ViewGene
Locus Name:
GeneID:
Location:

GeneID:596
TrivialName: PA14_18330
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18330
PAO1 Ortholog Locus:PA3556
Sequence Length:1650
Protein Length:549
Start: 1573239
Stop: 1571590
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06944.1|AE004776_7 (AE004776) inner membrane L-Ara4N transferase ArnT [Pseudomonas aeruginosa PAO1]
Identities = 544/549 (99%), Positives = 546/549 (99%)
Sequence:
ATGAGCCGCCGCCAGACCTGGTCGCTGCTGCTGATCGCCTTCGGCCTGTTCTACCTGGTTCCATTGAGCAAC
CATGGCCTGTGGATTCCCGACGAGACCCGCTACGCGCAGATCAGCCAGGCGATGCTGCtGGGCGGCGACTGG
GTGTCGCCGCACTTCCTCGGCCTGCGCTACTTCGAGAAGCCGGTCGCCGGCTACTGGATGATCGCCCTCGGC
CAGGCGGTCTTCGGCGAAAACCTGTTCGGCGTGCGCATCGCCTCGGTCGTCGCCACCGCCCTCAGCGTCCTG
CTCGCCTACCTGCTGGCCCGCCGCCTGTGGCGCGACCCGCGCACCAGCCTGGCGTGCGCGCTGCTCTACGCC
AGCTTCGGCTTGATCGCCGGGCAGTCCGGCTATGCCAACCTCGACCCGCAATTCACCTTCTGGGTCAACCTG
AGCCTGGTGGCGCTGTGGTACGCCCTCGACGCGGGGAGCCGCCGCGCGCGCCTGCTCGGCTGGATCCTGCTC
GGCCTCGCCTGCGGCATGGGCTTCCTGACCAAGGGCTTCCTCGCCTGGCTGCTGCCGGTGCTGGTCGCCCTG
CCCTACATGCTCTGGCAGCGGCGCTGGCGCGAGCTGCTTGGCTACGGCGCGCTGGCGGTGCTGGCGGCGCTG
CTCGTCTGCCTGCCCTGGGCGCTCGCCGTGCATGCGCGGGAAGCGGACTACTGGCGGTTCTTCTTCTGGCAC
GAACACATCCGCCGCTTCGCCGGCGAAGACGCCCAGCACTCTCGCCCGTGGTGGTTCTACCTGCCGTTGCTG
GTGGTCGCCTGCCTGCCCTGGAGCGGCCTGCTGCCGAGCGCTCTGCGCCAGGCCTGGCACGAGCGGCGCCAG
GCGCCGGTGGTCTTCCTGGCGCTATGGCTGCTGTTGCCGCTGGCGTTCTTCAGCCTGAGCCGGGGCAAGCTG
CCGACCTACATCATGCCCTGCCTGCTGCCGCTGGCACTGCTCATGGGCCACGCCCTGGTGCAGCGGCTGCGC
CTGGGGAACAGCGTCGCGCTGCGCGGCAACGGGCTGCTCAACCTGGGCCTGGCGCTCCTCGCGCTGGCGGCC
CTGGCCTACCTGCAACTGCGCAAGCCGGTGTACCAGGAAGAACCCTTCGAGCTGTTCCTGGTCCTGCTGGTG
ATCGGCGCCTGGGCCGCCGCCGGCCTCGCCCAGTGGCGCTACCCGCTACGCGCCTGGGCCGCGCCGCTGCTG
GCGAGCTGGGTGCTGATCGCGCTGCTGCCGGCAGCGATGCCCAACCATGTGGTGCAGAACAAGACCCCCGAC
CTGTTCGTCGCCGAACACCTCGACGAACTGACCGGCGCCCGCCATCTGCTGAGCAACGACCTCGGCGCCGCC
TCGGCCCTCGCCTGGCGCCTGCGTCGTAGCGACGTGACGCTCTACGACACGCGCGGCGAGCTGAAGTACGGC
CTCTCCTACCCCGAGCACAGCCAGCGCAGCGTGCCCTTGGCAGACATCCGCCAGTGGCTCTGGCGGGCACGC
CAGGACGGCTCGGTTGCCGTGCTGCTGCGGATCAACAGCGCCAGCGACCGCTACCAGCTGGCGCTGTTGCCG
GGCGACGGCGAACGCTACCGGAACGGCAACCTGGTCCTGGCGATCCTGCCGCAGGTGCGCCCATGA
Translation:
MSRRQTWSLLLIAFGLFYLVPLSNHGLWIPDETRYAQISQAMLLGGDWVSPHFLGLRYFEKPVAGYWMIALG
QAVFGENLFGVRIASVVATALSVLLAYLLARRLWRDPRTSLACALLYASFGLIAGQSGYANLDPQFTFWVNL
SLVALWYALDAGSRRARLLGWILLGLACGMGFLTKGFLAWLLPVLVALPYMLWQRRWRELLGYGALAVLAAL
LVCLPWALAVHAREADYWRFFFWHEHIRRFAGEDAQHSRPWWFYLPLLVVACLPWSGLLPSALRQAWHERRQ
APVVFLALWLLLPLAFFSLSRGKLPTYIMPCLLPLALLMGHALVQRLRLGNSVALRGNGLLNLGLALLALAA
LAYLQLRKPVYQEEPFELFLVLLVIGAWAAAGLAQWRYPLRAWAAPLLASWVLIALLPAAMPNHVVQNKTPD
LFVAEHLDELTGARHLLSNDLGAASALAWRLRRSDVTLYDTRGELKYGLSYPEHSQRSVPLADIRQWLWRAR
QDGSVAVLLRINSASDRYQLALLPGDGERYRNGNLVLAILPQVRP*
AnnotationID:605GeneID:596
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 05:05:45
Gene Name:arnT
Confidence Code:2
GeneProduct:4-amino-4-deoxy-L-arabinose lipid A transferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:inner membrane L-Ara4N transferase ArnT
Functional Category Confidence Code:5
COGs:pfam02366,COG1807
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN81253.1|AE016763_212 (AE016763) Hypothetical protein yfbI [Escherichia coli CFT073]
          Length = 550

