGCTGGACAACATC
GACGAAATCCTCGACGACCGCCTGCGCGCCGAAGCCCACCGCTGCCGCCTGCGCCGCCTCGACCAGGTGATC
GGCGAACTGGGCCTGGAGCGTATCGACCTGCTGAAGATCGACGTGCAGCGCGCGGAAATGGATGTGCTGCTC
GGTCTCGACGATGCGGCGCTGGCCAAGGTCCGGCAGATCGTCCTGGAGGTCCATGACAAGCGCGACGGTGCC
ACCGCCGGCCGCGCCGATGCCTTGAGCGACCTGCTGCGCCGCCATGGCTTCGAGGTGAGCATCCGTCAGGAC
GCGCTGCTGGAGGGTACCGACCGTTACAACTGCTACGCGGTGCGCCCGGGCTATGCCGAGTCGCTGGCCGAG
CGCATCGACTGGCGCGCGCTCGCGCCGCGCCCCGCCGCGGCCCTTGGCGGCGAGCTGAGCGAGCAGGCCCTG
CGTGGCTTCCTCGAGGCGCGCCTGCCGGCCTACATGCTGCCGAGCCGGATCGCCCGGGTCGAACGCCTGCCG
CTGACCGCCGAAGGCAAGCTCGACCGTCGCGCGCTGTTGGCGGCGCTGGCCGCcgaGGCGGCCGCGCAGCCC
CTgGaAGCGCCGGCCAATGCCACCGAGGCGGCCCTCCTGGATATCTGGAAGAGCGTGCTGAAACGCCCGGCG
ATTGGCGTCAGCGACAACTTCTTCCAGGTCGGCGGCGACTCCATCCGCCTGATCCAGATGCAGGTCATGGCG
CGCCAGGCCGGGCTTGCCTTTACCCTGCGCGACGTGTTCAACCACCAGAGCATCCGCGAACTGGCGCGCCTG
CTGGCCGCTCCGGCGAGCCCGGCGGATGCGGTCGGGACCTCGGCGCCGCAGACGCTGGAGCCGTTCGCCCTG
TTGTCGGCGGCGGAACGCAAGCGCCTGCCGGAGGGGCTCGACGACGCCTATCCGATGACCAGCCTGCAACAG
GGCATGCTCCTGCAAAGCGAGGCCAGCGGCGATCCACGGCTGTTGCACAACGTCGTCCTGCACGAGGTGCAT
GGACGCCTGGACAGCGAGTTGCTGGCGCGCGCCTGGGCGATCCTGATCGGCCGCCACGCGATCCTGCGTACC
GGCTTCGACCTGCACGGCGGCCAGGCGCCCTTGCAATGGGTCCACCCGGCCACGGCGGTCGCCGCCGAAGTG
CCGGTGCACGACCTGCGCGGCCTCGATGAGGAAGCCCGGCGCCTGCGCCTGCGTGCCTGGATCGAGGAAGAG
CAGGCCACCCCGTTCGACTGGAGCCGCCCACCGCTGGTGCGCCTCGCCGCGCTGGCGCTGGACGAGTGGCGC
TTCGCCCTGGGCGTCGCCGAACACCATAGCGTGCTGGACGGCTGGAGCCTGCAAAGCCTGGTGGACGAGCTG
CTGGCGGTCTACGCCGACCTCCTCGCCGGTGTCGTCGCGCGGGAAGCGGAAGCGCCCGCGGTAGGTTTCCGC
GACTACGTGGCGCTGGAGCGCGAGGCCGAGGCCAACGCCGGCTCGGCGCTGTTCTGGCTCGACTACCTGGCC
GGCGCCCGTTACCGGCCGTTGCCCGGCCTGGCGGAGGAGGGGCCCCGGCGGATGGCGGCGGTCCGCGTGGAC
