ViewGene

Locus Name:
GeneID:
Location:

GeneID: 5620

TrivialName: PA14_49520

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_49520

PAO1 Ortholog Locus:

Sequence Length: 2376

Protein Length: 791

Start: 4405534

Stop: 4403159

Strand: -

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: emb|CAA54958.1| (X77996) killer protein of pyocin S3 [Pseudomonas aeruginosa] Identities = 760/766 (99%), Positives = 760/766 (99%)

Sequence:

TTGGATTCCAGTGGGCACATATTCCAGCAAGAATACCTCTGTATATTTTCTCAAGCTCAAATCGAGGTAAAA
ACTATGGCTGATGCACCACCGCCACTGATTGTTAGATACTCAAGTCGCCCCGGGAAACCTGGCGGAGTTAGC
ATAGACGTATCACGAGACAGGCAGGACATACTAAGCAACTATCCGTTCGGTAACCCTTTCCGTGGTTTTACT
CTGGCCATGGAGGCTGACTTCAAAAAGAGGAAAGAGAGTCTGGAAGCCGACGTTCAGGCAGAGTTGGATACG
GAAGCTGCCAAGTATCCGGCACTACCTGCCAGCTTCACACCTGAGCAGTGGCTGGAAAGATCAACCGTAGTT
GTCACCGGGCTGCTAAATCGAAAGCGCCCTGAACTAGATAACGAATTGGATGTGGTTCAGAACTCGGCTAGT
CTGGATAAGCTGCAAGCAAGCTACAACACAATCATCCTGAATGATCAGATCAATAGTCTTCAAGAGCGCTTG
AACAAGCTCAATGCTGAAACCGACCGCCGCCGGGCGGAGGCAGAAGCCCAGCGTCAGGCAGAAGCGGACGCC
AAACGCAAAGCGGAAGAAGCGGCACGCCTGGAAGCCCAGCAGGTCACTCAACAGCTCGGTGCCGACTTCAAC
GCGATCACCACTCCGACCGCCACCAAGGTCCAGCAACGTGTGAAAGCAGTGGATGCTAGTCTGTCTCAGGTT
TCCACCCAGGTCAGTGGTGCAGTCGCCTCGGCAACCCAGGCGGTGCAGGTCAAGACAGCGCAAGCCCAGCAA
CAAGCCAACAGCCAGATTTCGTCCAGCCAGAACCTGATCAGCGCGGCATTTCGGAATCAGATTGCCTTGGCA
GCACAGGCCAGTGGAGAAGCCCAGGTCACGGCTTTCAACACCAAAGTCAAGCAGATCGTCAACGAAGCCACG
GCGTTTTCGACAGCACGAAAACAAGCGCTGGCTCAGTTTGCTGCCAATGCGGCCGCCGAGGTCGAAACCGAG
GTCAACAGGCTAACCGCCCAGGTCAAGGCCAGCCCCACCAAGGCGGCTTCGCAAGCTGCACAAGCAACCCTT
CGACAATTCACTGAAAGCACCTATGCCAAGGTGAACACCTTTGCCCTGCAGACCGAAGCAGCGCTGCAGGCC
AAAGCCAAGGAGGTTTCGGCAGCGGTAGCCGAGGCACAGCGTGCGGCGACCAACGATTTGCAAAAAACAGCC
GATGTAGTACCAGGTCAGATCATTCAGGCTGCGAACACCTTGGCGATGCCGGCCACCTCTACGCCGCTGGTT
TCGGTGGCCGGGTTTGGTAGTGCGGCAGTGGAGACGGCTCGCCTGGCGGCTTCCCTAACGAATGCTGTCAAT
CGCTTGATTCAGATTGCTGGTAGTGGACCTGGCGCATACGTGGCCACCTTCGCGGTGCTATCGCTCTACTCC
GACCAGGCTGGTAAAGACAGTGACAAGGTTCCTGCCGGCGTTCGCAATGCATTGGCACTGGAGGCGTCCGCT
CTGGGGCTTCCTGGCACGGCTGATTTGCAAAGCGTCGCCAAGGCAGGTGGCACGGTTGATATGCCGGTACGA
CTCACGAGTGCTGCACAAGAGAGCCCGAGTGGTAAATCGCAGATTGCCGCGATGCTGACCAACGGGGCAACT
GTCCCCAAAGGCGTGCCTGTTCGCGCCGCGACCCTCAATACTGCGACAGGCCGGTATGAGGTGACGGTTCCC
GCAAAGTCCACCGTGCCGAATACACCCCCGCTGATCTTGACCTGGACCCCTGCCACCCCTCCAGGAAGCCAG
AACCCCTCAAGCACCACTCCGGTCGTACCGCAGCCGGCTCCGGTGTATGAGGGAGCAACGATTACCCCAGTG
CAGGCCGAACCGGAGTCCTATCCGGGAGTGCCGTTAGATCTGGACGATCTGATCGTGATCTTCCCGGCGGGC
TCGGACGTTCCTCCGCTCTATCTGGTCTTTTCCAAGCCGCCGGTTAAGCCGCTGGAAGTGGACATTTATGGT
AATTTTGCAGGAAGACCTCGTGATGGAAACCATCTTGACCATATGCCGGCTCAAGGTGCGTTGGCGACGAGT
TTGAGAGCTATTTATCCAGACATCCCCTATGGAGAAATTCGCAAATTAATGAAGAAAGGGGGGAGTGTTGCC
ATACCAGCTAGAGTGCATCAGAGGTTCAGTGAAACCTACGGAGGACGTAACACCAAAGAAAAGCAACAGAAG
GATGCCTCCGACTTGCGTGCAGCAGTCGATAGCAATTTCGATGCCGTCAAGAAAGGATTGCTAGAGGAGGGG
TTCTCCGAAGATGATATTGAGAGGGCGCGCGCAGAACTACACATGGTGAACAAAGAACAGGGGTGGTACTGA

