ViewGene
Locus Name:
GeneID:
Location:

GeneID:572
TrivialName: PA14_31500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31500
PAO1 Ortholog Locus:PA2555
Sequence Length:1668
Protein Length:555
Start: 2738366
Stop: 2740033
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05943.1|AE004683_7 (AE004683) probable AMP-binding enzyme [Pseudomonas aeruginosa PAO1]
Identities = 554/555 (99%), Positives = 554/555 (99%)
Sequence:
ATGCGCGACTACGCCACCACTGCCGCCGGGTTCGACTTGCAGCGGACCGCCGCCGCCACGCTTTCCGGCACG
CTCCAGGCGCTCAACGCCTGCGTCGAATGCTGCGACCGGCATGCCCTGCCGGGTCGGATCGCGTTGTTCTGG
GAGGGCAAGGATGGCAGTCGCGCCAGCTATACCTTCAGCGAGCTGAAAGCGCTCTCCGGACGTTTCGCCAAT
TTCCTCCAGGCCCAGGGCGTACGCCCGGGCGATTGCGTCGCCGGCCTGTTGCCGCGCACGCCCGAGCTGCTG
GTCACCATCCTCGGCGCCTGGCGCCTGGGCGCGGTGTACCAGCCGCTGTTCACCGCCTTCGGCCCGAAGGCC
ATCGAACACCGCGTGGCGACCGCCGGCAGCAAGGTGCTGGTCACCGATGCGGCCAACCGCGACAAGCTCGAC
GAACTCGCCGATCCGCCTCTGGCCGTCACCGTCGGCGGACCGAAGGGGCAGGGCATCCGTCGCGGCGATTTC
AGCTTCTGGGCCGAGCTGGAGCGCTATCCGGCCGAATTCGAGCCGGTTCCGCGCAGCGGCGAGGATCCGTTC
CTGATGATGTTCACCTCCGGCACCACCGGCCTGGCCAAACCGGTGCCGGTGCCGCTCAAGGCGATCCTCGCC
TTCGTCGGCTACCTGCGCGAGGCGGTGGACCTGCGCCCCGAGGACGCCTTCTGGAACCTCGCCGATCCGGGC
TGGGCCTACGGCCTCTACTACGCTGTCGCCGGTCCCCTCGCCATGGGCCATCCGACCACCTTCTACGAAGGG
CCGTTCAGCGTGGAAAGCACCTGCCGGATCATCCGTGACTACGGCATCACCAACCTCGCCGGATCGCCCACC
GCCTATCGCCTGCTGATGGCCGCCGGCGCGGCCGCGGAAACTGCGCTGAAGGGGCGCCTGCGCGCGGTCAGC
AGCGCCGGCGAGCCGTTGACGCCGGAGGTGATCCGCTGGTTCGCCGACAGGCTCGGCACCACCATCCACGAC
CATTACGGGCAGACCGAGCTGGGCATGGTGCTGTGCAACCACCATGCCCTGGCGCATCCGGTGCGGGTCGGC
GCCGCCGGATTCGCCTGTCCCGGGCACCGGGTGGTAGTGCTGGACGACGATCTCAACGAACTGCCGCCCGGC
CAGCCGGGCATCCTCGCCCTCGACCGGCGGCGTTCGCCGCTGATGTGGTTCCCCGGCTACCAGGGCCTGGAA
ACCGCAGCCTTCGTCGGCGACTACTACCTGAGCGGCGATACCGTCGAACTCAACGAAGACGgcAGCATCAGC
TTCGTCGGCCGCGCCGACGACGTCATTACCACCTCTGGCTACCGGGTCGGCCCGTTCGATGTGGAGAGCGCG
CTGATCGAACACCCGGCAGTGATGGAGGCGGCGGTGATCGGCAAGCCCGACCCGGAGCGCACCGAACTGGTC
AAGGCCTTCGTCGTGCTCTGCGCCGGCCACCGGCCGACGCCGGAACTGGCGGACGAACTGCAGCGATACGTG
CGCCAGCGCCTGTCGGCGCACGCCTACCCGCGGGAGATCGAGTTCCTCGAGGAACTGCCGAAGACCCCCAGC
GGCAAGATCCAGCGCTTTCTCCTGCGCAACCAGGAGATCGCCCGGCAACGCGAGGCGGCCCCACGGGCGGCC
GCCGCGAACTGA
Translation:
MRDYATTAAGFDLQRTAAATLSGTLQALNACVECCDRHALPGRIALFWEGKDGSRASYTFSELKALSGRFAN
FLQAQGVRPGDCVAGLLPRTPELLVTILGAWRLGAVYQPLFTAFGPKAIEHRVATAGSKVLVTDAANRDKLD
ELADPPLAVTVGGPKGQGIRRGDFSFWAELERYPAEFEPVPRSGEDPFLMMFTSGTTGLAKPVPVPLKAILA
FVGYLREAVDLRPEDAFWNLADPGWAYGLYYAVAGPLAMGHPTTFYEGPFSVESTCRIIRDYGITNLAGSPT
AYRLLMAAGAAAETALKGRLRAVSSAGEPLTPEVIRWFADRLGTTIHDHYGQTELGMVLCNHHALAHPVRVG
AAGFACPGHRVVVLDDDLNELPPGQPGILALDRRRSPLMWFPGYQGLETAAFVGDYYLSGDTVELNEDGSIS
FVGRADDVITTSGYRVGPFDVESALIEHPAVMEAAVIGKPDPERTELVKAFVVLCAGHRPTPELADELQRYV
RQRLSAHAYPREIEFLEELPKTPSGKIQRFLLRNQEIARQREAAPRAAAAN*
AnnotationID:581GeneID:572
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 22:10:22
Gene Name:
Confidence Code:3
GeneProduct:putative acyl-coenzyme A synthetases/AMP-(fatty) acid ligases
Cell Localization:()
Synonyms:PA2555
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0365,COG0318,pfam00501
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67826.1|AE016414_1 (AE016782) acyl-CoA ligase [Pseudomonas putida KT2440]
          Length = 561

