ViewGene
Locus Name:
GeneID:
Location:

GeneID:581
TrivialName: PA14_40770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_40770
PAO1 Ortholog Locus:PA1838
Sequence Length:1659
Protein Length:552
Start: 3637871
Stop: 3639529
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05227.1|AE004609_14 (AE004609) sulfite reductase [Pseudomonas aeruginosa PAO1]
Identities = 552/552 (100%), Positives = 552/552 (100%)
Sequence:
ATGTACGTATACGACGAGTACGACCAACGGATCGTCGAGGACCGCGTCAAGCAGTTCCGCGACCAGACCCGC
CGTTACCTGGCGGGTGAATTGACCGGTGAGGAGTTCCGCCCGCTGCGCCTGCAGAACGGTCTCTATATCCAG
CGCTATGCCCCGATGCTGCGCATCGCCGTTCCCTATGGCCTGCTCTCCTCGCGCCAACTGCGCAAGCTGGCG
CAGATCGCCCGCGACTACGACAAGGGCTACGCCCACATCAGCACCCGGCAGAACGTGCAGTTCAACTGGCCG
GAGCTGGAAGACGTGCCGGAAATCCTCGCCGAGCTGGCCACCGTGCAGATGCACGCGATTCAGACCAGCGGC
AACTGCATCCGCAACACCACCACCGACCAGTTCGCCGGCGTCGCCCGCGACGAGCTGGTGGACCCGCGCCCC
TGGTGCGAGATCATCCGCCAATGGTCGACCTTCCACCCCGAGTTCGCCCACCTGCCGCGCAAGTTCAAGATC
GCGGTAAACGGCGCGGTGAGCGACCGTGCCGCCATCGAAGTGCACGACATCGGCCTGGAGGCGGTGAAGAAC
GAGGCCGGCGAGCTCGGCTTCCGCGTCTCGGTCGGCGGCGGCCTGGGCCGAACCCCCATCGTCGGCAGCTTC
ATCAATGAATTCCTGCCCTGGCAGCACCTGCTCGGCTACCTGGATGCCATCCTGCGCGTCTACAACCGCTAT
GGCCGGCGCGACAACAAGTACAAGGCGCGGATCAAGATCCTGGTCAAGGCGCTGACCCCGGAAGTCTTCGCC
GAGCGCGTCGAGGCCGAATGGGCCAACCTCAAGGACGGACCGAGCACCCTCACCGAGGCGGAAGTCCAGCGC
GTCGCCGCGCATTTCGTCGATCCGGCCTACAAGGCCCTCGACGACCTCGATGAATCCCTCGCCCGGCTCGAC
GCGGAGCACCCCGGCTTCGCCCGCTGGCGCGCACGCAACACCTTCGCCCACAAGAAGCCCGGCTACGTCGCC
GTGACGCTCTCGCTGAAGCCCACCGGCACCGCGCCGGGAGACGTCACCGACAAACAGCTGGAGGCCATCGCC
GATCTCGCCGACCGCTACAGCTTCGGCGAGGTGCGCAACAGCCATAACCAGAACATCATTCTCGCCGACGTC
GAGCAGGGCGAACTCTTCGCCCTCTGGGGCGAACTGCGCGAACTGGGCTTCGCCACCCCCAACGTCGGCCTG
CTGACCGACATCATCTGCTGCCCGGGCGGTGACTTCTGCTCCCTGGCCAACGCCAAGTCGATCCCGATCGCC
GAGGCCATCCAGCGCCGCTTCGACAACCTCGACTACCTGTTCGACATCGGCGACATCGACCTGAACATCTCC
GGCTGCATGAACGCCTGCGGCCATCATCATGTCGGCCACATCGGCATCCTCGGGGTCGACAAGAAAGGCGCG
GAGTTCTACCAGGTCTCCCTCGGCGGCAGCTCGGCGCGCGACGCCAGCCTCGGGCAGATCCTCGGCCCGTCC
TTCGCCCAGGAGCAGATGGCCGACGTGATCGACAAGATCATCCAGGTCTACGTGGAACGCCGGACCGAGGAA
GAACCCTTCATCGACACCTTCCGCCGTATCGGTATCGACCCGTTCAAGGAGCGCGTATATGCAGCGAATCAT
TAA
Translation:
MYVYDEYDQRIVEDRVKQFRDQTRRYLAGELTGEEFRPLRLQNGLYIQRYAPMLRIAVPYGLLSSRQLRKLA
QIARDYDKGYAHISTRQNVQFNWPELEDVPEILAELATVQMHAIQTSGNCIRNTTTDQFAGVARDELVDPRP
WCEIIRQWSTFHPEFAHLPRKFKIAVNGAVSDRAAIEVHDIGLEAVKNEAGELGFRVSVGGGLGRTPIVGSF
INEFLPWQHLLGYLDAILRVYNRYGRRDNKYKARIKILVKALTPEVFAERVEAEWANLKDGPSTLTEAEVQR
VAAHFVDPAYKALDDLDESLARLDAEHPGFARWRARNTFAHKKPGYVAVTLSLKPTGTAPGDVTDKQLEAIA
DLADRYSFGEVRNSHNQNIILADVEQGELFALWGELRELGFATPNVGLLTDIICCPGGDFCSLANAKSIPIA
EAIQRRFDNLDYLFDIGDIDLNISGCMNACGHHHVGHIGILGVDKKGAEFYQVSLGGSSARDASLGQILGPS
FAQEQMADVIDKIIQVYVERRTEEEPFIDTFRRIGIDPFKERVYAANH*
AnnotationID:590GeneID:581
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 14:02:23
Gene Name:cysI
Confidence Code:1
GeneProduct:sulfite reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:sulphite reductase hemoprotein, beta subunit
Functional Category Confidence Code:5
COGs:COG0155
Secondary Functional Category(ies):inorganic ion transport and metabolism
EC Number:1.8.1.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56231.1| (AE016865) sulphite reductase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 552