Translation:

MDSSGHIFQQEYLCIFSQAQIEVKTMADAPPPLIVRYSSRPGKPGGVSIDVSRDRQDILSNYPFGNPFRGFT
LAMEADFKKRKESLEADVQAELDTEAAKYPALPASFTPEQWLERSTVVVTGLLNRKRPELDNELDVVQNSAS
LDKLQASYNTIILNDQINSLQERLNKLNAETDRRRAEAEAQRQAEADAKRKAEEAARLEAQQVTQQLGADFN
AITTPTATKVQQRVKAVDASLSQVSTQVSGAVASATQAVQVKTAQAQQQANSQISSSQNLISAAFRNQIALA
AQASGEAQVTAFNTKVKQIVNEATAFSTARKQALAQFAANAAAEVETEVNRLTAQVKASPTKAASQAAQATL
RQFTESTYAKVNTFALQTEAALQAKAKEVSAAVAEAQRAATNDLQKTADVVPGQIIQAANTLAMPATSTPLV
SVAGFGSAAVETARLAASLTNAVNRLIQIAGSGPGAYVATFAVLSLYSDQAGKDSDKVPAGVRNALALEASA
LGLPGTADLQSVAKAGGTVDMPVRLTSAAQESPSGKSQIAAMLTNGATVPKGVPVRAATLNTATGRYEVTVP
AKSTVPNTPPLILTWTPATPPGSQNPSSTTPVVPQPAPVYEGATITPVQAEPESYPGVPLDLDDLIVIFPAG
SDVPPLYLVFSKPPVKPLEVDIYGNFAGRPRDGNHLDHMPAQGALATSLRAIYPDIPYGEIRKLMKKGGSVA
IPARVHQRFSETYGGRNTKEKQQKDASDLRAAVDSNFDAVKKGLLEEGFSEDDIERARAELHMVNKEQGWY*

AnnotationID:5622 GeneID:5620

AnnotatorUID: danlee

Modification Date/Time: 2005-09-28 15:03:36

Gene Name: pyoS3A

Confidence Code: 1

GeneProduct: pyocin killing protein

Cell Localization: (9) Secreted

Synonyms:

Cell Localization Confidence Code: 1

MolecularFunction:

Functional Category: (23) Secreted Factors (toxins, enzymes, alginate)

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: pfam06958

Secondary Functional Category(ies): Adaptation, protection

EC Number:

Status: ACTIVE

Pathway:

Homology:

760/766 (99%) identical to pir||A56394: pyocin S3  - Pseudomonas aeruginosa (strain P12)
482/671 (71%) identical (523/671 similar) to pir||F83161: pyocin protein PA3866 - Pseudomonas 
aeruginosa  (strain PAO1)

Structural Features:

pfam06958, S-type Pyocin. Pyocins are polypeptide toxins produced by, and active against, bacteria. 
S-type pyocins cause cell death by DNA breakdown due to endonuclease activity.
     CD-Length = 166 residues,  95.8% aligned
     Score =  178 bits (452), Expect = 2e-45

Genomic Context:

Comment:

PAO1 has PA1150 (pys2 = pyocin S2) at this chromosomal location; however, this PA14 gene
more closely resembles PA3866 (pyocin protein).

ReferenceID:15480

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:28375

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:38195

Author/Investigator(s): Duport,C., Baysse,C. and Michel-Briand,Y.

Title: Molecular characterization of pyocin S3, a novel S-type pyocin from Pseudomonas aeruginosa

PubMed: 7721800

MedLine:

Source:

J. Biol. Chem. 270 (15), 8920-8927 (1995)

Reference Type: Journal

Data:

URL:

Homologs By Global Alignment

Gene ID:5620

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

197332 emb|CAA54958.1| (X77996) killer protein of pyocin S3 [Pseudomonas aeruginosa] 791 96.08 96.08 25 3764.0

7902 PA3866_tr translation of PA3866 844 59.71 66.46 133 2209.0

197333 gb|AAG07253.1|AE004804_1 (AE004804) pyocin protein [Pseudomonas aeruginosa PAO1] 844 59.71 66.46 133 2209.0

197334 emb|CAE13179.1| (BX571861) unnamed protein product [Photorhabdus luminescens subsp. laumondii TTO1] 826 24.21 34.14 304 481.0

