ViewGene
Locus Name:
GeneID:
Location:

GeneID:592
TrivialName: PA14_02550
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02550
PAO1 Ortholog Locus:PA0208
Sequence Length:1665
Protein Length:554
Start: 227383
Stop: 229047
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03597.1|AE004459_1 (AE004459) malonate decarboxylase alpha subunit [Pseudomonas aeruginosa PAO1]
Identities = 552/554 (99%), Positives = 552/554 (99%)
Sequence:
ATGACGACACCGATCTCTCCCCCGCCGCAGTGGTCGCGGCGCCGCCAGGAAAAGCAGCGGCGCCTCGAGCGC
GTGCGCGGCCTGGCCGACGGCGCGGTGCTGCCGCGCGAACGCCTGGTCGCCGCGCTGGAGGCGCTGATCGCG
CCGGGCGATCGGGTGGTGCTGGAAGGCAACAACCAGAAGCAGGCGGACTTCCTCTCCCGTTCGCTGGCGCGG
GTCGATCCCGGCAAGCTGCACGACCTGCACATGATCATGCCCAGCGTCGGTCGTCCGGAGCATCTCGACCTG
TTCGAACTGGGCATCGCGCGCAAGCTCGACTTCTCCTTCTCCGGCCCGCAGAGCCTGCGCATCGGCCAGTTG
CTCGAAGACGGCCTGCTGGAGATCGGCGCGATCCACACCTACATCGAGCTGTACGCGCGGCTGGTGGTCGAC
CTGATCCCCAACGTCGCACTGGTCGCCGGTTTCGTCGCCGACCGCGAAGGCAACGTGTACACCGGGCCGAGC
ACCGAAGACACCCCGGCGCTGGTGGAGCCCACCGCGTTCAGCGACGGCATCGTCATCGTCCAGGTCAACCGT
ATCGTCGACGACCCGCGCGACCTGCCGCGAGTGGACATCCCGGCGTCCTGGGTGGACTTCGTGGTCGAGGCC
GACCAGCCGTTCTACATCGAGCCGCTGTTCACCCGCGACCCGCGCCACATCAAGCCGGTCCACGTGCTGATG
GCGATGATGGCGATCCGCGGCATCTACCAGCGGCACAACGTGCAGTCGCTGAACCACGGCATCGGCTTCAAC
ACCGCGGCCATCGAGCTGATCCTGCCGACCTACGGCGAGTCCCTCGGCCTGAAGGGCAAGATCTGCCGGCAC
TGGACCCTCAACCCGCACCCGACCCTTATCCCCGCCATCGAGAGCGGCTGGGTCGAGAGCGTGCACTGCTTC
GGCACCGAGCTGGGCATGGAGGGCTACATCGCCCAGCGTCCCGACGTGTTCTTCACCGGTCGCGACGGCTCG
CTGCGTTCCAATCGGATGTTCTGCCAACTGGCCGGGCAGTACGCGGTAGACCTGTTCATCGGCGCCACCCTC
CAGGTCGACGGCGACGGCCATTCCTCCACCGTCACCCGCGGACGCCTGGCCGGCTTCGGCGGCGCCCCGAAC
ATGGGCCACGACCCGCGCGGCCGGCGCCACTCGACGGCGGCCTGGCTGGACATGCGGGGCGAACCCGAGGCC
TTGCTGGAGCGCGGCAGGAAGCTGGTGGTGCAGATGGTCGAGACCTTCCAGGACGGCGGCAAGCCGACCTTC
GTCGAGCGTCTCGACGCGCTGGAGGTGGCGCGCCAGACCGGCATGCCGCTGGCGCCGGTGATGATCTATGGC
GACGACGTCACCCATGTGCTCACCGAGGAAGGCATCGCCTACCTGTACAAGGCGCGCTCGCTGGAGGAACGC
CAGGCGATGATCGCCGCGGTGGCGGGGATCAGTCCGATCGGCCTGCGCCACGATCCGCGCGAGACCCAGCGG
ATGCGCCGCGAAGGGCTGATCGCGCTGCCCGAGGACCTCGGCATCCGCCGCACCGACGCCTCGCGCGAGCTG
CTGGCGGCGAAGAGCATCGCCGAGCTGGTCGAGTGGTCCGGCGGGCTGTACCAGCCGCCGGCGCGTTTCAGG
AGCTGGTGA
Translation:
MTTPISPPPQWSRRRQEKQRRLERVRGLADGAVLPRERLVAALEALIAPGDRVVLEGNNQKQADFLSRSLAR
VDPGKLHDLHMIMPSVGRPEHLDLFELGIARKLDFSFSGPQSLRIGQLLEDGLLEIGAIHTYIELYARLVVD
LIPNVALVAGFVADREGNVYTGPSTEDTPALVEPTAFSDGIVIVQVNRIVDDPRDLPRVDIPASWVDFVVEA
DQPFYIEPLFTRDPRHIKPVHVLMAMMAIRGIYQRHNVQSLNHGIGFNTAAIELILPTYGESLGLKGKICRH
WTLNPHPTLIPAIESGWVESVHCFGTELGMEGYIAQRPDVFFTGRDGSLRSNRMFCQLAGQYAVDLFIGATL
QVDGDGHSSTVTRGRLAGFGGAPNMGHDPRGRRHSTAAWLDMRGEPEALLERGRKLVVQMVETFQDGGKPTF
VERLDALEVARQTGMPLAPVMIYGDDVTHVLTEEGIAYLYKARSLEERQAMIAAVAGISPIGLRHDPRETQR
MRREGLIALPEDLGIRRTDASRELLAAKSIAELVEWSGGLYQPPARFRSW*
AnnotationID:601GeneID:592
AnnotatorUID: diggins
Modification Date/Time: 2004-08-01 17:05:05
Gene Name:mdcA
Confidence Code:2
GeneProduct: malonate decarboxylase alpha subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA36204.1| (AB017138) alpha subunit of malonate decarboxylase [Pseudomonas
           putida]
          Length = 553

