ViewGene
Locus Name:
GeneID:
Location:

GeneID:594
TrivialName: PA14_10850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10850
PAO1 Ortholog Locus:PA4100
Sequence Length:1680
Protein Length:559
Start: 940431
Stop: 938752
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07487.1|AE004826_5 (AE004826) probable dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 544/546 (99%), Positives = 544/546 (99%)
Sequence:
ATGACTTCCACTGCCCGCCGCTCGTTCGACTACATCGTGGTCGGCGCCGGCTCCGCCGGTTGCGTCCTGGCC
AACCGTCTTTCCGCCGATCCCGCCGTCTCGGTCTGCCTGGTCGAGGCCGGCCCCAGCGACCGCACGCCGCTC
CCCGCCGCCTACATCCGCACGCCGGCCGGGATCATCCGCCTGATCGCCAACCCGAAGTGGAACTGGATGCAT
CGCTTCGCCGCCCAGCCCGGCACCGCCAACCAGCCGATCGCCTGCCCGCGCGGCAAGGTCTGGGGCGGCTCC
AGCGCGATCAACGGGATGATCTACATCCGCGGCGACCGCCACGACTACGACCGCTGGGCATCCCTCGGCAAC
CGTGGCTGGAGCTACGACGAGCTGCTCCCCTACTTTCGCCACTCGGAACATTTCGAGCCGGGCGAATCGCCC
TGGCACGGGCGCGGCGGCGAATTGAACGTCGCCGCGCAGCGCAGCCCGGGGCCGATCAACCAGGTGTTCTTC
CAGGCCGCCGAGGAAATGGGCTGGCCGTACAACGCCGACTTCAACGGCGAGCGCCAGGAAGGCATCGGCCCG
TTCCACGTCACCCAGGTCAACGGCGAACGCTGTAGCGCGGCGCGCGCCTTCCTGCACCCGGCCCTGGCGCGG
CCCAACCTCACCGTCCTCAGCTCCGCGCTGACCCTGCGCGTGCTGCTGGAAGGCACCCGCGCCACCGGCGTG
GAGATCAGCCAGGCTGGCGAGGTCGTGCAATTGCAGGCACGCCGCGAAGTGATCCTCAGCGCCGGCTCGATC
AACTCGCCGCAGCTCCTGTTGCTCTCCGGCATCGGCCCGGCGGCGGAACTGGCGCGCCACGGCATCGTCCAG
CGCCATGAGTTGCCTGGGGTCGGCGAGAACCTGCAGGACCACCAGGACATCGTGCTGATGTACCGCACCGAG
GCCAAGCTCGGCTACGGCCTGGGTTTCTCGCCGAAGGGCTGGCTGCCGCTGCTGCGCTCGCCCTGGCAGTAC
CTGTTCGGCCGGCGCGGGGCGCTGACCTCGAACACCGTCGAGTCCGGTGGCTTCCTCCGCCTCGACCCGCAG
GCAGAGACCCCGGAGCTGGGTCTGATCGTCGCCCCCGCGCTGAAGAACCAGCCCCAGCGCCTGGTCCCCTTC
GGCCACGGGGTCAGCCTGCACGTCGCGGTGATGCACCCGCAGAGCCGCGGGCGGGTCCGCCTGAACTCGCCG
GACCCGCACGACCGGCCGCTGATCGAAGCCAACTTCCTCAGCCACCCTGCCGACCTCGATACCCTGGTCCAG
GGTTTCCAACTGGTCCGCAAACTGGCCGCCAGCCGCAGCTTCGCCCGTCACCTCAAGGGCGAGCTGGTACCG
GGCCCGCAGGTTTCCAGCCGCGGCCAGATCGAGGCCTGGATCCGCGCCAACCTGGGCACCGTGTTCCATCCG
GTGGGCACCTGCAAGATGGGCCACGACCAGCTCGCGGTGGTCGACGACCAGTTGCGCGTGCACGGCCTGCAA
GGCCTGCGGGTGGCCGATGCGTCGATCATGCCGACGCTGATCACCGGCAATACCAACGCCCCGGCGATCATG
ATCGGCGAGAAGGCCGCCGACCTGATCCTCGGCAAGCCGGCGCCCCATGCGCCGACGGAACAGCGGCGGGAT
GCGCCGGAGCTGGTAGAGGCCTGA
Translation:
MTSTARRSFDYIVVGAGSAGCVLANRLSADPAVSVCLVEAGPSDRTPLPAAYIRTPAGIIRLIANPKWNWMH
RFAAQPGTANQPIACPRGKVWGGSSAINGMIYIRGDRHDYDRWASLGNRGWSYDELLPYFRHSEHFEPGESP
WHGRGGELNVAAQRSPGPINQVFFQAAEEMGWPYNADFNGERQEGIGPFHVTQVNGERCSAARAFLHPALAR
PNLTVLSSALTLRVLLEGTRATGVEISQAGEVVQLQARREVILSAGSINSPQLLLLSGIGPAAELARHGIVQ
RHELPGVGENLQDHQDIVLMYRTEAKLGYGLGFSPKGWLPLLRSPWQYLFGRRGALTSNTVESGGFLRLDPQ
AETPELGLIVAPALKNQPQRLVPFGHGVSLHVAVMHPQSRGRVRLNSPDPHDRPLIEANFLSHPADLDTLVQ
GFQLVRKLAASRSFARHLKGELVPGPQVSSRGQIEAWIRANLGTVFHPVGTCKMGHDQLAVVDDQLRVHGLQ
GLRVADASIMPTLITGNTNAPAIMIGEKAADLILGKPAPHAPTEQRRDAPELVEA*
AnnotationID:603GeneID:594
AnnotatorUID: diggins
Modification Date/Time: 2005-06-08 06:06:06
Gene Name:
Confidence Code:3
GeneProduct:putative dehydrogenase
Cell Localization:()
Synonyms:PA4100
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:putative oxidoreductase
Functional Category Confidence Code:5
COGs:COG2303,pfam00732,pfam05199
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAC38030.1| (AJ295164) alcohol dehydrogenase [Alcanivorax borkumensis]
          Length = 535

