ViewGene
Locus Name:
GeneID:
Location:

GeneID:527
TrivialName: PA14_62160
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62160
PAO1 Ortholog Locus:PA4696
Sequence Length:1725
Protein Length:574
Start: 5547664
Stop: 5545940
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08082.1|AE004883_6 (AE004883) acetolactate synthase large subunit [Pseudomonas aeruginosa PAO1]
Identities = 574/574 (100%), Positives = 574/574 (100%)
Sequence:
GTGGAGCTTTTATCAGGCGCTGAAATGGTCGTCCGCTCGTTGCGCGACGAAGGCGTTAAGTACATCTACGGG
TACCCGGGTGGTGCCCTCCTGCATATCTACGACGCGCTCTTCAAGGAGCACGACGTGACCCACATCCTGGTG
CGCCACGAACAGGCGGCTACCCATATGGCCGACGGCTACGCCCGCGCCACCGGCAAGCCCGGTGTCGTGCTG
GTGACTTCCGGCCCGGGCGCGACCAACGCCATCACCGGTATCGCCACCGCATACATGGACTCCATCCCGATG
GTGATCCTGTCGGGCCAGGTGCCGAGCAACATGGTCGGTACCGACGCGTTCCAGGAAACCGACATGGTCGGG
ATCTCCCGGCCGATCGTGAAGCACAGCTTCATCATCAAGCATCCCTCGGAAATTCCCGAGGTGATCAAGAAG
GCCTTTTACCTGGCGCAGTCGGGTCGTCCGGGGCCGGTGGTCGTGGATATTCCGAAGGACATGGGCGACCCG
ACGCAGAAGTTCGAATATTCCTATCCGAAGAAGGTCAAGTTGCGTTCGTACAGCCCGGCCGTTCGCGGTCAC
TCGGGACAGATCCGCAAGGCCGCCGAGATGCTCCTGGCCGCCAAGCGCCCGGTGGTCTATTCCGGCGGCGGC
GTGATCATGGGCAATGCCGCGGCGCCGCTGACCGAGCTGGCGCAGATGCTCAACCTGCCGGTGACCAACACC
CTGATGGGCCTCGGCGGGTATCCCGGCGCGGACCGCCAGTTCCTCGGCATGCTCGGGATGCACGGCAGCTTC
ACCGCCAACCTGGCGATGCATCACAGCGACGTGATCCTCGCGGTCGGCGCGCGTTTCGACGACCGGGTCATC
AACGGCGCGGCGAAGTTCTGCCCGAACGCCAAGATCATCCACATCGACATCGATCCGGCGTCGATTTCCAAG
ACCATCAAGGCCGACATCCCGATCGTCGGCCCGGTGGACAGCGTTCTCACCGAAATGGTCGCGATCGTCAGG
GAAATCGGCGAGACCCCGAACCAGGATGCCCAGGCTGCCTGGTGGAAGCAGATCGACGAGTGGCGTGGTAAC
CGTGGGTTGTTCCCGTACGACAAGGGCGACGGCAGCATCATCAAGCCGCAGACCGTCATCGAGACGCTCTAC
GAGGTTACCCATGGCGATGCCTTCATCACCTCCGATGTCGGCCAGCACCAGATGTTCGCCTCGCAGTACTAC
AAGTTCAACAAGCCCAATCGCTGGATCAACTCCGGCGGTCTCGGCACCATGGGCTTCGGCTTCCCGGCGGCG
ATGGGCATCAAGCTCAACTTCCCGGACGACGATGTCGCCTGCGTGACCGGCGAGGGCAGTATCCAGATGAAT
ATCCAGGAACTGTCTACCTGCCTGCAGTACGACCTGCCGGTGAAGATCGTCAACCTGAACAATGGTGCCCTG
GGCATGGTCCGCCAGTGGCAGGACATGCAGTACAACAGCCGTTATTCGCATTCCTACATGGAATCCCTGCCG
GACTTCGTCAAGCTTGCCGAAGCCTACGGGCATGTCGGCATGCGCATCACCGACCTGAAGGATCTGAAGCCG
AAGATGGAGGAGGCGTTCGCCATGAAGAATCGCCTGGTCTTCCTCGACATCCAGGTGGACGCCAGCGAACAC
GTCTATCCGATGCAGATCCGTGACGGCGCGATGCGCGACATGTGGCTGAGCAAGACGGAGCGTACCTGA
Translation:
MELLSGAEMVVRSLRDEGVKYIYGYPGGALLHIYDALFKEHDVTHILVRHEQAATHMADGYARATGKPGVVL
VTSGPGATNAITGIATAYMDSIPMVILSGQVPSNMVGTDAFQETDMVGISRPIVKHSFIIKHPSEIPEVIKK
AFYLAQSGRPGPVVVDIPKDMGDPTQKFEYSYPKKVKLRSYSPAVRGHSGQIRKAAEMLLAAKRPVVYSGGG
VIMGNAAAPLTELAQMLNLPVTNTLMGLGGYPGADRQFLGMLGMHGSFTANLAMHHSDVILAVGARFDDRVI
NGAAKFCPNAKIIHIDIDPASISKTIKADIPIVGPVDSVLTEMVAIVREIGETPNQDAQAAWWKQIDEWRGN
RGLFPYDKGDGSIIKPQTVIETLYEVTHGDAFITSDVGQHQMFASQYYKFNKPNRWINSGGLGTMGFGFPAA
MGIKLNFPDDDVACVTGEGSIQMNIQELSTCLQYDLPVKIVNLNNGALGMVRQWQDMQYNSRYSHSYMESLP
DFVKLAEAYGHVGMRITDLKDLKPKMEEAFAMKNRLVFLDIQVDASEHVYPMQIRDGAMRDMWLSKTERT*
AnnotationID:536GeneID:527
AnnotatorUID: diggins
Modification Date/Time: 2005-05-24 14:02:10
Gene Name:ilvI
Confidence Code:2
GeneProduct:acetolactate synthase large subunit
Cell Localization:()
Synonyms:ilvB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0028,COG3960,pfam02776
Secondary Functional Category(ies):biosynthesis of cofactors, prosthetic groups and carriers
EC Number:2.2.1.6
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70253.1|AE016665_6 (AE016791) acetolactate synthase, large subunit, biosynthetic type
           [Pseudomonas putida KT2440]
          Length = 574

