ViewGene
Locus Name:
GeneID:
Location:

GeneID:584
TrivialName: PA14_33450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_33450
PAO1 Ortholog Locus:PA2416
Sequence Length:1638
Protein Length:545
Start: 2945267
Stop: 2943630
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05804.1|AE004669_1 (AE004669) periplasmic trehalase precursor [Pseudomonas aeruginosa PAO1]
Identities = 542/545 (99%), Positives = 543/545 (99%)
Sequence:
ATGCCTGATCGAACCGCTCTACCCCGCGCCATGCTCGCGGCCTGGGTCCTGCTCCTGCTGGCGGCCTGCAGC
CAGGGCCCGGCGCCTACCCCGCCCGCGTCCTGGGGTTGGCAGGACGCCAGCGGCGAACGCGCGATCGCCCCC
GACGAAGCCTATCCCGAGCTGTTCCAGGCGGTGCAGGAGAGCCGGCTGTTCAGCGACCAGAAACACTTCGTC
GATGCGCTCCCGCTGCGCGAGCCTGCACGGATCCGCGCCGACTACCTGCGCGAGAGGGAGCGGCCCGGCTTC
GACCTGCGCGCCTTCGTCGGCCGCAACTTCGAGGAGTCCGGCAGCGTCGAGACCGCCCCGCCGGAAGCGGGC
GCCGATCTCGCCAGCCACATCAGCGACCTGTGGCCGGCGTTGACCCGCCACTACGAGCAGGTGCCGGCCCAC
AGCAGCCTGCTGCCGCTGCCGAAACCCTACGTGGTCCCCGGCGGACGCTTCCGCGAGGTGTACTACTGGGAC
TCCTACTTCACCATGCTCGGCCTTGCGGAGAGCGGGCAGCACCAGCGGGTGCGCGACATGCTCGACAACTTC
GCCTACCTGATCGATACCTACGGGCACATTCCCAACGGTAACCGCAGCTACTACCTGAGCCGTTCGCAGCCG
CCGTTCTTCGCCTACATGGTGGACCTCCAGGCAAGACGCGAAGGCGACGCCGCCTACCGGCGCTACCTGCCG
CAATTACAGAAGGAATACGCCTACTGGATGGAAGGCTCCGCCGGGCTGCGCCACAACGAGGCGCGCCTGCAC
GTGGTGAAACTGGCCGACGGCAGCCTGCTGAACCGCTACTGGGACAACCGTGACACACCGCGCCAGGAGTCC
TTCCTGGAGGACCGGGCGACCGCCGCGCGCGCCCCGCAGCGCCCCGCGGGCGAGGTCTATCGCGACCTGCGG
GCCGGCGCGGAAAGCGGCTGGGACTTCTCCTCCCGCTGGCTGGACGACGGCCGCGAACTAGCCAGCATCCGC
ACCACCGCCATCGTCCCGGTGGACCTCAACGCCCTGCTCTACCATCTCGAACGAACCATCGCCAAGGCCTGC
GCCAGCAGCGCGCTGAAGGCCTGCGAGCAAGGCTACGGAGCCCGCGCGGAGAAGCGACGACAGGCCATCGAA
GACCACCTCTGGCACCCGGCGGGCTACTACGCCGACTACGACTGGCAACGCCGGCGGCCCATCGAGCGGATC
AACGCCGCCAGCCTCTTCCCGCTATTCACCGGGCTGGCCTCCGCCGAACGCGCCGGTCGTACCGCCGACAGC
GTCGCCGCCCAACTGCTGCGCCCGGGCGGACTGGCCACCACCACCCGTGCCAGCGGCCAGCAATGGGACGAG
CCGAACGGCTGGGCACCCTTGCAGTGGGTCGCCGTACAGGGCCTGCGCGCCTACGGCCGGGACGCCCTGGCG
GAAGACATCGGCCGGCGATTCCTCGCCCAGGTGCAGCAGGTCTACGACCGCGAAGGAAAACTGGTGGAGAAA
TACGACATCAGCGGCAACCAGGGCGGCGGCGGCGGTGGCGAGTACCCGCTGCAGGACGGCTTCGGCTGGTCC
AACGGCGTGACCCTGCAACTGCTCCGACTCTACGGCCCGGGCGCCGGTCGCTGA
Translation:
MPDRTALPRAMLAAWVLLLLAACSQGPAPTPPASWGWQDASGERAIAPDEAYPELFQAVQESRLFSDQKHFV
DALPLREPARIRADYLRERERPGFDLRAFVGRNFEESGSVETAPPEAGADLASHISDLWPALTRHYEQVPAH
SSLLPLPKPYVVPGGRFREVYYWDSYFTMLGLAESGQHQRVRDMLDNFAYLIDTYGHIPNGNRSYYLSRSQP
PFFAYMVDLQARREGDAAYRRYLPQLQKEYAYWMEGSAGLRHNEARLHVVKLADGSLLNRYWDNRDTPRQES
FLEDRATAARAPQRPAGEVYRDLRAGAESGWDFSSRWLDDGRELASIRTTAIVPVDLNALLYHLERTIAKAC
ASSALKACEQGYGARAEKRRQAIEDHLWHPAGYYADYDWQRRRPIERINAASLFPLFTGLASAERAGRTADS
VAAQLLRPGGLATTTRASGQQWDEPNGWAPLQWVAVQGLRAYGRDALAEDIGRRFLAQVQQVYDREGKLVEK
YDISGNQGGGGGGEYPLQDGFGWSNGVTLQLLRLYGPGAGR*
AnnotationID:593GeneID:584
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 08:08:03
Gene Name:treA
Confidence Code:2
GeneProduct:periplasmic trehalase precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1626,pfam01204
Secondary Functional Category(ies):
EC Number:3.2.1.28
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM42799.1| (AE012473) periplasmic trehalase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 568

