ViewGene
Locus Name:
GeneID:
Location:

GeneID:531
TrivialName: PA14_48700
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_48700
PAO1 Ortholog Locus:PA1207
Sequence Length:1842
Protein Length:613
Start: 4332289
Stop: 4330448
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04596.1|AE004550_12 (AE004550) glutathione-regulated potassium-efflux system protein KefB [Pseudomonas aeruginosa PAO1]
Identities = 612/613 (99%), Positives = 613/613 (100%)
Sequence:
GTGGAACACGGAAGCAGTTTTCTCCAGTCCGGCGTGGTCTTCCTGATCGCGGCGGTGTTCATGGTGCCGCTG
GCCAAGCGCCTGCAACTGGGCGCGGTGCTCGGCTACCTGCTGGCCGGCGTGCTGATCGGTCCCAGCGTGCTG
GGCCTGGTGGACAACCCGGAAAGCGTCGCGCAGATCTCCGAGATGGGCGTGGTATTGCTGCTGTTCATCATC
GGCCTGGAGCTGTCGCCGCGGCGCCTGTGGGTGATGCGCAAGTCGGTGTTCGGCGTCGGCCTGCTGCAGGTG
CTGCTGACCGGCGTGGTGATCGGCACCGTGGCCCTGGTCGGCTTCGACCAACCGGTGAACAGCGCGGTGGTG
CTCGGCCTGGGCCTGGCGCTGTCGTCCACCGCCTTCGGCCTGCAGATCCTCGCCGAGCGCAAGGAACTGACC
CAGCCCCATGGCCGCCTGGCCTTCGCCATCCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGATCGCCATG
ATCCCGCTGCTCAGCGGTGCGCACAGCGACGCCGGCGGTAGCGAACTGACCCAGACCCTCAAGGTAGTCGGC
AGCATCGCCGCGGTGGTGGTCGGTGGCCGCTACCTGCTGCGCCCGGTGTTCCGCATCGTCGCCCAGACCAAG
CTGCAGGACATGTCCACCGCCACTGCGCTGCTGGTGGTGATGGGTACCGCGCTGCTGATGGAGCTGGCCGGC
GTGTCCATGGCCCTCGGTGCCTTCCTCGCCGGCCTGCTGCTGGCCGACTCGGAGTACCGCCACGAACTGGAG
GCGCAGATCGAGCCGTTCAAGGGCCTGTTGCTCGGCCTGTTCTTCATCAGCGTGGGGATGAGCGCGGACATC
AGCCTGCTGCTCAAGTCGCCGTGGATGGTGCTCGGCCTGACTGTGCTGCTGATCGCCCTGAAGGCGCCGGTG
CTGTACTTCGTCGGACGCATTTCCGGCGGCCTCGACAAGGCCTCGGCGCTGCGCCTGGGCGTGGTCCTGGCG
GCCGGCGGCGAGTTCGCCTTCGTCGTGTTCAAGATGGCCCGCGACCAGGGCCTGTTCCAGAACGACCTGCAC
AACCTGCTGGTGCTTTCCATCACCCTGTCGATGGCCCTGACCCCGTTGCTGGTACTCGGCCTCGCGCGCCTG
CTGGCGAAGGAGCCGGAGGCGACGAAGCCGCCGCCGGAAATGGAACGCATCGACAACGACGACACCCCGCGC
GTGGTGATCGCCGGGGTCGGCCGCATGGGCCAGATCGTCGCTCGCGTGCTGCGCGCCCAGCGCGTGCCGTTC
ATCGCCCTGGACACCTCGGTGGAGACCATCGAGCTGACCCGTACCCTCGGCGCGATCCCGATCTTCTACGGC
GACCCGATGCGCCCGGAGATCCTTCGCGCGGCCCAGGTAGAGAAGGCCGAGTTCTTCGTCATCGCCACCGAT
GACCCGGAGACCAACATCGAGACGGCCAAGCGCGTGCGCAAGCTGTATCCGCACCTGAAGATCATCGCCCGC
GCGCGTAACCGCCAGCACGTCCACCTGTTGCTCGACGCCGGGGTCGAGCCGGTGCGCGAGACCTTCTTCTCC
AGCCTGGAGATGAGCCGCATGGTGCTCTGCGGCCTGGGTCTGAGCGAGGAACAGGCGGACGCGCGGATCCGT
CGTTTCCGCCGGCACGACGAAGAGGTCCTGGCCTCCCAGCACCTGTTCTACAACGACCGCGACGCGCTGATC
AAAAGCGCTCGCGAGGCGCGGGTCGAGCTGGAGACGCTGTTCCAGACCGACCAGTTGGAAGACCGTGGCGCG
GTAGCGGCGGAGACCCCGGAGAAACCTTCCCGCGCGTCCTAG
Translation:
MEHGSSFLQSGVVFLIAAVFMVPLAKRLQLGAVLGYLLAGVLIGPSVLGLVDNPESVAQISEMGVVLLLFII
GLELSPRRLWVMRKSVFGVGLLQVLLTGVVIGTVALVGFDQPVNSAVVLGLGLALSSTAFGLQILAERKELT
QPHGRLAFAILLFQDIAAIPLIAMIPLLSGAHSDAGGSELTQTLKVVGSIAAVVVGGRYLLRPVFRIVAQTK
LQDMSTATALLVVMGTALLMELAGVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMSADI
SLLLKSPWMVLGLTVLLIALKAPVLYFVGRISGGLDKASALRLGVVLAAGGEFAFVVFKMARDQGLFQNDLH
NLLVLSITLSMALTPLLVLGLARLLAKEPEATKPPPEMERIDNDDTPRVVIAGVGRMGQIVARVLRAQRVPF
IALDTSVETIELTRTLGAIPIFYGDPMRPEILRAAQVEKAEFFVIATDDPETNIETAKRVRKLYPHLKIIAR
ARNRQHVHLLLDAGVEPVRETFFSSLEMSRMVLCGLGLSEEQADARIRRFRRHDEEVLASQHLFYNDRDALI
KSAREARVELETLFQTDQLEDRGAVAAETPEKPSRAS*
AnnotationID:540GeneID:531
AnnotatorUID: diggins
Modification Date/Time: 2005-02-21 19:07:56
Gene Name:kefB
Confidence Code:2
GeneProduct:glutathione-regulated potassium-efflux system protein KefB
Cell Localization:()
Synonyms:trkB,kefC
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:glutathione-regulated potassium-proton antiporter
Functional Category Confidence Code:5
COGs:COG0475,COG4651
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55866.1| (AE016864) glutathione-regulated potassium-efflux system protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 602