 Score =  769 bits (1985), Expect = 0.0
 Identities = 379/545 (69%), Positives = 439/545 (80%), Gaps = 6/545 (1%)

>dbj|BAB49240.1| (AP002998) acetyl-CoA synthetase [Mesorhizobium loti]
          Length = 559

 Score =  645 bits (1664), Expect = 0.0
 Identities = 322/542 (59%), Positives = 392/542 (72%), Gaps = 1/542 (0%)

>gb|AAN33644.1|AE014543_8 (AE014543) acetyl-CoA synthetase, putative [Brucella suis 1330]
          Length = 556

 Score =  644 bits (1662), Expect = 0.0
 Identities = 319/543 (58%), Positives = 394/543 (72%), Gaps = 1/543 (0%)






 
Structural Features:
COG0365, Acs, Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases [Lipid metabolism]
CD-Length = 528 residues,  97.9% aligned
Score =  485 bits (1251), Expect = 4e-138
Identities = 200/526 (38%), Positives = 271/526 (51%), Gaps = 18/526 (3%)

COG0318, CaiC, Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II 
[Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 534 residues,  95.3% aligned
Score =  298 bits (765), Expect = 7e-82
Identities = 178/517 (34%), Positives = 253/517 (48%), Gaps = 17/517 (3%)

pfam00501, AMP-binding, AMP-binding enzyme. 
CD-Length = 412 residues, 100.0% aligned
Score =  285 bits (730), Expect = 1e-77
Identities = 140/418 (33%), Positives = 193/418 (46%), Gaps = 14/418 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1147




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18289




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33132




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:572_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:572
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
979
                           PA2555_tr
                           translation of PA2555
                           555
                           99.81
                           99.81
                           0
                           2903.0
     

     
     
38885
                           gb|AAG05943.1|AE004683_7
                           (AE004683) probable AMP-binding enzyme [Pseudomonas aeruginosa PAO1]
                           555
                           99.81
                           99.81
                           0
                           2903.0
     

     
     
38887
                           gb|AAK18169.1|AF290950_1
                           (AF290950) FadDx [Pseudomonas putida]
                           570
                           67.36
                           77.71
                           24
                           1991.0
     

     
     
38886
                           gb|AAN67826.1|AE016414_1
                           (AE016782) acyl-CoA ligase [Pseudomonas putida KT2440]
                           570
                           66.84
                           77.71
                           24
                           1992.0
     

     
     
