ViewGene
Locus Name:
GeneID:
Location:

GeneID:544
TrivialName: PA14_66820
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66820
PAO1 Ortholog Locus:PA5056
Sequence Length:1680
Protein Length:559
Start: 5966750
Stop: 5968429
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08441.1|AE004919_2 (AE004919) poly(3-hydroxyalkanoic acid) synthase 1 [Pseudomonas aeruginosa PAO1]
Identities = 558/559 (99%), Positives = 558/559 (99%)
Sequence:
ATGAGTCAAAAGAACAATAACGAGCTTCCCAAGCAAGCCGCGGAAAACACGCTGAACCTGAATCCGGTGATC
GGCATCCGGGGCAAGGACCTGCTCACCTCCGCGCGCATGGTACTGCTCCAGGCGGTGCGCCAGCCACTGCAC
AGCGCCAGGCACGTGGCGCATTTCAGCCTGGAGCTGAAGAACGTCCTGCTCGGCCAGTCGGAGCTGCGCCCA
GGCGACGACGACCGGCGCTTTTCCGACCCGGCCTGGAGCCAGAATCCGCTGTACAAGCGCTACATGCAGACC
TACCTGGCCTGGCGCAAGGAACTGCACAGCTGGATCAGCCACAGCGACCTGTCGCCGCAAGACATCAGTCGT
GGCCAGTTCGTCATCAACCTGCTGACCGAGGCGATGTCGCCGACCAACAGCCTGAGCAACCCGGCGGCGGTC
AAGCGCTTCTTCGAGACCGGCGGCAAGAGCCTGCTGGACGGCCTCGGCCACCTGGCCAAGGACCTGGTGAAC
AACGGCGGGATGCCGAGCCAGGTGGACATGGACGCCTTCGAGGTGGGCAAGAACCTGGCCACCACCGAGGGC
GCCGTGGTGTTCCGCAACGACGTGCTGGAACTGATCCAGTACCGGCCGATCACCGAGTCGGTGCACGAACGC
CCGCTGCTGGTGGTGCCGCCGCAGATCAACAAGTTCTACGTCTTCGACCTGTCGCCGGACAAGAGCCTGGCG
CGCTTCTGCCTGCGCAACGGCGTGCAGACCTTCATCGTCAGCTGGCGCAACCCGACCAAGTCGCAGCGCGAA
TGGGGCCTGACCACCTATATCGAGGCGCTCAAGGAGGCCATCGAGGTAGTCCTGTCGATCACCGGCAGCAAG
GACCTCAACCTCCTCGGCGCCTGCTCCGGCGGGATCACCACCGCGACCCTGGTCGGCCACTACGTCGCCAGC
GGCGAGAAGAAGGTCAACGCCTTCACCCAACTGGTCAGCGTGCTCGACTTCGAACTGAATACCCAGGTCGCG
CTGTTCGCCGACGAGAAGACCCTGGAGGCCGCCAAGCGTCGCTCCTACCAGTCCGGCGTGCTGGAGGGCAAG
GACATGGCCAAGGTGTTCGCCTGGATGCGCCCCAACGACTTGATCTGGAACTACTGGGTCAACAACTACCTG
CTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTG
CACGGcGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCggccccctggaggTCTCCGGCACG
CCCATCGACCTGAAGCaggtgACTTGCGACTTCTactgTGTCGCCGGTCTGAACGACCACATCACCCCCTGG
GAGTCGTGCtacAAGTCGGCCAGGCTACTGGGCGGCAAGTGCgAGTTCATCCTCTCCAACAGCGGCCACATC
CAGAGCATCCTCAACCCACCGGGCAACCCCAAGGCACGCTTCATGACCAATCCGGAACTGCCCGCCGAGCCC
AAGGCCTGGCTGGAACAGGCCGGCAAGCACGCCGATTCGTGGTGGCTGCACTGGCAGCAATGGCTGGCCGAA
CGCTCCGGCAAGACCCGCAAGGCGCCCGCCAGCCTGGGCAACAAGACCTATCCGGCCGGCGAAGCCGCGCCC
GGAACCTACGTGCATGAACGATGA
Translation:
MSQKNNNELPKQAAENTLNLNPVIGIRGKDLLTSARMVLLQAVRQPLHSARHVAHFSLELKNVLLGQSELRP
GDDDRRFSDPAWSQNPLYKRYMQTYLAWRKELHSWISHSDLSPQDISRGQFVINLLTEAMSPTNSLSNPAAV
KRFFETGGKSLLDGLGHLAKDLVNNGGMPSQVDMDAFEVGKNLATTEGAVVFRNDVLELIQYRPITESVHER
PLLVVPPQINKFYVFDLSPDKSLARFCLRNGVQTFIVSWRNPTKSQREWGLTTYIEALKEAIEVVLSITGSK
DLNLLGACSGGITTATLVGHYVASGEKKVNAFTQLVSVLDFELNTQVALFADEKTLEAAKRRSYQSGVLEGK
DMAKVFAWMRPNDLIWNYWVNNYLLGNQPPAFDILYWNNDTTRLPAALHGEFVELFKSNPLNRPGPLEVSGT
PIDLKQVTCDFYCVAGLNDHITPWESCYKSARLLGGKCEFILSNSGHIQSILNPPGNPKARFMTNPELPAEP
KAWLEQAGKHADSWWLHWQQWLAERSGKTRKAPASLGNKTYPAGEAAPGTYVHER*
AnnotationID:553GeneID:544
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 11:11:49
Gene Name:phaC1
Confidence Code:1
GeneProduct:poly(3-hydroxyalkanoic acid) synthase 1
Cell Localization:()
Synonyms:phaC,phaA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:PHA synthase 1
Functional Category Confidence Code:5
COGs:COG3243,pfam07167
Secondary Functional Category(ies):lipid metabolism
EC Number:2.3.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO59383.1| (AY278219) PHA synthase 1 [Pseudomonas stutzeri]
          Length = 559