GTGCCGGCCGACAGCCTGCCGCGCTTGCGCGCCCTGGCCGAACGCAGCGGCTTGCCCTTGCGTTCGTTGCTG
CTGGCGGCGCATGGCCGGGCGTTGTGCCGCTTCAGCGATGCCGACGAAGTGGTCAGCGGCTTCGTCAGCCAC
GGGCGCCCCGAGGAGCCGGGAGCGGACCGCCTGCTCGGCCTGTTCCTGAATACCCTGCCGTGCCGGCTGTCG
GCTTCCGTCGATCTGCTCGACAGCGCCCGTCGCGCATTCGACTACGAGCGCGCGAGCCTGGAGCATCGGCGC
CATCCGCTGGCGGCGATTCGCAGGCGCAACCGCGAGTTGCGCCTGGACAGCCTGTTCAACTTCGTCGATTTC
CACCAGGACGACGCCGCGCCGGCGGCGGGAGTAAGGCACGGCAGCATCCTCGACCAGGTGGTGGTGGACGTC
GACGTGCCGCTGGCGGTGGACTTCGAGGTGGCCGGCGAACGCCTCGAGGTGGGCTTCCAGTACGCCGCCGGA
CGTTTCCCCGCCGAGCGCGCCGAGGCATTGGCCGACGCCTACCGCGAGGCGTTGCTGGCGCTGCTCGAGGAC
CCGGCGCAGCCGCCCGCGGCGGTCCAGGCCGAGGACAGCGTGGAGCTGCGGCGGGTGCTCAAGGTGTTGTCC
CGGGTGCTCGGCCGGCCGCTGGCGGCCGACCAGGGCTTCGCCAGCGCCGGCGGGCATTCGCTGCTGGGCGTG
CAGGCGATCGCCGAATTGCGCCGGCTGACCGGCAGGCAACTGAGCCTGGGGCTGTTGCAGGGCGATCCGGAT
GCCCGCGAAGTGGTGCGCCGCTGCCATGTCGCCGACGCGCCGCCGTTGCCGCCCGCCACCGAGCGCGCCCGG
GCCCTGTGGTTGCAGCGCAGCGGGAGCGCGCAGCCGCGCCTGCGCCTGATCGCGCTGCCGCCCGCGGGCGGC
AACGCCGGCACTTTCCGCGGTTGGGACGCGCGCCTGCCGGCGGACGTGGAGCTGCTGGCGATCCAGTATCCG
GGGCGCCAGGAACGCCAGGACGAGCCGTTCGTCACCGATGTAGAGGCCATGCTCTGTGCCATCGACGACGCG
CTCCTGCCATTGCTCGACCGTCCGTTCGCCCTGGTCGGCGCCAGCCTCGGTGGCATGCTCGCCTACGAACTG
GCGGCACGCCTGGAAAGCCTGCACGGCCTGCGCGCCAGGCAGTTGTTCGTGATCAGCAGCCGCGCTCCGGGG
CCGGACCTGGAATACCCGCGCTTCCATGCGATGGGCGACGCCGAGTTGCTGCGAACCCTGCGCGAGTACGAC
GTGCTGCCGCTGGAAGTGCTCGACGACCCGGAGCTGCGCGAGATCAGCCTGGCCACCCTGCGCGCCGATTCG
CGCCTGGCCGCCGACTATCGCTACCGGCCGCGCGAGCCGTTGGCGATACCGATCACCGCGATCCTTGGCGAA
CAGGACCCGGGCGTCTCCAGGGCGGCCATCGACGGCTGGCGGCGGCACGCCAGCCGCTACGAGCTGGAGACT
CTGGCCGGCGGCCACGGCCTGGTGGTGACGGCGGCGGAGGAGGTCTGCGCGATCCTGCGGCAGCGCCTGGCG
CCCGATGTGCCTGGCGGCGTGCCGGCGAACCTGGCAACCTGA

