ViewGene
Locus Name:
GeneID:
Location:

GeneID:5184
TrivialName: PA14_36030
AnnotatorUID: ericdeziel
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_36030
PAO1 Ortholog Locus:
Sequence Length:621
Protein Length:206
Start: 3209256
Stop: 3208636
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAK01492.1| (AF241171) paraquat-inducible protein A [Pseudomonas aeruginosa]
Identities = 206/206 (100%), Positives = 206/206 (100%) to gene #2 of PAGI-1 island and to PA4690 of PAO1.
Sequence:
ATGCGTGCGATCGATGCCGGCATTCTGGTCTGCGGCGAGTGCCACCAGTTGAATCGCGCCGACGGGAACGAG
CATCCGCGTTGCAGTCGCTGCGGCGCGAAGCTGCATCCTCGGCGGCCGAACAGCATTGCCCGTACCTGGGCG
TTGCTGATCTCGGCGATGATTCTCTACGTTCCCGCCAACCTGTTGCCGATCATGACCGTCAACATGCTCGGC
AAGGGCTCCCCGGCGACCATCATGGGCGGGGTCGTGACCCTGATCCAGGCCGACATGTTTCCCATCGCCCTG
GTGGTGTTCGTCGCCAGTATCGTCGTCCCCACCTTCAAGCTGGTCGGCATCGCCCTGTTGCTCTATTCGGTG
CAGCGGCACCAGCCGATGTCGGCCCGCCAGCGAATCATGATGTACCGCTTCATCGAATGGGTCGGGCGCTGG
TCGATGCTCGATATCTTCGTCATCGCCATCCTCGTGGCGCTGGTCAATTTCGGCAACCTGGCGAGCATCGAG
GCCAATCTCGGCGCCGCCGCCTTCGCCAGCGTGGTGGTGCTCACCATGCTCGCCGCCGTGACGTTCGACCCC
CGACTTATCTGGGACAACACCAATCTGGACGACGAGAATGAGTGA
Translation:
MRAIDAGILVCGECHQLNRADGNEHPRCSRCGAKLHPRRPNSIARTWALLISAMILYVPANLLPIMTVNMLG
KGSPATIMGGVVTLIQADMFPIALVVFVASIVVPTFKLVGIALLLYSVQRHQPMSARQRIMMYRFIEWVGRW
SMLDIFVIAILVALVNFGNLASIEANLGAAAFASVVVLTMLAAVTFDPRLIWDNTNLDDENE*
AnnotationID:5189GeneID:5184
AnnotatorUID: danlee
Modification Date/Time: 2005-10-11 15:03:45
Gene Name:PAGI-1(2)
Confidence Code:4
GeneProduct:paraquat-inducible protein A
Cell Localization:(4) Inner membrane protein
Synonyms:
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(14) Hypothetical, unclassified, unknown
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam04403
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|12698378|gb|AAK01492.1|  paraquat-inducible protein A [Pseudomonas aeruginosa]
     Length=237
     Score =  409 bits (1051),  Expect = 3e-113
     Identities = 206/206 (100%), Positives = 206/206 (100%), Gaps = 0/206 (0%)

gi|68346856|gb|AAY94462.1|  PqiA family protein VC1500 [Pseudomonas fluorescens Pf-5]
     Length=207
     Score =  326 bits (836),  Expect = 3e-88
     Identities = 160/207 (77%), Positives = 186/207 (89%), Gaps = 1/207 (0%)








 
Structural Features:
pfam04403, PqiA, Paraquat-inducible protein A. This is predicted to be a family of integral 
membrane proteins, possibly located in the inner membrane. 
             CD-Length = 207 residues,  99.5% aligned
             Score =  234 bits (599), Expect = 5e-63

COG2995, PqiA, Uncharacterized paraquat-inducible protein A [Function unknown].
             CD-Length = 418 residues, only  47.4% aligned
             Score =  219 bits (560), Expect = 1e-58






   
Genomic Context:
Predicted gene #2 of the PAGI-1 island absent in PAO1 but present in a majority of pathogenic P. aeruginosa isolates.   
The PA14 island at this locus, from PA14_35860 to PA14_36030, corresponds to PAGI-1 genes 17 through 2, respectively.