 Score =  969 bits (2504), Expect = 0.0
 Identities = 461/552 (83%), Positives = 512/552 (92%)

>gb|AAN67984.1|AE016430_8 (AE016782) sulphite reductase hemoprotein, beta subunit
           [Pseudomonas putida KT2440]
          Length = 550

 Score =  962 bits (2487), Expect = 0.0
 Identities = 465/552 (84%), Positives = 513/552 (92%), Gaps = 2/552 (0%)

>emb|CAE35979.1| (BX640424) putative sulfite reductase [Bordetella parapertussis]
          Length = 580

 Score =  757 bits (1954), Expect = 0.0
 Identities = 375/566 (66%), Positives = 451/566 (79%), Gaps = 19/566 (3%)






 
Structural Features:
COG0155, CysI, Sulfite reductase, beta subunit (hemoprotein) 
[Inorganic ion transport and metabolism]
CD-Length = 510 residues, 100.0% aligned
Score =  514 bits (1326), Expect = 9e-147
Identities = 210/555 (37%), Positives = 284/555 (51%), Gaps = 53/555 (9%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1165




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18307




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37091




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:581_cdd.html


    		




 
URL:

















    ReferenceID:37793




  
Author/Investigator(s):
        Brandt R, Gunther E, Herrmann H.





  
Title:
        Mapping of cysteine genes on the chromosome of Pseudomonas aeruginosa PAO.




  
PubMed:
        6441099


MedLine:
        




    
Source:
Mol Gen Genet. 1984;197(2):292-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6441099

















    ReferenceID:37794




  
Author/Investigator(s):
        Hummerjohann J, Kuttel E, Quadroni M, Ragaller J, Leisinger T, Kertesz MA.





  
Title:
        Regulation of the sulfate starvation response in Pseudomonas aeruginosa: role of cysteine biosynthetic intermediates.




  
PubMed:
        9611812


MedLine:
        




    
Source:
Microbiology. 1998 May;144 ( Pt 5):1375-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=98274746&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:581
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
993
                           PA1838_tr
                           translation of PA1838
                           552
                           99.99
                           99.99
                           0
                           2890.0
     

     
     
39300
                           gb|AAG05227.1|AE004609_14
                           (AE004609) sulfite reductase [Pseudomonas aeruginosa PAO1]
                           552
                           99.99
                           99.99
                           0
                           2890.0
     

     
     
39301
                           gb|AAC46385.1|
                           (AF026066) sulfite reductase [Pseudomonas aeruginosa]
                           552
                           97.10
                           97.28
                           0
                           2788.0
     

     
     
39303
                           gb|AAN67984.1|AE016430_8
                           (AE016782) sulphite reductase hemoprotein, beta subunit [Pseudomonas putida KT2440]
                           552
                           84.23
                           92.93
                           2
                           2489.0
     