Translation:

MSASEDLQSAVQPAASEALEGFPLSPLQTRAWRRHAERPENTVVGVRLHAPADPAATLERLRRALDGEAQLR
VAYRTMPGMSLPVQVLDGRAADLLVERLPGDGDWAGRFARESARLAAAPLGGEGQPVLALGLLLDAAGETLQ
GLLLAAPAFVVDAASLAALLRRGLGQAGPASADEGAEALLFQHFSEWANEALAGEDGESASGYWREQAAVAA
ESPLALADDLGEGEWTARRLLPRALLERLAANGLPEAAALLAWTQVAGQFQGDEGLPLEMARLVSGRLFNEF
AELAGPFAGVAPLCLENVRAGSVGERLDALQAAILAQEEAAALRDPFAPDWPLAELGFAWLAGELDGAGVAE
LDCRQPPLGGFLELQVLPHGEGRLASLRVRRDHDGTLAGRLLDAWVECLESIAADRQLPLAGLPLIGAAERE
RYQAWQGERVEPAPVESLVAAFDLRAALQPQAPALLDAHGSLDFATLRARSEAVAEALLAAGVRPGQAVAVM
TGRNREAIVALLGVMRAAAVYTPVNPEFPAARVERMREAGGIVFALADAECAGRAREAFAGACLDLSTLPLA
GSGMSLPAPGGRDAAYMIFTSGTSGQPKGVVVEHASALNLSQALARTVYANVVGEGLRVTVNAPFSFDSSIK
QILQLLSGHCLVPVPQEVRSDPQRMLGFLEERRIDVLDCTPSLFRLLLQAGLDDAHPALPGCILVGGERFDE
ASWEVAAGWRRCQVFNLYGPTEATVNASLARVAEHARPTIGRALANVDLHVVDGLGRRKTRGASGELWIGGA
GVARGYAGDAGEAAGRFLEEGWPGSGRLYRSGDLVRWRADGCLEFLGRIDEQVKINGYRIELGEIRSALLEH
PAVGEAAVLTDEADAAEPGTDRRIVAFVTAAGETADESWLEVDLPSGHRVAGLNLNETEYVYQEIFVDEVYS
RDGIVLPPDAVVLDVGANIGLFSLYIASRAPRARVVAFEPLAPIRRRLEANLGRYAPQVEVFGIGLSDAERE
ETFTYYPGYSTFSGIAEYADASGERDVIRRYLSNQGEEGGANLLLDNIDEILDDRLRAEAHRCRLRRLDQVI
GELGLERIDLLKIDVQRAEMDVLLGLDDAALAKVRQIVLEVHDKRDGATAGRADALSDLLRRHGFEVSIRQD
ALLEGTDRYNCYAVRPGYAESLAERIDWRALAPRPAAALGGELSEQALRGFLEARLPAYMLPSRIARVERLP
LTAEGKLDRRALLAALAAEAAAQPLEAPANATEAALLDIWKSVLKRPAIGVSDNFFQVGGDSIRLIQMQVMA
RQAGLAFTLRDVFNHQSIRELARLLAAPASPADAVGTSAPQTLEPFALLSAAERKRLPEGLDDAYPMTSLQQ
GMLLQSEASGDPRLLHNVVLHEVHGRLDSELLARAWAILIGRHAILRTGFDLHGGQAPLQWVHPATAVAAEV
PVHDLRGLDEEARRLRLRAWIEEEQATPFDWSRPPLVRLAALALDEWRFALGVAEHHSVLDGWSLQSLVDEL
LAVYADLLAGVVAREAEAPAVGFRDYVALEREAEANAGSALFWLDYLAGARYRPLPGLAEEGPRRMAAVRVD
VPADSLPRLRALAERSGLPLRSLLLAAHGRALCRFSDADEVVSGFVSHGRPEEPGADRLLGLFLNTLPCRLS
ASVDLLDSARRAFDYERASLEHRRHPLAAIRRRNRELRLDSLFNFVDFHQDDAAPAAGVRHGSILDQVVVDV
DVPLAVDFEVAGERLEVGFQYAAGRFPAERAEALADAYREALLALLEDPAQPPAAVQAEDSVELRRVLKVLS
RVLGRPLAADQGFASAGGHSLLGVQAIAELRRLTGRQLSLGLLQGDPDAREVVRRCHVADAPPLPPATERAR
ALWLQRSGSAQPRLRLIALPPAGGNAGTFRGWDARLPADVELLAIQYPGRQERQDEPFVTDVEAMLCAIDDA
LLPLLDRPFALVGASLGGMLAYELAARLESLHGLRARQLFVISSRAPGPDLEYPRFHAMGDAELLRTLREYD
VLPLEVLDDPELREISLATLRADSRLAADYRYRPREPLAIPITAILGEQDPGVSRAAIDGWRRHASRYELET
LAGGHGLVVTAAEEVCAILRQRLAPDVPGGVPANLAT*

AnnotationID:16 GeneID:6

AnnotatorUID: diggins

Modification Date/Time: 2005-03-04 12:12:35

Gene Name:

Confidence Code: 3

GeneProduct: putative non-ribosomal peptide synthetase

Cell Localization: ()

Synonyms: PA2302

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (20) Putative enzymes

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: pfam00501,COG1020,COG3208,COG0318

Secondary Functional Category(ies): central intermediary metabolism

EC Number:

Status: ACTIVE

Pathway:

Homology:

Structural Features:

pfam00501, AMP-binding, AMP-binding enzyme.
CD-Length = 412 residues,  99.8% aligned
Score =  287 bits (736), Expect = 7e-78
Identities = 155/419 (36%), Positives = 217/419 (51%), Gaps = 30/419 (7%)

COG1020, EntF, Non-ribosomal peptide synthetase modules and related proteins 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 642 residues,  99.4% aligned
Score =  287 bits (734), Expect = 1e-77
Identities = 208/651 (31%), Positives = 297/651 (45%), Gaps = 35/651 (5%)

COG3208, GrsT, Predicted thioesterase involved in non-ribosomal peptide biosynthesis 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 244 residues,  97.1% aligned
Score =  244 bits (624), Expect = 1e-64
Identities = 110/239 (46%), Positives = 135/239 (56%), Gaps = 4/239 (1%)

COG0318, CaiC, Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II 
[Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 534 residues,  88.8% aligned
Score =  198 bits (503), Expect = 8e-51
Identities = 127/486 (26%), Positives = 188/486 (38%), Gaps = 39/486 (8%)

Genomic Context:

Comment:

ReferenceID:15

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:17157

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:32488

Author/Investigator(s):

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: Misc (text/plain)

Data: 6_cdd.html

URL:

Homologs By Global Alignment

Gene ID:6

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

16 PA2302_tr translation of PA2302 2125 98.40 98.77 1 10564.0

8393 gb|AAG05690.1|AE004656_2 (AE004656) probable non-ribosomal peptide synthetase [Pseudomonas aeruginosa PAO1] 2125 98.40 98.77 1 10564.0

8395 emb|CAA66454.1| (X97860) peptide-synthetase [Amycolatopsis mediterranei] 2216 22.56 29.96 983 1314.0

8399 gb|AAF19815.1|AF188287_7 (AF188287) MtaG [Stigmatella aurantiaca] 2470 19.75 28.86 1065 663.0

8394 gb|AAG02349.1|AF210249_8 (AF210249) peptide synthetase NRPS11-10 [Streptomyces verticillus] 3313 18.59 25.74 1660 1356.0

