ViewGene
Locus Name:
GeneID:
Location:

GeneID:551
TrivialName: PA14_66840
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66840
PAO1 Ortholog Locus:PA5058
Sequence Length:1683
Protein Length:560
Start: 5969743
Stop: 5971425
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08443.1|AE004919_4 (AE004919) poly(3-hydroxyalkanoic acid) synthase 2 [Pseudomonas aeruginosa PAO1]
Identities = 558/560 (99%), Positives = 560/560 (100%)
Sequence:
ATGCGAGAAAAACAGGAATCGGGTAGCGTGCCGGTGCCCGCCGAGTTCATGAGTGCGCAGAGCGCCATCGTC
GGCCTGCGCGGCAAGGACCTGCTGACGACGGTCCGCAGCCTGGCCGTCCACGGCCTGCGCCAGCCGCTGCAC
AGTGCGCGGCACCTGGTCGCCTTCGGAGGCCAGTTGGGCAAGGTGCTGCTGGGCGACACCCTGCACCAGCCG
AACCCTCAGGACGCCCGTTTCCAGGATCCATCCTGGCGCCTCAATCCCTTCTACCGGCGCACCCTGCAGGCC
TACCTGGCGTGGCAGAAGCAACTGCTCGCCTGGATCGACGAAAGCAACCTGGACTGCGACGATCGCGCCCGC
GCCCGCTTCCTCGTCGCCTTGCTCTCCGACGCCGTGGCACCCAGCAACAGCCTGATCAATCCACTGGCGGTA
AAGGAACTGTTCAATACCGGCGGGATCAGCCTGCTCAATGGCGTCCGCCACCTGCTCGAAGACCTGGTGCAC
AACGGCGGCATGCCCAGCCAGGTGAACAAGACCGCCTTCGAGATCGGCCGCAACCTCGCCACCACGCAAGGC
GCGGTAGTGTTCCGCAACGAGGTGCTGGAACTGATCCAGTACAAGCCGCTGGGCGAGCGCCAGTACGCCAAG
CCCCTGCTGATCGTGCCGCCGCAGATCAACAAGTACTACATCTTCGACCTGTCGCCGGAAAAGAGCTTCGTC
CAGTACGCCCTGAAGAACAACCTGCAGGTCTTCGTCATCAGTTGGCGCAACCCCGACGCCCAGCACCGCGAA
TGGGGCCTGAGCACCTATGTCGAGGCCCTAGACCAAGCCATCGAGGTCAGCCGCGAGATCACCGGCAGCCGC
AGCGTGAACCTGGCCGGCGCCTGCGCCGGCGGGCTCACCGTGGCCGCCTTGCTCGGCcACCTCCAGGTGCGC
CGGCAACTGCGCAAGGTCAGTAGCGTCACCTACCTGGTCAGCCTGCTCGACAGCCAGATGGAAAGCCCGGCG
ATGCTCTTCGCCGACGAGCAGACCCTGGAGAGCAGCAAGCGCCGCTCCTACCAGCATGGCGTGCTGGACGGG
CGCGACATGGCCAAGGTGTTCGCCTGGATGCGCCCCAACGACCTGATCTGGAACTACTGGGTCAACAACTAC
CTGCTCGGCAGGCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACAACACGCGGCTGCCCGCGGCG
TTCCACGGCGAACTGCTCGACCTGTTCAAGCACAACCCGCTGACCCGCCCGGGCGCGCTGGAAGTCAGCGGG
ACCGCGGTGGACCTGGGCAAGGTGGCGATCGATAGCTTCCACGTCGCCGGCATCACCGACCACATCACGCCC
TGGGACGCGGTCTATCGCTCGGCCCTCCTGCTGGGCGGCCAGCGCCGCTTCATCCTGTCCAACAGCGGGCAC
ATCCAGAGCATCCTCAACCCTCCCGGAAACCCCAAGGCCTGCTACTTCGAGAACGACAAGCTGAGCAGCGAT
CCACGCGCCTGGTACTACGACGCCAAGCGCGAAGAGGGCAGTTGGTGGCCGGTCTGGCTGGGCTGGCTGCAG
GAGCGCTCGGGCGAGCTGGGCGACCCTGACTTCAACCTTGGCAGCGCCGCGCATCCGCCCCTCGAAGCAGCC
CCGGGCACCTACGTGCATATACGCTGA
Translation:
MREKQESGSVPVPAEFMSAQSAIVGLRGKDLLTTVRSLAVHGLRQPLHSARHLVAFGGQLGKVLLGDTLHQP
NPQDARFQDPSWRLNPFYRRTLQAYLAWQKQLLAWIDESNLDCDDRARARFLVALLSDAVAPSNSLINPLAV
KELFNTGGISLLNGVRHLLEDLVHNGGMPSQVNKTAFEIGRNLATTQGAVVFRNEVLELIQYKPLGERQYAK
PLLIVPPQINKYYIFDLSPEKSFVQYALKNNLQVFVISWRNPDAQHREWGLSTYVEALDQAIEVSREITGSR
SVNLAGACAGGLTVAALLGHLQVRRQLRKVSSVTYLVSLLDSQMESPAMLFADEQTLESSKRRSYQHGVLDG
RDMAKVFAWMRPNDLIWNYWVNNYLLGRQPPAFDILYWNNDNTRLPAAFHGELLDLFKHNPLTRPGALEVSG
TAVDLGKVAIDSFHVAGITDHITPWDAVYRSALLLGGQRRFILSNSGHIQSILNPPGNPKACYFENDKLSSD
PRAWYYDAKREEGSWWPVWLGWLQERSGELGDPDFNLGSAAHPPLEAAPGTYVHIR*
AnnotationID:560GeneID:551
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 11:11:20
Gene Name:phaC2
Confidence Code:1
GeneProduct:poly(3-hydroxyalkanoic acid) synthase 2
Cell Localization:()
Synonyms:phaC
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:PHA-synthase2
Functional Category Confidence Code:5
COGs:COG3243,pfam07167
Secondary Functional Category(ies):lipid metabolism
EC Number:2.3.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO59384.1| (AY278219) PHA synthase 2 [Pseudomonas stutzeri]
          Length = 560