 Score = 1028 bits (2657), Expect = 0.0
 Identities = 497/574 (86%), Positives = 540/574 (94%)

>gb|AAO54515.1| (AE016859) acetolactate synthase, large subunit, biosynthetic type
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 574

 Score = 1025 bits (2649), Expect = 0.0
 Identities = 494/574 (86%), Positives = 542/574 (94%)

>gb|AAN78592.1|AE016755_92 (AE016755) Acetolactate synthase isozyme III large subunit
           [Escherichia coli CFT073]
          Length = 604

 Score =  756 bits (1951), Expect = 0.0
 Identities = 367/576 (63%), Positives = 452/576 (78%), Gaps = 4/576 (0%)








 
Structural Features:
COG0028, IlvB, Thiamine pyrophosphate-requiring enzymes 
[acetolactate synthase, pyruvate dehydrogenase (cytochrome), 
glyoxylate carboligase, phosphonopyruvate decarboxylase] 
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 550 residues,  99.6% aligned
Score =  675 bits (1742), Expect = 0.0
Identities = 280/563 (49%), Positives = 368/563 (65%), Gaps = 15/563 (2%)

COG3960, COG3960, Glyoxylate carboligase
CD-Length = 592 residues,  91.4% aligned
Score =  318 bits (815), Expect = 2e-87
Identities = 183/547 (33%), Positives = 286/547 (52%), Gaps = 17/547 (3%)

pfam02776, TPP_enzyme_N, Thiamine pyrophosphate enzyme, 
N-terminal TPP binding domain.
CD-Length = 171 residues,  99.4% aligned
Score =  233 bits (595), Expect = 6e-62
Identities = 91/170 (53%), Positives = 122/170 (71%)






   
Genomic Context:






   
Comment:
EC Number formerly 4.1.3.18.


















    ReferenceID:14153




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18199




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37607




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:527_cdd.html


    		




 
URL:

















    ReferenceID:37751




  
Author/Investigator(s):
        Squires CH, De Felice M, Devereux J, Calvo JM.





  
Title:
        Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12.




  
PubMed:
        6308579


MedLine:
        




    
Source:
Nucleic Acids Res. 1983 Aug 11;11(15):5299-313.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6308579

















    ReferenceID:37756




  
Author/Investigator(s):
        Thao ML, Baumann P.





  
Title:
        Sequence analysis of a DNA fragment from buchnera aphidicola (Aphid endosymbiont) containing the genes dapD-htrA-ilvI-ilvH-ftsL-ftsI-murE




  
PubMed:
        0009688822


MedLine:
        




    
Source:
Curr Microbiol. 1998 Sep;37(3):214-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9688822









           
Homologs By Global Alignment 

           

       



     
Gene ID:527
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
924
                           PA4696_tr
                           translation of PA4696
                           574
                           99.99
                           99.99
                           0
                           2997.0
     

     
     
37055
                           gb|AAG08082.1|AE004883_6
                           (AE004883) acetolactate synthase large subunit [Pseudomonas aeruginosa PAO1]
                           574
                           99.82
                           99.99
                           0
                           2994.0
     

     
     
37056
                           gb|AAN70253.1|AE016665_6
                           (AE016791) acetolactate synthase, large subunit, biosynthetic type [Pseudomonas putida KT2440]
                           574
                           86.41
                           94.07
                           0
                           2653.0
     

