ViewGene
Locus Name:
GeneID:
Location:

GeneID:569
TrivialName: PA14_62620
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62620
PAO1 Ortholog Locus:PA4732
Sequence Length:1665
Protein Length:554
Start: 5585606
Stop: 5587270
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08118.1|AE004887_2 (AE004887) glucose-6-phosphate isomerase [Pseudomonas aeruginosa PAO1]
Identities = 554/554 (100%), Positives = 554/554 (100%)
Sequence:
ATGAAGCACCACCTCACTCCGCTGGATGCCACGCAACTCGACAGTTGGCGCGCCCTGGCCGCCCATCGCCAG
GAACTGCAGGACTTCAGGATGCGCCAGGCGTTCATCGACGATCCGGAGCGCTTCAAGCGGTTTTCCTTCAGC
GCCTGTGGGCTGTTCCTCGACTTCTCGAAGAACCTGATTCGCCAGGACACCATCGACCTGCTGGTGAAGCTG
GCCGAGGAGGCACGGCTGAGCGACGCGATCAGGGCGATGTTCGACGGCGAGGCGATCAATGCCTCCGAACGC
CGGCCGGTACTGCATACCGCGCTGCGTCGACCGATCGGCGACAAGGTGCTGGTCGATGGCGTCGACGTGATG
CCGGAAGTCCACCGCGTCCTGCACCAGATGACCGAGCTGGTGGGCTACGTGCACAACGGCCTGTGGCGCGGC
TATACCGAAAAGCCGATCACCGACGTGGTGAACATCGGCATCGGCGGCTCCTTCCTCGGCCCGCAACTGGTA
TCCGAAGCCTTGCTGCCGTTCGCCCAGAAAGGCGTGCGCTGCCACTACCTGGCGAACATCGACGGTAGCGAG
TTCCATGAACTGGCCAGCCGCCTGAACGCCGAGACCACCCTGTTCATCGTTTCCTCCAAGTCCTTCGGGACC
CTGGAAACCCTGAAGAACGCCCAGGCCGCACGCGCCTGGTACCTGGCCCAGGGCGGCACCGAGGAAGAGCTC
TACCGCCATTTCATCGCCGTCTCCAGCAACAAGGAAGCCGCGATCGCCTTCGGCATCCGCGAGGAAAACATC
TTCCCCATGTGGGACTGGGTCGGCGGCCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCATGTCC
ATCGGCATCTCCAACTTCAAGGAACTGCTGTCCGGCGCCTACAACATGGACCAGCATTTCCAGACCGCGCCG
TTCGAACGCAACATCCCGGTGCTGCTCGGCCTGCTCGGCGTCTGGTACGGCGACTTCTGGGGCGCGAACAGC
CACGCGATCCTGCCCTACGACTACTATCTGCGGAATATCACCGACCACCTGCAACAACTGGACATGGAATCC
AACGGCAAGAGCGTGCGCCAGGACGGCACGCCGGTCACCAGCGGCACCGGGCCGGTGATCTGGGGTGGTGTC
GGCTGCAACGGCCAGCACGCCTACCATCAGTTGCTGCACCAGGGCACCCAGTTGATCCCGGCGGACTTCATC
GTCCCGGTGAGCAGCTACAACCCGGTGGCCGACCATCACCAGTGGCTGTACGCCAACTGCCTGTCGCAGAGC
CAGGCGCTGATGCTCGGCAAGAGCCGCGAGGAGGCCGAGGCCGAACTGCGCGCCAAGGGCCTGCCGGAAGCC
GAAGTGCAGCGCCTGGCGCCGCACAAGGTGATCCCGGGCAACCGACCGAGCAACACCCTGGTGGTCGAGCGC
ATCAGCGCCCGCCGCCTCGGCGCGCtGATCGCCATGTACGAGCACAAGGTCTACGTACAGAGCATCCTCTGG
GGCATCAACGCCTTCGACCAATGGGGCGTGGAGCTTGGCAAGGAACTCGGCAAGGGTGTCTATTCGCGCCTG
GTCGGCAGCGAGGAAACCCCGGCCGAGGACGCCTCCACCCAAGGCCTGATCGACTTCTTCCGCGGCCGCCAT
CGCGGCTGA
Translation:
MKHHLTPLDATQLDSWRALAAHRQELQDFRMRQAFIDDPERFKRFSFSACGLFLDFSKNLIRQDTIDLLVKL
AEEARLSDAIRAMFDGEAINASERRPVLHTALRRPIGDKVLVDGVDVMPEVHRVLHQMTELVGYVHNGLWRG
YTEKPITDVVNIGIGGSFLGPQLVSEALLPFAQKGVRCHYLANIDGSEFHELASRLNAETTLFIVSSKSFGT
LETLKNAQAARAWYLAQGGTEEELYRHFIAVSSNKEAAIAFGIREENIFPMWDWVGGRYSLWSAIGLPIAMS
IGISNFKELLSGAYNMDQHFQTAPFERNIPVLLGLLGVWYGDFWGANSHAILPYDYYLRNITDHLQQLDMES
NGKSVRQDGTPVTSGTGPVIWGGVGCNGQHAYHQLLHQGTQLIPADFIVPVSSYNPVADHHQWLYANCLSQS
QALMLGKSREEAEAELRAKGLPEAEVQRLAPHKVIPGNRPSNTLVVERISARRLGALIAMYEHKVYVQSILW
GINAFDQWGVELGKELGKGVYSRLVGSEETPAEDASTQGLIDFFRGRHRG*
AnnotationID:578GeneID:569
AnnotatorUID: diggins
Modification Date/Time: 2005-05-18 21:09:58
Gene Name:pgi
Confidence Code:2
GeneProduct:glucose-6-phosphate isomerase
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00342,COG0166
Secondary Functional Category(ies):central intermediary metabolism
EC Number:5.3.1.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70274.1|AE016667_7 (AE016791) glucose-6-phosphate isomerase [Pseudomonas putida
           KT2440]
          Length = 554

