ViewGene
Locus Name:
GeneID:
Location:

GeneID:528
TrivialName: PA14_66750
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66750
PAO1 Ortholog Locus:PA5051
Sequence Length:1764
Protein Length:587
Start: 5961348
Stop: 5963111
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08436.1|AE004918_6 (AE004918) arginyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 586/587 (99%), Positives = 587/587 (100%)
Sequence:
ATGAAAGACACGATTCGCCAGCTGATCCAGCAAGCCCTCGACCAACTGACCGCGGACGGCACCCTGCCCGCC
GGCCTGACCCCGGACATCCAGGTGGAGAACACCAAGGACCGTAGCCACGGCGATTTCGCCAGCAACATCGCC
ATGATGCTGGCCAAGCCGGCCGGGATGAAGCCGCGCGACCTGGCCGCCAGGCTGGTCGAGGCAATCCCCGCC
CACGAGCAACTGGCCAAGGTGGAGATCGCCGGCCCCGGCTTCCTCAACTTCTTCCAGGATCACGTCTGGCTC
GCCGCCAGCCTCGACCGCGCCCTCGCCGACGAACGTCTCGGCGTGCGCAAGGCAGGCCCGGCGCAGCGCGTG
GTGATCGACCTGTCCTCGCCGAACCTGGCCAAGGAGATGCACGTCGGCCACCTGCGCTCGACCATCATCGGC
GACGCCGTCGCGCGCGTCCTCGAGTTCCTCGGCGACACGGTGATCCGGCAGAACCACGTCGGCGACTGGGGC
ACCCAGTTCGGCATGCTGCTCGCCTACCTGGAAGAGCAACCGGTCGACGCCGAGGCCGAGCTGCACGACCTG
GAGGTCTTCTACCGCGCGGCGAAGAAACGCTTCGACGAGTCTCCCGAATTCGCCGACCGCGCCCGCGAACTG
GTGGTCAAGCTGCAGGCCGGCGACCCTGACTGCCTGCGCCTGTGGACCCGCTTCAACGAGATTTCCCTGTCG
CACTGCCAGAAGGTCTACGACCGTCTCGGCGTGAAGCTGAGCATGGCCGACGTCATGGGCGAGAGCGCCTAC
AACGACGACCTCGCCCAGGTGGTCGCCGACCTGACCGCCAAGGGCCTGCTCACCGAAGACAACGGCGCGTTG
TGCGTCTTCCTCGAAGAGTTCAGGAACGCCGAGGGCAATCCACTCCCGGTGATCGTGCAGAAGGCCGGCGGC
GGCTACCTCTACGCCACCACCGACCTCGCCGCCATGCGTTATCGGCACAACGTGCTGCACGCCGACCGCGTG
CTGTACTTCGTCGACCAGCGCCAGGCCCTGCACTTCCAGCAGGTCTTCGAAGTGGCGCGGCGCGCCGGTTTC
GTTCCGGCCGGCATGGAGCTGGAGCACATGGGCTTCGGTACCATGAACGGCGCCGACGGCCGTCCGTTCAAG
ACCCGCGACGGCGGTACGGTGAAGCTGATCGACCTGCTGGAAGAGGCCGAGAGCCGCGCCTATGCGCTGGTC
AAGGAACGCAACGAGCAGCGCGCCGAGCGTGGCGAGGAGCCGTTCGACGAGGTCCAGCTGCGCGAGATCGGC
CGGGTGGTGGGGATCGACTCGGTGAAGTACGCCGACCTGTCCAAGCACCGCACCAGCGACTACAGCTTCAAC
TTCGAACTGATGCTCAGCTTCGAAGGCAACACCGCACCCTACCTGCTCTACGCCTGCACCCGCGTGGCCAGC
GTCTTCCGCAAGCTCGGCCAGGGCCGCGAGCAACTGGGCGGGAAGATCGTCCTCGAGCAGCCCCAGGAGCTG
GCCCTGGCCGCACAACTGGCGCAGTTCGGCGACCTGATCAACAACGTCGCGCTCAAGGGCGTGCCGCACCTG
CTCTGCGCCTACCTGTACGAGTTGGCCGGGCTGTTCTCCAGTTTCTACGAGCACTGCCCGATCCTCACCGCC
GAGGACCCGGCGCAGAAGGACAGCCGCCTGCGTCTCGCCGCGCTGACCGGCCGTACCCTGGAACAGGGCCTG
GAGCTGCTCGGTCTGAAGACTCTGGAACGCATGTAA
Translation:
MKDTIRQLIQQALDQLTADGTLPAGLTPDIQVENTKDRSHGDFASNIAMMLAKPAGMKPRDLAARLVEAIPA
HEQLAKVEIAGPGFLNFFQDHVWLAASLDRALADERLGVRKAGPAQRVVIDLSSPNLAKEMHVGHLRSTIIG
DAVARVLEFLGDTVIRQNHVGDWGTQFGMLLAYLEEQPVDAEAELHDLEVFYRAAKKRFDESPEFADRAREL
VVKLQAGDPDCLRLWTRFNEISLSHCQKVYDRLGVKLSMADVMGESAYNDDLAQVVADLTAKGLLTEDNGAL
CVFLEEFRNAEGNPLPVIVQKAGGGYLYATTDLAAMRYRHNVLHADRVLYFVDQRQALHFQQVFEVARRAGF
VPAGMELEHMGFGTMNGADGRPFKTRDGGTVKLIDLLEEAESRAYALVKERNEQRAERGEEPFDEVQLREIG
RVVGIDSVKYADLSKHRTSDYSFNFELMLSFEGNTAPYLLYACTRVASVFRKLGQGREQLGGKIVLEQPQEL
ALAAQLAQFGDLINNVALKGVPHLLCAYLYELAGLFSSFYEHCPILTAEDPAQKDSRLRLAALTGRTLEQGL
ELLGLKTLERM*
AnnotationID:537GeneID:528
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 11:11:47
Gene Name:argS
Confidence Code:2
GeneProduct:arginyl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0018,cd00810,pfam00750
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:6.1.1.19
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70654.1|AE016709_6 (AE016793) arginyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 578

