ViewGene
Locus Name:
GeneID:
Location:

GeneID:52
TrivialName: PA14_62910
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62910
PAO1 Ortholog Locus:PA4756
Sequence Length:3222
Protein Length:1073
Start: 5615014
Stop: 5611793
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08142.1|AE004889_5 (AE004889) carbamoylphosphate synthetase large subunit [Pseudomonas aeruginosa PAO1]
Identities = 1073/1073 (100%), Positives = 1073/1073 (100%)
Sequence:
ATGCCAAAACGTACAGACATAAAGAGCATCCTGATCCTCGGTGCCGGCCCCATCGTGATTGGCCAGGCCTGC
GAATTCGACTACTCCGGCGCCCAGGCCTGCAAGGCCCTGCGCGAGGAGGGCTACCGCGTCATCCTGGTGAAC
TCCAACCCCGCGACCATCATGACCGACCCGGCGATGGCCGACGCGACCTACATCGAGCCGATCAAGTGGGCC
ACCGTGGCCAAGATCATCGAGAAGGAACGCCCCGACGCGCTGCTGCCGACCATGGGCGGCCAGACTGCGCTG
AACTGCGCCCTGGACCTGGAGCGCCACGGCGTGCTGGAGAAGTTCGGCGTGGAGATGATCGGCGCCAATGCC
GATACCATCGACAAGGCCGAGGACCGCTCGCGCTTCGACAAGGCGATGAAGGATATCGGGCTGGCCTGTCCG
CGCTCGGGCATCGCCCACAGCATGGAGGAGGCCTACGGCGTGCTCGAGCAGGTCGGCTTCCCCTGCATCATC
CGTCCGTCCTTCACCATGGGCGGCACCGGCGGCGGTATCGCCTACAACCGTGAAGAGTTCGAAGAGATCTGC
GCCCGTGGCCTCGACCTGTCGCCGACCAACGAGCTGCTGATCGACGAGTCGCTGATCGGCTGGAAGGAATAC
GAGATGGAGGTGGTCCGCGACAAGAAGGACAACTGCATCATCGTCTGCTCCATCGAGAACTTCGACCCGATG
GGCGTGCACACCGGTGACTCGATCACCGTGGCGCCGGCGCAGACCCTGACCGACAAGGAATACCAGATCATG
CGCAACGCCTCGCTGGCGGTATTGCGCGAGATCGGCGTGGAAACCGGCGGTTCCAACGTGCAGTTCGGCATC
TGCCCGAACACCGGGCGCATGGTGGTGATCGAGATGAACCCGCGGGTTTCCCGCTCCTCGGCCCTGGCCTCC
AAGGCCACCGGTTTCCCGATCGCCAAGATCGCCGCCAAGCTGGCGGTCGGCTACACCCTCGACGAACTGCAG
AACGACATCACCGGCGGTCGCACCCCGGCGTCGTTCGAGCCGGCCATCGACTACGTGGTGACCAAGATCCCG
CGCTTCGCCTTCGAGAAGTTCCCGAAAGCCGATGCCCGCCTGACCACCCAGATGAAGTCGGTCGGCGAAGTC
ATGGCCATCGGCCGGACCTTCCAGGAGTCGGTGCAGAAAGCCTTGCGTGGCCTGGAAGTCGGCGCCACCGGC
TTCGATCCGAAGCTCGACCTGAACGACCCGGAGGCCGACAGCATCCTCAAGCGCGAGCTGACCGTGCCCAGC
GCCGAGCGTGTCTGGTACGTCGCCGACGCCTTCCGCGCCGGCAAGAGCGTCGAGGAAGTGTTCGAGCTGACC
CGCATCGACGAATGGTTCCTGGTGCAGATCGAGGACCTGGTCAAGGACGAGGAGAAGGTCAAGACCCTCGGC
CTGTCCTCCATCGATCGCGAGCTGATGTACAAGCTCAAGCGCAAGGGCTTCTCCGACGCGCGCCTGGCCAAG
CTGCTAGGCGTCACCGAGAAGAACCTGCGCAGCCATCGCCACAAGCTGAAGGTGCTGCCGGTTTACAAGCGT
GTCGATACCTGCGCCGCCGAGTTCGCCACCGACACCGCCTACATGTATTCGACCTACGAGGAAGAGTGCGAG
GCCAACCCGTCGAGCCGCGAGAAGATCATGATCCTCGGCGGCGGTCCGAACCGCATCGGCCAGGGCATCGAG
TTCGACTACTGCTGCGTGCACGCGGCGCTGGCGATGCGCGAAGACGGCTACGAGACCATCATGGTCAACTGC
AACCCGGAAACCGTCTCCACCGACTACGACACCTCCGACCGCCTGTACTTCGAGCCGGTGACCCTCGAGGAC
GTCCTTGAGATCGTCCGCGTCGAGCAGCCCAAGGGCGTGATCGTCCAGTACGGCGGCCAGACCCCGCTGAAG
CTCTGCCGCGCCCTCGAAGAGGCCGGCGTGCCGATCATCGGCACCAGCCCGGACGCCATCGACCGTGCCGAA
GACCGCGAGCGCTTCCAGCAGATGGTCCAGCGCCTGAACCTGCGCCAGCCGGCCAACGCCACCGCGCGCAGC
GAAGACGAGGCCCTGGCCGCGTCCAAGGCCATCGGTTATCCGCTGGTGGTACGCCCGTCCTACGTGCTGGGC
GGGCGGGCGATGGAAATCGTCTACCAGGAAGAAGAACTCAAGCGCTACATGCGCGAGGCGGTGCAGGTTTCC
