ViewGene
Locus Name:
GeneID:
Location:

GeneID:522
TrivialName: PA14_66080
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66080
PAO1 Ortholog Locus:PA4997
Sequence Length:1812
Protein Length:603
Start: 5886941
Stop: 5885130
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08382.1|AE004912_8 (AE004912) transport protein MsbA [Pseudomonas aeruginosa PAO1]
Identities = 599/603 (99%), Positives = 602/603 (99%)
Sequence:
ATGAGCGATAGTCCTCAGAATCCCGGACCTTCCAGTCTCAAGATCTATTTCCGCCTGTTGGGCTACGTGAAG
CCCTACATCGGAATGTTCCTACTCAGCATCGTCGGTTTCCTGATCTTCGCGTCGACCCAGCCGATGCTGGCG
GGCATCCTCAAGTATTTCGTCGACGGCCTCTCCAATCCTGACGCGGCATTGTTTCCGAATGTGCAGTGGCCC
TGGTTGCGCGACCTGCACCTGGTCTACGCGGTGCCGTTGCTGATCATCCTGATCGCTGCCTGGCAGGGGCTG
GGCTCGTTCCTCGGCAACTTCTTCCTGGCCAAGGTTTCCCTGGGGCTGGTGCATGACCTGCGGGTCGCCCTG
TTCAACAAGCTGCTGGTGCTGCCGAACCGCTATTTCGACACGCACAGCTCCGGCCACCTGATCTCGCGCATC
ACCTTCAACGTGACCATGGTCACCGGAGCAGCCACGGATGCCATCAAGGTGGTGATCCGTGAGGGCCTGACC
GTGGTCTTCCTGTTCCTCTACCTGCTGTGGATGAACTGGAAGCTGACCCTGGTGATGCTGGCGATCCTGCCG
GTCATCGCGGTGATGGTGACTACTGCCAGCCGCAAGTTCCGCAAGCAGAGCAAGAAGATCCAGGTGGCCATG
GGCGACGTCACCCACGTCGCCTCGGAAACTATCCAGGGCTACCGGGTGGTGCGCAGCTTCGGCGGCGAAGCC
TATGAAGAGAAGCGCTTCCACGACGCCAGCCAGAGCAATACCGACAAGCAGTTGCGGATGACCAAGACCGGC
GCGGTCTACACGCCGATGCTGCAATTGGTGATCTACGTCGCCATGGCGATACTGATGTTCCTCGTGCTGTGG
CTGCGCGGGGACGCCTCGGCCGGCGACCTGGTGGCCTATATCACCGCGGCGGGCCTTTTGCCCAAACCGATC
CGCCAGCTTTCCGAGGTCAGTTCCACGGTCCAGCGCGGCGTCGCCGGCGCCGAGAGCATCTTCGAGCAGCTC
GACGAAGCGGCGGAGGAAGACCAGGGGAGCGTCGAGAAGGAGCGGGTCAGCGGGCGCCTGGAAGTGCGCAAC
CTGAGCTTCCGTTATCCCGGAACCGACAAGCAGGTGCTGGATGACATCAGTTTCGTCGCCGAACCCGGGCAG
ATGATCGCCCTGGTCGGCCGTTCCGGTAGCGGCAAGTCGACCCTGGCCAACCTGGTTCCGCGCTTTTATCAG
CACAATGACGGCAAGATCATGCTTGATGGCGTTGAGGTCGAGGATTATCGCCTGCGTAACCTGCGCCGCCAC
ATCGCCCTGGTGACCCAGCAGGTCACGCTGTTCAACGACAGCGTGGCCAACAACATCGCCTACGGCGACCTG
GCTGGCGCGCCGCGCGAGGAAATCGAGCGGGCGGCGAAGGCTGCCAACGCCAAGGAATTCATCGACAACCTG
CCGCAAGGCTTCGACACCGAGGTCGGCGAGAATGGCGTGCTGCTTTCCGGCGGCCAGCGCCAGCGTCTGGCG
ATCGCCCGGGCGCTGCTCAAGGACGCGCCCCTGCTGATCCTCGACGAGGCGACCTCGGCGCTGGACACCGAG
TCGGAGCGGCATATTCAGGCGGCGCTGGACGAAGTGATGAAAGGGCGCACCACCCTGGTGATCGCCCACCGC
CTGTCGACCATCGAGAAGGCCGACCTGATCCTGGTGATGGACCAGGGGCAGATCGTCGAGCGCGGCAGCCAC
GCCGAGCTGCTGGCGCAGAACGGCCACTATGCGCGGCTGCACGCGATGGGACTGGATGAGCAGGCGCCGGCG
CCTGTCGGCTGA
Translation:
MSDSPQNPGPSSLKIYFRLLGYVKPYIGMFLLSIVGFLIFASTQPMLAGILKYFVDGLSNPDAALFPNVQWP
WLRDLHLVYAVPLLIILIAAWQGLGSFLGNFFLAKVSLGLVHDLRVALFNKLLVLPNRYFDTHSSGHLISRI
TFNVTMVTGAATDAIKVVIREGLTVVFLFLYLLWMNWKLTLVMLAILPVIAVMVTTASRKFRKQSKKIQVAM
GDVTHVASETIQGYRVVRSFGGEAYEEKRFHDASQSNTDKQLRMTKTGAVYTPMLQLVIYVAMAILMFLVLW
LRGDASAGDLVAYITAAGLLPKPIRQLSEVSSTVQRGVAGAESIFEQLDEAAEEDQGSVEKERVSGRLEVRN
LSFRYPGTDKQVLDDISFVAEPGQMIALVGRSGSGKSTLANLVPRFYQHNDGKIMLDGVEVEDYRLRNLRRH
IALVTQQVTLFNDSVANNIAYGDLAGAPREEIERAAKAANAKEFIDNLPQGFDTEVGENGVLLSGGQRQRLA
IARALLKDAPLLILDEATSALDTESERHIQAALDEVMKGRTTLVIAHRLSTIEKADLILVMDQGQIVERGSH
AELLAQNGHYARLHAMGLDEQAPAPVG*
AnnotationID:531GeneID:522
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 09:09:08
Gene Name:msbA
Confidence Code:2
GeneProduct:transport protein MsbA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:lipid A ABC transporter, ATP-binding/permease protein
Functional Category Confidence Code:5
COGs:COG1132,COG5265,COG4988,COG4987
Secondary Functional Category(ies):defense mechanism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70502.1|AE016692_1 (AE016792) lipid/phospholipid ABC efflux transporter, permease and
           ATP-binding protein, putative [Pseudomonas putida
           KT2440]
          Length = 602