GC ORFID: `48622`	How Found: `Glimmer`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name:	Glimmer Score:
Start: `548374`	Stop: `550749`
Length: `2376`
Start Codon: `TTG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA3866 translation of PA3866`	Homolog Bit Score `845.0`
Other Homologs: `t_PA3866 translation of PA3866 (BLASTP 845)`
GC ORF Sequence
TTGGATTCCAGTGGGCACATATTCCAGCAAGAATACCTCTGTATATTTTCTCAAGCTCAAATCGAGGTAAAA ACTATGGCTGATGCACCACCGCCACTGATTGTTAGATACTCAAGTCGCCCCGGGAAACCTGGCGGAGTTAGC ATAGACGTATCACGAGACAGGCAGGACATACTAAGCAACTATCCGTTCGGTAACCCTTTCCGTGGTTTTACT CTGGCCATGGAGGCTGACTTCAAAAAGAGGAAAGAGAGTCTGGAAGCCGACGTTCAGGCAGAGTTGGATACG GAAGCTGCCAAGTATCCGGCACTACCTGCCAGCTTCACACCTGAGCAGTGGCTGGAAAGATCAACCGTAGTT GTCACCGGGCTGCTAAATCGAAAGCGCCCTGAACTAGATAACGAATTGGATGTGGTTCAGAACTCGGCTAGT CTGGATAAGCTGCAAGCAAGCTACAACACAATCATCCTGAATGATCAGATCAATAGTCTTCAAGAGCGCTTG AACAAGCTCAATGCTGAAACCGACCGCCGCCGGGCGGAGGCAGAAGCCCAGCGTCAGGCAGAAGCGGACGCC AAACGCAAAGCGGAAGAAGCGGCACGCCTGGAAGCCCAGCAGGTCACTCAACAGCTCGGTGCCGACTTCAAC GCGATCACCACTCCGACCGCCACCAAGGTCCAGCAACGTGTGAAAGCAGTGGATGCTAGTCTGTCTCAGGTT TCCACCCAGGTCAGTGGTGCAGTCGCCTCGGCAACCCAGGCGGTGCAGGTCAAGACAGCGCAAGCCCAGCAA CAAGCCAACAGCCAGATTTCGTCCAGCCAGAACCTGATCAGCGCGGCATTTCGGAATCAGATTGCCTTGGCA GCACAGGCCAGTGGAGAAGCCCAGGTCACGGCTTTCAACACCAAAGTCAAGCAGATCGTCAACGAAGCCACG GCGTTTTCGACAGCACGAAAACAAGCGCTGGCTCAGTTTGCTGCCAATGCGGCCGCCGAGGTCGAAACCGAG GTCAACAGGCTAACCGCCCAGGTCAAGGCCAGCCCCACCAAGGCGGCTTCGCAAGCTGCACAAGCAACCCTT CGACAATTCACTGAAAGCACCTATGCCAAGGTGAACACCTTTGCCCTGCAGACCGAAGCAGCGCTGCAGGCC AAAGCCAAGGAGGTTTCGGCAGCGGTAGCCGAGGCACAGCGTGCGGCGACCAACGATTTGCAAAAAACAGCC GATGTAGTACCAGGTCAGATCATTCAGGCTGCGAACACCTTGGCGATGCCGGCCACCTCTACGCCGCTGGTT TCGGTGGCCGGGTTTGGTAGTGCGGCAGTGGAGACGGCTCGCCTGGCGGCTTCCCTAACGAATGCTGTCAAT CGCTTGATTCAGATTGCTGGTAGTGGACCTGGCGCATACGTGGCCACCTTCGCGGTGCTATCGCTCTACTCC GACCAGGCTGGTAAAGACAGTGACAAGGTTCCTGCCGGCGTTCGCAATGCATTGGCACTGGAGGCGTCCGCT CTGGGGCTTCCTGGCACGGCTGATTTGCAAAGCGTCGCCAAGGCAGGTGGCACGGTTGATATGCCGGTACGA CTCACGAGTGCTGCACAAGAGAGCCCGAGTGGTAAATCGCAGATTGCCGCGATGCTGACCAACGGGGCAACT GTCCCCAAAGGCGTGCCTGTTCGCGCCGCGACCCTCAATACTGCGACAGGCCGGTATGAGGTGACGGTTCCC GCAAAGTCCACCGTGCCGAATACACCCCCGCTGATCTTGACCTGGACCCCTGCCACCCCTCCAGGAAGCCAG AACCCCTCAAGCACCACTCCGGTCGTACCGCAGCCGGCTCCGGTGTATGAGGGAGCAACGATTACCCCAGTG CAGGCCGAACCGGAGTCCTATCCGGGAGTGCCGTTAGATCTGGACGATCTGATCGTGATCTTCCCGGCGGGC TCGGACGTTCCTCCGCTCTATCTGGTCTTTTCCAAGCCGCCGGTTAAGCCGCTGGAAGTGGACATTTATGGT AATTTTGCAGGAAGACCTCGTGATGGAAACCATCTTGACCATATGCCGGCTCAAGGTGCGTTGGCGACGAGT TTGAGAGCTATTTATCCAGACATCCCCTATGGAGAAATTCGCAAATTAATGAAGAAAGGGGGGAGTGTTGCC ATACCAGCTAGAGTGCATCAGAGGTTCAGTGAAACCTACGGAGGACGTAACACCAAAGAAAAGCAACAGAAG GATGCCTCCGACTTGCGTGCAGCAGTCGATAGCAATTTCGATGCCGTCAAGAAAGGATTGCTAGAGGAGGGG TTCTCCGAAGATGATATTGAGAGGGCGCGCGCAGAACTACACATGGTGAACAAAGAACAGGGGTGGTACTGA