AACGACAGCCCGGTGCTGCTCGACCACTTCCTCAACTGCGCCATCGAAGTCGACATCGACGCGGTATGCGAC
GGCGAGATCGTGGTGATCGGCGCGATCATGCAGCATATCGAGCAGGCTGGCGTTCACTCCGGCGACTCCGCC
TGTTCGCTGCCGCCGTACTCGCTGCCGCAGCACATCCAGGACGAGATCCGCGAGCAGGTCAAGAAAATGGCC
CTGGAGCTTGGCGTGGTCGGCCTGATGAACGTGCAGATGGCGGTGCAGGGCGAGGATATCTTCGTCATCGAG
GTCAATCCGCGCGCCTCGCGTACCGTGCCGTTCGTCTCCAAGTGCGTCGGCGAGTCGCTGGCCAAGGTCGCC
GCCCGCGTCATGGCCGGCAAGACCCTGGCCGAGGTCGGCTTCACCCAGGAAATCATCCCGCCGTTCTTCAGC
GTCAAGGAAGCGGTGTTCCCGTTCGCCAAGTTCCCCGGCGTCGATCCGATCCTCGGCCCGGAAATGAAGTCC
ACTGGTGAAGTGATGGGCGTCGGTGATAGCTTTGCCGAAGCCTTTGCCAAGGCTCAGTTGGGGGCCAGCGAG
ATCCTCCCGACCGCCGGTTGCGCCTTCATCAGCGTCCGCGAAGACGACAAGCCGTTCGCCGCCCAGGTGGCT
GGCGACCTGGTCGCGCTGGGCTTCGAAGTGGTAGCCACCGCCGGTACCGCGCGTGTCATCGAAGCCGCAGGC
CTGCCGGTGCGTCGCGTGAACAAGGTGACCGAAGGTCGCCCGCACGTGGTGGACATGATCAAGAACGACGAG
GTCACCCTGATCATCAACACCACCGAGGGCCGGCAGTCCATCGCTGACTCCTACTCCATTCGTCGAAACGCC
CTGCAGCACAAGATCTGCTGCACCACCACCATTGCGGGTGGGCAGGCGATCTGTGAGGCGCTCAAGTTCGGT
CCCGAGAAGACCGTTCGGCGTCTGCAGGATCTCCACGCAGGAATCAAGGCATGA
Translation:
MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPAMADATYIEPIKWA
TVAKIIEKERPDALLPTMGGQTALNCALDLERHGVLEKFGVEMIGANADTIDKAEDRSRFDKAMKDIGLACP
RSGIAHSMEEAYGVLEQVGFPCIIRPSFTMGGTGGGIAYNREEFEEICARGLDLSPTNELLIDESLIGWKEY
EMEVVRDKKDNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIGVETGGSNVQFGI
CPNTGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELQNDITGGRTPASFEPAIDYVVTKIP
RFAFEKFPKADARLTTQMKSVGEVMAIGRTFQESVQKALRGLEVGATGFDPKLDLNDPEADSILKRELTVPS
AERVWYVADAFRAGKSVEEVFELTRIDEWFLVQIEDLVKDEEKVKTLGLSSIDRELMYKLKRKGFSDARLAK
LLGVTEKNLRSHRHKLKVLPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSSREKIMILGGGPNRIGQGIE
FDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLEDVLEIVRVEQPKGVIVQYGGQTPLK
LCRALEEAGVPIIGTSPDAIDRAEDRERFQQMVQRLNLRQPANATARSEDEALAASKAIGYPLVVRPSYVLG
GRAMEIVYQEEELKRYMREAVQVSNDSPVLLDHFLNCAIEVDIDAVCDGEIVVIGAIMQHIEQAGVHSGDSA
CSLPPYSLPQHIQDEIREQVKKMALELGVVGLMNVQMAVQGEDIFVIEVNPRASRTVPFVSKCVGESLAKVA
ARVMAGKTLAEVGFTQEIIPPFFSVKEAVFPFAKFPGVDPILGPEMKSTGEVMGVGDSFAEAFAKAQLGASE
ILPTAGCAFISVREDDKPFAAQVAGDLVALGFEVVATAGTARVIEAAGLPVRRVNKVTEGRPHVVDMIKNDE
VTLIINTTEGRQSIADSYSIRRNALQHKICCTTTIAGGQAICEALKFGPEKTVRRLQDLHAGIKA*
AnnotationID:62GeneID:52
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 07:07:13
Gene Name:carB
Confidence Code:1
GeneProduct:carbamoylphosphate synthetase large subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0458,pfam02786
Secondary Functional Category(ies):amino acid biosynthesis and metabolism
EC Number:6.3.5.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57949.1| (AE016872) carbamoyl-phosphate synthase, large subunit [Pseudomonas
            syringae pv. tomato str. DC3000]
          Length = 1073

