ViewGene
Locus Name:
GeneID:
Location:

GeneID:57
TrivialName: PA14_32390
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_32390
PAO1 Ortholog Locus:PA2494
Sequence Length:3189
Protein Length:1062
Start: 2817342
Stop: 2814154
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05882.1|AE004676_14 (AE004676) RND multidrug efflux transporter MexF [Pseudomonas aeruginosa PAO1]
Identities = 1062/1062 (100%), Positives = 1062/1062 (100%)
Sequence:
ATGAATTTCTCCCAATTCTTCATCCAGCGGCCGATCTTCGCCGCGGTGCTGTCGCTGCTGATCCTGATCGGC
GGCGCCATCTCCCTGTTCCAGCTACCGATCAGCGAATACCCGGAAGTGGTGCCGCCGACCGTCGTGGTCCGC
GCCAACTTCCCCGGCGCCAACCCGAAAGTCATCGGCGAGACCGTCGCCTCTCCCCTTGAACAGGCGATCACC
GGGGTGGAGAACATGCTCTACATGTCCTCCCAGTCGACCTCCGACGGCAAGCTGACCCTGACCATCACCTTC
GCCCTCGGCACCGACCTGGACAACGCCCAGGTGCAGGTGCAGAACCGCGTCACCCGGACCGAGCCGAAGCTC
CCGGAAGAAGTGACCCGGCTCGGCATCACCGTCGACAAGGCCTCGCCCGACCTGACCATGGTCGTGCACCTG
ACCTCGCCGGATAACCGCTACGACATGCTCTACCTGTCGAACTACGCGGTGCTCAACGTGAAGGACGAACTG
GCCCGCCTCGACGGCGTCGGCGACGTCCAGTTGTTCGGCCTCGGCGACTATTCGCTGCGCGTCTGGCTGGAC
CCGAACAAGGTCGCCTCGCGCAACCTCACCGCCACCGACGTGGTCAACGCCATCCGCGAGCAGAACCGCCAG
GTCGCCGCCGGCACCCTGGGCGCGCCGCCGGCGCCGAGCGATACCAGCTTCCAGTTGTCGATCAACACCCAG
GGGCGCCTGGTCACCGAGGAAGAGTTCGAGAACATCATCATCCGCGCCGGCGCCAACGGCGAGATCACCCGT
CTGCGCGACATCGCCCGGGTCGAGCTGGGCTCCAACCAGTACGCCCTGCGTTCGCTGCTGAACAACAAGCCG
GCGGTGGCGATCCCGATCTTCCAGCGTCCCGGCTCGAACGCCATCGAAATCTCCAACCTGGTGCGGGAGAAG
ATGGCCGAGCTGAAGCACAGCTTCCCGCAGGGCATGGACTACTCCATCGTCTACGACCCGACCATCTTCGTC
CGCGGCTCCATCGAGGCGGTGGTGCACACCCTGTTCGAAGCCCTGGTGCTGGTGGTGCTGGTGGTGATCCTG
TTCCTGCAGACCTGGCGCGCCTCGATCATCCCGCTGGCCGCGGTGCCGGTGTCGCTGATCGGCACCTTCGCG
GTGATGCACATGCTCGGCTTCTCGCTCAACGCGCTGTCGCTGTTCGGCCTGGTGCTGGCCATCGGCATCGTG
GTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCAACATCGGCCTCGGCCTCAAGCCGGTGGAAGCC
ACCAAGCGTGCCATGCGCGAGGTGACCGGGCCGATCATCGCCACGGCGCTGGTGCTCTGCGCGGTGTTCATC
CCGACCGCGTTCATCTCCGGCCTCACCGGGCAGTTCTACCGCCAGTTCGCCCTGACCATCGCGATCTCCACG
GTGATCTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCGCTGGCGGCGGTCCTGCTCAAGGGCCACCACGAG
CCGAAGGACCGCTTCTCGGTGTTCCTCGACAAGCTCCTCGGCAGTTGGCTGTTCCGTCCGTTCAACCGTTTC
TTCGACCGCGCCAGCCATGGCTACGTCGGCACGGTGAACCGGGTCCTGCGCGGCAGCTCGATCGCCCTGCTG
GTCTACGGCGGGCTGATGGTGCTGACCTACTTCGGCTTCTCCAGCACGCCGACCGGTTTCGTCCCGCAGCAG
GACAAGCAGTACCTGGTGGCCTTCGCCCAGTTGCCCGACGCGGCCAGCCTGGACCGTACCGAGGCGGTGATC
AAGCAGATGTCCGAGATCGCCCTGGCGCAGCCCGGCGTGGCGGACTCGGTGGCCTTCCCCGGCCTGTCGATC
AACGGCTTCACCAACAGCCCGAACAGCGGCATCGTGTTCACCCCGCTGAAGCCGTTCGACGAGCGCAAGGAC
CCGAGCCAGTCGGCCGGGGCCATCGCCGCCGCGCTGAACGCCAAGTACGCCGACATCCAGGACGCCTACATC
GCGATCTTCCCACCGCCGCCGGTACAGGGGCTGGGGACCATCGGCGGCTTCCGCCTGCAGATCGAGGACCGT