 Score =  476 bits (1224), Expect = e-133
 Identities = 254/537 (47%), Positives = 342/537 (63%), Gaps = 11/537 (2%)

>emb|CAB51051.1| (AJ233397) alcohol dehydrogenase [Pseudomonas putida]
          Length = 552

 Score =  466 bits (1200), Expect = e-130
 Identities = 249/537 (46%), Positives = 335/537 (62%), Gaps = 13/537 (2%)

>gb|AAK24609.1| (AE005932) choline dehydrogenase [Caulobacter crescentus CB15]
          Length = 555

 Score =  443 bits (1140), Expect = e-123
 Identities = 248/549 (45%), Positives = 333/549 (60%), Gaps = 13/549 (2%)








 
Structural Features:
COG2303, BetA, Choline dehydrogenase and related flavoproteins 
[Amino acid transport and metabolism]
CD-Length = 542 residues,  99.4% aligned
Score =  498 bits (1282), Expect = 8e-142
Identities = 249/550 (45%), Positives = 319/550 (58%), Gaps = 19/550 (3%)

pfam00732, GMC_oxred_N, GMC oxidoreductase. 
This family of proteins bind FAD as a cofactor. 
CD-Length = 291 residues, 100.0% aligned
Score =  291 bits (747), Expect = 1e-79
Identities = 143/295 (48%), Positives = 188/295 (63%), Gaps = 11/295 (3%)

pfam05199, GMC_oxred_C, GMC oxidoreductase.
CD-Length = 202 residues, 100.0% aligned
Score =  198 bits (506), Expect = 1e-51
Identities = 94/202 (46%), Positives = 130/202 (64%), Gaps = 5/202 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1191




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18333




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36847




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:594_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:594
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1015
                           PA4100_tr
                           translation of PA4100
                           559
                           97.67
                           98.03
                           0
                           2862.0
     

     
     
39725
                           gb|AAG07487.1|AE004826_5
                           (AE004826) probable dehydrogenase [Pseudomonas aeruginosa PAO1]
                           559
                           97.67
                           98.03
                           0
                           2862.0
     

     
     
39726
                           dbj|BAC95059.1|
                           (AP005339) choline dehydrogenase [Vibrio vulnificus YJ016]
                           564
                           47.16
                           60.81
                           23
                           1230.0
     

     
     
39729
                           dbj|BAB50165.1|
                           (AP003001) dehydrogenase; polyethylene glycol dehydrogenase; alcohol dehydrogenase; L-sorbose dehydrogenase [Mesorhizobium loti]
                           566
                           46.28
                           58.65
                           38
                           1191.0
     

     
     
39727
                           emb|CAC38030.1|
                           (AJ295164) alcohol dehydrogenase [Alcanivorax borkumensis]
                           564
                           45.21
                           60.81
                           34
                           1231.0
     

