ViewGene
Locus Name:
GeneID:
Location:

GeneID:582
TrivialName: PA14_09660
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_09660
PAO1 Ortholog Locus:PA4198
Sequence Length:1623
Protein Length:540
Start: 830659
Stop: 829037
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07585.1|AE004836_4 (AE004836) probable AMP-binding enzyme [Pseudomonas aeruginosa PAO1]
Identities = 539/540 (99%), Positives = 540/540 (100%)
Sequence:
ATGTCGATCTTCGAACAGGGTCTCGCGCCCGCCGCCGTCAACCACATCGCCCTGACGCCGCTGAGCTTCATC
GAGCGCACCGCCAGCGTCTATCCGCACTACCCCGCGGTGATCCACGGCAACATCCGCCGCGACTGGGCGCAG
ACCTACCAACGCTGCCGACGCCTGGCCTCGGCCCTGGCCGGGCGCGGCATCGGCCAGGGCGACACGGTGGCG
GTGATGCTGCCGAACATCCCCGAGATGCTCGAGGCGCACTATGGCGTGCCGATGATCGGCGCGGTGCTGAAC
ACCCTCAACGTACGCCTCGACGCCGAAGCCATCGCCTTCATGCTGCAACACGGCGAAGCCAAGGTGCTGATC
ACCGACTACGAATTCCACGAGGTGATCCATGCCGCCATCGGCATGCTCGACCACCCGCCGCTGGTGGTCGAC
GTCAACGACCCCGAGTATGGCGAAGGCCGCCCGGTTGGCGAACTGGACTACGAGGCGCTGCTCGCCGAGGGC
GACCCGCAGTTCGCCTGGGAATGGCCCCGGGACGAGTGGCAGGCGATCTCGCTGAACTACACCTCGGGCACC
ACCGGCAACCCCAAGGGCGTGGTCTACCATCACCGCGGCGCCTACCTGAACGCGCTGGGCAACCAGATGACC
TGGTCCATGGGCCAGCACCCGGTGTACCTCTGGACCCTGCCGATGTTCCACTGCAACGGCTGGTGCTATCCG
TGGACGGTGACCGCGCTGGCCGGCGTCCACGTCTGCCTGCGGCGGGTCGATCCGCAGAAGGTCCTCACCCTG
ATCCGCGAGCACCAGGTCAGCCACCTGTGCGGCGCACCCATCGTGCTCAACGCCCTGATCAACATGCCCGAC
TCGGCCAAGGCCGCCATCGACCACCCGGTGCATGCCATGGTCGCCGGCGCCGCGCCACCGGCCAAGGTGATC
GGCGCGGTGGAAGAGATGGGCATCCGCGTCACCCATGTCTACGGCCTCACCGAGGTCTACGGCCCGGTCACG
GTCTGCGCCTGGCACGGCGAGTGGGACGAACTGCCGCTGGAGCGGCGCGCCGCGATCAAGTCGCGCCAGGGC
GTGCGCTACCCGACCCTCGAAGGGGTGATGGTGGCCGACCCGAAGACCCTCGAGCCGGTGCCGCGCGACGGC
CAGTCCATCGGCGAGATCTTCATGCGCGGCAATACCGTGATGAAGGGCTACCTGAAGAATCCCAGCGCCACC
GAGGAAGCCTTCGCCGGCGGCTGGTTCCATACCGGCGACCTGGCGGTCTGCCATCCGGACGGCTACATCGAG
ATCCGCGACCGGCTCAAGGACATCATCATCTCCGGCGGCGAAAACATCTCCACCATCGAACTGGAGGGCGTG
CTCTACCGCCACCCGGCGGTGCTGGAGGCGGCGGTGGTGGCGCGGCCCGACGAAAAGTGGGGGGAAACGCCC
TGTGCGTTCATTACCCTGAAGAGCGATCATCAGGGGCTCGCCGAGAGCGAGATCGTCGCGTTCTGTCGCGAA
CACCTGGCCGCGTTCAAGATTCCGCGTACCGTGGTGTTCTCGGAACTGCCGAAGACCTCCACCGGCAAGATC
CAGAAATACGTGCTGCGCGAGTGGGCCGCCGCCCTCTGA
Translation:
MSIFEQGLAPAAVNHIALTPLSFIERTASVYPHYPAVIHGNIRRDWAQTYQRCRRLASALAGRGIGQGDTVA
VMLPNIPEMLEAHYGVPMIGAVLNTLNVRLDAEAIAFMLQHGEAKVLITDYEFHEVIHAAIGMLDHPPLVVD
VNDPEYGEGRPVGELDYEALLAEGDPQFAWEWPRDEWQAISLNYTSGTTGNPKGVVYHHRGAYLNALGNQMT
WSMGQHPVYLWTLPMFHCNGWCYPWTVTALAGVHVCLRRVDPQKVLTLIREHQVSHLCGAPIVLNALINMPD
SAKAAIDHPVHAMVAGAAPPAKVIGAVEEMGIRVTHVYGLTEVYGPVTVCAWHGEWDELPLERRAAIKSRQG
VRYPTLEGVMVADPKTLEPVPRDGQSIGEIFMRGNTVMKGYLKNPSATEEAFAGGWFHTGDLAVCHPDGYIE
IRDRLKDIIISGGENISTIELEGVLYRHPAVLEAAVVARPDEKWGETPCAFITLKSDHQGLAESEIVAFCRE
HLAAFKIPRTVVFSELPKTSTGKIQKYVLREWAAAL*
AnnotationID:591GeneID:582
AnnotatorUID: diggins
Modification Date/Time: 2005-04-01 05:05:57
Gene Name:
Confidence Code:3
GeneProduct:putative AMP-binding enzyme
Cell Localization:()
Synonyms:PA4198
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:putative Acyl-CoA synthetases (AMP-forming)
Functional Category Confidence Code:5
COGs:COG0318,pfam00501,COG0365
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69154.1|AE016549_3 (AE016787) AMP-binding domain protein [Pseudomonas putida KT2440]
          Length = 540

