ViewGene
Locus Name:
GeneID:
Location:

GeneID:532
TrivialName: PA14_41380
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41380
PAO1 Ortholog Locus:PA1794
Sequence Length:1671
Protein Length:556
Start: 3691609
Stop: 3689939
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05183.1|AE004605_5 (AE004605) glutaminyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 554/556 (99%), Positives = 555/556 (99%)
Sequence:
ATGAGCAAGCCAGAGACCACCGCCGCCCCGAACTTCCTGCGCCAGATCGTTCAAGCCGACCTGGATGCCGGC
AAGCACGCGAAGATCGTCACCCGTTTCCCCCCGGAGCCCAACGGTTACCTGCACATCGGCCACGCCAAGTCG
ATCTGCCTGAACTTCGGCCTGGCCCAGGAATTCGCTGGCGATTGCCATCTGCGCTTCGATGACACCAACCCG
GCCAAGGAAGACCAGGAGTACATCGATGCCATCGAGGCCGACATCAAGTGGCTGGGCTTCCAGTGGTCCGGC
GAGGTCTGCTATGCCTCCAACTATTTCGACCAGCTGCATGCCTGGGCCATCGAGCTGATCAAGGCCGGCAAG
GCGTTCGTCTGCGACCTCGGTCCCGAGGAAATGCGCGAATACCGCGGCACCCTCACCGAGCCGGGCCGCAAC
AGCCCTTACCGTGACCGTTCGGTGGAGGAGAACCTCGACCTGTTCGCCCGCATGAAGGCCGGTGAGTTCCCC
GACGGCGCCCGTTCGCTGCGGGCGAAGATCGACATGGGTTCGCCGAACATGAACCTGCGCGACCCGATCCTG
TACCGCATCCGCCATGCGCATCACCACCAGACCGGCGACAAGTGGTGCATCTACCCGAGCTACGACTTCACC
CACGGTCAGTCGGACGCCATCGAGGGCATCACCCATTCGATCTGCACCCTGGAATTCGAAGACCATCGTCCG
CTCTACGAGTGGTTCCTGGCCAACCTGCCGGTGCCTGCGCAACCGCGCCAGTACGAATTCTCCCGGCTCAAC
CTGAACTACACCGTGACCAGCAAGCGCAAGCTCAAGCAACTGGTCGACGAAGGCCATGTCAGCGGCTGGGAC
GATCCGCGCATGTCGACCCTGTCCGGCTATCGCCGCCGCGGCTACACCCCGGAGTCGATCCGCAACTTCTGC
GAGATGATCGGGGTGAACCGCGCCAGCGGCGTGGTCGACATCGGCATGCTCGAGTTCAGCATCCGCGACCAC
CTGGACGCCACCGCGCCGCGTGCCATGTGCGTGCTGAAACCGCTCAAGGTGGTGATCACCAACTACCCCGAA
GGGCAGGTGGAGAACCTCGAGTTGCCGCGTCATCCGAAGGAAGACATGGGGGTGCGCGTACTGCCGTTCGGT
CGCGAGCTGTTCATCGATGCCGGTGACTTCGAGGAAGTCCCGCCGGCCGGCTACAAGCGCCTGATCCCCGGT
GGCGAGGTGCGTCTGCGTGGCAGCTACGTGATCCGTGCCGACGAAGCGATCAAGGATGCCGACGGCAATATC
GTCGAACTGCGCTGCAGCTACGATCCGGACACCCTGGGCAAGAACCCGGAAGGCCGCAAGGTCAAGGGCGTG
ATCCACTGGGTGCCGGCCGAGGGCAGCGTCGAGTGCGAAGTGCGCCTGTACGATCGGCTGTTCCGCTCGGCC
AATCCGGAGAAGGCGGAAGAGGGCGGCAGCTTCCTCGACAACATCAACGCCGACTCCCTGCAGGTACTGACC
GGCTGTCGCGCCGAGCCGTCGCTGGGCCAGGCGAACCCGGAGGATCGCTTCCAGTTCGAGCGCGAAGGCTAT
TTCGTCGCCGACCTGAAGGACTCCAGGCCGGGCAAGCCGGTGTTCAACCGTACCGTCACCCTGCGTGACTCC
TGGGGACAGGGCTGA
Translation:
MSKPETTAAPNFLRQIVQADLDAGKHAKIVTRFPPEPNGYLHIGHAKSICLNFGLAQEFAGDCHLRFDDTNP
AKEDQEYIDAIEADIKWLGFQWSGEVCYASNYFDQLHAWAIELIKAGKAFVCDLGPEEMREYRGTLTEPGRN
SPYRDRSVEENLDLFARMKAGEFPDGARSLRAKIDMGSPNMNLRDPILYRIRHAHHHQTGDKWCIYPSYDFT
HGQSDAIEGITHSICTLEFEDHRPLYEWFLANLPVPAQPRQYEFSRLNLNYTVTSKRKLKQLVDEGHVSGWD
DPRMSTLSGYRRRGYTPESIRNFCEMIGVNRASGVVDIGMLEFSIRDHLDATAPRAMCVLKPLKVVITNYPE
GQVENLELPRHPKEDMGVRVLPFGRELFIDAGDFEEVPPAGYKRLIPGGEVRLRGSYVIRADEAIKDADGNI
VELRCSYDPDTLGKNPEGRKVKGVIHWVPAEGSVECEVRLYDRLFRSANPEKAEEGGSFLDNINADSLQVLT
GCRAEPSLGQANPEDRFQFEREGYFVADLKDSRPGKPVFNRTVTLRDSWGQG*
AnnotationID:541GeneID:532
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 13:01:06
Gene Name:glnS
Confidence Code:2
GeneProduct:glutaminyl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00807,COG0008,cd00418
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:6.1.1.18
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68512.1|AE016483_3 (AE016784) glutaminyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 567