GC ORFID: `43008`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA2302`	Glimmer Score:
Start: `1857129`	Stop: `1850752`
Length: `6378`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA2302 translation of PA2302`	Homolog Bit Score `4073.0`
Other Homologs: `t_PA4229 (ORF: BLASTX 130.0), t_PA2302 (ORF: GLIMMER)`
GC ORF Sequence
ATGAGTGCGTCAGAAGACCTGCAATCCGCTGTGCAACCGGCCGCGAGCGAAGCGCTCGAAGGATTCCCGCTG TCCCCCTTGCAGACCCGCGCCTGGCGCCGTCATGCCGAGCGGCCGGAAAATACCGTTGTCGGCGTGCGCCTG CACGCCCCGGCCGATCCCGCGGCGACGCTGGAGCGGCTGCGCCGGGCGCTGGACGGCGAGGCGCAACTGCGC GTGGCTTACCGGACGATGCCGGGCATGAGCCTGCCGGTGCAGGTACTGGATGGACGCGCGGCCGATCTGCTG GTCGAGCGCCTGCCGGGAGACGGCGACTGGGCCGGGCGCTTCGCGCGCGAAAGCGCACGTCTCGCCGCTGCG CCCCTGGGCGGGGAAGGCCAGCCGGTGCTGGCGCTCGGCCTGCTGCTGGACGCCGCCGGAGAGACGCTCCAG GGGCTGTTGCTGGCGGCGCCGGCGTTCGTCGTCGATGCGGCCAGCCTGGCGGCGCTGCTGCGCCGCGGCCTG GGGCAGGCCGGCCCGGCGAGCGCGGACGAGGGGGCCGAGGCGCTGCTGTTCCAGCATTTCTCCGAATGGGCC AACGAGGCGCTGGCCGGCGAAGACGGCGAAAGCGCCAGCGGCTACTGGCGCGAGCAGGCGGCCGTTGCGGCG GAGAGTCCGCTGGCGCTGGCGGACGACCTGGGCGAAGGCGAGTGGACGGCGCGGCGCCTGCTGCCGCGCGCG CTGCTCGAACGCCTGGCCGCCAACGGCTTGCCGGAGGCGGCCGCCCTGCTGGCCTGGACCCAGGTCGCCGGG CAGTTCCAGGGCGACGAGGGACTCCCGCTGGAAATGGCGCGACTGGTCTCGGGGCGCCTGTTCAACGAGTTC GCCGAGCTGGCCGGACCGTTCGCCGGGGTCGCGCCGCTGTGCCTGGAGAATGTCCGCGCGGGCAGCGTCGGC GAGCGGCTCGACGCCCTCCAGGCGGCGATCCTCGCCCAGGAGGAGGCAGCGGCCCTGCGCGATCCCTTTGCC CCCGACTGGCCGCTCGCCGAGTTGGGCTTCGCCTGGCTGGCGGGCGAACTGGATGGCGCCGGGGTGGCCGAG CTGGATTGCCGGCAGCCGCCGCTGGGCGGGTTCCTCGAGTTGCAGGTGCTGCCCCACGGCGAAGGCCGGCTG GCCAGCCTGCGGGTCCGCCGCGACCATGACGGAACGCTGGCCGGGCGCTTGCTCGACGCCTGGGTCGAATGC CTGGAAAGCATCGCCGCCGACAGGCAACTGCCACTGGCCGGGCTGCCGTTGATCGGCGCGGCCGAGCGCGAG CGCTACCAGGCCTGGCAGGGCGAGCGCGTGGAGCCCGCGCCGGTGGAATCCCTGGTGGCCGCGTTCGATCTG CGCGCCGCCCTGCAGCCGCAGGCGCCGGCGTTGCTGGATGCCCACGGCAGCCTGGATTTCGCCACGCTGCGC GCGCGCAGCGAAGCGGTCGCCGAAGCGCTGCTGGCTGCCGGCGTGCGGCCCGGCCAGGCGGTGGCGGTGATG ACCGGGCGCAACCGCGAGGCGATCGTCGCCCTGCTCGGGGTGATGCGCGCGGCGGCGGTGTACACCCCGGTC AATCCGGAGTTTCCGGCGGCGCGGGTGGAGCGGATGCGCGAAGCGGGCGGGATCGTCTTCGCCCTTGCCGAT GCCGAGTGCGCCGGGCGCGCCCGCGAGGCCTTCGCCGGGGCCTGCCTGGACCTGTCGACGCTGCCGCTTGCC GGCAGCGGCATGAGCCTGCCGGCGCCGGGCGGGCGCGATGCGGCCTACATGATCTTCACCTCGGGCACCAGC GGCCAGCCCAAAGGCGTGGTGGTCGAGCACGCCAGCGCGCTCAACCTGTCCCAGGCCCTGGCGCGCACGGTA