GGCAACCAGGGCTACGAGGAGCTGTTCAAGCAGACCCAGAACATCATCACCAAGGCCCGTGCGCTGCCTGAG
CTGGAACCCAGCTCGGTGTTCTCCAGCTACCAGGTCAACGTGCCGCAGATCGACGCCGACATCGACCGCGAG
AAGGCCAAGACCCACGGCGTGGCGATCAGCGACATCTTCGACACCCTGCAGGTCTACCTCGGCTCGCTGTAC
GCGAACGACTTCAACCGCTTCGGCCGTACCTACCAGGTCAACGTCCAGGCCGAGCAGCAGTTCCGCCTCGAA
CCCGAGCAGATCGGCCAGTTGAAGGTGCGCAACAACCTCGGCGAGATGGTCCCGCTGGCGTCCTTCATCAAG
GTCAGCGACACCTCCGGTCCGGACCGTGTGATGCACTACAACGGCTTCATCACCGCCGAACTCAACGGCGCC
CCGGCCGCGGGCTACAGCTCCGGCCAGGCGCAGGCGGCGATCGAGAAGCTGCTGAAGGAGGAACTGCCCAAC
GGCATGACCTACGAGTGGACCGAGCTGACCTACCAGCAGATCCTCGCCGGCAATACCGCGCTGTTCGTCTTC
CCGCTCTGCGTGCTGCTGGCCTTCCTCGTGCTGGCCGCCCAGTACGAGAGCTGGAGCCTGCCGCTGGCGGTG
ATCCTGATCGTGCCGATGACCCTGCTGTCGGCGATCACCGGGGTGATCCTGGCCGGCAGCGACAACAACATC
TTTACCCAGATCGGCCTGATCGTTCTGGTGGGGCTGGCGTGCAAGAACGCGATCCTGATCGTCGAGTTCGCC
AAGGACAAGCAGGAGGAAGGCATGGACCGCGTCGCCGCGGTGCTGGAAGCCTGCCGCCTGCGCCTGCGGCCG
ATCCTGATGACGTCCATCGCCTTCATCATGGGCGTGGTGCCGCTGGTGATCTCCACCGGCGCCGGCGCCGAG
ATGCGCCATGCGATGGGCGTGGCGGTGTTCTCCGGGATGATCGGGGTGACCTTCTTCGGCCTGCTGCTGACG
CCGGTGTTCTACGTCCTCATCCGCCGCTTCGTGGAGAACCGCGAAGCGCGCCGCGCCGCCAACGACAAAGGC
CTGCCAGAGGTGCATGCATGA
Translation:
MNFSQFFIQRPIFAAVLSLLILIGGAISLFQLPISEYPEVVPPTVVVRANFPGANPKVIGETVASPLEQAIT
GVENMLYMSSQSTSDGKLTLTITFALGTDLDNAQVQVQNRVTRTEPKLPEEVTRLGITVDKASPDLTMVVHL
TSPDNRYDMLYLSNYAVLNVKDELARLDGVGDVQLFGLGDYSLRVWLDPNKVASRNLTATDVVNAIREQNRQ
VAAGTLGAPPAPSDTSFQLSINTQGRLVTEEEFENIIIRAGANGEITRLRDIARVELGSNQYALRSLLNNKP
AVAIPIFQRPGSNAIEISNLVREKMAELKHSFPQGMDYSIVYDPTIFVRGSIEAVVHTLFEALVLVVLVVIL
FLQTWRASIIPLAAVPVSLIGTFAVMHMLGFSLNALSLFGLVLAIGIVVDDAIVVVENVERNIGLGLKPVEA
TKRAMREVTGPIIATALVLCAVFIPTAFISGLTGQFYRQFALTIAISTVISAFNSLTLSPALAAVLLKGHHE
PKDRFSVFLDKLLGSWLFRPFNRFFDRASHGYVGTVNRVLRGSSIALLVYGGLMVLTYFGFSSTPTGFVPQQ
DKQYLVAFAQLPDAASLDRTEAVIKQMSEIALAQPGVADSVAFPGLSINGFTNSPNSGIVFTPLKPFDERKD
PSQSAGAIAAALNAKYADIQDAYIAIFPPPPVQGLGTIGGFRLQIEDRGNQGYEELFKQTQNIITKARALPE
LEPSSVFSSYQVNVPQIDADIDREKAKTHGVAISDIFDTLQVYLGSLYANDFNRFGRTYQVNVQAEQQFRLE
PEQIGQLKVRNNLGEMVPLASFIKVSDTSGPDRVMHYNGFITAELNGAPAAGYSSGQAQAAIEKLLKEELPN
GMTYEWTELTYQQILAGNTALFVFPLCVLLAFLVLAAQYESWSLPLAVILIVPMTLLSAITGVILAGSDNNI
FTQIGLIVLVGLACKNAILIVEFAKDKQEEGMDRVAAVLEACRLRLRPILMTSIAFIMGVVPLVISTGAGAE
MRHAMGVAVFSGMIGVTFFGLLLTPVFYVLIRRFVENREARRAANDKGLPEVHA*
AnnotationID:67GeneID:57
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 15:03:08
Gene Name:mexF
Confidence Code:1
GeneProduct:RND multidrug efflux transporter MexF
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00873,COG0841
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69028.1|AE016535_2 (AE016786) multidrug efflux RND transporter MexF [Pseudomonas putida
            KT2440]
          Length = 1059