 Score =  816 bits (2108), Expect = 0.0
 Identities = 420/601 (69%), Positives = 510/601 (84%), Gaps = 2/601 (0%)

>gb|AAN70811.1|AE016725_4 (AE016793) glutathione-regulated potassium-proton antiporter
           [Pseudomonas putida KT2440]
          Length = 595

 Score =  778 bits (2008), Expect = 0.0
 Identities = 406/594 (68%), Positives = 488/594 (82%), Gaps = 2/594 (0%)

>gb|AAM43213.1| (AE012520) glutathione-regulated potassium-efflux system protein
           [Xanthomonas campestris pv. campestris str. ATCC 33913]
          Length = 603

 Score =  545 bits (1405), Expect = e-154
 Identities = 299/607 (49%), Positives = 422/607 (69%), Gaps = 9/607 (1%)






 
Structural Features:
COG0475, KefB, Kef-type K+ transport systems, membrane components 
[Inorganic ion transport and metabolism]
CD-Length = 397 residues, 100.0% aligned
Score =  253 bits (648), Expect = 9e-68
Identities = 170/402 (42%), Positives = 238/402 (59%), Gaps = 9/402 (2%)

COG4651, RosB, Kef-type K+ transport system, predicted NAD-binding component 
[Inorganic ion transport and metabolism]
CD-Length = 408 residues, 100.0% aligned
Score =  174 bits (443), Expect = 5e-44
Identities = 132/411 (32%), Positives = 206/411 (50%), Gaps = 14/411 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14155




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18207




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37573




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:531_cdd.html


    		




 
URL:

















    ReferenceID:38044




  
Author/Investigator(s):
        Bakker EP, Booth IR, Dinnbier U, Epstein W, Gajewska A.





  
Title:
        Evidence for multiple K+ export systems in Escherichia coli.




  
PubMed:
        3301813


MedLine:
        




    
Source:
J Bacteriol. 1987 Aug;169(8):3743-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3301813

















    ReferenceID:38045




  
Author/Investigator(s):
        Munro AW, Ritchie GY, Lamb AJ, Douglas RM, Booth IR.





  
Title:
        The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli.




  
PubMed:
        2046548


MedLine:
        




    
Source:
Mol Microbiol. 1991 Mar;5(3):607-16.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2046548









           
Homologs By Global Alignment 

           

       



     
Gene ID:531
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
928
                           PA1207_tr
                           translation of PA1207
                           613
                           99.83
                           99.99
                           0
                           2988.0
     

     
     
37313
                           gb|AAG04596.1|AE004550_12
                           (AE004550) glutathione-regulated potassium-efflux system protein KefB [Pseudomonas aeruginosa PAO1]
                           613
                           99.67
                           99.99
                           0
                           2985.0
     

     
     
37314
                           gb|AAO55866.1|
                           (AE016864) glutathione-regulated potassium-efflux system protein [Pseudomonas syringae pv. tomato str. DC3000]
                           614
                           68.24
                           83.06
                           13
                           2106.0
     

     
     
37315
                           gb|AAN70811.1|AE016725_4
                           (AE016793) glutathione-regulated potassium-proton antiporter [Pseudomonas putida KT2440]
                           614
                           65.96
                           79.47
                           20
                           2003.0
     