TACGCGAACGTGGTGGGCGAGGGCCTGCGGGTGACGGTCAACGCGCCGTTCTCCTTCGATTCCTCGATCAAG CAGATTCTCCAGTTGCTCTCCGGCCATTGCCTGGTCCCGGTGCCGCAGGAGGTGCGCAGCGATCCGCAGCGG ATGCTGGGGTTCCTCGAAGAACGGCGCATCGACGTGCTCGACTGCACCCCGTCGCTGTTCCGCCTGCTGCTC CAGGCCGGCCTCGACGATGCCCATCCGGCGCTGCCCGGGTGCATCCTGGTAGGGGGCGAGCGCTTCGACGAA GCGTCCTGGGAGGTCGCCGCCGGCTGGCGCCGCTGCCAGGTGTTCAATCTCTACGGTCCTACCGAAGCCACG GTGAACGCCAGCCTGGCGCGGGTCGCCGAGCATGCGCGGCCGACCATCGGCCGCGCCCTGGCCAACGTCGAT CTGCATGTGGTCGATGGCCTCGGTCGTCGCAAGACCCGTGGCGCCAGCGGCGAACTGTGGATCGGCGGCGCC GGGGTGGCGCGCGGCTATGCCGGCGACGCCGGCGAGGCGGCCGGGCGCTTCCTCGAGGAGGGCTGGCCGGGC AGCGGCCGCCTGTACCGCAGCGGCGACCTGGTGCGCTGGCGCGCCGACGGTTGCCTGGAGTTCCTCGGGCGG ATCGACGAACAGGTGAAGATCAACGGCTACCGCATCGAACTGGGCGAGATCCGCAGCGCGTTGCTGGAACAC CCGGCAGTGGGCGAGGCGGCGGTACTCACCGACGAGGCCGATGCGGCCGAGCCGGGCACGGATCGCCGGATC GTCGCCTTCGTCACCGCCGCCGGGGAGACCGCGGACGAGTCCTGGCTGGAAGTCGACCTGCCCAGCGGGCAC CGGGTCGCCGGACTGAACCTCAACGAAACCGAGTACGTCTACCAGGAAATCTTCGTCGACGAGGTCTACAGC CGCGACGGCATCGTCCTGCCGCCGGACGCGGTGGTCCTCGACGTCGGTGCCAACATCGGCCTGTTCTCGTTG TACATCGCCAGCCGCGCGCCGCGCGCGCGAGTGGTCGCCTTCGAGCCGCTGGCACCGATCCGCCGGCGCCTG GAGGCCAACCTCGGACGCTACGCACCGCAGGTCGAGGTATTCGGCATCGGCCTGTCCGACGCCGAGCGCGAG GAAACCTTCACCTACTATCCGGGCTACTCGACCTTCTCCGGGATCGCCGAGTACGCCGACGCCAGCGGCGAA CGCGACGTCATCCGACGCTACCTGAGCAACCAGGGCGAGGAGGGCGGGGCCAACCTGCTGCTGGACAACATC GACGAAATCCTCGACGACCGCCTGCGCGCCGAAGCCCACCGCTGCCGCCTGCGCCGCCTCGACCAGGTGATC GGCGAACTGGGCCTGGAGCGTATCGACCTGCTGAAGATCGACGTGCAGCGCGCGGAAATGGATGTGCTGCTC GGTCTCGACGATGCGGCGCTGGCCAAGGTCCGGCAGATCGTCCTGGAGGTCCATGACAAGCGCGACGGTGCC ACCGCCGGCCGCGCCGATGCCTTGAGCGACCTGCTGCGCCGCCATGGCTTCGAGGTGAGCATCCGTCAGGAC GCGCTGCTGGAGGGTACCGACCGTTACAACTGCTACGCGGTGCGCCCGGGCTATGCCGAGTCGCTGGCCGAG CGCATCGACTGGCGCGCGCTCGCGCCGCGCCCCGCCGCGGCCCTTGGCGGCGAGCTGAGCGAGCAGGCCCTG CGTGGCTTCCTCGAGGCGCGCCTGCCGGCCTACATGCTGCCGAGCCGGATCGCCCGGGTCGAACGCCTGCCG CTGACCGCCGAAGGCAAGCTCGACCGTCGCGCGCTGTTGGCGGCGCTGGCCGCcgaGGCGGCCGCGCAGCCC CTgGaAGCGCCGGCCAATGCCACCGAGGCGGCCCTCCTGGATATCTGGAAGAGCGTGCTGAAACGCCCGGCG ATTGGCGTCAGCGACAACTTCTTCCAGGTCGGCGGCGACTCCATCCGCCTGATCCAGATGCAGGTCATGGCG CGCCAGGCCGGGCTTGCCTTTACCCTGCGCGACGTGTTCAACCACCAGAGCATCCGCGAACTGGCGCGCCTG CTGGCCGCTCCGGCGAGCCCGGCGGATGCGGTCGGGACCTCGGCGCCGCAGACGCTGGAGCCGTTCGCCCTG TTGTCGGCGGCGGAACGCAAGCGCCTGCCGGAGGGGCTCGACGACGCCTATCCGATGACCAGCCTGCAACAG GGCATGCTCCTGCAAAGCGAGGCCAGCGGCGATCCACGGCTGTTGCACAACGTCGTCCTGCACGAGGTGCAT