 Score =  983 bits (2541), Expect = 0.0
 Identities = 480/554 (86%), Positives = 521/554 (94%), Gaps = 1/554 (0%)

>gb|AAO58514.1| (AE016874) malonate decarboxylase, alpha subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 558

 Score =  978 bits (2528), Expect = 0.0
 Identities = 480/558 (86%), Positives = 520/558 (93%), Gaps = 4/558 (0%)

>gb|AAC45453.1| (U95087) MdcA [Klebsiella pneumoniae]
          Length = 551

 Score =  849 bits (2194), Expect = 0.0
 Identities = 420/544 (77%), Positives = 469/544 (86%), Gaps = 3/544 (0%)






 
Structural Features:






   
Genomic Context:






   
Comment:


















    ReferenceID:1187




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18329




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33573




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:592_cdd.html


    		




 
URL:

















    ReferenceID:35468




  
Author/Investigator(s):
        Chohnan S, Kurusu Y, Nishihara H, Takamura Y.





  
Title:
        Cloning and characterization of mdc genes encoding malonate decarboxylase from Pseudomonas putida.




  
PubMed:
        10339824


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 May 15;174(2):311-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10339824

















    ReferenceID:35472




  
Author/Investigator(s):
        Koo JH, Jung SB, Byun HS, Kim YS.





  
Title:
        Cloning and sequencing of genes encoding malonate decarboxylase in Acinetobacter calcoaceticus.




  
PubMed:
        9375791


MedLine:
        




    
Source:
Biochim Biophys Acta. 1997 Oct 9;1354(1):49-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9375791









           
Homologs By Global Alignment 

           

       



     
Gene ID:592
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1013
                           PA0208_tr
                           translation of PA0208
                           554
                           99.63
                           99.63
                           0
                           2865.0
     

     
     
39712
                           gb|AAG03597.1|AE004459_1
                           (AE004459) malonate decarboxylase alpha subunit [Pseudomonas aeruginosa PAO1]
                           554
                           99.63
                           99.63
                           0
                           2865.0
     

     
     
39713
                           dbj|BAA36204.1|
                           (AB017138) alpha subunit of malonate decarboxylase [Pseudomonas putida]
                           554
                           86.64
                           94.04
                           1
                           2543.0
     

     
     
39714
                           gb|AAO58514.1|
                           (AE016874) malonate decarboxylase, alpha subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           558
                           86.02
                           93.18
                           4
                           2531.0
     

     
     
39715
                           gb|AAC45453.1|
                           (U95087) MdcA [Klebsiella pneumoniae]
                           554
                           75.99
                           85.01
                           3
                           2193.0
     