38888
                           emb|CAE27743.1|
                           (BX572600) acyl-CoA synthetase [Rhodopseudomonas palustris CGA009]
                           571
                           61.82
                           72.15
                           24
                           1784.0
     

     






      

          
GC ORFID: 43574How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2555Glimmer Score: 

          
Start: 2215560Stop: 2213893

          
Length: 1668


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2555 translation of PA2555Homolog Bit Score 1122.0

          
Other Homologs:  t_PA2555 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCGACTACGCCACCACTGCCGCCGGGTTCGACTTGCAGCGGACCGCCGCCGCCACGCTTTCCGGCACG 
CTCCAGGCGCTCAACGCCTGCGTCGAATGCTGCGACCGGCATGCCCTGCCGGGTCGGATCGCGTTGTTCTGG 
GAGGGCAAGGATGGCAGTCGCGCCAGCTATACCTTCAGCGAGCTGAAAGCGCTCTCCGGACGTTTCGCCAAT 
TTCCTCCAGGCCCAGGGCGTACGCCCGGGCGATTGCGTCGCCGGCCTGTTGCCGCGCACGCCCGAGCTGCTG 
GTCACCATCCTCGGCGCCTGGCGCCTGGGCGCGGTGTACCAGCCGCTGTTCACCGCCTTCGGCCCGAAGGCC 
ATCGAACACCGCGTGGCGACCGCCGGCAGCAAGGTGCTGGTCACCGATGCGGCCAACCGCGACAAGCTCGAC 
GAACTCGCCGATCCGCCTCTGGCCGTCACCGTCGGCGGACCGAAGGGGCAGGGCATCCGTCGCGGCGATTTC 
AGCTTCTGGGCCGAGCTGGAGCGCTATCCGGCCGAATTCGAGCCGGTTCCGCGCAGCGGCGAGGATCCGTTC 
CTGATGATGTTCACCTCCGGCACCACCGGCCTGGCCAAACCGGTGCCGGTGCCGCTCAAGGCGATCCTCGCC 
TTCGTCGGCTACCTGCGCGAGGCGGTGGACCTGCGCCCCGAGGACGCCTTCTGGAACCTCGCCGATCCGGGC 
TGGGCCTACGGCCTCTACTACGCTGTCGCCGGTCCCCTCGCCATGGGCCATCCGACCACCTTCTACGAAGGG 
CCGTTCAGCGTGGAAAGCACCTGCCGGATCATCCGTGACTACGGCATCACCAACCTCGCCGGATCGCCCACC 
GCCTATCGCCTGCTGATGGCCGCCGGCGCGGCCGCGGAAACTGCGCTGAAGGGGCGCCTGCGCGCGGTCAGC 
AGCGCCGGCGAGCCGTTGACGCCGGAGGTGATCCGCTGGTTCGCCGACAGGCTCGGCACCACCATCCACGAC 
CATTACGGGCAGACCGAGCTGGGCATGGTGCTGTGCAACCACCATGCCCTGGCGCATCCGGTGCGGGTCGGC 
GCCGCCGGATTCGCCTGTCCCGGGCACCGGGTGGTAGTGCTGGACGACGATCTCAACGAACTGCCGCCCGGC 
CAGCCGGGCATCCTCGCCCTCGACCGGCGGCGTTCGCCGCTGATGTGGTTCCCCGGCTACCAGGGCCTGGAA 
ACCGCAGCCTTCGTCGGCGACTACTACCTGAGCGGCGATACCGTCGAACTCAACGAAGACGgcAGCATCAGC 
TTCGTCGGCCGCGCCGACGACGTCATTACCACCTCTGGCTACCGGGTCGGCCCGTTCGATGTGGAGAGCGCG 
CTGATCGAACACCCGGCAGTGATGGAGGCGGCGGTGATCGGCAAGCCCGACCCGGAGCGCACCGAACTGGTC 
AAGGCCTTCGTCGTGCTCTGCGCCGGCCACCGGCCGACGCCGGAACTGGCGGACGAACTGCAGCGATACGTG 
CGCCAGCGCCTGTCGGCGCACGCCTACCCGCGGGAGATCGAGTTCCTCGAGGAACTGCCGAAGACCCCCAGC 
GGCAAGATCCAGCGCTTTCTCCTGCGCAACCAGGAGATCGCCCGGCAACGCGAGGCGGCCCCACGGGCGGCC 
GCCGCGAACTGA