GGACGCCTGGACAGCGAGTTGCTGGCGCGCGCCTGGGCGATCCTGATCGGCCGCCACGCGATCCTGCGTACC GGCTTCGACCTGCACGGCGGCCAGGCGCCCTTGCAATGGGTCCACCCGGCCACGGCGGTCGCCGCCGAAGTG CCGGTGCACGACCTGCGCGGCCTCGATGAGGAAGCCCGGCGCCTGCGCCTGCGTGCCTGGATCGAGGAAGAG CAGGCCACCCCGTTCGACTGGAGCCGCCCACCGCTGGTGCGCCTCGCCGCGCTGGCGCTGGACGAGTGGCGC TTCGCCCTGGGCGTCGCCGAACACCATAGCGTGCTGGACGGCTGGAGCCTGCAAAGCCTGGTGGACGAGCTG CTGGCGGTCTACGCCGACCTCCTCGCCGGTGTCGTCGCGCGGGAAGCGGAAGCGCCCGCGGTAGGTTTCCGC GACTACGTGGCGCTGGAGCGCGAGGCCGAGGCCAACGCCGGCTCGGCGCTGTTCTGGCTCGACTACCTGGCC GGCGCCCGTTACCGGCCGTTGCCCGGCCTGGCGGAGGAGGGGCCCCGGCGGATGGCGGCGGTCCGCGTGGAC GTGCCGGCCGACAGCCTGCCGCGCTTGCGCGCCCTGGCCGAACGCAGCGGCTTGCCCTTGCGTTCGTTGCTG CTGGCGGCGCATGGCCGGGCGTTGTGCCGCTTCAGCGATGCCGACGAAGTGGTCAGCGGCTTCGTCAGCCAC GGGCGCCCCGAGGAGCCGGGAGCGGACCGCCTGCTCGGCCTGTTCCTGAATACCCTGCCGTGCCGGCTGTCG GCTTCCGTCGATCTGCTCGACAGCGCCCGTCGCGCATTCGACTACGAGCGCGCGAGCCTGGAGCATCGGCGC CATCCGCTGGCGGCGATTCGCAGGCGCAACCGCGAGTTGCGCCTGGACAGCCTGTTCAACTTCGTCGATTTC CACCAGGACGACGCCGCGCCGGCGGCGGGAGTAAGGCACGGCAGCATCCTCGACCAGGTGGTGGTGGACGTC GACGTGCCGCTGGCGGTGGACTTCGAGGTGGCCGGCGAACGCCTCGAGGTGGGCTTCCAGTACGCCGCCGGA CGTTTCCCCGCCGAGCGCGCCGAGGCATTGGCCGACGCCTACCGCGAGGCGTTGCTGGCGCTGCTCGAGGAC CCGGCGCAGCCGCCCGCGGCGGTCCAGGCCGAGGACAGCGTGGAGCTGCGGCGGGTGCTCAAGGTGTTGTCC CGGGTGCTCGGCCGGCCGCTGGCGGCCGACCAGGGCTTCGCCAGCGCCGGCGGGCATTCGCTGCTGGGCGTG CAGGCGATCGCCGAATTGCGCCGGCTGACCGGCAGGCAACTGAGCCTGGGGCTGTTGCAGGGCGATCCGGAT GCCCGCGAAGTGGTGCGCCGCTGCCATGTCGCCGACGCGCCGCCGTTGCCGCCCGCCACCGAGCGCGCCCGG GCCCTGTGGTTGCAGCGCAGCGGGAGCGCGCAGCCGCGCCTGCGCCTGATCGCGCTGCCGCCCGCGGGCGGC AACGCCGGCACTTTCCGCGGTTGGGACGCGCGCCTGCCGGCGGACGTGGAGCTGCTGGCGATCCAGTATCCG GGGCGCCAGGAACGCCAGGACGAGCCGTTCGTCACCGATGTAGAGGCCATGCTCTGTGCCATCGACGACGCG CTCCTGCCATTGCTCGACCGTCCGTTCGCCCTGGTCGGCGCCAGCCTCGGTGGCATGCTCGCCTACGAACTG GCGGCACGCCTGGAAAGCCTGCACGGCCTGCGCGCCAGGCAGTTGTTCGTGATCAGCAGCCGCGCTCCGGGG CCGGACCTGGAATACCCGCGCTTCCATGCGATGGGCGACGCCGAGTTGCTGCGAACCCTGCGCGAGTACGAC GTGCTGCCGCTGGAAGTGCTCGACGACCCGGAGCTGCGCGAGATCAGCCTGGCCACCCTGCGCGCCGATTCG CGCCTGGCCGCCGACTATCGCTACCGGCCGCGCGAGCCGTTGGCGATACCGATCACCGCGATCCTTGGCGAA CAGGACCCGGGCGTCTCCAGGGCGGCCATCGACGGCTGGCGGCGGCACGCCAGCCGCTACGAGCTGGAGACT CTGGCCGGCGGCCACGGCCTGGTGGTGACGGCGGCGGAGGAGGTCTGCGCGATCCTGCGGCAGCGCCTGGCG CCCGATGTGCCTGGCGGCGTGCCGGCGAACCTGGCAACCTGA