     
     
37057
                           gb|AAO54515.1|
                           (AE016859) acetolactate synthase, large subunit, biosynthetic type [Pseudomonas syringae pv. tomato str. DC3000]
                           574
                           85.88
                           94.42
                           0
                           2645.0
     

     
     
37058
                           gb|AAN55319.1|AE015669_7
                           (AE015669) acetolactate synthase III, large subunit [Shewanella oneidensis MR-1]
                           574
                           65.15
                           80.31
                           2
                           2024.0
     

     






      

          
GC ORFID: 43529How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4696Glimmer Score: 

          
Start: 4811344Stop: 4809620

          
Length: 1725


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4696 translation of PA4696Homolog Bit Score 1159.0

          
Other Homologs:  psyr_15may02_Scaffold19_revised_gene2873 (ORF: BLASTX 1020.0), t_PA4696 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGAGCTTTTATCAGGCGCTGAAATGGTCGTCCGCTCGTTGCGCGACGAAGGCGTTAAGTACATCTACGGG 
TACCCGGGTGGTGCCCTCCTGCATATCTACGACGCGCTCTTCAAGGAGCACGACGTGACCCACATCCTGGTG 
CGCCACGAACAGGCGGCTACCCATATGGCCGACGGCTACGCCCGCGCCACCGGCAAGCCCGGTGTCGTGCTG 
GTGACTTCCGGCCCGGGCGCGACCAACGCCATCACCGGTATCGCCACCGCATACATGGACTCCATCCCGATG 
GTGATCCTGTCGGGCCAGGTGCCGAGCAACATGGTCGGTACCGACGCGTTCCAGGAAACCGACATGGTCGGG 
ATCTCCCGGCCGATCGTGAAGCACAGCTTCATCATCAAGCATCCCTCGGAAATTCCCGAGGTGATCAAGAAG 
GCCTTTTACCTGGCGCAGTCGGGTCGTCCGGGGCCGGTGGTCGTGGATATTCCGAAGGACATGGGCGACCCG 
ACGCAGAAGTTCGAATATTCCTATCCGAAGAAGGTCAAGTTGCGTTCGTACAGCCCGGCCGTTCGCGGTCAC 
TCGGGACAGATCCGCAAGGCCGCCGAGATGCTCCTGGCCGCCAAGCGCCCGGTGGTCTATTCCGGCGGCGGC 
GTGATCATGGGCAATGCCGCGGCGCCGCTGACCGAGCTGGCGCAGATGCTCAACCTGCCGGTGACCAACACC 
CTGATGGGCCTCGGCGGGTATCCCGGCGCGGACCGCCAGTTCCTCGGCATGCTCGGGATGCACGGCAGCTTC 
ACCGCCAACCTGGCGATGCATCACAGCGACGTGATCCTCGCGGTCGGCGCGCGTTTCGACGACCGGGTCATC 
AACGGCGCGGCGAAGTTCTGCCCGAACGCCAAGATCATCCACATCGACATCGATCCGGCGTCGATTTCCAAG 
ACCATCAAGGCCGACATCCCGATCGTCGGCCCGGTGGACAGCGTTCTCACCGAAATGGTCGCGATCGTCAGG 
GAAATCGGCGAGACCCCGAACCAGGATGCCCAGGCTGCCTGGTGGAAGCAGATCGACGAGTGGCGTGGTAAC 
CGTGGGTTGTTCCCGTACGACAAGGGCGACGGCAGCATCATCAAGCCGCAGACCGTCATCGAGACGCTCTAC 
GAGGTTACCCATGGCGATGCCTTCATCACCTCCGATGTCGGCCAGCACCAGATGTTCGCCTCGCAGTACTAC 
AAGTTCAACAAGCCCAATCGCTGGATCAACTCCGGCGGTCTCGGCACCATGGGCTTCGGCTTCCCGGCGGCG 
ATGGGCATCAAGCTCAACTTCCCGGACGACGATGTCGCCTGCGTGACCGGCGAGGGCAGTATCCAGATGAAT 
ATCCAGGAACTGTCTACCTGCCTGCAGTACGACCTGCCGGTGAAGATCGTCAACCTGAACAATGGTGCCCTG 
GGCATGGTCCGCCAGTGGCAGGACATGCAGTACAACAGCCGTTATTCGCATTCCTACATGGAATCCCTGCCG 
GACTTCGTCAAGCTTGCCGAAGCCTACGGGCATGTCGGCATGCGCATCACCGACCTGAAGGATCTGAAGCCG 
AAGATGGAGGAGGCGTTCGCCATGAAGAATCGCCTGGTCTTCCTCGACATCCAGGTGGACGCCAGCGAACAC 
GTCTATCCGATGCAGATCCGTGACGGCGCGATGCGCGACATGTGGCTGAGCAAGACGGAGCGTACCTGA