     






      

          
GC ORFID: 43596How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4100Glimmer Score: 

          
Start: 4013506Stop: 4015185

          
Length: 1680


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4100 translation of PA4100Homolog Bit Score 1107.0

          
Other Homologs:  t_PA3710 (ORF: BLASTX 399.0), t_PA5372 (ORF: BLASTX 368.0), psyr_15may02_Scaffold6_revised_gene6626 (ORF: BLASTX 367.0), t_PA4100 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTTCCACTGCCCGCCGCTCGTTCGACTACATCGTGGTCGGCGCCGGCTCCGCCGGTTGCGTCCTGGCC 
AACCGTCTTTCCGCCGATCCCGCCGTCTCGGTCTGCCTGGTCGAGGCCGGCCCCAGCGACCGCACGCCGCTC 
CCCGCCGCCTACATCCGCACGCCGGCCGGGATCATCCGCCTGATCGCCAACCCGAAGTGGAACTGGATGCAT 
CGCTTCGCCGCCCAGCCCGGCACCGCCAACCAGCCGATCGCCTGCCCGCGCGGCAAGGTCTGGGGCGGCTCC 
AGCGCGATCAACGGGATGATCTACATCCGCGGCGACCGCCACGACTACGACCGCTGGGCATCCCTCGGCAAC 
CGTGGCTGGAGCTACGACGAGCTGCTCCCCTACTTTCGCCACTCGGAACATTTCGAGCCGGGCGAATCGCCC 
TGGCACGGGCGCGGCGGCGAATTGAACGTCGCCGCGCAGCGCAGCCCGGGGCCGATCAACCAGGTGTTCTTC 
CAGGCCGCCGAGGAAATGGGCTGGCCGTACAACGCCGACTTCAACGGCGAGCGCCAGGAAGGCATCGGCCCG 
TTCCACGTCACCCAGGTCAACGGCGAACGCTGTAGCGCGGCGCGCGCCTTCCTGCACCCGGCCCTGGCGCGG 
CCCAACCTCACCGTCCTCAGCTCCGCGCTGACCCTGCGCGTGCTGCTGGAAGGCACCCGCGCCACCGGCGTG 
GAGATCAGCCAGGCTGGCGAGGTCGTGCAATTGCAGGCACGCCGCGAAGTGATCCTCAGCGCCGGCTCGATC 
AACTCGCCGCAGCTCCTGTTGCTCTCCGGCATCGGCCCGGCGGCGGAACTGGCGCGCCACGGCATCGTCCAG 
CGCCATGAGTTGCCTGGGGTCGGCGAGAACCTGCAGGACCACCAGGACATCGTGCTGATGTACCGCACCGAG 
GCCAAGCTCGGCTACGGCCTGGGTTTCTCGCCGAAGGGCTGGCTGCCGCTGCTGCGCTCGCCCTGGCAGTAC 
CTGTTCGGCCGGCGCGGGGCGCTGACCTCGAACACCGTCGAGTCCGGTGGCTTCCTCCGCCTCGACCCGCAG 
GCAGAGACCCCGGAGCTGGGTCTGATCGTCGCCCCCGCGCTGAAGAACCAGCCCCAGCGCCTGGTCCCCTTC 
GGCCACGGGGTCAGCCTGCACGTCGCGGTGATGCACCCGCAGAGCCGCGGGCGGGTCCGCCTGAACTCGCCG 
GACCCGCACGACCGGCCGCTGATCGAAGCCAACTTCCTCAGCCACCCTGCCGACCTCGATACCCTGGTCCAG 
GGTTTCCAACTGGTCCGCAAACTGGCCGCCAGCCGCAGCTTCGCCCGTCACCTCAAGGGCGAGCTGGTACCG 
GGCCCGCAGGTTTCCAGCCGCGGCCAGATCGAGGCCTGGATCCGCGCCAACCTGGGCACCGTGTTCCATCCG 
GTGGGCACCTGCAAGATGGGCCACGACCAGCTCGCGGTGGTCGACGACCAGTTGCGCGTGCACGGCCTGCAA 
GGCCTGCGGGTGGCCGATGCGTCGATCATGCCGACGCTGATCACCGGCAATACCAACGCCCCGGCGATCATG 
ATCGGCGAGAAGGCCGCCGACCTGATCCTCGGCAAGCCGGCGCCCCATGCGCCGACGGAACAGCGGCGGGAT 
GCGCCGGAGCTGGTAGAGGCCTGA