ViewGene
Locus Name:
GeneID:
Location:

GeneID:5422
TrivialName: PA14_60280
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60280
PAO1 Ortholog Locus:
Sequence Length:489
Protein Length:162
Start: 5370692
Stop: 5371180
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAP81272.1| (AY273871) FimU [Pseudomonas aeruginosa]
Identities = 162/162 (100%), Positives = 162/162 (100%). Similar to PA4550 in PAO1, fimU.
Sequence:
ATGCGCTCTATTTGTCGCAGCGCCGGCTTTTCCCTGATCGAGTTGATGATGGTGTTGGTTCTGGTCGCCATA
TTCGCCAGCATTGCCGTACCCAGTTTCAACGCCTTGATCGAGCGCAACCGAATCCAGACTGCCAGCGAGGAA
CTCTACAGCCTGCTTCAGTACGCTCGCAGCGAAGCTGTAAACCGTCATGCCAATGTGAGCATCAGGGCGACG
CAGAACAATGACTGGGCAAAAGGCCTGGAAATCATCAGCGGCGCGACCACCGTGCAAAAGCACCAAGGTTTC
CAGCAGGTCTCGCTATCCGCCAGCAGTGCGACTGCGGAGCTGACCTTCAACGCTACCGGCACACTTAGCAAC
CAGGCTGCAAACATTGACATAAAGGTCTGCTTCGCCGGTGACAAAAGTACAGGACGTCTGCTTACCGTTCAG
CCCAGTGGACGCGTGATCCTGTACCCATCTTCAAAGCAACCGGACAGCTGTAACTGA
Translation:
MRSICRSAGFSLIELMMVLVLVAIFASIAVPSFNALIERNRIQTASEELYSLLQYARSEAVNRHANVSIRAT
QNNDWAKGLEIISGATTVQKHQGFQQVSLSASSATAELTFNATGTLSNQAANIDIKVCFAGDKSTGRLLTVQ
PSGRVILYPSSKQPDSCN*
AnnotationID:5427GeneID:5422
AnnotatorUID: danlee
Modification Date/Time: 2005-10-05 17:05:43
Gene Name:fimU
Confidence Code:1
GeneProduct:type 4 fimbrial biogenesis protein FimU
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:
Pilin biosynthesis
Homology:
gi|11352765|pir||H83075  type 4 fimbrial biogenesis protein FimU PA4550 
[Pseudomonas aeruginosa PAO1]
     Length=168
     Score =  134 bits (338),  Expect = 8e-31
     Identities = 76/162 (46%), Positives = 106/162 (65%), Gaps = 8/162 (4%)
Structural Features:
COG4970, FimT, Tfp pilus assembly protein FimT [Cell motility and secretion / Intracellular 
trafficking and secretion].
             CD-Length = 181 residues, only  41.4% aligned
             Score = 76.2 bits (187), Expect = 2e-15
Genomic Context:
PA14 GeneIDs 5422 to 5476 (Trivial Names PA14_60280 to PA14_60320) replace PAO1 genes 
PA4549 through PA4556 required for type 4 fimbrial biogenesis.  PA14 ORFs corresponding 
to fimT (PA4549), pilV (PA4551), and pilY2 (PA4555) are absent.
Comment:


















    ReferenceID:10842




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:27990




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33007




  
Author/Investigator(s):
        Alm RA, Mattick JS.





  
Title:
        Identification of two genes with prepilin-like leader sequences involved in type 4 fimbrial biogenesis in Pseudomonas aeruginosa.




  
PubMed:
        8682785


MedLine:
        




    
Source:
J Bacteriol. 1996 Jul;178(13):3809-17.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8682785









           
Homologs By Global Alignment 

           

       



     
Gene ID:5422
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
196499
                           gb|AAP81272.1|
                           (AY273871) FimU [Pseudomonas aeruginosa]
                           162
                           99.99
                           99.99
                           0
                           799.0
     

     
     
7815
                           PA4550_tr
                           translation of PA4550
                           169
                           45.56
                           63.31
                           8
                           344.0
     

     
     
196500
                           gb|AAG07938.1|AE004869_2
                           (AE004869) type 4 fimbrial biogenesis protein FimU [Pseudomonas aeruginosa PAO1]
                           169
                           45.56
                           63.31
                           8
                           344.0
     

     
     
196501
                           gb|AAB39271.1|
                           (L48934) contains pre-pilin like leader sequence; involved in type 4 fimbrial biogenesis [Pseudomonas aeruginosa]
                           169
                           45.56
                           63.31
                           8
                           344.0
     

     






      

          
GC ORFID: 48424How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4550Glimmer Score: 

          
Start: 4634371Stop: 4634859

          
Length: 489


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4550 translation of PA4550Homolog Bit Score 134.0

          
Other Homologs:  t_PA4550 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCTCTATTTGTCGCAGCGCCGGCTTTTCCCTGATCGAGTTGATGATGGTGTTGGTTCTGGTCGCCATA 
TTCGCCAGCATTGCCGTACCCAGTTTCAACGCCTTGATCGAGCGCAACCGAATCCAGACTGCCAGCGAGGAA 
CTCTACAGCCTGCTTCAGTACGCTCGCAGCGAAGCTGTAAACCGTCATGCCAATGTGAGCATCAGGGCGACG 
CAGAACAATGACTGGGCAAAAGGCCTGGAAATCATCAGCGGCGCGACCACCGTGCAAAAGCACCAAGGTTTC 
CAGCAGGTCTCGCTATCCGCCAGCAGTGCGACTGCGGAGCTGACCTTCAACGCTACCGGCACACTTAGCAAC 
CAGGCTGCAAACATTGACATAAAGGTCTGCTTCGCCGGTGACAAAAGTACAGGACGTCTGCTTACCGTTCAG 
CCCAGTGGACGCGTGATCCTGTACCCATCTTCAAAGCAACCGGACAGCTGTAACTGA