ViewGene
Locus Name:
GeneID:
Location:

GeneID:546
TrivialName: PA14_64390
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64390
PAO1 Ortholog Locus:PA4868
Sequence Length:1701
Protein Length:566
Start: 5735485
Stop: 5737185
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08253.1|AE004900_7 (AE004900) urease alpha subunit [Pseudomonas aeruginosa PAO1]
Identities = 563/566 (99%), Positives = 566/566 (100%)
Sequence:
ATGAAAATCAGCAGACAAGCCTACGCCGACATGTTCGGCCCCACCGTCGGCGACCGCGTGCGCCTGGCCGAC
ACCAACCTGTGGCTCGAGGTGGAACGGGACTTCACCGTCTATGGCGAGGAAGTGAAGTTCGGCGGCGGCAAG
GTCATCCGCGACGGCATGGGCCAGAGCCAGCTGGGCGCGGCGCAGGTGGTCGACACGGTGATCACCAACGCG
CTGATCCTCGACCACTGGGGCGTGGTCAAGGCCGACGTCGGTCTCAAGGACGGGCGCATCCAGGCCATCGGC
AAGGCCGGCAACCCGGACATCCAGCCCGGGGTGAACATCGCCATCGGCGCCGGCACCGAGGTGATCGCCGGC
GAGGGCATGATCCTCACCGCCGGCGGCATCGACACGCACATCCACTTCATCTGCCCGCAGCAGATCGAAGAG
GCGCTGATGAGCGGCGTCACCACCATGATCGGCGGCGGCACCGGCCCCGCCGCCGGGACCAACGCCACCACC
TGCACCTCCGGTCCCTGGCACATGGCGCGGATGCTCCAGGCCGCCGACGCCTTCCCGATGAACATCGGCTTC
ACCGGCAAGGGCAACGCCAGCCTGCCGCTGCCGCTGGAGGAGCAGGTACTCGCCGGCGCCATCGGCCTGAAG
CTGCACGAGGACTGGGGCAGCACCCCGGCGGCGATCGACAACTGCCTGGAGGTGGCCGAGCGCCACGACATC
CAGGTGGCGATCCACACCGACACCCTCAACGAATCCGGCTTCGTCGAGACCACCCTCGGCGCCTTCAAGGGC
CGCACCATCCACACCTACCACACCGAGGGCGCCGGCGGGGGCCACGCACCGGACATCATCAAGGCCTGCGGC
TTCGCCAACGTGCTGCCCAGCTCGACCAACCCGACACGGCCGTTCACCCGCAATACCATCGACGAGCACCTG
GACATGCTGATGGTCTGCCACCACCTCGACCCGGCCATCGCCGAGGACGTGGCCTTCGCCGAGTCGCGCATC
CGCCGCGAGACCATCGCCGCCGAGGACATCCTCCACGACCTCGGCGCGTTCAGCATGATCAGCTCCGACAGC
CAGGCCATGGGCCGGGTCGGCGAAGTGATCACGCGCACCTGGCAGACCGCCGACAAGATGAAGCGCCAGCGC
GGCCGCCTCGACGGCGACGGTGCGCGCAACGACAACTTCCGCGCCAGGCGCTACATCGCCAAGTACACCATC
AACCCGGCGATCACCCACGGCATCAGCCATGAAGTGGGCTCCGTCGAAGCCGGCAAGTGGGCCGACCTGGTG
CTCTGGCGCCCGGCCTTCTTCGGCGTCAAGCCGAGCCTGATCCTCAAGGGCGGGGCCATCGCCGCCAGCCTG
ATGGGCGACATCAACGGCTCGATCCCCACGCCGCAGCCGGTGCACTACCGGCCGATGTTCGCCAGCTACGCC
GGCAGCCGCCACGCGACCAGCCTGACCTTCGTCAGCCAGGCCGCCTTCGCCGCCGGCGTACCGCAGCAGCTC
GGCCTGCGCAAGGCCATCGGCGTGGTCAGCGGCTGTCGCGGTGTGCAGAAGTCCGACCTGATCCACAACGGC
TACCTGCCGACCATCGAGGTCGACGCACAGAACTACCAGGTACGCGCCGACGGCCAGTTGCTCTGGTGCGAA
CCGGCGGACGTGCTGCCGATGGCGCAGCGCTATTTCCTGTTCTGA
Translation:
MKISRQAYADMFGPTVGDRVRLADTNLWLEVERDFTVYGEEVKFGGGKVIRDGMGQSQLGAAQVVDTVITNA
LILDHWGVVKADVGLKDGRIQAIGKAGNPDIQPGVNIAIGAGTEVIAGEGMILTAGGIDTHIHFICPQQIEE
ALMSGVTTMIGGGTGPAAGTNATTCTSGPWHMARMLQAADAFPMNIGFTGKGNASLPLPLEEQVLAGAIGLK
LHEDWGSTPAAIDNCLEVAERHDIQVAIHTDTLNESGFVETTLGAFKGRTIHTYHTEGAGGGHAPDIIKACG
FANVLPSSTNPTRPFTRNTIDEHLDMLMVCHHLDPAIAEDVAFAESRIRRETIAAEDILHDLGAFSMISSDS
QAMGRVGEVITRTWQTADKMKRQRGRLDGDGARNDNFRARRYIAKYTINPAITHGISHEVGSVEAGKWADLV
LWRPAFFGVKPSLILKGGAIAASLMGDINGSIPTPQPVHYRPMFASYAGSRHATSLTFVSQAAFAAGVPQQL
GLRKAIGVVSGCRGVQKSDLIHNGYLPTIEVDAQNYQVRADGQLLWCEPADVLPMAQRYFLF*
AnnotationID:555GeneID:546
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 06:06:57
Gene Name:ureC
Confidence Code:2
GeneProduct:urease alpha subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0804, cd00375
Secondary Functional Category(ies):
EC Number:3.5.1.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58324.1| (AE016873) urease, alpha subunit [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 566