     
     
37316
                           gb|AAM38915.1|
                           (AE012056) glutathione-regulated potassium-efflux system protein [Xanthomonas axonopodis pv. citri str. 306]
                           629
                           49.12
                           68.36
                           35
                           1458.0
     

     






      

          
GC ORFID: 43533How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1207Glimmer Score: 

          
Start: 621619Stop: 623460

          
Length: 1842


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA1207 translation of PA1207Homolog Bit Score 1155.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene334 (ORF: BLASTX 809.0), t_PA1207 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGAACACGGAAGCAGTTTTCTCCAGTCCGGCGTGGTCTTCCTGATCGCGGCGGTGTTCATGGTGCCGCTG 
GCCAAGCGCCTGCAACTGGGCGCGGTGCTCGGCTACCTGCTGGCCGGCGTGCTGATCGGTCCCAGCGTGCTG 
GGCCTGGTGGACAACCCGGAAAGCGTCGCGCAGATCTCCGAGATGGGCGTGGTATTGCTGCTGTTCATCATC 
GGCCTGGAGCTGTCGCCGCGGCGCCTGTGGGTGATGCGCAAGTCGGTGTTCGGCGTCGGCCTGCTGCAGGTG 
CTGCTGACCGGCGTGGTGATCGGCACCGTGGCCCTGGTCGGCTTCGACCAACCGGTGAACAGCGCGGTGGTG 
CTCGGCCTGGGCCTGGCGCTGTCGTCCACCGCCTTCGGCCTGCAGATCCTCGCCGAGCGCAAGGAACTGACC 
CAGCCCCATGGCCGCCTGGCCTTCGCCATCCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGATCGCCATG 
ATCCCGCTGCTCAGCGGTGCGCACAGCGACGCCGGCGGTAGCGAACTGACCCAGACCCTCAAGGTAGTCGGC 
AGCATCGCCGCGGTGGTGGTCGGTGGCCGCTACCTGCTGCGCCCGGTGTTCCGCATCGTCGCCCAGACCAAG 
CTGCAGGACATGTCCACCGCCACTGCGCTGCTGGTGGTGATGGGTACCGCGCTGCTGATGGAGCTGGCCGGC 
GTGTCCATGGCCCTCGGTGCCTTCCTCGCCGGCCTGCTGCTGGCCGACTCGGAGTACCGCCACGAACTGGAG 
GCGCAGATCGAGCCGTTCAAGGGCCTGTTGCTCGGCCTGTTCTTCATCAGCGTGGGGATGAGCGCGGACATC 
AGCCTGCTGCTCAAGTCGCCGTGGATGGTGCTCGGCCTGACTGTGCTGCTGATCGCCCTGAAGGCGCCGGTG 
CTGTACTTCGTCGGACGCATTTCCGGCGGCCTCGACAAGGCCTCGGCGCTGCGCCTGGGCGTGGTCCTGGCG 
GCCGGCGGCGAGTTCGCCTTCGTCGTGTTCAAGATGGCCCGCGACCAGGGCCTGTTCCAGAACGACCTGCAC 
AACCTGCTGGTGCTTTCCATCACCCTGTCGATGGCCCTGACCCCGTTGCTGGTACTCGGCCTCGCGCGCCTG 
CTGGCGAAGGAGCCGGAGGCGACGAAGCCGCCGCCGGAAATGGAACGCATCGACAACGACGACACCCCGCGC 
GTGGTGATCGCCGGGGTCGGCCGCATGGGCCAGATCGTCGCTCGCGTGCTGCGCGCCCAGCGCGTGCCGTTC 
ATCGCCCTGGACACCTCGGTGGAGACCATCGAGCTGACCCGTACCCTCGGCGCGATCCCGATCTTCTACGGC 
GACCCGATGCGCCCGGAGATCCTTCGCGCGGCCCAGGTAGAGAAGGCCGAGTTCTTCGTCATCGCCACCGAT 
GACCCGGAGACCAACATCGAGACGGCCAAGCGCGTGCGCAAGCTGTATCCGCACCTGAAGATCATCGCCCGC 
GCGCGTAACCGCCAGCACGTCCACCTGTTGCTCGACGCCGGGGTCGAGCCGGTGCGCGAGACCTTCTTCTCC 
AGCCTGGAGATGAGCCGCATGGTGCTCTGCGGCCTGGGTCTGAGCGAGGAACAGGCGGACGCGCGGATCCGT 
CGTTTCCGCCGGCACGACGAAGAGGTCCTGGCCTCCCAGCACCTGTTCTACAACGACCGCGACGCGCTGATC 
AAAAGCGCTCGCGAGGCGCGGGTCGAGCTGGAGACGCTGTTCCAGACCGACCAGTTGGAAGACCGTGGCGCG 
GTAGCGGCGGAGACCCCGGAGAAACCTTCCCGCGCGTCCTAG