 Score =  894 bits (2311), Expect = 0.0
 Identities = 455/587 (77%), Positives = 508/587 (86%), Gaps = 9/587 (1%)

>gb|AAO58565.1| (AE016874) arginyl-tRNA synthetase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 578

 Score =  879 bits (2270), Expect = 0.0
 Identities = 451/587 (76%), Positives = 500/587 (85%), Gaps = 9/587 (1%)

>gb|AAN57096.1|AE015843_8 (AE015843) arginyl-tRNA synthetase [Shewanella oneidensis MR-1]
          Length = 581

 Score =  721 bits (1861), Expect = 0.0
 Identities = 379/591 (64%), Positives = 440/591 (74%), Gaps = 14/591 (2%)








 
Structural Features:
COG0018, ArgS, Arginyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]
CD-Length = 577 residues,  99.7% aligned
Score =  611 bits (1576), Expect = 1e-175
Identities = 242/596 (40%), Positives = 338/596 (56%), Gaps = 30/596 (5%)

cd00810, ArgRS_core_K_HIGH, This is the catalytic core domain of 
Arginyl tRNA synthetase (ArgRS).
CD-Length = 266 residues,  99.6% aligned
Score =  345 bits (887), Expect = 7e-96

pfam00750, tRNA-synt_1d, tRNA synthetases class I (R).
CD-Length = 353 residues,  99.7% aligned
Score =  311 bits (798), Expect = 1e-85
Identities = 145/369 (39%), Positives = 201/369 (54%), Gaps = 28/369 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1059




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18201




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35033




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:528_cdd.html


    		




 
URL:

















    ReferenceID:35836




  
Author/Investigator(s):
        Eriani G, Dirheimer G, Gangloff J.





  
Title:
        Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase.




  
PubMed:
        2668891


MedLine:
        




    
Source:
Nucleic Acids Res. 1989 Jul 25;17(14):5725-36.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2668891

















    ReferenceID:35837




  
Author/Investigator(s):
        Oguiza JA, Malumbres M, Eriani G, Pisabarro A, Mateos LM, Martin F, Martin JF.





  
Title:
        A gene encoding arginyl-tRNA synthetase is located in the upstream region of the lysA gene in Brevibacterium lactofermentum: regulation of argS-lysA cluster expression by arginine.




  
PubMed:
        8226683


MedLine:
        




    
Source:
J Bacteriol. 1993 Nov;175(22):7356-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8226683









           
Homologs By Global Alignment 

           

       



     
Gene ID:528
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
925
                           PA5051_tr
                           translation of PA5051
                           587
                           99.82
                           99.99
                           0
                           2997.0
     

     
     
37226
                           gb|AAG08436.1|AE004918_6
                           (AE004918) arginyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           587
                           99.82
                           99.99
                           0
                           2997.0
     

     
     
37227
                           gb|AAN70654.1|AE016709_6
                           (AE016793) arginyl-tRNA synthetase [Pseudomonas putida KT2440]
                           587
                           77.85
                           86.71
                           9
                           2320.0
     

     
     
37228
                           gb|AAO58565.1|
                           (AE016874) arginyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           587
                           76.83
                           85.17
                           9
                           2277.0
     