 Score =  948 bits (2450), Expect = 0.0
 Identities = 449/540 (83%), Positives = 491/540 (90%)

>dbj|BAB53431.1| (AP003011) probable AMP-binding protein [Mesorhizobium loti]
          Length = 546

 Score =  632 bits (1630), Expect = e-180
 Identities = 306/540 (56%), Positives = 378/540 (70%), Gaps = 7/540 (1%)







 
Structural Features:
COG0318, CaiC, Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II 
[Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 534 residues,  98.7% aligned
Score =  415 bits (1068), Expect = 6e-117
Identities = 208/545 (38%), Positives = 269/545 (49%), Gaps = 31/545 (5%)

pfam00501, AMP-binding, AMP-binding enzyme.
CD-Length = 412 residues,  99.8% aligned
Score =  280 bits (716), Expect = 4e-76
Identities = 135/430 (31%), Positives = 191/430 (44%), Gaps = 26/430 (6%)

COG0365, Acs, Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases [Lipid metabolism]
CD-Length = 528 residues,  90.7% aligned
Score =  237 bits (605), Expect = 3e-63
Identities = 133/505 (26%), Positives = 197/505 (39%), Gaps = 36/505 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1167




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18309




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36915




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:582_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:582
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
995
                           PA4198_tr
                           translation of PA4198
                           540
                           99.81
                           99.99
                           0
                           2887.0
     

     
     
39324
                           gb|AAG07585.1|AE004836_4
                           (AE004836) probable AMP-binding enzyme [Pseudomonas aeruginosa PAO1]
                           540
                           99.81
                           99.99
                           0
                           2887.0
     

     
     
39325
                           gb|AAN69154.1|AE016549_3
                           (AE016787) AMP-binding domain protein [Pseudomonas putida KT2440]
                           540
                           83.14
                           90.92
                           0
                           2450.0
     

     
     
39326
                           emb|CAE28222.1|
                           (BX572602) possible AMP-binding enzyme [Rhodopseudomonas palustris CGA009]
                           550
                           58.36
                           72.90
                           11
                           1759.0
     

     
     
39327
                           dbj|BAC49778.1|
                           (AP005951) blr4513 [Bradyrhizobium japonicum USDA 110]
                           558
                           57.88
                           73.29
                           21
                           1750.0
     

