ViewGene
Locus Name:
GeneID:
Location:

GeneID:5433
TrivialName: PA14_04970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04970
PAO1 Ortholog Locus:PA0380
Sequence Length:201
Protein Length:66
Start: 438196
Stop: 438396
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03769.1|AE004475_11 (AE004475) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 66/66 (100%), Positives = 66/66 (100%)
Sequence:
ATGCAGATTCAACTGAACGGCGAGCCCTTCGAGCTGCCCGACGCGCAGAGCGTCGCGGACCTGCTGGCCCGC
CTGGAGCTTGGCGGCCGCCGCGTGGCCGTCGAGCTGAACCTGGATATCGTGCCGCGCAGCCAGCACGCCAGC
ACCGCGCTCAAGGATGGCGACCGCGTGGAGATCGTCCACGCCATCGGCGGCGGCTAG
Translation:
MQIQLNGEPFELPDAQSVADLLARLELGGRRVAVELNLDIVPRSQHASTALKDGDRVEIVHAIGGG*
AnnotationID:5438GeneID:5433
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 06:06:18
Gene Name:
Confidence Code:3
GeneProduct:thiamine biosynthesis protein ThiS
Cell Localization:()
Synonyms:thiS
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2104,pfam02597
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53977.1| (AE016857) thiamine biosynthesis protein ThiS [Pseudomonas
          syringae pv. tomato str. DC3000]
          Length = 66

 Score =  105 bits (261), Expect = 8e-23
 Identities = 50/66 (75%), Positives = 58/66 (87%)






 
Structural Features:
COG2104, ThiS, Sulfur transfer protein involved in thiamine biosynthesis 
[Coenzyme metabolism]
CD-Length = 68 residues,  97.1% aligned
Score = 82.2 bits (203), Expect = 1e-17
Identities = 37/66 (56%), Positives = 48/66 (72%)

pfam02597, ThiS, ThiS family. ThiS (thiaminS) is a 66 aa protein 
involved in sulphur transfer.
CD-Length = 63 residues, 100.0% aligned
Score = 58.0 bits (140), Expect = 2e-10
Identities = 32/65 (49%), Positives = 40/65 (61%), Gaps = 2/65 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:10864




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:28012




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33706




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:5433_cdd.html


    		




 
URL:

















    ReferenceID:35389




  
Author/Investigator(s):
        Kelleher NL, Taylor SV, Grannis D, Kinsland C, Chiu HJ, Begley TP, McLafferty FW.





  
Title:
        Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry.




  
PubMed:
        10082377


MedLine:
        




    
Source:
Protein Sci. 1998 Aug;7(8):1796-801.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10082377









           
Homologs By Global Alignment 

           

       



     
Gene ID:5433
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7826
                           PA0380_tr
                           translation of PA0380
                           66
                           99.99
                           99.99
                           0
                           328.0
     

     
     
196521
                           gb|AAG03769.1|AE004475_11
                           (AE004475) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           66
                           99.99
                           99.99
                           0
                           328.0
     

     
     
196523
                           gb|AAO53977.1|
                           (AE016857) thiamine biosynthesis protein ThiS [Pseudomonas syringae pv. tomato str. DC3000]
                           66
                           75.75
                           87.87
                           0
                           261.0
     

     
     
196522
                           gb|AAN70670.1|AE016710_15
                           (AE016793) conserved hypothetical protein [Pseudomonas putida KT2440]
                           92
                           57.60
                           64.13
                           26
                           265.0
     

     






      

          
GC ORFID: 48435How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0380Glimmer Score: 

          
Start: 6239538Stop: 6239738

          
Length: 201


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA0380 translation of PA0380Homolog Bit Score 130.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6617 (ORF: BLASTX 107.0), t_PA0380 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAGATTCAACTGAACGGCGAGCCCTTCGAGCTGCCCGACGCGCAGAGCGTCGCGGACCTGCTGGCCCGC 
CTGGAGCTTGGCGGCCGCCGCGTGGCCGTCGAGCTGAACCTGGATATCGTGCCGCGCAGCCAGCACGCCAGC 
ACCGCGCTCAAGGATGGCGACCGCGTGGAGATCGTCCACGCCATCGGCGGCGGCTAG