ViewGene
Locus Name:
GeneID:
Location:

GeneID:5311
TrivialName: PA14_22010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_22010
PAO1 Ortholog Locus:PA3245
Sequence Length:255
Protein Length:84
Start: 1914799
Stop: 1914545
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06633.1|AE004747_6 (AE004747) cell division topological specificity factor MinE [Pseudomonas aeruginosa PAO1]
Identities = 84/84 (100%), Positives = 84/84 (100%)
Sequence:
ATGAGCCTTTTAGACTTCTTTCGCAGTCGGAAATCGCAGAACAGCGCTTCCATCGCGAAAGAACGACTGCAG
ATCATCGTTGCCCACGAACGTGGCCAGCGCGCACAGCCGGATTATCTGCCCCAGTTGCAGAAAGACCTGCTG
GAAGTGATCCGCAAGTACGTACCGATCGACCAGGAGCAGATCCAGGTGGAACTGGAGAACCAGGGCAACTGC
TCGATCCTGGAACTCAACATCACCCTGCCGGATCGTTGA
Translation:
MSLLDFFRSRKSQNSASIAKERLQIIVAHERGQRAQPDYLPQLQKDLLEVIRKYVPIDQEQIQVELENQGNC
SILELNITLPDR*
AnnotationID:5316GeneID:5311
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 14:02:18
Gene Name:minE
Confidence Code:2
GeneProduct:cell division topological specificity factor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(6) Cell division
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam03776,COG0851
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67352.1|AE016361_6 (AE016780) cell division topological specificity factor MinE
          [Pseudomonas putida KT2440]
          Length = 84

 Score =  134 bits (336), Expect = 1e-31
 Identities = 64/84 (76%), Positives = 74/84 (88%)

>gb|AAO57341.1| (AE016870) cell division topological specificity factor MinE
          [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 84

 Score =  131 bits (330), Expect = 7e-31
 Identities = 62/84 (73%), Positives = 76/84 (90%)

>dbj|BAA36021.1| (D90752) Cell division topological specificity factor.
          [Escherichia coli K12]
          Length = 88

 Score =  100 bits (250), Expect = 1e-21
 Identities = 55/84 (65%), Positives = 68/84 (80%), Gaps = 2/84 (2%)






 
Structural Features:
pfam03776, MinE, Septum formation topological specificity factor MinE.
CD-Length = 82 residues, 100.0% aligned
Score =  109 bits (273), Expect = 6e-26
Identities = 54/83 (65%), Positives = 68/83 (81%), Gaps = 1/83 (1%)

COG0851, MinE, Septum formation topological specificity factor 
[Cell division and chromosome partitioning]
CD-Length = 88 residues,  94.3% aligned
Score =  107 bits (268), Expect = 2e-25
Identities = 53/84 (63%), Positives = 64/84 (76%), Gaps = 1/84 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:10622




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:27769




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32297




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:5311_cdd.html


    		




 
URL:

















    ReferenceID:33985




  
Author/Investigator(s):
        Ma LY, King G, Rothfield L.





  
Title:
        Mapping the MinE site involved in interaction with the MinD division site selection protein of Escherichia coli.




  
PubMed:
        12897015


MedLine:
        




    
Source:
J Bacteriol. 2003 Aug;185(16):4948-55.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12897015

















    ReferenceID:33986




  
Author/Investigator(s):
        Zhao CR, de Boer PA, Rothfield LI.





  
Title:
        Proper placement of the Escherichia coli division site requires two functions that are associated with different domains of the MinE protein.




  
PubMed:
        7753804


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1995 May 9;92(10):4313-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7753804









           
Homologs By Global Alignment 

           

       



     
Gene ID:5311
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7712
                           PA3245_tr
                           translation of PA3245
                           84
                           99.99
                           99.99
                           0
                           419.0
     

     
     
195860
                           gb|AAG06633.1|AE004747_6
                           (AE004747) cell division topological specificity factor MinE [Pseudomonas aeruginosa PAO1]
                           84
                           99.99
                           99.99
                           0
                           419.0
     

     
     
195861
                           gb|AAN67352.1|AE016361_6
                           (AE016780) cell division topological specificity factor MinE [Pseudomonas putida KT2440]
                           84
                           76.19
                           88.09
                           0
                           336.0
     

     
     
195862
                           gb|AAO57341.1|
                           (AE016870) cell division topological specificity factor MinE [Pseudomonas syringae pv. tomato str. DC3000]
                           84
                           73.80
                           90.47
                           0
                           330.0
     

     
     
195872
                           gb|AAB59063.1|
                           (J03153) MinE protein [Escherichia coli]
                           89
                           61.79
                           76.40
                           6
                           253.0
     

     






      

          
GC ORFID: 48313How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3245Glimmer Score: 

          
Start: 3039132Stop: 3039386

          
Length: 255


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3245 translation of PA3245Homolog Bit Score 166.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2671 (ORF: BLASTX 130.0), t_PA3245 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCTTTTAGACTTCTTTCGCAGTCGGAAATCGCAGAACAGCGCTTCCATCGCGAAAGAACGACTGCAG 
ATCATCGTTGCCCACGAACGTGGCCAGCGCGCACAGCCGGATTATCTGCCCCAGTTGCAGAAAGACCTGCTG 
GAAGTGATCCGCAAGTACGTACCGATCGACCAGGAGCAGATCCAGGTGGAACTGGAGAACCAGGGCAACTGC 
TCGATCCTGGAACTCAACATCACCCTGCCGGATCGTTGA