 Score =  988 bits (2553), Expect = 0.0
 Identities = 463/559 (82%), Positives = 515/559 (92%)

>gb|AAD26365.2|AF129396_1 (AF129396) PHA synthase 1; PHA polymerase 1 [Pseudomonas
           resinovorans]
          Length = 559

 Score =  984 bits (2545), Expect = 0.0
 Identities = 465/559 (83%), Positives = 516/559 (92%)

>gb|AAK81717.1| (AF394660) PHA synthase 1 [Burkholderia caryophylli]
          Length = 559

 Score =  911 bits (2355), Expect = 0.0
 Identities = 433/559 (77%), Positives = 488/559 (87%)








 
Structural Features:
COG3243, PhaC, Poly(3-hydroxyalkanoate) synthetase [Lipid metabolism]
CD-Length = 445 residues, 100.0% aligned
Score =  567 bits (1463), Expect = 1e-162
Identities = 231/455 (50%), Positives = 292/455 (64%), Gaps = 13/455 (2%)

pfam07167, PhaC_N, Poly-beta-hydroxybutyrate polymerase (PhaC) N-terminus.
CD-Length = 172 residues,  99.4% aligned
Score =  257 bits (657), Expect = 3e-69
Identities = 89/171 (52%), Positives = 117/171 (68%), Gaps = 2/171 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1091




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18233




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35013




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:544_cdd.html


    		




 
URL:

















    ReferenceID:35830




  
Author/Investigator(s):
        Eur J Biochem. 1992 Oct 1;209(1):15-30.





  
Title:
        Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1.




  
PubMed:
        1396693


MedLine:
        




    
Source:
Eur J Biochem. 1992 Oct 1;209(1):15-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=93011120&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:544
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
943
                           PA5056_tr
                           translation of PA5056
                           559
                           99.82
                           99.82
                           0
                           2956.0
     

     
     
37783
                           gb|AAG08441.1|AE004919_2
                           (AE004919) poly(3-hydroxyalkanoic acid) synthase 1 [Pseudomonas aeruginosa PAO1]
                           559
                           99.82
                           99.82
                           0
                           2956.0
     

     
     
37784
                           emb|CAA47151.1|
                           (X66592) PHA-synthase1 [Pseudomonas aeruginosa]
                           559
                           99.82
                           99.82
                           0
                           2956.0
     

     
     
37785
                           gb|AAQ72540.1|
                           (AY370934) MCL-PHA synthase 1 [Pseudomonas sp. HJ-2]
                           559
                           86.94
                           94.45
                           0
                           2650.0
     