 Score =  999 bits (2584), Expect = 0.0
 Identities = 477/566 (84%), Positives = 506/566 (89%), Gaps = 12/566 (2%)

>gb|AAO57212.1| (AE016869) glutaminyl-tRNA synthetase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 569

 Score =  994 bits (2569), Expect = 0.0
 Identities = 470/552 (85%), Positives = 504/552 (91%)

>gb|AAF41914.1| (AE002506) glutaminyl-tRNA synthetase [Neisseria meningitidis MC58]
          Length = 562

 Score =  717 bits (1850), Expect = 0.0
 Identities = 349/560 (62%), Positives = 428/560 (76%), Gaps = 8/560 (1%)








 
Structural Features:
cd00807, Gln_GluRS_non_core, Glutaminyl-tRNA synthetase (GlnRS) and 
non-descriminating Glutamyl-tRNA synthetase (GluRS) cataytic core domain.
CD-Length = 307 residues,  99.7% aligned
Score =  446 bits (1149), Expect = 3e-126

COG0008, GlnS, Glutamyl- and glutaminyl-tRNA synthetases 
[Translation, ribosomal structure and biogenesis]
CD-Length = 472 residues,  98.5% aligned
Score =  376 bits (967), Expect = 4e-105
Identities = 171/518 (33%), Positives = 218/518 (42%), Gaps = 65/518 (12%)

cd00418, GlxRS_core, Glutamyl-tRNA synthetase(GluRS)/
Glutaminyl-tRNA synthetase (GlnRS) cataytic core domain.
CD-Length = 344 residues,  90.7% aligned
Score =  372 bits (956), Expect = 6e-104







   
Genomic Context:






   
Comment:


















    ReferenceID:1067




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18209




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36517




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:532_cdd.html


    		




 
URL:

















    ReferenceID:36585




  
Author/Investigator(s):
        Yamao F, Inokuchi H, Cheung A, Ozeki H, Soll D.





  
Title:
        Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene.




  
PubMed:
        6288695


MedLine:
        




    
Source:
J Biol Chem. 1982 Oct 10;257(19):11639-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6288695









           
Homologs By Global Alignment 

           

       



     
Gene ID:532
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
929
                           PA1794_tr
                           translation of PA1794
                           556
                           99.64
                           99.82
                           0
                           2987.0
     

     
     
37365
                           gb|AAG05183.1|AE004605_5
                           (AE004605) glutaminyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           556
                           99.64
                           99.82
                           0
                           2987.0
     

     
     
37366
                           gb|AAN68512.1|AE016483_3
                           (AE016784) glutaminyl-tRNA synthetase [Pseudomonas putida KT2440]
                           568
                           83.97
                           89.08
                           13
                           2591.0
     

     
     
37367
                           gb|AAO57212.1|
                           (AE016869) glutaminyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           571
                           83.01
                           89.14
                           17
                           2572.0
     

     
     