     
     
39302
                           gb|AAO56231.1|
                           (AE016865) sulphite reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           552
                           83.51
                           92.75
                           0
                           2504.0
     

     






      

          
GC ORFID: 43583How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1838Glimmer Score: 

          
Start: 1316039Stop: 1314381

          
Length: 1659


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA1838 translation of PA1838Homolog Bit Score 1117.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene111 (ORF: BLASTX 971.0), t_PA4130 (ORF: BLASTX 703.0), t_PA1838 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTACGTATACGACGAGTACGACCAACGGATCGTCGAGGACCGCGTCAAGCAGTTCCGCGACCAGACCCGC 
CGTTACCTGGCGGGTGAATTGACCGGTGAGGAGTTCCGCCCGCTGCGCCTGCAGAACGGTCTCTATATCCAG 
CGCTATGCCCCGATGCTGCGCATCGCCGTTCCCTATGGCCTGCTCTCCTCGCGCCAACTGCGCAAGCTGGCG 
CAGATCGCCCGCGACTACGACAAGGGCTACGCCCACATCAGCACCCGGCAGAACGTGCAGTTCAACTGGCCG 
GAGCTGGAAGACGTGCCGGAAATCCTCGCCGAGCTGGCCACCGTGCAGATGCACGCGATTCAGACCAGCGGC 
AACTGCATCCGCAACACCACCACCGACCAGTTCGCCGGCGTCGCCCGCGACGAGCTGGTGGACCCGCGCCCC 
TGGTGCGAGATCATCCGCCAATGGTCGACCTTCCACCCCGAGTTCGCCCACCTGCCGCGCAAGTTCAAGATC 
GCGGTAAACGGCGCGGTGAGCGACCGTGCCGCCATCGAAGTGCACGACATCGGCCTGGAGGCGGTGAAGAAC 
GAGGCCGGCGAGCTCGGCTTCCGCGTCTCGGTCGGCGGCGGCCTGGGCCGAACCCCCATCGTCGGCAGCTTC 
ATCAATGAATTCCTGCCCTGGCAGCACCTGCTCGGCTACCTGGATGCCATCCTGCGCGTCTACAACCGCTAT 
GGCCGGCGCGACAACAAGTACAAGGCGCGGATCAAGATCCTGGTCAAGGCGCTGACCCCGGAAGTCTTCGCC 
GAGCGCGTCGAGGCCGAATGGGCCAACCTCAAGGACGGACCGAGCACCCTCACCGAGGCGGAAGTCCAGCGC 
GTCGCCGCGCATTTCGTCGATCCGGCCTACAAGGCCCTCGACGACCTCGATGAATCCCTCGCCCGGCTCGAC 
GCGGAGCACCCCGGCTTCGCCCGCTGGCGCGCACGCAACACCTTCGCCCACAAGAAGCCCGGCTACGTCGCC 
GTGACGCTCTCGCTGAAGCCCACCGGCACCGCGCCGGGAGACGTCACCGACAAACAGCTGGAGGCCATCGCC 
GATCTCGCCGACCGCTACAGCTTCGGCGAGGTGCGCAACAGCCATAACCAGAACATCATTCTCGCCGACGTC 
GAGCAGGGCGAACTCTTCGCCCTCTGGGGCGAACTGCGCGAACTGGGCTTCGCCACCCCCAACGTCGGCCTG 
CTGACCGACATCATCTGCTGCCCGGGCGGTGACTTCTGCTCCCTGGCCAACGCCAAGTCGATCCCGATCGCC 
GAGGCCATCCAGCGCCGCTTCGACAACCTCGACTACCTGTTCGACATCGGCGACATCGACCTGAACATCTCC 
GGCTGCATGAACGCCTGCGGCCATCATCATGTCGGCCACATCGGCATCCTCGGGGTCGACAAGAAAGGCGCG 
GAGTTCTACCAGGTCTCCCTCGGCGGCAGCTCGGCGCGCGACGCCAGCCTCGGGCAGATCCTCGGCCCGTCC 
TTCGCCCAGGAGCAGATGGCCGACGTGATCGACAAGATCATCCAGGTCTACGTGGAACGCCGGACCGAGGAA 
GAACCCTTCATCGACACCTTCCGCCGTATCGGTATCGACCCGTTCAAGGAGCGCGTATATGCAGCGAATCAT 
TAA