     






      

          
GC ORFID: 43594How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0208Glimmer Score: 

          
Start: 6028724Stop: 6030388

          
Length: 1665


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0208 translation of PA0208Homolog Bit Score 1108.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3317 (ORF: BLASTX 979.0), t_PA0208 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGACACCGATCTCTCCCCCGCCGCAGTGGTCGCGGCGCCGCCAGGAAAAGCAGCGGCGCCTCGAGCGC 
GTGCGCGGCCTGGCCGACGGCGCGGTGCTGCCGCGCGAACGCCTGGTCGCCGCGCTGGAGGCGCTGATCGCG 
CCGGGCGATCGGGTGGTGCTGGAAGGCAACAACCAGAAGCAGGCGGACTTCCTCTCCCGTTCGCTGGCGCGG 
GTCGATCCCGGCAAGCTGCACGACCTGCACATGATCATGCCCAGCGTCGGTCGTCCGGAGCATCTCGACCTG 
TTCGAACTGGGCATCGCGCGCAAGCTCGACTTCTCCTTCTCCGGCCCGCAGAGCCTGCGCATCGGCCAGTTG 
CTCGAAGACGGCCTGCTGGAGATCGGCGCGATCCACACCTACATCGAGCTGTACGCGCGGCTGGTGGTCGAC 
CTGATCCCCAACGTCGCACTGGTCGCCGGTTTCGTCGCCGACCGCGAAGGCAACGTGTACACCGGGCCGAGC 
ACCGAAGACACCCCGGCGCTGGTGGAGCCCACCGCGTTCAGCGACGGCATCGTCATCGTCCAGGTCAACCGT 
ATCGTCGACGACCCGCGCGACCTGCCGCGAGTGGACATCCCGGCGTCCTGGGTGGACTTCGTGGTCGAGGCC 
GACCAGCCGTTCTACATCGAGCCGCTGTTCACCCGCGACCCGCGCCACATCAAGCCGGTCCACGTGCTGATG 
GCGATGATGGCGATCCGCGGCATCTACCAGCGGCACAACGTGCAGTCGCTGAACCACGGCATCGGCTTCAAC 
ACCGCGGCCATCGAGCTGATCCTGCCGACCTACGGCGAGTCCCTCGGCCTGAAGGGCAAGATCTGCCGGCAC 
TGGACCCTCAACCCGCACCCGACCCTTATCCCCGCCATCGAGAGCGGCTGGGTCGAGAGCGTGCACTGCTTC 
GGCACCGAGCTGGGCATGGAGGGCTACATCGCCCAGCGTCCCGACGTGTTCTTCACCGGTCGCGACGGCTCG 
CTGCGTTCCAATCGGATGTTCTGCCAACTGGCCGGGCAGTACGCGGTAGACCTGTTCATCGGCGCCACCCTC 
CAGGTCGACGGCGACGGCCATTCCTCCACCGTCACCCGCGGACGCCTGGCCGGCTTCGGCGGCGCCCCGAAC 
ATGGGCCACGACCCGCGCGGCCGGCGCCACTCGACGGCGGCCTGGCTGGACATGCGGGGCGAACCCGAGGCC 
TTGCTGGAGCGCGGCAGGAAGCTGGTGGTGCAGATGGTCGAGACCTTCCAGGACGGCGGCAAGCCGACCTTC 
GTCGAGCGTCTCGACGCGCTGGAGGTGGCGCGCCAGACCGGCATGCCGCTGGCGCCGGTGATGATCTATGGC 
GACGACGTCACCCATGTGCTCACCGAGGAAGGCATCGCCTACCTGTACAAGGCGCGCTCGCTGGAGGAACGC 
CAGGCGATGATCGCCGCGGTGGCGGGGATCAGTCCGATCGGCCTGCGCCACGATCCGCGCGAGACCCAGCGG 
ATGCGCCGCGAAGGGCTGATCGCGCTGCCCGAGGACCTCGGCATCCGCCGCACCGACGCCTCGCGCGAGCTG 
CTGGCGGCGAAGAGCATCGCCGAGCTGGTCGAGTGGTCCGGCGGGCTGTACCAGCCGCCGGCGCGTTTCAGG 
AGCTGGTGA