 Score =  992 bits (2564), Expect = 0.0
 Identities = 510/592 (86%), Positives = 552/592 (93%), Gaps = 2/592 (0%)

>gb|AAO58412.1| (AE016874) lipid A ABC transporter, ATP-binding/permease protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 624

 Score =  977 bits (2526), Expect = 0.0
 Identities = 503/597 (84%), Positives = 551/597 (92%), Gaps = 2/597 (0%)

>gb|AAO90389.1| (AE016962) ABC transporter, ATP-binding/permease protein [Coxiella
           burnetii RSA 493]
          Length = 589

 Score =  499 bits (1286), Expect = e-140
 Identities = 254/588 (43%), Positives = 390/588 (66%), Gaps = 13/588 (2%)








 
Structural Features:
COG1132, MdlB, ABC-type multidrug transport system, ATPase and permease components 
[Defense mechanisms]
CD-Length = 567 residues, 100.0% aligned
Score =  478 bits (1230), Expect = 1e-135
Identities = 249/586 (42%), Positives = 360/586 (61%), Gaps = 21/586 (3%)

COG5265, ATM1, ABC-type transport system involved in Fe-S cluster assembly, 
permease and ATPase components
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 497 residues,  99.4% aligned
Score =  301 bits (771), Expect = 2e-82
Identities = 179/499 (35%), Positives = 260/499 (52%), Gaps = 14/499 (2%)

COG4988, CydD, ABC-type transport system involved in cytochrome bd biosynthesis, 
ATPase and permease components 
[Energy production and conversion / 
Posttranslational modification, protein turnover, chaperones]
CD-Length = 559 residues,  93.0% aligned
Score =  287 bits (735), Expect = 3e-78
Identities = 158/525 (30%), Positives = 252/525 (48%), Gaps = 15/525 (2%)

COG4987, CydC, ABC-type transport system involved in cytochrome bd biosynthesis, 
fused ATPase and permease components 
[Energy production and conversion / 
Posttranslational modification, protein turnover, chaperones]
CD-Length = 573 residues,  99.0% aligned
Score =  277 bits (709), Expect = 3e-75
Identities = 164/582 (28%), Positives = 278/582 (47%), Gaps = 25/582 (4%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1047




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18189




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35085




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:522_cdd.html


    		




 
URL:

















    ReferenceID:35874




  
Author/Investigator(s):
        Karow M, Georgopoulos C.





  
Title:
        The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators.




  
PubMed:
        8094880


MedLine:
        




    
Source:
Mol Microbiol. 1993 Jan;7(1):69-79.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8094880









           
Homologs By Global Alignment 

           

       



     
Gene ID:522
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
917
                           PA4997_tr
                           translation of PA4997
                           603
                           99.33
                           99.83
                           0
                           3009.0
     

     
     
36978
                           gb|AAG08382.1|AE004912_8
                           (AE004912) transport protein MsbA [Pseudomonas aeruginosa PAO1]
                           603
                           99.33
                           99.83
                           0
                           3009.0
     

     
     
36979
                           gb|AAN70502.1|AE016692_1
                           (AE016792) lipid/phospholipid ABC efflux transporter, permease and ATP-binding protein, putative [Pseudomonas putida KT2440]
                           605
                           84.79
                           92.39
                           5
                           2567.0
     

     
     