 Score = 1014 bits (2622), Expect = 0.0
 Identities = 494/566 (87%), Positives = 521/566 (92%)

>emb|CAA74065.1| (Y13732) urease, structural subunit [Ralstonia eutropha]
          Length = 570

 Score =  907 bits (2345), Expect = 0.0
 Identities = 451/569 (79%), Positives = 492/569 (86%), Gaps = 5/569 (0%)

>gb|AAN68453.1|AE016478_5 (AE016784) urease, alpha subunit [Pseudomonas putida KT2440]
          Length = 567

 Score =  887 bits (2293), Expect = 0.0
 Identities = 427/564 (75%), Positives = 482/564 (85%)








 
Structural Features:
COG0804, UreC, Urea amidohydrolase (urease) alpha subunit 
[Amino acid transport and metabolism]
CD-Length = 568 residues,  99.8% aligned
Score = 1062 bits (2747), Expect = 0.0
Identities = 440/567 (77%), Positives = 479/567 (84%), Gaps = 1/567 (0%)

cd00375, Urease_alpha, Urease alpha-subunit
CD-Length = 567 residues, 100.0% aligned
Score =  991 bits (2563), Expect = 0.0







   
Genomic Context:






   
Comment:


















    ReferenceID:1095




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18237




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35192




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:546_cdd.html


    		




 
URL:

















    ReferenceID:35954




  
Author/Investigator(s):
        Maeda M, Hidaka M, Nakamura A, Masaki H, Uozumi T.





  
Title:
        Cloning, sequencing, and expression of thermophilic Bacillus sp. strain TB-90 urease gene complex in Escherichia coli.




  
PubMed:
        8288539


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(2):432-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8288539

















    ReferenceID:35958




  
Author/Investigator(s):
        Jones BD, Mobley HL.





  
Title:
        Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease.




  
PubMed:
        2687233


MedLine:
        




    
Source:
J Bacteriol. 1989 Dec;171(12):6414-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2687233

















    ReferenceID:35962




  
Author/Investigator(s):
        Mulrooney SB, Hausinger RP.





  
Title:
        Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.




  
PubMed:
        2211515


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5837-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2211515









           
Homologs By Global Alignment 

           

       



     
Gene ID:546
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
946
                           PA4868_tr
                           translation of PA4868
                           566
                           99.46
                           99.99
                           0
                           2953.0
     

     
     
37827
                           gb|AAG08253.1|AE004900_7
                           (AE004900) urease alpha subunit [Pseudomonas aeruginosa PAO1]
                           566
                           99.46
                           99.99
                           0
                           2953.0
     

     
     
37828
                           gb|AAO58324.1|
                           (AE016873) urease, alpha subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           566
                           87.27
                           92.04
                           0
                           2622.0
     

     
     
37829
                           emb|CAA74065.1|
                           (Y13732) urease, structural subunit [Ralstonia eutropha]
                           571
                           78.98
                           86.16
                           6
                           2351.0
     

     
     