 Score =  920 bits (2379), Expect = 0.0
 Identities = 434/560 (77%), Positives = 498/560 (88%)

>gb|AAD26367.2|AF129396_3 (AF129396) PHA synthase 2 [Pseudomonas resinovorans]
          Length = 560

 Score =  910 bits (2352), Expect = 0.0
 Identities = 429/560 (76%), Positives = 496/560 (88%)

>gb|AAK19608.1|AF336849_2 (AF336849) PHA synthase 2 [Pseudomonas nitroreducens]
          Length = 560

 Score =  894 bits (2310), Expect = 0.0
 Identities = 419/560 (74%), Positives = 488/560 (87%)








 
Structural Features:
COG3243, PhaC, Poly(3-hydroxyalkanoate) synthetase [Lipid metabolism]
CD-Length = 445 residues, 100.0% aligned
Score =  559 bits (1443), Expect = 2e-160
Identities = 231/456 (50%), Positives = 294/456 (64%), Gaps = 14/456 (3%)

pfam07167, PhaC_N, Poly-beta-hydroxybutyrate polymerase (PhaC) N-terminus.
CD-Length = 172 residues, 100.0% aligned
Score =  260 bits (667), Expect = 2e-70
Identities = 94/172 (54%), Positives = 120/172 (69%), Gaps = 2/172 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1105




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18247




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35032




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:551_cdd.html


    		




 
URL:

















    ReferenceID:35828




  
Author/Investigator(s):
        Qi Q, Rehm BH, Steinbuchel A.





  
Title:
        Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2.




  
PubMed:
        9418250


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1997 Dec 1;157(1):155-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9418250

















    ReferenceID:35829




  
Author/Investigator(s):
        Timm A, Steinbuchel A.





  
Title:
        Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1.




  
PubMed:
        1396693


MedLine:
        




    
Source:
Eur J Biochem. 1992 Oct 1;209(1):15-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=93011120&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:551
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
952
                           PA5058_tr
                           translation of PA5058
                           560
                           99.64
                           99.99
                           0
                           2943.0
     

     
     
38157
                           gb|AAG08443.1|AE004919_4
                           (AE004919) poly(3-hydroxyalkanoic acid) synthase 2 [Pseudomonas aeruginosa PAO1]
                           560
                           99.64
                           99.99
                           0
                           2943.0
     

     
     
38158
                           emb|CAA47153.1|
                           (X66592) PHA-synthase2 [Pseudomonas aeruginosa]
                           560
                           99.64
                           99.99
                           0
                           2943.0
     

     
     
38159
                           gb|AAQ72541.1|
                           (AY370935) MCL-PHA synthase 2 [Pseudomonas sp. HJ-2]
                           560
                           79.82
                           90.35
                           0
                           2450.0
     

