ViewGene
Locus Name:
GeneID:
Location:

GeneID:547
TrivialName: PA14_20030
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20030
PAO1 Ortholog Locus:PA3406
Sequence Length:1791
Protein Length:596
Start: 1725533
Stop: 1727323
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06794.1|AE004761_10 (AE004761) transport protein HasD [Pseudomonas aeruginosa PAO1]
Identities = 593/600 (98%), Positives = 594/600 (99%), Gaps = 4/600 (0%)
Sequence:
ATGAAGCTCCGGCCGGCCTCGCCGCGCAATGAAATCCTCGCCACCCTCGGCCGTTTCCGCCCGGCGCTGCGC
AGCGTCGCGCTGTTCACCGCGGTGATCAACCTGCTGATGCTGGCGCCGTCGCTGTACATGCTGCAGGTCTAC
GACCGGGTGCTCGGCTCCGGCAACCACATGACCCTGCTGATGCTGACCCTCATGGTGCTCGGCCTCTACCTG
CTGCTCGGCGCCCTGGAGTGGGTGCGCAGCCTGGTGGTGATCCGCCTCGGCGGCCAGCTCGACATGCAACTC
AACCAGCGCATCTACGACGCCTCGTTCCGCGCCAGCCTGGAGCGCGGCGAGCAGGCCGCCGGGCAGGCGCTG
AACGACCTGACCAGCCTGCGCCAGTTCCTCACCGGCAACGCCCTGTTCGCCTTCTTCGACGCGCCCTGGTTC
CCGCTCTACCTGCTGGTGATCTTCCTCTTCAGTCCCTGGCTCGGCCTGCTCGCCCTGGCCGGGGCACTGCTA
CTGGTGCTGCTCGCCTGGGTCAACGAAAGCCGCTCGCGCGAACCGCTGGCCGAGGCCGGGCAACTGTCGATC
CTCGCCACCCAGCAGGCCTCGGCCAACCTGCGCCAGGCGGAAACCCTGGCGGCAATGGGCATGTTGCCGGCC
ATGCGCGCGCGCTGGTTCGCCCAGCACCAGGCCTTCCTTGCCCGGCAGAACCTCGGCAGCGAGCGCAGCGCG
GCGATCGGCGCGACCTCCAAGGGCGTGCGCCTGGCCTTGCAGTCGCTGGTGCTCGGCCTCGGCGCCTGGCTG
GCGGTGGAAGGCCTGATCACCCCGGGGATGATGATCGCCGGCTCGATCCTGATGGGCCGCGTGCTCAGCCCC
ATCGACCAGTTGATCGCGGTCTGGCGCCAGTGGAGCGGCGCGCGGCAGGCCTACCAGCGACTGGCGCGGCTG
CTCGAGGAGAATCCGCCTGCCGCCCTGGGCATGCCGCTGCCGGTGCCGCGCGGCGCGTTGCGCGTCGAGCGG
CTCTGCGCGGCCGCGCCGGGACGCGAGCAGGCATTGTTGCAGGACCTCGGTTTCGCCCTGGAGCCTGGCGAA
GCGCTCGGCGTGATCGGGCCGTCCGGTTCCGGCAAGTCGACCCTGGCGCGCTTGCTGGTGGGCGCCTGGCAG
CCGCTGTCCGGCGCGGTGCGCCTGGACGGCGCCGACCTGCGGCAGTGGAGTGCGGCGGCACTGGGGCCGCAC
ATCGGCTACCTGGCCCAGGACGTGCAACTGTTCGCCGGCAGCATCGCCGAGAACATCGCGCGCTTCGCCGAG
GTGGATGCCGAGAAGGTGGTGGCGGCGGCGCGCCTGGCCGGCGTGCACGACCTGGTGCTGCGCCTGCCGCAG
GGCTACGACACGCGCCTTGGCGACGGCGGCGCCGGACTCTCCGGCGGCCAGCGCCAGCGCATCGGCCTGGCC
CGCGCGCTGTACGGCCGACCGGCGCTGATCGTGCTCGACGAACCCAATGCCAGCCTCGACGAGGCGGGTGAG
GCGGCCCTGGCCGAGGCCATCGCGGCGATGCGCCGGCACGGCAGCAGCCTGGTGCTGGTCACCCACAAGCCG
GCGGTGCTGGCGCTGACCGACAAGCTGTTGTTGCTGCATGGCGGGCGCCTGCAGCGTTTCGGCGCGACCGCC
GAGGTGCTTGCCAGCGCCTCGCCGCCGGCCGCTGCGGCCCCGGCGCCGACACGGCCGACGCCGGCGTTCGGT
TTCGGCTACCGGCCGGGACCGGGGATGCCGGTCGGCGGTGTGCCGGGAGGAGCGGCGCGATGA
Translation:
MKLRPASPRNEILATLGRFRPALRSVALFTAVINLLMLAPSLYMLQVYDRVLGSGNHMTLLMLTLMVLGLYL
LLGALEWVRSLVVIRLGGQLDMQLNQRIYDASFRASLERGEQAAGQALNDLTSLRQFLTGNALFAFFDAPWF
PLYLLVIFLFSPWLGLLALAGALLLVLLAWVNESRSREPLAEAGQLSILATQQASANLRQAETLAAMGMLPA
MRARWFAQHQAFLARQNLGSERSAAIGATSKGVRLALQSLVLGLGAWLAVEGLITPGMMIAGSILMGRVLSP
IDQLIAVWRQWSGARQAYQRLARLLEENPPAALGMPLPVPRGALRVERLCAAAPGREQALLQDLGFALEPGE
ALGVIGPSGSGKSTLARLLVGAWQPLSGAVRLDGADLRQWSAAALGPHIGYLAQDVQLFAGSIAENIARFAE
VDAEKVVAAARLAGVHDLVLRLPQGYDTRLGDGGAGLSGGQRQRIGLARALYGRPALIVLDEPNASLDEAGE
AALAEAIAAMRRHGSSLVLVTHKPAVLALTDKLLLLHGGRLQRFGATAEVLASASPPAAAAPAPTRPTPAFG
FGYRPGPGMPVGGVPGGAAR*
AnnotationID:556GeneID:547
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 11:11:57
Gene Name:hasD
Confidence Code:2
GeneProduct:transport protein HasD
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:ATP-binding metalloprotease secretion protein
Functional Category Confidence Code:5
COGs:COG4618,COG2274,COG1132
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA88492.1| (AB023289) ABC protein [Pseudomonas fluorescens]
          Length = 580