     
     
37229
                           gb|AAN57096.1|AE015843_8
                           (AE015843) arginyl-tRNA synthetase [Shewanella oneidensis MR-1]
                           596
                           63.92
                           73.99
                           24
                           1877.0
     

     






      

          
GC ORFID: 43530How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5051Glimmer Score: 

          
Start: 5225031Stop: 5226794

          
Length: 1764


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5051 translation of PA5051Homolog Bit Score 1159.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3787 (ORF: BLASTX 877.0), t_PA5051 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAGACACGATTCGCCAGCTGATCCAGCAAGCCCTCGACCAACTGACCGCGGACGGCACCCTGCCCGCC 
GGCCTGACCCCGGACATCCAGGTGGAGAACACCAAGGACCGTAGCCACGGCGATTTCGCCAGCAACATCGCC 
ATGATGCTGGCCAAGCCGGCCGGGATGAAGCCGCGCGACCTGGCCGCCAGGCTGGTCGAGGCAATCCCCGCC 
CACGAGCAACTGGCCAAGGTGGAGATCGCCGGCCCCGGCTTCCTCAACTTCTTCCAGGATCACGTCTGGCTC 
GCCGCCAGCCTCGACCGCGCCCTCGCCGACGAACGTCTCGGCGTGCGCAAGGCAGGCCCGGCGCAGCGCGTG 
GTGATCGACCTGTCCTCGCCGAACCTGGCCAAGGAGATGCACGTCGGCCACCTGCGCTCGACCATCATCGGC 
GACGCCGTCGCGCGCGTCCTCGAGTTCCTCGGCGACACGGTGATCCGGCAGAACCACGTCGGCGACTGGGGC 
ACCCAGTTCGGCATGCTGCTCGCCTACCTGGAAGAGCAACCGGTCGACGCCGAGGCCGAGCTGCACGACCTG 
GAGGTCTTCTACCGCGCGGCGAAGAAACGCTTCGACGAGTCTCCCGAATTCGCCGACCGCGCCCGCGAACTG 
GTGGTCAAGCTGCAGGCCGGCGACCCTGACTGCCTGCGCCTGTGGACCCGCTTCAACGAGATTTCCCTGTCG 
CACTGCCAGAAGGTCTACGACCGTCTCGGCGTGAAGCTGAGCATGGCCGACGTCATGGGCGAGAGCGCCTAC 
AACGACGACCTCGCCCAGGTGGTCGCCGACCTGACCGCCAAGGGCCTGCTCACCGAAGACAACGGCGCGTTG 
TGCGTCTTCCTCGAAGAGTTCAGGAACGCCGAGGGCAATCCACTCCCGGTGATCGTGCAGAAGGCCGGCGGC 
GGCTACCTCTACGCCACCACCGACCTCGCCGCCATGCGTTATCGGCACAACGTGCTGCACGCCGACCGCGTG 
CTGTACTTCGTCGACCAGCGCCAGGCCCTGCACTTCCAGCAGGTCTTCGAAGTGGCGCGGCGCGCCGGTTTC 
GTTCCGGCCGGCATGGAGCTGGAGCACATGGGCTTCGGTACCATGAACGGCGCCGACGGCCGTCCGTTCAAG 
ACCCGCGACGGCGGTACGGTGAAGCTGATCGACCTGCTGGAAGAGGCCGAGAGCCGCGCCTATGCGCTGGTC 
AAGGAACGCAACGAGCAGCGCGCCGAGCGTGGCGAGGAGCCGTTCGACGAGGTCCAGCTGCGCGAGATCGGC 
CGGGTGGTGGGGATCGACTCGGTGAAGTACGCCGACCTGTCCAAGCACCGCACCAGCGACTACAGCTTCAAC 
TTCGAACTGATGCTCAGCTTCGAAGGCAACACCGCACCCTACCTGCTCTACGCCTGCACCCGCGTGGCCAGC 
GTCTTCCGCAAGCTCGGCCAGGGCCGCGAGCAACTGGGCGGGAAGATCGTCCTCGAGCAGCCCCAGGAGCTG 
GCCCTGGCCGCACAACTGGCGCAGTTCGGCGACCTGATCAACAACGTCGCGCTCAAGGGCGTGCCGCACCTG 
CTCTGCGCCTACCTGTACGAGTTGGCCGGGCTGTTCTCCAGTTTCTACGAGCACTGCCCGATCCTCACCGCC 
GAGGACCCGGCGCAGAAGGACAGCCGCCTGCGTCTCGCCGCGCTGACCGGCCGTACCCTGGAACAGGGCCTG 
GAGCTGCTCGGTCTGAAGACTCTGGAACGCATGTAA