     
     
38160
                           gb|AAO59384.1|
                           (AY278219) PHA synthase 2 [Pseudomonas stutzeri]
                           560
                           77.50
                           88.92
                           0
                           2379.0
     

     






      

          
GC ORFID: 43553How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5058Glimmer Score: 

          
Start: 5233426Stop: 5235108

          
Length: 1683


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5058 translation of PA5058Homolog Bit Score 1138.0

          
Other Homologs:  t_PA5056 (ORF: BLASTX 691.0), t_PA5058 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGAGAAAAACAGGAATCGGGTAGCGTGCCGGTGCCCGCCGAGTTCATGAGTGCGCAGAGCGCCATCGTC 
GGCCTGCGCGGCAAGGACCTGCTGACGACGGTCCGCAGCCTGGCCGTCCACGGCCTGCGCCAGCCGCTGCAC 
AGTGCGCGGCACCTGGTCGCCTTCGGAGGCCAGTTGGGCAAGGTGCTGCTGGGCGACACCCTGCACCAGCCG 
AACCCTCAGGACGCCCGTTTCCAGGATCCATCCTGGCGCCTCAATCCCTTCTACCGGCGCACCCTGCAGGCC 
TACCTGGCGTGGCAGAAGCAACTGCTCGCCTGGATCGACGAAAGCAACCTGGACTGCGACGATCGCGCCCGC 
GCCCGCTTCCTCGTCGCCTTGCTCTCCGACGCCGTGGCACCCAGCAACAGCCTGATCAATCCACTGGCGGTA 
AAGGAACTGTTCAATACCGGCGGGATCAGCCTGCTCAATGGCGTCCGCCACCTGCTCGAAGACCTGGTGCAC 
AACGGCGGCATGCCCAGCCAGGTGAACAAGACCGCCTTCGAGATCGGCCGCAACCTCGCCACCACGCAAGGC 
GCGGTAGTGTTCCGCAACGAGGTGCTGGAACTGATCCAGTACAAGCCGCTGGGCGAGCGCCAGTACGCCAAG 
CCCCTGCTGATCGTGCCGCCGCAGATCAACAAGTACTACATCTTCGACCTGTCGCCGGAAAAGAGCTTCGTC 
CAGTACGCCCTGAAGAACAACCTGCAGGTCTTCGTCATCAGTTGGCGCAACCCCGACGCCCAGCACCGCGAA 
TGGGGCCTGAGCACCTATGTCGAGGCCCTAGACCAAGCCATCGAGGTCAGCCGCGAGATCACCGGCAGCCGC 
AGCGTGAACCTGGCCGGCGCCTGCGCCGGCGGGCTCACCGTGGCCGCCTTGCTCGGCcACCTCCAGGTGCGC 
CGGCAACTGCGCAAGGTCAGTAGCGTCACCTACCTGGTCAGCCTGCTCGACAGCCAGATGGAAAGCCCGGCG 
ATGCTCTTCGCCGACGAGCAGACCCTGGAGAGCAGCAAGCGCCGCTCCTACCAGCATGGCGTGCTGGACGGG 
CGCGACATGGCCAAGGTGTTCGCCTGGATGCGCCCCAACGACCTGATCTGGAACTACTGGGTCAACAACTAC 
CTGCTCGGCAGGCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACAACACGCGGCTGCCCGCGGCG 
TTCCACGGCGAACTGCTCGACCTGTTCAAGCACAACCCGCTGACCCGCCCGGGCGCGCTGGAAGTCAGCGGG 
ACCGCGGTGGACCTGGGCAAGGTGGCGATCGATAGCTTCCACGTCGCCGGCATCACCGACCACATCACGCCC 
TGGGACGCGGTCTATCGCTCGGCCCTCCTGCTGGGCGGCCAGCGCCGCTTCATCCTGTCCAACAGCGGGCAC 
ATCCAGAGCATCCTCAACCCTCCCGGAAACCCCAAGGCCTGCTACTTCGAGAACGACAAGCTGAGCAGCGAT 
CCACGCGCCTGGTACTACGACGCCAAGCGCGAAGAGGGCAGTTGGTGGCCGGTCTGGCTGGGCTGGCTGCAG 
GAGCGCTCGGGCGAGCTGGGCGACCCTGACTTCAACCTTGGCAGCGCCGCGCATCCGCCCCTCGAAGCAGCC 
CCGGGCACCTACGTGCATATACGCTGA