     
     
37787
                           gb|AAD26365.2|AF129396_1
                           (AF129396) PHA synthase 1; PHA polymerase 1 [Pseudomonas resinovorans]
                           559
                           83.18
                           92.30
                           0
                           2545.0
     

     






      

          
GC ORFID: 43546How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5056Glimmer Score: 

          
Start: 5230433Stop: 5232112

          
Length: 1680


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5056 translation of PA5056Homolog Bit Score 1143.0

          
Other Homologs:  t_PA5058 (ORF: BLASTX 688.0), t_PA5056 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTCAAAAGAACAATAACGAGCTTCCCAAGCAAGCCGCGGAAAACACGCTGAACCTGAATCCGGTGATC 
GGCATCCGGGGCAAGGACCTGCTCACCTCCGCGCGCATGGTACTGCTCCAGGCGGTGCGCCAGCCACTGCAC 
AGCGCCAGGCACGTGGCGCATTTCAGCCTGGAGCTGAAGAACGTCCTGCTCGGCCAGTCGGAGCTGCGCCCA 
GGCGACGACGACCGGCGCTTTTCCGACCCGGCCTGGAGCCAGAATCCGCTGTACAAGCGCTACATGCAGACC 
TACCTGGCCTGGCGCAAGGAACTGCACAGCTGGATCAGCCACAGCGACCTGTCGCCGCAAGACATCAGTCGT 
GGCCAGTTCGTCATCAACCTGCTGACCGAGGCGATGTCGCCGACCAACAGCCTGAGCAACCCGGCGGCGGTC 
AAGCGCTTCTTCGAGACCGGCGGCAAGAGCCTGCTGGACGGCCTCGGCCACCTGGCCAAGGACCTGGTGAAC 
AACGGCGGGATGCCGAGCCAGGTGGACATGGACGCCTTCGAGGTGGGCAAGAACCTGGCCACCACCGAGGGC 
GCCGTGGTGTTCCGCAACGACGTGCTGGAACTGATCCAGTACCGGCCGATCACCGAGTCGGTGCACGAACGC 
CCGCTGCTGGTGGTGCCGCCGCAGATCAACAAGTTCTACGTCTTCGACCTGTCGCCGGACAAGAGCCTGGCG 
CGCTTCTGCCTGCGCAACGGCGTGCAGACCTTCATCGTCAGCTGGCGCAACCCGACCAAGTCGCAGCGCGAA 
TGGGGCCTGACCACCTATATCGAGGCGCTCAAGGAGGCCATCGAGGTAGTCCTGTCGATCACCGGCAGCAAG 
GACCTCAACCTCCTCGGCGCCTGCTCCGGCGGGATCACCACCGCGACCCTGGTCGGCCACTACGTCGCCAGC 
GGCGAGAAGAAGGTCAACGCCTTCACCCAACTGGTCAGCGTGCTCGACTTCGAACTGAATACCCAGGTCGCG 
CTGTTCGCCGACGAGAAGACCCTGGAGGCCGCCAAGCGTCGCTCCTACCAGTCCGGCGTGCTGGAGGGCAAG 
GACATGGCCAAGGTGTTCGCCTGGATGCGCCCCAACGACTTGATCTGGAACTACTGGGTCAACAACTACCTG 
CTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTG 
CACGGcGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCggccccctggaggTCTCCGGCACG 
CCCATCGACCTGAAGCaggtgACTTGCGACTTCTactgTGTCGCCGGTCTGAACGACCACATCACCCCCTGG 
GAGTCGTGCtacAAGTCGGCCAGGCTACTGGGCGGCAAGTGCgAGTTCATCCTCTCCAACAGCGGCCACATC 
CAGAGCATCCTCAACCCACCGGGCAACCCCAAGGCACGCTTCATGACCAATCCGGAACTGCCCGCCGAGCCC 
AAGGCCTGGCTGGAACAGGCCGGCAAGCACGCCGATTCGTGGTGGCTGCACTGGCAGCAATGGCTGGCCGAA 
CGCTCCGGCAAGACCCGCAAGGCGCCCGCCAGCCTGGGCAACAAGACCTATCCGGCCGGCGAAGCCGCGCCC 
GGAACCTACGTGCATGAACGATGA