 Score =  937 bits (2423), Expect = 0.0
 Identities = 450/554 (81%), Positives = 501/554 (90%)

>gb|AAN67427.1|AE016370_2 (AE016780) glucose-6-phosphate isomerase [Pseudomonas putida
           KT2440]
          Length = 554

 Score =  930 bits (2403), Expect = 0.0
 Identities = 446/554 (80%), Positives = 499/554 (90%)

>gb|AAN56538.1|AE015791_2 (AE015791) glucose-6-phosphate isomerase [Shewanella oneidensis
           MR-1]
          Length = 545

 Score =  627 bits (1617), Expect = e-179
 Identities = 298/539 (55%), Positives = 394/539 (73%), Gaps = 2/539 (0%)






 
Structural Features:
pfam00342, PGI, Phosphoglucose isomerase.
CD-Length = 483 residues, 100.0% aligned
Score =  564 bits (1455), Expect = 1e-161
Identities = 252/491 (51%), Positives = 331/491 (67%), Gaps = 8/491 (1%)

COG0166, Pgi, Glucose-6-phosphate isomerase 
[Carbohydrate transport and metabolism]
CD-Length = 446 residues,  99.6% aligned
Score =  495 bits (1277), Expect = 4e-141
Identities = 227/493 (46%), Positives = 296/493 (60%), Gaps = 50/493 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1141




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18283




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37646




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:569_cdd.html


    		




 
URL:

















    ReferenceID:37734




  
Author/Investigator(s):
        Calligeros JE, Matsumoto H, Phibbs PV Jr, Gates JE.





  
Title:
        Characterization and genetic mapping of phosphoglucoisomerase mutations in mannitol-negative mutants of Pseudomonas aeruginosa.




  
PubMed:
        8900099


MedLine:
        




    
Source:
Curr Microbiol. 1996 Dec;33(6):347-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8900099

















    ReferenceID:37735




  
Author/Investigator(s):
        Froman BE, Tait RC, Gottlieb LD.





  
Title:
        Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli.




  
PubMed:
        2549364


MedLine:
        




    
Source:
Mol Gen Genet. 1989 May;217(1):126-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2549364









           
Homologs By Global Alignment 

           

       



     
Gene ID:569
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
976
                           PA4732_tr
                           translation of PA4732
                           554
                           99.99
                           99.99
                           0
                           2911.0
     

     
     
38594
                           gb|AAG08118.1|AE004887_2
                           (AE004887) glucose-6-phosphate isomerase [Pseudomonas aeruginosa PAO1]
                           554
                           99.99
                           99.99
                           0
                           2911.0
     

     
     
38595
                           gb|AAN70274.1|AE016667_7
                           (AE016791) glucose-6-phosphate isomerase [Pseudomonas putida KT2440]
                           554
                           81.22
                           90.43
                           0
                           2423.0
     

     
     