 Score = 1942 bits (5030), Expect = 0.0
 Identities = 970/1073 (90%), Positives = 1024/1073 (95%)

>gb|AAN70295.1|AE016670_5 (AE016791) carbamoyl-phosphate synthase, large subunit [Pseudomonas
            putida KT2440]
          Length = 1076

 Score = 1920 bits (4973), Expect = 0.0
 Identities = 959/1073 (89%), Positives = 1019/1073 (94%)

>gb|AAN78539.1|AE016755_39 (AE016755) Carbamoyl-phosphate synthase large chain [Escherichia coli
            CFT073]
          Length = 1073

 Score = 1620 bits (4194), Expect = 0.0
 Identities = 822/1073 (76%), Positives = 919/1073 (85%), Gaps = 1/1073 (0%)








 
Structural Features:
COG0458, CarB, Carbamoylphosphate synthase large subunit (split gene in MJ) 
[Amino acid transport and metabolism / Nucleotide transport and metabolism]
CD-Length = 400 residues,  98.2% aligned
Score =  585 bits (1510), Expect = 7e-168
Identities = 279/396 (70%), Positives = 338/396 (85%), Gaps = 3/396 (0%)

pfam02786, CPSase_L_D2, Carbamoyl-phosphate synthase L chain, 
ATP binding domain.
CD-Length = 236 residues, 100.0% aligned
Score =  271 bits (695), Expect = 2e-73
Identities = 101/240 (42%), Positives = 146/240 (60%), Gaps = 7/240 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:107




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17249




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37696




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:52_cdd.html


    		




 
URL:

















    ReferenceID:37719




  
Author/Investigator(s):
        Kwon DH, Lu CD, Walthall DA, Brown TM, Houghton JE, Abdelal AT.





  
Title:
        Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists.