37830
                           emb|CAD15734.1|
                           (AL646067) PROBABLE UREASE (ALPHA SUBUNIT) PROTEIN [Ralstonia solanacearum]
                           572
                           77.62
                           86.36
                           6
                           2343.0
     

     






      

          
GC ORFID: 43548How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4868Glimmer Score: 

          
Start: 4999166Stop: 5000866

          
Length: 1701


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4868 translation of PA4868Homolog Bit Score 1142.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5809 (ORF: BLASTX 812.0), psyr_15may02_Scaffold6_revised_gene6566 (ORF: BLASTX 610.0), psyr_15may02_Scaffold4_revised_gene5808 (ORF: BLASTX 187.0), t_PA4868 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAATCAGCAGACAAGCCTACGCCGACATGTTCGGCCCCACCGTCGGCGACCGCGTGCGCCTGGCCGAC 
ACCAACCTGTGGCTCGAGGTGGAACGGGACTTCACCGTCTATGGCGAGGAAGTGAAGTTCGGCGGCGGCAAG 
GTCATCCGCGACGGCATGGGCCAGAGCCAGCTGGGCGCGGCGCAGGTGGTCGACACGGTGATCACCAACGCG 
CTGATCCTCGACCACTGGGGCGTGGTCAAGGCCGACGTCGGTCTCAAGGACGGGCGCATCCAGGCCATCGGC 
AAGGCCGGCAACCCGGACATCCAGCCCGGGGTGAACATCGCCATCGGCGCCGGCACCGAGGTGATCGCCGGC 
GAGGGCATGATCCTCACCGCCGGCGGCATCGACACGCACATCCACTTCATCTGCCCGCAGCAGATCGAAGAG 
GCGCTGATGAGCGGCGTCACCACCATGATCGGCGGCGGCACCGGCCCCGCCGCCGGGACCAACGCCACCACC 
TGCACCTCCGGTCCCTGGCACATGGCGCGGATGCTCCAGGCCGCCGACGCCTTCCCGATGAACATCGGCTTC 
ACCGGCAAGGGCAACGCCAGCCTGCCGCTGCCGCTGGAGGAGCAGGTACTCGCCGGCGCCATCGGCCTGAAG 
CTGCACGAGGACTGGGGCAGCACCCCGGCGGCGATCGACAACTGCCTGGAGGTGGCCGAGCGCCACGACATC 
CAGGTGGCGATCCACACCGACACCCTCAACGAATCCGGCTTCGTCGAGACCACCCTCGGCGCCTTCAAGGGC 
CGCACCATCCACACCTACCACACCGAGGGCGCCGGCGGGGGCCACGCACCGGACATCATCAAGGCCTGCGGC 
TTCGCCAACGTGCTGCCCAGCTCGACCAACCCGACACGGCCGTTCACCCGCAATACCATCGACGAGCACCTG 
GACATGCTGATGGTCTGCCACCACCTCGACCCGGCCATCGCCGAGGACGTGGCCTTCGCCGAGTCGCGCATC 
CGCCGCGAGACCATCGCCGCCGAGGACATCCTCCACGACCTCGGCGCGTTCAGCATGATCAGCTCCGACAGC 
CAGGCCATGGGCCGGGTCGGCGAAGTGATCACGCGCACCTGGCAGACCGCCGACAAGATGAAGCGCCAGCGC 
GGCCGCCTCGACGGCGACGGTGCGCGCAACGACAACTTCCGCGCCAGGCGCTACATCGCCAAGTACACCATC 
AACCCGGCGATCACCCACGGCATCAGCCATGAAGTGGGCTCCGTCGAAGCCGGCAAGTGGGCCGACCTGGTG 
CTCTGGCGCCCGGCCTTCTTCGGCGTCAAGCCGAGCCTGATCCTCAAGGGCGGGGCCATCGCCGCCAGCCTG 
ATGGGCGACATCAACGGCTCGATCCCCACGCCGCAGCCGGTGCACTACCGGCCGATGTTCGCCAGCTACGCC 
GGCAGCCGCCACGCGACCAGCCTGACCTTCGTCAGCCAGGCCGCCTTCGCCGCCGGCGTACCGCAGCAGCTC 
GGCCTGCGCAAGGCCATCGGCGTGGTCAGCGGCTGTCGCGGTGTGCAGAAGTCCGACCTGATCCACAACGGC 
TACCTGCCGACCATCGAGGTCGACGCACAGAACTACCAGGTACGCGCCGACGGCCAGTTGCTCTGGTGCGAA 
CCGGCGGACGTGCTGCCGATGGCGCAGCGCTATTTCCTGTTCTGA