 Score = 1834 bits (4750), Expect = 0.0
 Identities = 943/1062 (88%), Positives = 1002/1062 (94%), Gaps = 3/1062 (0%)

>gb|AAN56485.1|AE015785_8 (AE015785) RND multidrug efflux transporter MexF [Shewanella
            oneidensis MR-1]
          Length = 1047

 Score = 1469 bits (3804), Expect = 0.0
 Identities = 750/1039 (72%), Positives = 875/1039 (84%), Gaps = 4/1039 (0%)

>emb|CAE36018.1| (BX640424) putative multidrug-efflux system protein [Bordetella
            parapertussis]
          Length = 1053

 Score = 1307 bits (3383), Expect = 0.0
 Identities = 669/1040 (64%), Positives = 825/1040 (79%), Gaps = 11/1040 (1%)








 
Structural Features:
pfam00873, ACR_tran, AcrB/AcrD/AcrF family.
CD-Length = 1017 residues, 100.0% aligned
Score = 1141 bits (2952), Expect = 0.0
Identities = 483/1041 (46%), Positives = 667/1041 (64%), Gaps = 27/1041 (2%)

COG0841, AcrB, Cation/multidrug efflux pump [Defense mechanisms]
CD-Length = 1009 residues, 100.0% aligned
Score = 1045 bits (2704), Expect = 0.0
Identities = 490/1044 (46%), Positives = 681/1044 (65%), Gaps = 38/1044 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:117




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17259




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33037




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:57_cdd.html


    		




 
URL:

















    ReferenceID:34499




  
Author/Investigator(s):
        Aeschlimann JR.





  
Title:
        The role of multidrug efflux pumps in the antibiotic resistance of Pseudomonas aeruginosa and other gram-negative bacteria. Insights from the Society of Infectious Diseases Pharmacists.




  
PubMed:
        12885104


MedLine:
        




    
Source:
Pharmacotherapy. 2003 Jul;23(7):916-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12885104

















    ReferenceID:34500




  
Author/Investigator(s):
        Aires JR, Pechere JC, Van Delden C, Kohler T.





  
Title:
        Amino acid residues essential for function of the MexF efflux pump protein of Pseudomonas aeruginosa.




