ViewGene

Locus Name:
GeneID:
Location:

GeneID: 5314

TrivialName: PA14_54910

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_54910

PAO1 Ortholog Locus:

Sequence Length: 816

Protein Length: 271

Start: 4867507

Stop: 4866692

Strand: -

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: dbj|BAC68663.1| (AP005025) thioesterase [Streptomyces avermitilis MA-4680] Identities = 106/247 (42%), Positives = 147/247 (59%), Gaps = 6/247 (2%)

Sequence:

TTGAGCGCAGAAGCCCACGGCGATTTTCCGACATATCGGTCCTGGTTCCGAATTCCGGCCTCCAGGGCTGAT
CCGGCCTGGCGCTTAATCTGCCTGCCCCACGCTGGCGGATCGGCCAGCTTCTTCTATCCCTGGAGTCGAGTG
CTCCTGCCCGAAGTGGAGTTGGTGGCGATCCAGTACCCAGGGCGCGAAGAGCGCATAGGTGAGCCCTGCATC
GATGACATGGACACCATGGTGAGCAGTCTGGCCCAGGCACTCGCCGCTGTTCCGCAGTTGCTGCAACGGCCC
TATGTACTGTTTGGCCACAGCATGGGAGCGGCGGTCGCCTATGAGCTTGTACTGGCCTTGCAGCAACGGAAT
GCTGCGCTGCCCAACCTGCTTGCGCTCTCGGCGAGCGAGGGGCCCGGAGCGGTGAAGCCCAGTGCGCTGCAC
TGTGCCACCGACGGTGCATTGGTGGCCGAAATTGTCCGACTCAACAATCGATTGGAGCACCTGCTGCATTCA
CCGGAACTGACGGGCCTGGTCCTTCCGGCGCTGCGCAGCGACTACCGGCTGATAGAAACCTACGGTGGCAGA
AAGCCCTCGTACAGCCAGGTGCGGGTGCCCGTGGCGGCACTGGTGGGACGGGAAGACAGCGAGCTGAGCCTG
GACGACGCGCAAGCTTGGCAGCGGATCGCTAGCCAGGGCTTCGAGCTATGCAGTTTCCCGGGTGGGCACTTC
TACCTGAGTCAGCAGTTTCCGGCATTGACCGAACAGCTATGCCGACGCCTGACCGAGCACAGCCTGGGAAAG
ACACCGTGGCAAGAACTGCCCTGA

Translation:

MSAEAHGDFPTYRSWFRIPASRADPAWRLICLPHAGGSASFFYPWSRVLLPEVELVAIQYPGREERIGEPCI
DDMDTMVSSLAQALAAVPQLLQRPYVLFGHSMGAAVAYELVLALQQRNAALPNLLALSASEGPGAVKPSALH
CATDGALVAEIVRLNNRLEHLLHSPELTGLVLPALRSDYRLIETYGGRKPSYSQVRVPVAALVGREDSELSL
DDAQAWQRIASQGFELCSFPGGHFYLSQQFPALTEQLCRRLTEHSLGKTPWQELP*

AnnotationID:5319 GeneID:5314

AnnotatorUID: danlee

Modification Date/Time: 2005-10-03 15:03:07

Gene Name:

Confidence Code: 3

GeneProduct: putative thioesterase

Cell Localization: ()

Synonyms:

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (20) Putative enzymes

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: COG3208, pfam00975

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Homology:

gi|46313307|ref|ZP_00213898.1|  COG3208: Predicted thioesterase involved in non-ribosomal 
peptide biosynthesis [Burkholderia cepacia R18194]
     Length=257
     Score =  202 bits (515),  Expect = 7e-51
     Identities = 110/250 (44%), Positives = 151/250 (60%), Gaps = 6/250 (2%)

gi|67102828|gb|EAM19957.1|  Thioesterase [Burkholderia cenocepacia HI2424]
gi|67097476|gb|EAM14998.1|  Thioesterase [Burkholderia cenocepacia AU 1054]
     Length=257
     Score =  197 bits (502),  Expect = 2e-49
     Identities = 106/250 (42%), Positives = 149/250 (59%), Gaps = 6/250 (2%)

 
gi|68345151|gb|AAY92757.1|  pyochelin biosynthetic protein PchC PA4229 [Pseudomonas fluorescens Pf-5] 
     Length=259
     Score =  197 bits (502),  Expect = 2e-49
     Identities = 111/258 (43%), Positives = 152/258 (58%), Gaps = 8/258 (3%)

