ViewGene
Locus Name:
GeneID:
Location:

GeneID:5207
TrivialName: PA14_58170
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58170
PAO1 Ortholog Locus:PA4482
Sequence Length:288
Protein Length:95
Start: 5177262
Stop: 5177549
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07870.1|AE004862_4 (AE004862) Glu-tRNA(Gln) amidotransferase subunit C [Pseudomonas aeruginosa PAO1]
Identities = 95/95 (100%), Positives = 95/95 (100%)
Sequence:
ATGGCGCTTGAACGCTCCGACGTGGAAAAAATCGCCCATCTCGCCCGCCTGGGCCTGAGCGAGGCCGATCTT
CCGCGCACCACCGAGACCCTGAACAACATCCTCGGCCTGATCGACCAGATGCAGGCGGTCGACACCAGCGGC
GTCGAACCGCTTGCCCACCCGCTGGAAGCCACCCAGCGCCTGCGTCCGGACGCGGTCACCGAGACCGATCAC
CGCGACGCCTACCAGACCATCGCCCCCGCCGTGGAAGAAGGTCTGTACCTGGTTCCGAAAGTCATCGAGTAA
Translation:
MALERSDVEKIAHLARLGLSEADLPRTTETLNNILGLIDQMQAVDTSGVEPLAHPLEATQRLRPDAVTETDH
RDAYQTIAPAVEEGLYLVPKVIE*
AnnotationID:5212GeneID:5207
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 14:02:32
Gene Name:gatC
Confidence Code:2
GeneProduct:Glu-tRNA(Gln) amidotransferase subunit C
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0721,pfam02686
Secondary Functional Category(ies):
EC Number:6.3.5.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57922.1| (AE016872) glutamyl-tRNA(Gln) amidotransferase, C subunit
          [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 95

 Score =  162 bits (409), Expect = 5e-40
 Identities = 79/95 (83%), Positives = 89/95 (93%)

>gb|AAN66557.1|AE016284_5 (AE016777) glutamyl-tRNA(Gln) amidotransferase, C subunit
           [Pseudomonas putida KT2440]
          Length = 102

 Score =  154 bits (388), Expect = 1e-37
 Identities = 74/95 (77%), Positives = 86/95 (90%)

>emb|CAB84794.1| (AL162756) putative Glu-tRNA(Gln) amidotransferase subunit C
          [Neisseria meningitidis Z2491]
          Length = 96

 Score =  101 bits (252), Expect = 8e-22
 Identities = 49/95 (51%), Positives = 68/95 (71%)






 
Structural Features:
COG0721, GatC, Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit 
[Translation, ribosomal structure and biogenesis]
CD-Length = 96 residues,  99.0% aligned
Score = 98.0 bits (244), Expect = 3e-22
Identities = 45/95 (47%), Positives = 61/95 (64%)

pfam02686, Glu-tRNAGln, Glu-tRNAGln amidotransferase C subunit.
CD-Length = 72 residues, 100.0% aligned
Score = 53.3 bits (128), Expect = 9e-09
Identities = 24/72 (33%), Positives = 40/72 (55%)






   
Genomic Context:






   
Comment:


















    ReferenceID:10414




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:27559




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31524




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:5207_cdd.html


    		




 
URL:

















    ReferenceID:32882




  
Author/Investigator(s):
        Raczniak G, Becker HD, Min B, Soll D.





  
Title:
        A single amidotransferase forms asparaginyl-tRNA and glutaminyl-tRNA in Chlamydia trachomatis.




  
PubMed:
        11585842


MedLine:
        




    
Source:
J Biol Chem. 2001 Dec 7;276(49):45862-7. Epub 2001 Oct 03.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11585842

















    ReferenceID:32883




  
Author/Investigator(s):
        Salazar JC, Zuniga R, Raczniak G, Becker H, Soll D, Orellana O.





  
Title:
        A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans.




  
PubMed:
        11445070


MedLine:
        




    
Source:
FEBS Lett. 2001 Jul 6;500(3):129-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11445070

















    ReferenceID:32884




  
Author/Investigator(s):
        Curnow AW, Hong K, Yuan R, Kim S, Martins O, Winkler W, Henkin TM, Soll D.





  
Title:
        Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.




  
PubMed:
        9342321


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11819-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9342321

















    ReferenceID:32885




  
Author/Investigator(s):
        Nobelmann B, Lengeler JW.





  
Title:
        Molecular analysis of the gat genes from Escherichia coli and of their roles in galactitol transport and metabolism.




  
PubMed:
        8955298


MedLine:
        




    
Source:
J Bacteriol. 1996 Dec;178(23):6790-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8955298









           
Homologs By Global Alignment 

           

       



     
Gene ID:5207
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7617
                           PA4482_tr
                           translation of PA4482
                           96
                           98.95
                           98.95
                           1
                           474.0
     

     
     
195069
                           gb|AAG07870.1|AE004862_4
                           (AE004862) Glu-tRNA(Gln) amidotransferase subunit C [Pseudomonas aeruginosa PAO1]
                           96
                           98.95
                           98.95
                           1
                           474.0
     

     
     
195070
                           gb|AAO57922.1|
                           (AE016872) glutamyl-tRNA(Gln) amidotransferase, C subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           95
                           83.15
                           93.68
                           0
                           409.0
     

     
     
195071
                           gb|AAN66557.1|AE016284_5
                           (AE016777) glutamyl-tRNA(Gln) amidotransferase, C subunit [Pseudomonas putida KT2440]
                           102
                           72.54
                           84.31
                           7
                           388.0
     

     
     
195073
                           emb|CAD13586.1|
                           (AL646057) PROBABLE GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT C PROTEIN [Ralstonia solanacearum]
                           99
                           58.58
                           72.72
                           4
                           284.0
     

     






      

          
GC ORFID: 48209How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4482Glimmer Score: 

          
Start: 4440941Stop: 4441228

          
Length: 288


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4482 translation of PA4482Homolog Bit Score 187.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5563 (ORF: BLASTX 162.0), t_PA4482 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGCTTGAACGCTCCGACGTGGAAAAAATCGCCCATCTCGCCCGCCTGGGCCTGAGCGAGGCCGATCTT 
CCGCGCACCACCGAGACCCTGAACAACATCCTCGGCCTGATCGACCAGATGCAGGCGGTCGACACCAGCGGC 
GTCGAACCGCTTGCCCACCCGCTGGAAGCCACCCAGCGCCTGCGTCCGGACGCGGTCACCGAGACCGATCAC 
CGCGACGCCTACCAGACCATCGCCCCCGCCGTGGAAGAAGGTCTGTACCTGGTTCCGAAAGTCATCGAGTAA