  
PubMed:
        12069970


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 2002 Jul;46(7):2169-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12069970









           
Homologs By Global Alignment 

           

       



     
Gene ID:57
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
106
                           PA2494_tr
                           translation of PA2494
                           1062
                           99.99
                           99.99
                           0
                           5313.0
     

     
     
12271
                           gb|AAG05882.1|AE004676_14
                           (AE004676) RND multidrug efflux transporter MexF [Pseudomonas aeruginosa PAO1]
                           1062
                           99.99
                           99.99
                           0
                           5313.0
     

     
     
12272
                           emb|CAA67864.1|
                           (X99514) hypothetical protein [Pseudomonas aeruginosa]
                           1062
                           99.81
                           99.90
                           0
                           5304.0
     

     
     
12273
                           gb|AAN69028.1|AE016535_2
                           (AE016786) multidrug efflux RND transporter MexF [Pseudomonas putida KT2440]
                           1062
                           88.79
                           94.35
                           3
                           4754.0
     

     
     
12274
                           gb|AAO56587.1|
                           (AE016867) multidrug efflux RND transporter MexF [Pseudomonas syringae pv. tomato str. DC3000]
                           1065
                           85.63
                           93.42
                           5
                           4667.0
     

     






      

          
GC ORFID: 43059How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2494Glimmer Score: 