36980
                           gb|AAO58412.1|
                           (AE016874) lipid A ABC transporter, ATP-binding/permease protein [Pseudomonas syringae pv. tomato str. DC3000]
                           628
                           80.25
                           88.05
                           29
                           2528.0
     

     
     
36984
                           gb|AAQ58500.1|
                           (AE016912) transport ATP-binding protein msbA [Chromobacterium violaceum ATCC 12472]
                           608
                           45.88
                           62.50
                           29
                           1244.0
     

     






      

          
GC ORFID: 43524How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4997Glimmer Score: 

          
Start: 5150624Stop: 5148813

          
Length: 1812


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4997 translation of PA4997Homolog Bit Score 1163.0

          
Other Homologs:  t_PA4997 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGATAGTCCTCAGAATCCCGGACCTTCCAGTCTCAAGATCTATTTCCGCCTGTTGGGCTACGTGAAG 
CCCTACATCGGAATGTTCCTACTCAGCATCGTCGGTTTCCTGATCTTCGCGTCGACCCAGCCGATGCTGGCG 
GGCATCCTCAAGTATTTCGTCGACGGCCTCTCCAATCCTGACGCGGCATTGTTTCCGAATGTGCAGTGGCCC 
TGGTTGCGCGACCTGCACCTGGTCTACGCGGTGCCGTTGCTGATCATCCTGATCGCTGCCTGGCAGGGGCTG 
GGCTCGTTCCTCGGCAACTTCTTCCTGGCCAAGGTTTCCCTGGGGCTGGTGCATGACCTGCGGGTCGCCCTG 
TTCAACAAGCTGCTGGTGCTGCCGAACCGCTATTTCGACACGCACAGCTCCGGCCACCTGATCTCGCGCATC 
ACCTTCAACGTGACCATGGTCACCGGAGCAGCCACGGATGCCATCAAGGTGGTGATCCGTGAGGGCCTGACC 
GTGGTCTTCCTGTTCCTCTACCTGCTGTGGATGAACTGGAAGCTGACCCTGGTGATGCTGGCGATCCTGCCG 
GTCATCGCGGTGATGGTGACTACTGCCAGCCGCAAGTTCCGCAAGCAGAGCAAGAAGATCCAGGTGGCCATG 
GGCGACGTCACCCACGTCGCCTCGGAAACTATCCAGGGCTACCGGGTGGTGCGCAGCTTCGGCGGCGAAGCC 
TATGAAGAGAAGCGCTTCCACGACGCCAGCCAGAGCAATACCGACAAGCAGTTGCGGATGACCAAGACCGGC 
GCGGTCTACACGCCGATGCTGCAATTGGTGATCTACGTCGCCATGGCGATACTGATGTTCCTCGTGCTGTGG 
CTGCGCGGGGACGCCTCGGCCGGCGACCTGGTGGCCTATATCACCGCGGCGGGCCTTTTGCCCAAACCGATC 
CGCCAGCTTTCCGAGGTCAGTTCCACGGTCCAGCGCGGCGTCGCCGGCGCCGAGAGCATCTTCGAGCAGCTC 
GACGAAGCGGCGGAGGAAGACCAGGGGAGCGTCGAGAAGGAGCGGGTCAGCGGGCGCCTGGAAGTGCGCAAC 
CTGAGCTTCCGTTATCCCGGAACCGACAAGCAGGTGCTGGATGACATCAGTTTCGTCGCCGAACCCGGGCAG 
ATGATCGCCCTGGTCGGCCGTTCCGGTAGCGGCAAGTCGACCCTGGCCAACCTGGTTCCGCGCTTTTATCAG 
CACAATGACGGCAAGATCATGCTTGATGGCGTTGAGGTCGAGGATTATCGCCTGCGTAACCTGCGCCGCCAC 
ATCGCCCTGGTGACCCAGCAGGTCACGCTGTTCAACGACAGCGTGGCCAACAACATCGCCTACGGCGACCTG 
GCTGGCGCGCCGCGCGAGGAAATCGAGCGGGCGGCGAAGGCTGCCAACGCCAAGGAATTCATCGACAACCTG 
CCGCAAGGCTTCGACACCGAGGTCGGCGAGAATGGCGTGCTGCTTTCCGGCGGCCAGCGCCAGCGTCTGGCG 
ATCGCCCGGGCGCTGCTCAAGGACGCGCCCCTGCTGATCCTCGACGAGGCGACCTCGGCGCTGGACACCGAG 
TCGGAGCGGCATATTCAGGCGGCGCTGGACGAAGTGATGAAAGGGCGCACCACCCTGGTGATCGCCCACCGC 
CTGTCGACCATCGAGAAGGCCGACCTGATCCTGGTGATGGACCAGGGGCAGATCGTCGAGCGCGGCAGCCAC 
GCCGAGCTGCTGGCGCAGAACGGCCACTATGCGCGGCTGCACGCGATGGGACTGGATGAGCAGGCGCCGGCG 
CCTGTCGGCTGA