 Score =  701 bits (1809), Expect = 0.0
 Identities = 346/521 (66%), Positives = 391/521 (75%), Gaps = 15/521 (2%)

>gb|AAL20711.1| (AE008780) trehalase, periplasmic [Salmonella typhimurium LT2]
          Length = 570

 Score =  594 bits (1532), Expect = e-169
 Identities = 294/495 (59%), Positives = 359/495 (72%), Gaps = 1/495 (0%)

>gb|AAN80119.1|AE016759_393 (AE016759) Periplasmic trehalase precursor [Escherichia coli
           CFT073]
          Length = 565

 Score =  583 bits (1504), Expect = e-166
 Identities = 287/495 (57%), Positives = 352/495 (71%), Gaps = 1/495 (0%)








 
Structural Features:
COG1626, TreA, Neutral trehalase [Carbohydrate transport and metabolism]
CD-Length = 558 residues,  99.8% aligned
Score =  659 bits (1701), Expect = 0.0
Identities = 306/557 (54%), Positives = 363/557 (65%), Gaps = 12/557 (2%)

pfam01204, Trehalase, Trehalase
CD-Length = 553 residues,  92.4% aligned
Score =  490 bits (1263), Expect = 2e-139
Identities = 221/516 (42%), Positives = 302/516 (58%), Gaps = 24/516 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1171




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18313




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32957




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:584_cdd.html


    		




 
URL:

















    ReferenceID:34492




  
Author/Investigator(s):
        Gutierrez C, Ardourel M, Bremer E, Middendorf A, Boos W, Ehmann U.





  
Title:
        Analysis and DNA sequence of the osmoregulated treA gene encoding the periplasmic trehalase of Escherichia coli K12.




  
PubMed:
        2671658


MedLine:
        




    
Source:
Mol Gen Genet. 1989 Jun;217(2-3):347-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2671658

















    ReferenceID:34493




  
Author/Investigator(s):
        Yamamoto H, Uchiyama S, Sekiguchi J.





  
Title:
        Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees region of the Bacillus subtilis chromosome containing genes for trehalose metabolism and acetoin utilization.




  
PubMed:
        8969503


MedLine:
        




    
Source:
Microbiology. 1996 Nov;142 ( Pt 11):3057-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8969503

















    ReferenceID:34494




  
Author/Investigator(s):
        Matthijs S, Koedam N, Cornelis P, De Greve H.





  
Title:
        The trehalose operon of Pseudomonas fluorescens ATCC 17400.




  
PubMed:
        11191810


MedLine:
        




    
Source:
Res Microbiol. 2000 Dec;151(10):845-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11191810









           
Homologs By Global Alignment 

           

       



     
Gene ID:584
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1003
                           PA2416_tr
                           translation of PA2416
                           545
                           99.44
                           99.63
                           0
                           2883.0
     

     
     
39391
                           gb|AAG05804.1|AE004669_1
                           (AE004669) periplasmic trehalase precursor [Pseudomonas aeruginosa PAO1]
                           545
                           99.44
                           99.63
                           0
                           2883.0
     

     
     
39392
                           gb|AAM42799.1|
                           (AE012473) periplasmic trehalase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           584
                           60.44
                           69.34
                           55
                           1817.0
     

     
     