38596
                           gb|AAO60163.1|
                           (AY210414) glucose-6-phosphate isomerase; Pgi [Pseudomonas fluorescens]
                           554
                           80.50
                           90.97
                           0
                           2413.0
     

     
     
38597
                           gb|AAN67427.1|AE016370_2
                           (AE016780) glucose-6-phosphate isomerase [Pseudomonas putida KT2440]
                           554
                           80.50
                           90.07
                           0
                           2403.0
     

     






      

          
GC ORFID: 43571How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4732Glimmer Score: 

          
Start: 4849286Stop: 4850950

          
Length: 1665


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4732 translation of PA4732Homolog Bit Score 1125.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2522 (ORF: BLASTX 921.0), t_PA4732 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCACCACCTCACTCCGCTGGATGCCACGCAACTCGACAGTTGGCGCGCCCTGGCCGCCCATCGCCAG 
GAACTGCAGGACTTCAGGATGCGCCAGGCGTTCATCGACGATCCGGAGCGCTTCAAGCGGTTTTCCTTCAGC 
GCCTGTGGGCTGTTCCTCGACTTCTCGAAGAACCTGATTCGCCAGGACACCATCGACCTGCTGGTGAAGCTG 
GCCGAGGAGGCACGGCTGAGCGACGCGATCAGGGCGATGTTCGACGGCGAGGCGATCAATGCCTCCGAACGC 
CGGCCGGTACTGCATACCGCGCTGCGTCGACCGATCGGCGACAAGGTGCTGGTCGATGGCGTCGACGTGATG 
CCGGAAGTCCACCGCGTCCTGCACCAGATGACCGAGCTGGTGGGCTACGTGCACAACGGCCTGTGGCGCGGC 
TATACCGAAAAGCCGATCACCGACGTGGTGAACATCGGCATCGGCGGCTCCTTCCTCGGCCCGCAACTGGTA 
TCCGAAGCCTTGCTGCCGTTCGCCCAGAAAGGCGTGCGCTGCCACTACCTGGCGAACATCGACGGTAGCGAG 
TTCCATGAACTGGCCAGCCGCCTGAACGCCGAGACCACCCTGTTCATCGTTTCCTCCAAGTCCTTCGGGACC 
CTGGAAACCCTGAAGAACGCCCAGGCCGCACGCGCCTGGTACCTGGCCCAGGGCGGCACCGAGGAAGAGCTC 
TACCGCCATTTCATCGCCGTCTCCAGCAACAAGGAAGCCGCGATCGCCTTCGGCATCCGCGAGGAAAACATC 
TTCCCCATGTGGGACTGGGTCGGCGGCCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCATGTCC 
ATCGGCATCTCCAACTTCAAGGAACTGCTGTCCGGCGCCTACAACATGGACCAGCATTTCCAGACCGCGCCG 
TTCGAACGCAACATCCCGGTGCTGCTCGGCCTGCTCGGCGTCTGGTACGGCGACTTCTGGGGCGCGAACAGC 
CACGCGATCCTGCCCTACGACTACTATCTGCGGAATATCACCGACCACCTGCAACAACTGGACATGGAATCC 
AACGGCAAGAGCGTGCGCCAGGACGGCACGCCGGTCACCAGCGGCACCGGGCCGGTGATCTGGGGTGGTGTC 
GGCTGCAACGGCCAGCACGCCTACCATCAGTTGCTGCACCAGGGCACCCAGTTGATCCCGGCGGACTTCATC 
GTCCCGGTGAGCAGCTACAACCCGGTGGCCGACCATCACCAGTGGCTGTACGCCAACTGCCTGTCGCAGAGC 
CAGGCGCTGATGCTCGGCAAGAGCCGCGAGGAGGCCGAGGCCGAACTGCGCGCCAAGGGCCTGCCGGAAGCC 
GAAGTGCAGCGCCTGGCGCCGCACAAGGTGATCCCGGGCAACCGACCGAGCAACACCCTGGTGGTCGAGCGC 
ATCAGCGCCCGCCGCCTCGGCGCGCtGATCGCCATGTACGAGCACAAGGTCTACGTACAGAGCATCCTCTGG 
GGCATCAACGCCTTCGACCAATGGGGCGTGGAGCTTGGCAAGGAACTCGGCAAGGGTGTCTATTCGCGCCTG 
GTCGGCAGCGAGGAAACCCCGGCCGAGGACGCCTCCACCCAAGGCCTGATCGACTTCTTCCGCGGCCGCCAT 
CGCGGCTGA