Structural Features:

COG3208, GrsT, Predicted thioesterase involved in non-ribosomal peptide biosynthesis 
[Secondary metabolites biosynthesis, transport, and catabolism].
             CD-Length = 244 residues,  96.7% aligned
             Score =  173 bits (441), Expect = 1e-44

pfam00975, Thioesterase, Thioesterase domain. Peptide synthetases are involved in the 
non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes, 
in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid 
thioesterases of vertebrates. There are also modules within the peptide synthetases that 
also share this similarity.
             CD-Length = 227 residues,  99.6% aligned
             Score =  120 bits (302), Expect = 2e-28

Genomic Context:

Comment:

ReferenceID:15310

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:27775

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

Homologs By Global Alignment

Gene ID:5314

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

195881 emb|CAC17504.1| (AL939132) putative thioesterase [Streptomyces coelicolor A3(2)] 287 40.41 51.21 26 466.0

195877 dbj|BAC71351.1| (AP005035) putative thioesterase [Streptomyces avermitilis MA-4680] 274 39.41 52.91 28 476.0

195878 dbj|BAB69336.1| (AB070951) thioesterase [Streptomyces avermitilis] 274 39.41 52.91 28 476.0

195876 dbj|BAC68663.1| (AP005025) thioesterase [Streptomyces avermitilis MA-4680] 271 39.11 54.24 21 471.0

195885 emb|CAA57967.1| (X82644) pchC [Pseudomonas aeruginosa] 281 38.07 48.04 41 408.0

GC ORFID: `48316`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA4229`	Glimmer Score:
Start: `86400`	Stop: `87215`
Length: `816`
Start Codon: `TTG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA4229 translation of PA4229`	Homolog Bit Score `165.0`
Other Homologs:
GC ORF Sequence
TTGAGCGCAGAAGCCCACGGCGATTTTCCGACATATCGGTCCTGGTTCCGAATTCCGGCCTCCAGGGCTGAT CCGGCCTGGCGCTTAATCTGCCTGCCCCACGCTGGCGGATCGGCCAGCTTCTTCTATCCCTGGAGTCGAGTG CTCCTGCCCGAAGTGGAGTTGGTGGCGATCCAGTACCCAGGGCGCGAAGAGCGCATAGGTGAGCCCTGCATC GATGACATGGACACCATGGTGAGCAGTCTGGCCCAGGCACTCGCCGCTGTTCCGCAGTTGCTGCAACGGCCC TATGTACTGTTTGGCCACAGCATGGGAGCGGCGGTCGCCTATGAGCTTGTACTGGCCTTGCAGCAACGGAAT GCTGCGCTGCCCAACCTGCTTGCGCTCTCGGCGAGCGAGGGGCCCGGAGCGGTGAAGCCCAGTGCGCTGCAC TGTGCCACCGACGGTGCATTGGTGGCCGAAATTGTCCGACTCAACAATCGATTGGAGCACCTGCTGCATTCA CCGGAACTGACGGGCCTGGTCCTTCCGGCGCTGCGCAGCGACTACCGGCTGATAGAAACCTACGGTGGCAGA AAGCCCTCGTACAGCCAGGTGCGGGTGCCCGTGGCGGCACTGGTGGGACGGGAAGACAGCGAGCTGAGCCTG GACGACGCGCAAGCTTGGCAGCGGATCGCTAGCCAGGGCTTCGAGCTATGCAGTTTCCCGGGTGGGCACTTC TACCTGAGTCAGCAGTTTCCGGCATTGACCGAACAGCTATGCCGACGCCTGACCGAGCACAGCCTGGGAAAG ACACCGTGGCAAGAACTGCCCTGA