 Score =  446 bits (1146), Expect = e-124
 Identities = 219/526 (41%), Positives = 320/526 (60%), Gaps = 7/526 (1%)

>gb|AAD20796.1| (AF071082) melittin resistance protein PqaB [Salmonella typhi]
          Length = 548

 Score =  446 bits (1146), Expect = e-124
 Identities = 230/555 (41%), Positives = 338/555 (60%), Gaps = 14/555 (2%)

>gb|AAL34393.1| (AY057444) 4-amino-4-deoxy-L-arabinose lipid A transferase
           [Salmonella typhimurium]
          Length = 548

 Score =  444 bits (1143), Expect = e-124
 Identities = 234/558 (41%), Positives = 340/558 (60%), Gaps = 20/558 (3%)






 
Structural Features:
pfam02366, PMT, Dolichyl-phosphate-mannose-protein mannosyltransferase. 
CD-Length = 240 residues,  97.5% aligned
Score =  104 bits (261), Expect = 3e-23
Identities = 66/234 (28%), Positives = 94/234 (40%), Gaps = 5/234 (2%)

COG1807, ArnT, 4-amino-4-deoxy-L-arabinose transferase and related 
glycosyltransferases of PMT family 
[Cell envelope biogenesis, outer membrane]
CD-Length = 535 residues,  99.6% aligned
Score =  222 bits (566), Expect = 9e-59
Identities = 191/538 (35%), Positives = 253/538 (47%), Gaps = 24/538 (4%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1195




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18337




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32013




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:596_cdd.html


    		




 
URL:

















    ReferenceID:34145




  
Author/Investigator(s):
        Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR.





  
Title:
        An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor.




  
PubMed:
        11535604


MedLine:
        




    
Source:
J Biol Chem. 2001 Nov 16;276(46):43122-31. Epub 2001 Sep 04.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11535604









           
Homologs By Global Alignment 

           

       



     
Gene ID:596
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1019
                           PA3556_tr
                           translation of PA3556
                           549
                           99.08
                           99.45
                           0
                           2859.0
     

     
     
39808
                           gb|AAG06944.1|AE004776_7
                           (AE004776) inner membrane L-Ara4N transferase ArnT [Pseudomonas aeruginosa PAO1]
                           549
                           99.08
                           99.45
                           0
                           2859.0
     

     
     
39817
                           gb|AAC04774.1|
                           (AF036677) unknown [Salmonella typhimurium]
                           559
                           41.86
                           60.82
                           21
                           1168.0
     

     
     
39815
                           gb|AAL34393.1|
                           (AY057444) 4-amino-4-deoxy-L-arabinose lipid A transferase [Salmonella typhimurium]
                           559
                           41.86
                           60.82
                           21
                           1168.0
     

     
     