     






      

          
GC ORFID: 43584How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4198Glimmer Score: 

          
Start: 4123278Stop: 4124900

          
Length: 1623


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4198 translation of PA4198Homolog Bit Score 1116.0

          
Other Homologs:  t_PA4198 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGATCTTCGAACAGGGTCTCGCGCCCGCCGCCGTCAACCACATCGCCCTGACGCCGCTGAGCTTCATC 
GAGCGCACCGCCAGCGTCTATCCGCACTACCCCGCGGTGATCCACGGCAACATCCGCCGCGACTGGGCGCAG 
ACCTACCAACGCTGCCGACGCCTGGCCTCGGCCCTGGCCGGGCGCGGCATCGGCCAGGGCGACACGGTGGCG 
GTGATGCTGCCGAACATCCCCGAGATGCTCGAGGCGCACTATGGCGTGCCGATGATCGGCGCGGTGCTGAAC 
ACCCTCAACGTACGCCTCGACGCCGAAGCCATCGCCTTCATGCTGCAACACGGCGAAGCCAAGGTGCTGATC 
ACCGACTACGAATTCCACGAGGTGATCCATGCCGCCATCGGCATGCTCGACCACCCGCCGCTGGTGGTCGAC 
GTCAACGACCCCGAGTATGGCGAAGGCCGCCCGGTTGGCGAACTGGACTACGAGGCGCTGCTCGCCGAGGGC 
GACCCGCAGTTCGCCTGGGAATGGCCCCGGGACGAGTGGCAGGCGATCTCGCTGAACTACACCTCGGGCACC 
ACCGGCAACCCCAAGGGCGTGGTCTACCATCACCGCGGCGCCTACCTGAACGCGCTGGGCAACCAGATGACC 
TGGTCCATGGGCCAGCACCCGGTGTACCTCTGGACCCTGCCGATGTTCCACTGCAACGGCTGGTGCTATCCG 
TGGACGGTGACCGCGCTGGCCGGCGTCCACGTCTGCCTGCGGCGGGTCGATCCGCAGAAGGTCCTCACCCTG 
ATCCGCGAGCACCAGGTCAGCCACCTGTGCGGCGCACCCATCGTGCTCAACGCCCTGATCAACATGCCCGAC 
TCGGCCAAGGCCGCCATCGACCACCCGGTGCATGCCATGGTCGCCGGCGCCGCGCCACCGGCCAAGGTGATC 
GGCGCGGTGGAAGAGATGGGCATCCGCGTCACCCATGTCTACGGCCTCACCGAGGTCTACGGCCCGGTCACG 
GTCTGCGCCTGGCACGGCGAGTGGGACGAACTGCCGCTGGAGCGGCGCGCCGCGATCAAGTCGCGCCAGGGC 
GTGCGCTACCCGACCCTCGAAGGGGTGATGGTGGCCGACCCGAAGACCCTCGAGCCGGTGCCGCGCGACGGC 
CAGTCCATCGGCGAGATCTTCATGCGCGGCAATACCGTGATGAAGGGCTACCTGAAGAATCCCAGCGCCACC 
GAGGAAGCCTTCGCCGGCGGCTGGTTCCATACCGGCGACCTGGCGGTCTGCCATCCGGACGGCTACATCGAG 
ATCCGCGACCGGCTCAAGGACATCATCATCTCCGGCGGCGAAAACATCTCCACCATCGAACTGGAGGGCGTG 
CTCTACCGCCACCCGGCGGTGCTGGAGGCGGCGGTGGTGGCGCGGCCCGACGAAAAGTGGGGGGAAACGCCC 
TGTGCGTTCATTACCCTGAAGAGCGATCATCAGGGGCTCGCCGAGAGCGAGATCGTCGCGTTCTGTCGCGAA 
CACCTGGCCGCGTTCAAGATTCCGCGTACCGTGGTGTTCTCGGAACTGCCGAAGACCTCCACCGGCAAGATC 
CAGAAATACGTGCTGCGCGAGTGGGCCGCCGCCCTCTGA