          
Start: 2136582Stop: 2139770

          
Length: 3189


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2494 translation of PA2494Homolog Bit Score 2051.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7199 (ORF: BLASTX 1810.0), t_PA3522 (ORF: BLASTX 1248.0), t_PA2018 (ORF: BLASTX 802.0), t_PA0426 (ORF: BLASTX 797.0), t_PA4598 (ORF: BLASTX 785.0), psyr_15may02_Scaffold5_revised_gene6178 (ORF: BLASTX 765.0), psyr_15may02_Scaffold7_revised_gene7337 (ORF: BLASTX 107.0), t_PA2494 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAATTTCTCCCAATTCTTCATCCAGCGGCCGATCTTCGCCGCGGTGCTGTCGCTGCTGATCCTGATCGGC 
GGCGCCATCTCCCTGTTCCAGCTACCGATCAGCGAATACCCGGAAGTGGTGCCGCCGACCGTCGTGGTCCGC 
GCCAACTTCCCCGGCGCCAACCCGAAAGTCATCGGCGAGACCGTCGCCTCTCCCCTTGAACAGGCGATCACC 
GGGGTGGAGAACATGCTCTACATGTCCTCCCAGTCGACCTCCGACGGCAAGCTGACCCTGACCATCACCTTC 
GCCCTCGGCACCGACCTGGACAACGCCCAGGTGCAGGTGCAGAACCGCGTCACCCGGACCGAGCCGAAGCTC 
CCGGAAGAAGTGACCCGGCTCGGCATCACCGTCGACAAGGCCTCGCCCGACCTGACCATGGTCGTGCACCTG 
ACCTCGCCGGATAACCGCTACGACATGCTCTACCTGTCGAACTACGCGGTGCTCAACGTGAAGGACGAACTG 
GCCCGCCTCGACGGCGTCGGCGACGTCCAGTTGTTCGGCCTCGGCGACTATTCGCTGCGCGTCTGGCTGGAC 
CCGAACAAGGTCGCCTCGCGCAACCTCACCGCCACCGACGTGGTCAACGCCATCCGCGAGCAGAACCGCCAG 
GTCGCCGCCGGCACCCTGGGCGCGCCGCCGGCGCCGAGCGATACCAGCTTCCAGTTGTCGATCAACACCCAG 
GGGCGCCTGGTCACCGAGGAAGAGTTCGAGAACATCATCATCCGCGCCGGCGCCAACGGCGAGATCACCCGT 
CTGCGCGACATCGCCCGGGTCGAGCTGGGCTCCAACCAGTACGCCCTGCGTTCGCTGCTGAACAACAAGCCG 
GCGGTGGCGATCCCGATCTTCCAGCGTCCCGGCTCGAACGCCATCGAAATCTCCAACCTGGTGCGGGAGAAG 
ATGGCCGAGCTGAAGCACAGCTTCCCGCAGGGCATGGACTACTCCATCGTCTACGACCCGACCATCTTCGTC 
CGCGGCTCCATCGAGGCGGTGGTGCACACCCTGTTCGAAGCCCTGGTGCTGGTGGTGCTGGTGGTGATCCTG 
TTCCTGCAGACCTGGCGCGCCTCGATCATCCCGCTGGCCGCGGTGCCGGTGTCGCTGATCGGCACCTTCGCG 
GTGATGCACATGCTCGGCTTCTCGCTCAACGCGCTGTCGCTGTTCGGCCTGGTGCTGGCCATCGGCATCGTG 
GTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCAACATCGGCCTCGGCCTCAAGCCGGTGGAAGCC 
ACCAAGCGTGCCATGCGCGAGGTGACCGGGCCGATCATCGCCACGGCGCTGGTGCTCTGCGCGGTGTTCATC 
CCGACCGCGTTCATCTCCGGCCTCACCGGGCAGTTCTACCGCCAGTTCGCCCTGACCATCGCGATCTCCACG 
GTGATCTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCGCTGGCGGCGGTCCTGCTCAAGGGCCACCACGAG 
CCGAAGGACCGCTTCTCGGTGTTCCTCGACAAGCTCCTCGGCAGTTGGCTGTTCCGTCCGTTCAACCGTTTC 
TTCGACCGCGCCAGCCATGGCTACGTCGGCACGGTGAACCGGGTCCTGCGCGGCAGCTCGATCGCCCTGCTG 
GTCTACGGCGGGCTGATGGTGCTGACCTACTTCGGCTTCTCCAGCACGCCGACCGGTTTCGTCCCGCAGCAG 
GACAAGCAGTACCTGGTGGCCTTCGCCCAGTTGCCCGACGCGGCCAGCCTGGACCGTACCGAGGCGGTGATC 
AAGCAGATGTCCGAGATCGCCCTGGCGCAGCCCGGCGTGGCGGACTCGGTGGCCTTCCCCGGCCTGTCGATC 
AACGGCTTCACCAACAGCCCGAACAGCGGCATCGTGTTCACCCCGCTGAAGCCGTTCGACGAGCGCAAGGAC 
CCGAGCCAGTCGGCCGGGGCCATCGCCGCCGCGCTGAACGCCAAGTACGCCGACATCCAGGACGCCTACATC 
GCGATCTTCCCACCGCCGCCGGTACAGGGGCTGGGGACCATCGGCGGCTTCCGCCTGCAGATCGAGGACCGT 
GGCAACCAGGGCTACGAGGAGCTGTTCAAGCAGACCCAGAACATCATCACCAAGGCCCGTGCGCTGCCTGAG 
CTGGAACCCAGCTCGGTGTTCTCCAGCTACCAGGTCAACGTGCCGCAGATCGACGCCGACATCGACCGCGAG 
AAGGCCAAGACCCACGGCGTGGCGATCAGCGACATCTTCGACACCCTGCAGGTCTACCTCGGCTCGCTGTAC 
GCGAACGACTTCAACCGCTTCGGCCGTACCTACCAGGTCAACGTCCAGGCCGAGCAGCAGTTCCGCCTCGAA 
CCCGAGCAGATCGGCCAGTTGAAGGTGCGCAACAACCTCGGCGAGATGGTCCCGCTGGCGTCCTTCATCAAG 
GTCAGCGACACCTCCGGTCCGGACCGTGTGATGCACTACAACGGCTTCATCACCGCCGAACTCAACGGCGCC 
CCGGCCGCGGGCTACAGCTCCGGCCAGGCGCAGGCGGCGATCGAGAAGCTGCTGAAGGAGGAACTGCCCAAC 
GGCATGACCTACGAGTGGACCGAGCTGACCTACCAGCAGATCCTCGCCGGCAATACCGCGCTGTTCGTCTTC 
CCGCTCTGCGTGCTGCTGGCCTTCCTCGTGCTGGCCGCCCAGTACGAGAGCTGGAGCCTGCCGCTGGCGGTG 
ATCCTGATCGTGCCGATGACCCTGCTGTCGGCGATCACCGGGGTGATCCTGGCCGGCAGCGACAACAACATC 
TTTACCCAGATCGGCCTGATCGTTCTGGTGGGGCTGGCGTGCAAGAACGCGATCCTGATCGTCGAGTTCGCC 
AAGGACAAGCAGGAGGAAGGCATGGACCGCGTCGCCGCGGTGCTGGAAGCCTGCCGCCTGCGCCTGCGGCCG 
ATCCTGATGACGTCCATCGCCTTCATCATGGGCGTGGTGCCGCTGGTGATCTCCACCGGCGCCGGCGCCGAG 
ATGCGCCATGCGATGGGCGTGGCGGTGTTCTCCGGGATGATCGGGGTGACCTTCTTCGGCCTGCTGCTGACG 
CCGGTGTTCTACGTCCTCATCCGCCGCTTCGTGGAGAACCGCGAAGCGCGCCGCGCCGCCAACGACAAAGGC 
CTGCCAGAGGTGCATGCATGA