 Score =  770 bits (1989), Expect = 0.0
 Identities = 396/575 (68%), Positives = 461/575 (80%), Gaps = 3/575 (0%)

>dbj|BAA12015.1| (D83582) metalloprotease transporter [Serratia marcescens]
          Length = 582

 Score =  712 bits (1839), Expect = 0.0
 Identities = 367/569 (64%), Positives = 433/569 (76%), Gaps = 2/569 (0%)

>emb|CAD13436.1| (AJ421517) putative ATP-binding metalloprotease secretion protein
           [Yersinia ruckeri]
          Length = 580

 Score =  657 bits (1694), Expect = 0.0
 Identities = 338/573 (58%), Positives = 421/573 (73%)








 
Structural Features:
COG4618, ArpD, ABC-type protease/lipase transport system, ATPase 
and permease components
CD-Length = 580 residues,  99.1% aligned
Score =  742 bits (1918), Expect = 0.0
Identities = 341/575 (59%), Positives = 413/575 (71%), Gaps = 2/575 (0%)

COG2274, SunT, ABC-type bacteriocin/lantibiotic exporters
CD-Length = 709 residues,  79.1% aligned
Score =  349 bits (897), Expect = 6e-97
Identities = 180/566 (31%), Positives = 302/566 (53%), Gaps = 17/566 (3%)

COG1132, MdlB, ABC-type multidrug transport system, ATPase 
and permease components 
[Defense mechanisms]
CD-Length = 567 residues,  96.5% aligned
Score =  263 bits (672), Expect = 6e-71
Identities = 162/552 (29%), Positives = 276/552 (50%), Gaps = 12/552 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1097




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18239




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32157




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:547_cdd.html


    		




 
URL:

















    ReferenceID:34069




  
Author/Investigator(s):
        Idei A, Kawai E, Akatsuka H, Omori K.





  
Title:
        Cloning and characterization of the Pseudomonas fluorescens ATP-binding cassette exporter, HasDEF, for the heme acquisition protein HasA.




  
PubMed:
        10601212


MedLine:
        




    
Source:
J Bacteriol. 1999 Dec;181(24):7545-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10601212









           
Homologs By Global Alignment 

           

       



     
Gene ID:547
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
947
                           PA3406_tr
                           translation of PA3406
                           600
                           98.83
                           99.00
                           4
                           2952.0
     

     
     
38069
                           gb|AAG06794.1|AE004761_10
                           (AE004761) transport protein HasD [Pseudomonas aeruginosa PAO1]
                           600
                           98.83
                           99.00
                           4
                           2952.0
     

     
     
38070
                           dbj|BAA88492.1|
                           (AB023289) ABC protein [Pseudomonas fluorescens]
                           596
                           66.77
                           77.85
                           16
                           1988.0
     

     
     
38073
                           emb|CAA45855.1|
                           (X64558) aprD [Pseudomonas aeruginosa]
                           604
                           60.26
                           72.68
                           19
                           1753.0
     

     
     
38072
                           gb|AAG04635.1|AE004554_3
                           (AE004554) alkaline protease secretion protein AprD [Pseudomonas aeruginosa PAO1]
                           604
                           60.26
                           72.68
                           19
                           1753.0
     