  
PubMed:
        8169201


MedLine:
        




    
Source:
J Bacteriol. 1994 May;176(9):2532-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8169201









           
Homologs By Global Alignment 

           

       



     
Gene ID:52
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
100
                           PA4756_tr
                           translation of PA4756
                           1073
                           99.99
                           99.99
                           0
                           5478.0
     

     
     
12035
                           gb|AAG08142.1|AE004889_5
                           (AE004889) carbamoylphosphate synthetase large subunit [Pseudomonas aeruginosa PAO1]
                           1073
                           99.99
                           99.99
                           0
                           5478.0
     

     
     
12036
                           gb|AAB39252.1|
                           (U81259) carbamoylphosphate synthetase large subunit [Pseudomonas aeruginosa]
                           1073
                           99.99
                           99.99
                           0
                           5478.0
     

     
     
12037
                           gb|AAO57949.1|
                           (AE016872) carbamoyl-phosphate synthase, large subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           1073
                           90.40
                           95.43
                           0
                           5030.0
     

     
     
12038
                           gb|AAN70295.1|AE016670_5
                           (AE016791) carbamoyl-phosphate synthase, large subunit [Pseudomonas putida KT2440]
                           1076
                           89.12
                           94.70
                           3
                           4973.0
     

     






      

          
GC ORFID: 43054How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4756Glimmer Score: 