37368
                           gb|AAR36756.1|
                           (AE017219) glutaminyl-tRNA synthetase [Geobacter sulfurreducens PCA]
                           559
                           63.68
                           76.74
                           8
                           1923.0
     

     






      

          
GC ORFID: 43534How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1794Glimmer Score: 

          
Start: 1262300Stop: 1263970

          
Length: 1671


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1794 translation of PA1794Homolog Bit Score 1155.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene762 (ORF: BLASTX 996.0), t_PA1794 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAGCCAGAGACCACCGCCGCCCCGAACTTCCTGCGCCAGATCGTTCAAGCCGACCTGGATGCCGGC 
AAGCACGCGAAGATCGTCACCCGTTTCCCCCCGGAGCCCAACGGTTACCTGCACATCGGCCACGCCAAGTCG 
ATCTGCCTGAACTTCGGCCTGGCCCAGGAATTCGCTGGCGATTGCCATCTGCGCTTCGATGACACCAACCCG 
GCCAAGGAAGACCAGGAGTACATCGATGCCATCGAGGCCGACATCAAGTGGCTGGGCTTCCAGTGGTCCGGC 
GAGGTCTGCTATGCCTCCAACTATTTCGACCAGCTGCATGCCTGGGCCATCGAGCTGATCAAGGCCGGCAAG 
GCGTTCGTCTGCGACCTCGGTCCCGAGGAAATGCGCGAATACCGCGGCACCCTCACCGAGCCGGGCCGCAAC 
AGCCCTTACCGTGACCGTTCGGTGGAGGAGAACCTCGACCTGTTCGCCCGCATGAAGGCCGGTGAGTTCCCC 
GACGGCGCCCGTTCGCTGCGGGCGAAGATCGACATGGGTTCGCCGAACATGAACCTGCGCGACCCGATCCTG 
TACCGCATCCGCCATGCGCATCACCACCAGACCGGCGACAAGTGGTGCATCTACCCGAGCTACGACTTCACC 
CACGGTCAGTCGGACGCCATCGAGGGCATCACCCATTCGATCTGCACCCTGGAATTCGAAGACCATCGTCCG 
CTCTACGAGTGGTTCCTGGCCAACCTGCCGGTGCCTGCGCAACCGCGCCAGTACGAATTCTCCCGGCTCAAC 
CTGAACTACACCGTGACCAGCAAGCGCAAGCTCAAGCAACTGGTCGACGAAGGCCATGTCAGCGGCTGGGAC 
GATCCGCGCATGTCGACCCTGTCCGGCTATCGCCGCCGCGGCTACACCCCGGAGTCGATCCGCAACTTCTGC 
GAGATGATCGGGGTGAACCGCGCCAGCGGCGTGGTCGACATCGGCATGCTCGAGTTCAGCATCCGCGACCAC 
CTGGACGCCACCGCGCCGCGTGCCATGTGCGTGCTGAAACCGCTCAAGGTGGTGATCACCAACTACCCCGAA 
GGGCAGGTGGAGAACCTCGAGTTGCCGCGTCATCCGAAGGAAGACATGGGGGTGCGCGTACTGCCGTTCGGT 
CGCGAGCTGTTCATCGATGCCGGTGACTTCGAGGAAGTCCCGCCGGCCGGCTACAAGCGCCTGATCCCCGGT 
GGCGAGGTGCGTCTGCGTGGCAGCTACGTGATCCGTGCCGACGAAGCGATCAAGGATGCCGACGGCAATATC 
GTCGAACTGCGCTGCAGCTACGATCCGGACACCCTGGGCAAGAACCCGGAAGGCCGCAAGGTCAAGGGCGTG 
ATCCACTGGGTGCCGGCCGAGGGCAGCGTCGAGTGCGAAGTGCGCCTGTACGATCGGCTGTTCCGCTCGGCC 
AATCCGGAGAAGGCGGAAGAGGGCGGCAGCTTCCTCGACAACATCAACGCCGACTCCCTGCAGGTACTGACC 
GGCTGTCGCGCCGAGCCGTCGCTGGGCCAGGCGAACCCGGAGGATCGCTTCCAGTTCGAGCGCGAAGGCTAT 
TTCGTCGCCGACCTGAAGGACTCCAGGCCGGGCAAGCCGGTGTTCAACCGTACCGTCACCCTGCGTGACTCC 
TGGGGACAGGGCTGA