ViewGene
Locus Name:
GeneID:
Location:

GeneID:5938
TrivialName: PA14_35900
AnnotatorUID: ericdeziel
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35900
PAO1 Ortholog Locus:
Sequence Length:921
Protein Length:306
Start: 3195183
Stop: 3196103
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAK01504.1| (AF241171) hypothetical dehydrogenase [Pseudomonas aeruginosa]
Identities = 301/306 (98%), Positives = 303/306 (99%) to gene #14 of PAGI-1 island.
Sequence:
GTGCGTGAACACAATGATGAAAGCGTCGAGTTCGAAGTGTCCGTGATCGGGCTCGGAGCCATGGGAACCATC
ATGGCCCAAGCGTTGCTCAGGCAAGGCAGGCGCGTGGCGATCTGGAACCGCTCACCCGGCAAGGCCGCGGCG
CTCGTCGCCGCTGGCGCTCACCTGTGCGAGAGCGCCGAAGCCGCGCTGGCTGCAAGCCCGGCCACGATCTTC
GTGCTCCTGGACAACCAGGCGACCCGCGAGGTGCTCGGGATGCCAGGTGTGATGCAGGCACTGGCCAACCGT
ACGATCGTCGATTACACGACCAACGCCCAGGACGAGGGTCTGGCGCTTCAGAGTCTCGTCAACCGCGCGGGC
GGCCATTACGTCAAAGGGATGATCGTGGCCTACCCGCGCAACGTTGGTCGTCGCGAAAGCCACAGCATTCAT
ACCGGTGATCCTGAGGCCTTCGAACGGCATCGGGCGCTGCTTGAGGGGCTTGCCGGTCATACGACGTTCCTC
CCCTGGGACGAAGCATTGGCCTTCGCCACCGTGCTCCACGCGCATGCGTTTGCCGCGATGGTGGCTTTCTTC
GAGGCCGTGGGGGCTGGTGTCCACTTCGGCCTGCCGCCTTCGAAGACCGCTCGGCTCATGCTCGACACGTCG
CGCTTTTTCGTCGCCGATGCACTCGAGGAAGCGGTCCGTCGGCTGGAAGCGCAGGATTTCGCAGGCGATCAG
GCGAGGCTCGATGTGCATGCCCAGGCCTTCGCCCATATCGCCGGAGCCCTGCACGCACAAGGGGCCTGGACG
CCGGTCTTCGACGCGCTGTGCCAGGCCGTGCAACGCGCGGCAGCCATGGGCTATGGCGATCAGGACATCGTA
GCCGTCACCCGCCTGTTCGCCCGCGAATGCGACACCGCAAGCGCGGCGGGACAGTGA
Translation:
MREHNDESVEFEVSVIGLGAMGTIMAQALLRQGRRVAIWNRSPGKAAALVAAGAHLCESAEAALAASPATIF
VLLDNQATREVLGMPGVMQALANRTIVDYTTNAQDEGLALQSLVNRAGGHYVKGMIVAYPRNVGRRESHSIH
TGDPEAFERHRALLEGLAGHTTFLPWDEALAFATVLHAHAFAAMVAFFEAVGAGVHFGLPPSKTARLMLDTS
RFFVADALEEAVRRLEAQDFAGDQARLDVHAQAFAHIAGALHAQGAWTPVFDALCQAVQRAAAMGYGDQDIV
AVTRLFARECDTASAAGQ*
AnnotationID:5939GeneID:5938
AnnotatorUID: danlee
Modification Date/Time: 2005-10-11 17:05:21
Gene Name:PAGI-1(14)
Confidence Code:3
GeneProduct:Putative dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|12698390|gb|AAK01504.1|  hypothetical dehydrogenase [Pseudomonas aeruginosa]
     Length=319
     Score =  594 bits (1532),  Expect = 1e-168
     Identities = 301/306 (98%), Positives = 303/306 (99%), Gaps = 0/306 (0%)

gi|24984390|gb|AAN68406.1|  oxidoreductase, putative [Pseudomonas put ida KT2440]
     Length=302
     Score =  516 bits (1330),  Expect = 3e-145
     Identities = 259/297 (87%), Positives = 274/297 (92%), Gaps = 0/297 (0%)