     






      

          
GC ORFID: 43549How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3406Glimmer Score: 

          
Start: 3228399Stop: 3226609

          
Length: 1791


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3406 translation of PA3406Homolog Bit Score 1140.0

          
Other Homologs:  t_PA1246 (ORF: BLASTX 680.0), psyr_15may02_Scaffold17_revised_gene2827 (ORF: BLASTX 664.0), psyr_15may02_Scaffold3_revised_gene4284 (ORF: BLASTX 523.0), t_PA3406 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCTCCGGCCGGCCTCGCCGCGCAATGAAATCCTCGCCACCCTCGGCCGTTTCCGCCCGGCGCTGCGC 
AGCGTCGCGCTGTTCACCGCGGTGATCAACCTGCTGATGCTGGCGCCGTCGCTGTACATGCTGCAGGTCTAC 
GACCGGGTGCTCGGCTCCGGCAACCACATGACCCTGCTGATGCTGACCCTCATGGTGCTCGGCCTCTACCTG 
CTGCTCGGCGCCCTGGAGTGGGTGCGCAGCCTGGTGGTGATCCGCCTCGGCGGCCAGCTCGACATGCAACTC 
AACCAGCGCATCTACGACGCCTCGTTCCGCGCCAGCCTGGAGCGCGGCGAGCAGGCCGCCGGGCAGGCGCTG 
AACGACCTGACCAGCCTGCGCCAGTTCCTCACCGGCAACGCCCTGTTCGCCTTCTTCGACGCGCCCTGGTTC 
CCGCTCTACCTGCTGGTGATCTTCCTCTTCAGTCCCTGGCTCGGCCTGCTCGCCCTGGCCGGGGCACTGCTA 
CTGGTGCTGCTCGCCTGGGTCAACGAAAGCCGCTCGCGCGAACCGCTGGCCGAGGCCGGGCAACTGTCGATC 
CTCGCCACCCAGCAGGCCTCGGCCAACCTGCGCCAGGCGGAAACCCTGGCGGCAATGGGCATGTTGCCGGCC 
ATGCGCGCGCGCTGGTTCGCCCAGCACCAGGCCTTCCTTGCCCGGCAGAACCTCGGCAGCGAGCGCAGCGCG 
GCGATCGGCGCGACCTCCAAGGGCGTGCGCCTGGCCTTGCAGTCGCTGGTGCTCGGCCTCGGCGCCTGGCTG 
GCGGTGGAAGGCCTGATCACCCCGGGGATGATGATCGCCGGCTCGATCCTGATGGGCCGCGTGCTCAGCCCC 
ATCGACCAGTTGATCGCGGTCTGGCGCCAGTGGAGCGGCGCGCGGCAGGCCTACCAGCGACTGGCGCGGCTG 
CTCGAGGAGAATCCGCCTGCCGCCCTGGGCATGCCGCTGCCGGTGCCGCGCGGCGCGTTGCGCGTCGAGCGG 
CTCTGCGCGGCCGCGCCGGGACGCGAGCAGGCATTGTTGCAGGACCTCGGTTTCGCCCTGGAGCCTGGCGAA 
GCGCTCGGCGTGATCGGGCCGTCCGGTTCCGGCAAGTCGACCCTGGCGCGCTTGCTGGTGGGCGCCTGGCAG 
CCGCTGTCCGGCGCGGTGCGCCTGGACGGCGCCGACCTGCGGCAGTGGAGTGCGGCGGCACTGGGGCCGCAC 
ATCGGCTACCTGGCCCAGGACGTGCAACTGTTCGCCGGCAGCATCGCCGAGAACATCGCGCGCTTCGCCGAG 
GTGGATGCCGAGAAGGTGGTGGCGGCGGCGCGCCTGGCCGGCGTGCACGACCTGGTGCTGCGCCTGCCGCAG 
GGCTACGACACGCGCCTTGGCGACGGCGGCGCCGGACTCTCCGGCGGCCAGCGCCAGCGCATCGGCCTGGCC 
CGCGCGCTGTACGGCCGACCGGCGCTGATCGTGCTCGACGAACCCAATGCCAGCCTCGACGAGGCGGGTGAG 
GCGGCCCTGGCCGAGGCCATCGCGGCGATGCGCCGGCACGGCAGCAGCCTGGTGCTGGTCACCCACAAGCCG 
GCGGTGCTGGCGCTGACCGACAAGCTGTTGTTGCTGCATGGCGGGCGCCTGCAGCGTTTCGGCGCGACCGCC 
GAGGTGCTTGCCAGCGCCTCGCCGCCGGCCGCTGCGGCCCCGGCGCCGACACGGCCGACGCCGGCGTTCGGT 
TTCGGCTACCGGCCGGGACCGGGGATGCCGGTCGGCGGTGTGCCGGGAGGAGCGGCGCGATGA