          
Start: 4878694Stop: 4875473

          
Length: 3222


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4756 translation of PA4756Homolog Bit Score 2114.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5481 (ORF: BLASTX 1941.0), t_PA4756 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCAAAACGTACAGACATAAAGAGCATCCTGATCCTCGGTGCCGGCCCCATCGTGATTGGCCAGGCCTGC 
GAATTCGACTACTCCGGCGCCCAGGCCTGCAAGGCCCTGCGCGAGGAGGGCTACCGCGTCATCCTGGTGAAC 
TCCAACCCCGCGACCATCATGACCGACCCGGCGATGGCCGACGCGACCTACATCGAGCCGATCAAGTGGGCC 
ACCGTGGCCAAGATCATCGAGAAGGAACGCCCCGACGCGCTGCTGCCGACCATGGGCGGCCAGACTGCGCTG 
AACTGCGCCCTGGACCTGGAGCGCCACGGCGTGCTGGAGAAGTTCGGCGTGGAGATGATCGGCGCCAATGCC 
GATACCATCGACAAGGCCGAGGACCGCTCGCGCTTCGACAAGGCGATGAAGGATATCGGGCTGGCCTGTCCG 
CGCTCGGGCATCGCCCACAGCATGGAGGAGGCCTACGGCGTGCTCGAGCAGGTCGGCTTCCCCTGCATCATC 
CGTCCGTCCTTCACCATGGGCGGCACCGGCGGCGGTATCGCCTACAACCGTGAAGAGTTCGAAGAGATCTGC 
GCCCGTGGCCTCGACCTGTCGCCGACCAACGAGCTGCTGATCGACGAGTCGCTGATCGGCTGGAAGGAATAC 
GAGATGGAGGTGGTCCGCGACAAGAAGGACAACTGCATCATCGTCTGCTCCATCGAGAACTTCGACCCGATG 
GGCGTGCACACCGGTGACTCGATCACCGTGGCGCCGGCGCAGACCCTGACCGACAAGGAATACCAGATCATG 
CGCAACGCCTCGCTGGCGGTATTGCGCGAGATCGGCGTGGAAACCGGCGGTTCCAACGTGCAGTTCGGCATC 
TGCCCGAACACCGGGCGCATGGTGGTGATCGAGATGAACCCGCGGGTTTCCCGCTCCTCGGCCCTGGCCTCC 
AAGGCCACCGGTTTCCCGATCGCCAAGATCGCCGCCAAGCTGGCGGTCGGCTACACCCTCGACGAACTGCAG 
AACGACATCACCGGCGGTCGCACCCCGGCGTCGTTCGAGCCGGCCATCGACTACGTGGTGACCAAGATCCCG 
CGCTTCGCCTTCGAGAAGTTCCCGAAAGCCGATGCCCGCCTGACCACCCAGATGAAGTCGGTCGGCGAAGTC 
ATGGCCATCGGCCGGACCTTCCAGGAGTCGGTGCAGAAAGCCTTGCGTGGCCTGGAAGTCGGCGCCACCGGC 
TTCGATCCGAAGCTCGACCTGAACGACCCGGAGGCCGACAGCATCCTCAAGCGCGAGCTGACCGTGCCCAGC 
GCCGAGCGTGTCTGGTACGTCGCCGACGCCTTCCGCGCCGGCAAGAGCGTCGAGGAAGTGTTCGAGCTGACC 
CGCATCGACGAATGGTTCCTGGTGCAGATCGAGGACCTGGTCAAGGACGAGGAGAAGGTCAAGACCCTCGGC 
CTGTCCTCCATCGATCGCGAGCTGATGTACAAGCTCAAGCGCAAGGGCTTCTCCGACGCGCGCCTGGCCAAG 
CTGCTAGGCGTCACCGAGAAGAACCTGCGCAGCCATCGCCACAAGCTGAAGGTGCTGCCGGTTTACAAGCGT 
GTCGATACCTGCGCCGCCGAGTTCGCCACCGACACCGCCTACATGTATTCGACCTACGAGGAAGAGTGCGAG 
GCCAACCCGTCGAGCCGCGAGAAGATCATGATCCTCGGCGGCGGTCCGAACCGCATCGGCCAGGGCATCGAG 
TTCGACTACTGCTGCGTGCACGCGGCGCTGGCGATGCGCGAAGACGGCTACGAGACCATCATGGTCAACTGC 
AACCCGGAAACCGTCTCCACCGACTACGACACCTCCGACCGCCTGTACTTCGAGCCGGTGACCCTCGAGGAC 
GTCCTTGAGATCGTCCGCGTCGAGCAGCCCAAGGGCGTGATCGTCCAGTACGGCGGCCAGACCCCGCTGAAG 
CTCTGCCGCGCCCTCGAAGAGGCCGGCGTGCCGATCATCGGCACCAGCCCGGACGCCATCGACCGTGCCGAA 
GACCGCGAGCGCTTCCAGCAGATGGTCCAGCGCCTGAACCTGCGCCAGCCGGCCAACGCCACCGCGCGCAGC 
GAAGACGAGGCCCTGGCCGCGTCCAAGGCCATCGGTTATCCGCTGGTGGTACGCCCGTCCTACGTGCTGGGC 
GGGCGGGCGATGGAAATCGTCTACCAGGAAGAAGAACTCAAGCGCTACATGCGCGAGGCGGTGCAGGTTTCC 
AACGACAGCCCGGTGCTGCTCGACCACTTCCTCAACTGCGCCATCGAAGTCGACATCGACGCGGTATGCGAC 
GGCGAGATCGTGGTGATCGGCGCGATCATGCAGCATATCGAGCAGGCTGGCGTTCACTCCGGCGACTCCGCC 
TGTTCGCTGCCGCCGTACTCGCTGCCGCAGCACATCCAGGACGAGATCCGCGAGCAGGTCAAGAAAATGGCC 
CTGGAGCTTGGCGTGGTCGGCCTGATGAACGTGCAGATGGCGGTGCAGGGCGAGGATATCTTCGTCATCGAG 
GTCAATCCGCGCGCCTCGCGTACCGTGCCGTTCGTCTCCAAGTGCGTCGGCGAGTCGCTGGCCAAGGTCGCC 
GCCCGCGTCATGGCCGGCAAGACCCTGGCCGAGGTCGGCTTCACCCAGGAAATCATCCCGCCGTTCTTCAGC 
GTCAAGGAAGCGGTGTTCCCGTTCGCCAAGTTCCCCGGCGTCGATCCGATCCTCGGCCCGGAAATGAAGTCC 
ACTGGTGAAGTGATGGGCGTCGGTGATAGCTTTGCCGAAGCCTTTGCCAAGGCTCAGTTGGGGGCCAGCGAG 
ATCCTCCCGACCGCCGGTTGCGCCTTCATCAGCGTCCGCGAAGACGACAAGCCGTTCGCCGCCCAGGTGGCT 
GGCGACCTGGTCGCGCTGGGCTTCGAAGTGGTAGCCACCGCCGGTACCGCGCGTGTCATCGAAGCCGCAGGC 
CTGCCGGTGCGTCGCGTGAACAAGGTGACCGAAGGTCGCCCGCACGTGGTGGACATGATCAAGAACGACGAG 
GTCACCCTGATCATCAACACCACCGAGGGCCGGCAGTCCATCGCTGACTCCTACTCCATTCGTCGAAACGCC 
CTGCAGCACAAGATCTGCTGCACCACCACCATTGCGGGTGGGCAGGCGATCTGTGAGGCGCTCAAGTTCGGT 
CCCGAGAAGACCGTTCGGCGTCTGCAGGATCTCCACGCAGGAATCAAGGCATGA