   
Comment:
The first half of the PA14 island (from PA14_35700 through PA14_35850) diverges from PAGI-1 such that 
genes 18 through 51 are absent.  PA14 has 31 ORFs in 35368 bp (from PA14_35700  to PA14_36030), 
whereas PAGI-1 contains 51 ORFs in 51300 bp.


















    ReferenceID:10368




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:16932




  
Author/Investigator(s):
        Liang X, Pham XQ, Olson MV, Lory S.





  
Title:
        Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa.




  
PubMed:
        11208781


MedLine:
        




    
Source:






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11208781&dopt=Abstract

















    ReferenceID:27512




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:5184
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
194529
                           gb|AAK01492.1|
                           (AF241171) paraquat-inducible protein A [Pseudomonas aeruginosa]
                           237
                           86.91
                           86.91
                           31
                           1051.0
     

     
     
194530
                           gb|AAQ60219.1|
                           (AE016919) paraquat-inducible protein A [Chromobacterium violaceum ATCC 12472]
                           207
                           51.20
                           64.25
                           2
                           491.0
     

     
     
194553
                           emb|CAD14132.1|
                           (AL646060) HYPOTHETICAL TRANSMEMBRANE PROTEIN [Ralstonia solanacearum]
                           247
                           37.65
                           51.01
                           41
                           419.0
     

     
     
194533
                           emb|CAE43027.1|
                           (BX640419) putative exported protein [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           399
                           23.55
                           34.58
                           194
                           469.0
     

     
     
194535
                           dbj|BAB34457.1|
                           (AP002554) paraquat-inducible protein A [Escherichia coli O157:H7]
                           420
                           21.66
                           31.42
                           217
                           461.0
     

     






      

          
GC ORFID: 48186How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: psyr_15may02_Scaffold11_revised_gene1863Glimmer Score: 

          
Start: 1744660Stop: 1745280

          
Length: 621


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: psyr_15may02_Scaffold11_revised_gene1863 related to: gi|12698378|gb|AAK01492.1| (AF241171) paraquat-inducible protein A [Pseudomonas aeruginosa]Homolog Bit Score 191.0

          
Other Homologs:  psyr_15may02_Scaffold11_revised_gene1863 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTGCGATCGATGCCGGCATTCTGGTCTGCGGCGAGTGCCACCAGTTGAATCGCGCCGACGGGAACGAG 
CATCCGCGTTGCAGTCGCTGCGGCGCGAAGCTGCATCCTCGGCGGCCGAACAGCATTGCCCGTACCTGGGCG 
TTGCTGATCTCGGCGATGATTCTCTACGTTCCCGCCAACCTGTTGCCGATCATGACCGTCAACATGCTCGGC 
AAGGGCTCCCCGGCGACCATCATGGGCGGGGTCGTGACCCTGATCCAGGCCGACATGTTTCCCATCGCCCTG 
GTGGTGTTCGTCGCCAGTATCGTCGTCCCCACCTTCAAGCTGGTCGGCATCGCCCTGTTGCTCTATTCGGTG 
CAGCGGCACCAGCCGATGTCGGCCCGCCAGCGAATCATGATGTACCGCTTCATCGAATGGGTCGGGCGCTGG 
TCGATGCTCGATATCTTCGTCATCGCCATCCTCGTGGCGCTGGTCAATTTCGGCAACCTGGCGAGCATCGAG 
GCCAATCTCGGCGCCGCCGCCTTCGCCAGCGTGGTGGTGCTCACCATGCTCGCCGCCGTGACGTTCGACCCC 
CGACTTATCTGGGACAACACCAATCTGGACGACGAGAATGAGTGA