39393
                           gb|AAM35493.1|
                           (AE011688) trehalase, periplasmic [Xanthomonas axonopodis pv. citri str. 306]
                           587
                           59.96
                           67.97
                           61
                           1800.0
     

     
     
39394
                           emb|CAD17428.1|
                           (AL646077) PROBABLE PERIPLASMIC ALPHA,ALPHA-TREHALASE SIGNAL PEPTIDE PROTEIN [Ralstonia solanacearum]
                           567
                           53.26
                           66.13
                           38
                           1542.0
     

     






      

          
GC ORFID: 43586How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2416Glimmer Score: 

          
Start: 2008649Stop: 2010286

          
Length: 1638


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2416 translation of PA2416Homolog Bit Score 1115.0

          
Other Homologs:  t_PA2416 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCTGATCGAACCGCTCTACCCCGCGCCATGCTCGCGGCCTGGGTCCTGCTCCTGCTGGCGGCCTGCAGC 
CAGGGCCCGGCGCCTACCCCGCCCGCGTCCTGGGGTTGGCAGGACGCCAGCGGCGAACGCGCGATCGCCCCC 
GACGAAGCCTATCCCGAGCTGTTCCAGGCGGTGCAGGAGAGCCGGCTGTTCAGCGACCAGAAACACTTCGTC 
GATGCGCTCCCGCTGCGCGAGCCTGCACGGATCCGCGCCGACTACCTGCGCGAGAGGGAGCGGCCCGGCTTC 
GACCTGCGCGCCTTCGTCGGCCGCAACTTCGAGGAGTCCGGCAGCGTCGAGACCGCCCCGCCGGAAGCGGGC 
GCCGATCTCGCCAGCCACATCAGCGACCTGTGGCCGGCGTTGACCCGCCACTACGAGCAGGTGCCGGCCCAC 
AGCAGCCTGCTGCCGCTGCCGAAACCCTACGTGGTCCCCGGCGGACGCTTCCGCGAGGTGTACTACTGGGAC 
TCCTACTTCACCATGCTCGGCCTTGCGGAGAGCGGGCAGCACCAGCGGGTGCGCGACATGCTCGACAACTTC 
GCCTACCTGATCGATACCTACGGGCACATTCCCAACGGTAACCGCAGCTACTACCTGAGCCGTTCGCAGCCG 
CCGTTCTTCGCCTACATGGTGGACCTCCAGGCAAGACGCGAAGGCGACGCCGCCTACCGGCGCTACCTGCCG 
CAATTACAGAAGGAATACGCCTACTGGATGGAAGGCTCCGCCGGGCTGCGCCACAACGAGGCGCGCCTGCAC 
GTGGTGAAACTGGCCGACGGCAGCCTGCTGAACCGCTACTGGGACAACCGTGACACACCGCGCCAGGAGTCC 
TTCCTGGAGGACCGGGCGACCGCCGCGCGCGCCCCGCAGCGCCCCGCGGGCGAGGTCTATCGCGACCTGCGG 
GCCGGCGCGGAAAGCGGCTGGGACTTCTCCTCCCGCTGGCTGGACGACGGCCGCGAACTAGCCAGCATCCGC 
ACCACCGCCATCGTCCCGGTGGACCTCAACGCCCTGCTCTACCATCTCGAACGAACCATCGCCAAGGCCTGC 
GCCAGCAGCGCGCTGAAGGCCTGCGAGCAAGGCTACGGAGCCCGCGCGGAGAAGCGACGACAGGCCATCGAA 
GACCACCTCTGGCACCCGGCGGGCTACTACGCCGACTACGACTGGCAACGCCGGCGGCCCATCGAGCGGATC 
AACGCCGCCAGCCTCTTCCCGCTATTCACCGGGCTGGCCTCCGCCGAACGCGCCGGTCGTACCGCCGACAGC 
GTCGCCGCCCAACTGCTGCGCCCGGGCGGACTGGCCACCACCACCCGTGCCAGCGGCCAGCAATGGGACGAG 
CCGAACGGCTGGGCACCCTTGCAGTGGGTCGCCGTACAGGGCCTGCGCGCCTACGGCCGGGACGCCCTGGCG 
GAAGACATCGGCCGGCGATTCCTCGCCCAGGTGCAGCAGGTCTACGACCGCGAAGGAAAACTGGTGGAGAAA 
TACGACATCAGCGGCAACCAGGGCGGCGGCGGCGGTGGCGAGTACCCGCTGCAGGACGGCTTCGGCTGGTCC 
AACGGCGTGACCCTGCAACTGCTCCGACTCTACGGCCCGGGCGCCGGTCGCTGA