39812
                           gb|AAL21202.1|
                           (AE008803) putative melittin resistance protein [Salmonella typhimurium LT2]
                           559
                           41.86
                           60.82
                           21
                           1168.0
     

     






      

          
GC ORFID: 43598How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3556Glimmer Score: 

          
Start: 3380696Stop: 3382345

          
Length: 1650


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3556 translation of PA3556Homolog Bit Score 1105.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2587 (ORF: BLASTX 719.0), t_PA3556 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCGCCGCCAGACCTGGTCGCTGCTGCTGATCGCCTTCGGCCTGTTCTACCTGGTTCCATTGAGCAAC 
CATGGCCTGTGGATTCCCGACGAGACCCGCTACGCGCAGATCAGCCAGGCGATGCTGCtGGGCGGCGACTGG 
GTGTCGCCGCACTTCCTCGGCCTGCGCTACTTCGAGAAGCCGGTCGCCGGCTACTGGATGATCGCCCTCGGC 
CAGGCGGTCTTCGGCGAAAACCTGTTCGGCGTGCGCATCGCCTCGGTCGTCGCCACCGCCCTCAGCGTCCTG 
CTCGCCTACCTGCTGGCCCGCCGCCTGTGGCGCGACCCGCGCACCAGCCTGGCGTGCGCGCTGCTCTACGCC 
AGCTTCGGCTTGATCGCCGGGCAGTCCGGCTATGCCAACCTCGACCCGCAATTCACCTTCTGGGTCAACCTG 
AGCCTGGTGGCGCTGTGGTACGCCCTCGACGCGGGGAGCCGCCGCGCGCGCCTGCTCGGCTGGATCCTGCTC 
GGCCTCGCCTGCGGCATGGGCTTCCTGACCAAGGGCTTCCTCGCCTGGCTGCTGCCGGTGCTGGTCGCCCTG 
CCCTACATGCTCTGGCAGCGGCGCTGGCGCGAGCTGCTTGGCTACGGCGCGCTGGCGGTGCTGGCGGCGCTG 
CTCGTCTGCCTGCCCTGGGCGCTCGCCGTGCATGCGCGGGAAGCGGACTACTGGCGGTTCTTCTTCTGGCAC 
GAACACATCCGCCGCTTCGCCGGCGAAGACGCCCAGCACTCTCGCCCGTGGTGGTTCTACCTGCCGTTGCTG 
GTGGTCGCCTGCCTGCCCTGGAGCGGCCTGCTGCCGAGCGCTCTGCGCCAGGCCTGGCACGAGCGGCGCCAG 
GCGCCGGTGGTCTTCCTGGCGCTATGGCTGCTGTTGCCGCTGGCGTTCTTCAGCCTGAGCCGGGGCAAGCTG 
CCGACCTACATCATGCCCTGCCTGCTGCCGCTGGCACTGCTCATGGGCCACGCCCTGGTGCAGCGGCTGCGC 
CTGGGGAACAGCGTCGCGCTGCGCGGCAACGGGCTGCTCAACCTGGGCCTGGCGCTCCTCGCGCTGGCGGCC 
CTGGCCTACCTGCAACTGCGCAAGCCGGTGTACCAGGAAGAACCCTTCGAGCTGTTCCTGGTCCTGCTGGTG 
ATCGGCGCCTGGGCCGCCGCCGGCCTCGCCCAGTGGCGCTACCCGCTACGCGCCTGGGCCGCGCCGCTGCTG 
GCGAGCTGGGTGCTGATCGCGCTGCTGCCGGCAGCGATGCCCAACCATGTGGTGCAGAACAAGACCCCCGAC 
CTGTTCGTCGCCGAACACCTCGACGAACTGACCGGCGCCCGCCATCTGCTGAGCAACGACCTCGGCGCCGCC 
TCGGCCCTCGCCTGGCGCCTGCGTCGTAGCGACGTGACGCTCTACGACACGCGCGGCGAGCTGAAGTACGGC 
CTCTCCTACCCCGAGCACAGCCAGCGCAGCGTGCCCTTGGCAGACATCCGCCAGTGGCTCTGGCGGGCACGC 
CAGGACGGCTCGGTTGCCGTGCTGCTGCGGATCAACAGCGCCAGCGACCGCTACCAGCTGGCGCTGTTGCCG 
GGCGACGGCGAACGCTACCGGAACGGCAACCTGGTCCTGGCGATCCTGCCGCAGGTGCGCCCATGA