ViewGene
Locus Name:
GeneID:
Location:

GeneID:5286
TrivialName: PA14_60450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60450
PAO1 Ortholog Locus:PA4567
Sequence Length:258
Protein Length:85
Start: 5388754
Stop: 5388497
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07955.1|AE004870_6 (AE004870) 50S ribosomal protein L27 [Pseudomonas aeruginosa PAO1]
Identities = 85/85 (100%), Positives = 85/85 (100%)
Sequence:
ATGGCACACAAAAAAGCTGGCGGTTCTACCCGCAACGGCCGCGACTCAGAAAGTAAACGCCTTGGCGTGAAA
CTGTTCGGCGGCCAGGCTGTAAAGGCTGGCAACATCCTGGTTCGTCAGCGCGGCACCAAATTCCACGCCGGT
TACGGCGTTGGCCTGGGCAAGGACCACACCCTGTTCGCGAAAGTGGACGGCGTGGTCAAGTTTGAGACCAAA
GGCGCCTTTGGTCGCAAATACGTGAGCATCGTTGCTGCCTAA
Translation:
MAHKKAGGSTRNGRDSESKRLGVKLFGGQAVKAGNILVRQRGTKFHAGYGVGLGKDHTLFAKVDGVVKFETK
GAFGRKYVSIVAA*
AnnotationID:5291GeneID:5286
AnnotatorUID: diggins
Modification Date/Time: 2005-05-05 07:07:31
Gene Name:rpmA
Confidence Code:2
GeneProduct:50S ribosomal subunit protein L27
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0211,pfam01016
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66314.1|AE016259_12 (AE016776) ribosomal protein L27 [Pseudomonas putida KT2440]
          Length = 85

 Score =  151 bits (382), Expect = 7e-37
 Identities = 70/85 (82%), Positives = 80/85 (94%)

>gb|AAN82382.1|AE016767_142 (AE016767) 50S ribosomal protein L27 [Escherichia coli CFT073]
          Length = 85

 Score =  132 bits (332), Expect = 4e-31
 Identities = 64/84 (76%), Positives = 72/84 (85%)






 
Structural Features:
COG0211, RpmA, Ribosomal protein L27 
[Translation, ribosomal structure and biogenesis]
CD-Length = 87 residues,  96.6% aligned
Score =  122 bits (307), Expect = 2e-29
Identities = 69/85 (81%), Positives = 72/85 (84%), Gaps = 1/85 (1%)

pfam01016, Ribosomal_L27, Ribosomal L27 protein.
CD-Length = 81 residues, 100.0% aligned
Score =  106 bits (265), Expect = 1e-24
Identities = 59/82 (71%), Positives = 66/82 (80%), Gaps = 1/82 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:10572




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:27718




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31646




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:5286_cdd.html


    		




 
URL:

















    ReferenceID:33109




  
Author/Investigator(s):
        Maguire BA, Manuilov AV, Zimmermann RA.





  
Title:
        Differential effects of replacing Escherichia coli ribosomal protein L27 with its homologue from Aquifex aeolicus.




  
PubMed:
        11673426


MedLine:
        




    
Source:
J Bacteriol. 2001 Nov;183(22):6565-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11673426

















    ReferenceID:33110




  
Author/Investigator(s):
        Simpson DA, Speert DP.





  
Title:
        RpmA is required for nonopsonic phagocytosis of Pseudomonas aeruginosa.




  
PubMed:
        10768936


MedLine:
        




    
Source:
Infect Immun. 2000 May;68(5):2493-502.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10768936

















    ReferenceID:33111




  
Author/Investigator(s):
        Wower IK, Wower J, Zimmermann RA.





  
Title:
        Ribosomal protein L27 participates in both 50 S subunit assembly and the peptidyl transferase reaction.




  
PubMed:
        9677420


MedLine:
        




    
Source:
J Biol Chem. 1998 Jul 31;273(31):19847-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9677420

















    ReferenceID:33112




  
Author/Investigator(s):
        Jeong JH, Kitakawa M, Isono S, Isono K.





  
Title:
        Cloning and nucleotide sequencing of the genes, rpIU and rpmA, for ribosomal proteins L21 and L27 of Escherichia coli.




  
PubMed:
        8312607


MedLine:
        




    
Source:
DNA Seq. 1993;4(1):59-67.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8312607

















    ReferenceID:33113




  
Author/Investigator(s):
        Kitakawa M, Dabbs ER, Isono K.





  
Title:
        Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli.




  
PubMed:
        378941


MedLine:
        




    
Source:
J Bacteriol. 1979 Jun;138(3):832-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=378941









           
Homologs By Global Alignment 

           

       



     
Gene ID:5286
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7689
                           PA4567_tr
                           translation of PA4567
                           85
                           99.99
                           99.99
                           0
                           434.0
     

     
     
195668
                           gb|AAG07955.1|AE004870_6
                           (AE004870) 50S ribosomal protein L27 [Pseudomonas aeruginosa PAO1]
                           85
                           99.99
                           99.99
                           0
                           434.0
     

     
     
195669
                           gb|AAN66314.1|AE016259_12
                           (AE016776) ribosomal protein L27 [Pseudomonas putida KT2440]
                           85
                           82.35
                           94.11
                           0
                           382.0
     

     
     
195671
                           gb|AAD47626.1|
                           (AF153712) 50S ribosomal protein homolog [Pseudomonas sp. BG33R]
                           85
                           80.00
                           94.11
                           0
                           378.0
     

     
     
195677
                           dbj|BAC93235.1|
                           (AP005331) ribosomal protein L27 [Vibrio vulnificus YJ016]
                           85
                           78.82
                           84.70
                           0
                           337.0
     

     






      

          
GC ORFID: 48288How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4567Glimmer Score: 

          
Start: 4652434Stop: 4652177

          
Length: 258


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4567 translation of PA4567Homolog Bit Score 171.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3527 (ORF: BLASTX 150.0), t_PA4567 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCACACAAAAAAGCTGGCGGTTCTACCCGCAACGGCCGCGACTCAGAAAGTAAACGCCTTGGCGTGAAA 
CTGTTCGGCGGCCAGGCTGTAAAGGCTGGCAACATCCTGGTTCGTCAGCGCGGCACCAAATTCCACGCCGGT 
TACGGCGTTGGCCTGGGCAAGGACCACACCCTGTTCGCGAAAGTGGACGGCGTGGTCAAGTTTGAGACCAAA 
GGCGCCTTTGGTCGCAAATACGTGAGCATCGTTGCTGCCTAA