 
Structural Features:
COG2084, MmsB, 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid 
dehydrogenases [Lipid metabolism].
             CD-Length = 286 residues,  99.3% aligned
             Score = 66.0 bits (161), Expect = 6e-12






   
Genomic Context:
Predicted gene #X of the PAGI-1 island absent in PAO1 but present in a majority of pathogenic P. aeruginosa isolates.   
The PA14 island at this locus, from PA14_35860 to PA14_36030, corresponds to PAGI-1 genes 17 through 2, respectively.







   
Comment:
The first half of the PA14 island (from PA14_35700 through PA14_35850) diverges from PAGI-1 such that 
genes 18 through 51 are absent.  PA14 has 31 ORFs in 35368 bp (from PA14_35700 to PA14_36030), 
whereas PAGI-1 contains 51 ORFs in 51300 bp.


















    ReferenceID:15788




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:28996




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38204




  
Author/Investigator(s):
        Liang X, Pham XQ, Olson MV, Lory S.





  
Title:
        Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa.




  
PubMed:
        11208781


MedLine:
        




    
Source:
J Bacteriol. 2001 Feb;183(3):843-53.







    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:5938
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
197965
                           gb|AAK01504.1|
                           (AF241171) hypothetical dehydrogenase [Pseudomonas aeruginosa]
                           319
                           94.67
                           94.98
                           13
                           1535.0
     

     
     
197966
                           gb|AAN68406.1|AE016473_4
                           (AE016784) oxidoreductase, putative [Pseudomonas putida KT2440]
                           306
                           84.31
                           89.86
                           4
                           1323.0
     

     






      

          
GC ORFID: 48940How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 1758733Stop: 1757813

          
Length: 921


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: Homolog Bit Score 

          
Other Homologs:  

          
GC ORF Sequence

           
GTGCGTGAACACAATGATGAAAGCGTCGAGTTCGAAGTGTCCGTGATCGGGCTCGGAGCCATGGGAACCATC 
ATGGCCCAAGCGTTGCTCAGGCAAGGCAGGCGCGTGGCGATCTGGAACCGCTCACCCGGCAAGGCCGCGGCG 
CTCGTCGCCGCTGGCGCTCACCTGTGCGAGAGCGCCGAAGCCGCGCTGGCTGCAAGCCCGGCCACGATCTTC 
GTGCTCCTGGACAACCAGGCGACCCGCGAGGTGCTCGGGATGCCAGGTGTGATGCAGGCACTGGCCAACCGT 
ACGATCGTCGATTACACGACCAACGCCCAGGACGAGGGTCTGGCGCTTCAGAGTCTCGTCAACCGCGCGGGC 
GGCCATTACGTCAAAGGGATGATCGTGGCCTACCCGCGCAACGTTGGTCGTCGCGAAAGCCACAGCATTCAT 
ACCGGTGATCCTGAGGCCTTCGAACGGCATCGGGCGCTGCTTGAGGGGCTTGCCGGTCATACGACGTTCCTC 
CCCTGGGACGAAGCATTGGCCTTCGCCACCGTGCTCCACGCGCATGCGTTTGCCGCGATGGTGGCTTTCTTC 
GAGGCCGTGGGGGCTGGTGTCCACTTCGGCCTGCCGCCTTCGAAGACCGCTCGGCTCATGCTCGACACGTCG 
CGCTTTTTCGTCGCCGATGCACTCGAGGAAGCGGTCCGTCGGCTGGAAGCGCAGGATTTCGCAGGCGATCAG 
GCGAGGCTCGATGTGCATGCCCAGGCCTTCGCCCATATCGCCGGAGCCCTGCACGCACAAGGGGCCTGGACG 
CCGGTCTTCGACGCGCTGTGCCAGGCCGTGCAACGCGCGGCAGCCATGGGCTATGGCGATCAGGACATCGTA 
GCCGTCACCCGCCTGTTCGCCCGCGAATGCGACACCGCAAGCGCGGCGGGACAGTGA