ViewGene
Locus Name:
GeneID:
Location:

GeneID:5179
TrivialName: PA14_28980
AnnotatorUID: urbach
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_28980
PAO1 Ortholog Locus:
Sequence Length:951
Protein Length:316
Start: 2505374
Stop: 2506324
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: 
gi|23105555|ref|ZP_00092011.1|   COG3712: Fe2+-dicitrate sensor, membrane component 
[Azotobacter vinelandii] Length = 311
Identities = 163/311 (52%), Positives = 209/311 (67%), Gaps = 4/311 (1%)

emb|CAD85013.1| (BX321859) putative transmembrane sensor [Nitrosomonas europaea ATCC 19718]
Identities = 130/319 (40%), Positives = 178/319 (55%), Gaps = 18/319 (5%)
Sequence:
CTGCAAGGACTGACCATGGAACTGGAGATCGAGGTGCTGGAGGCCGCCGCCACCTGGTACGTGCGCTTCGGC
GCGGAACCGCCGAACGATGGCGAGCGCCAGGCCTGGCGCGAATGGCTGGAGGCCGACCCGCGACACCGTCGG
GCCTGGGAGCGTATCGAGCGCTTGCAGGGCCAATGGGCGGCGCTGCCGGCGGAGACCTCCTTGCACACGCTG
GCAGGCGTGCGTGCGCGGCGCCGAGCGCTGCTCAAGACGCTCGCCCTGCTGGCCTGCGCCGCGGGTAGCGGC
TGGCTGGCGAGCGGCGGCCTGCCTTACCGTGCGCTCCTCGCCGGGGAGCGCACGGGCGTCGGCGAACGCCGC
CAACTGCGCCTGGCGGACGGTACCCGGGTCGACCTGAGCAGCGCCACCGCGCTGGACCTGGCCTACGACGAA
AGCCTGCGCCAGGCGTACCTGTGGCGGGGCGAGATCCTCGTGGAAAGCGCGCAGAGCGCTCGCCCGTTCCTC
GTGCACACCGCACAAGGCAGCGTGCGCGCGCTGGGAACCCGTTTCAGCGTGCGTTGCGAGGGGCCGCGCACG
CGCGTTTGCGTCCTCAGCCATGCAGTGGAGCTACGCCCGTTGCAAACGCCGACCCGGCCGATGCGCCTGCCA
GCCGGCCAGCAGGCTCTCTTCGACGAGCGACGCGGCTACTCGTCGACGCCCTTGCGCGAAGGCGAAGGCAGT
TGGACGCAGGGCGTGCTGACGGTGGTGGACTGGCCGCTGGGCGAGTTCATCGCCGAGCTGTCCCGCTATCGC
ACCGGGGTGTTGCGCTGCGCCGCCGAGGTGGCGGAGCTGCGCCTTTCCGGCGCCTACCGCCTGGACCGGATC
GACGCGGTCCTGCAGAACCTGGGCAAGTCGCTGCCGGTGCGCGTGCGCTACCTGACGCGTTACTGGGTCACC
GTCGAGTCGGTCTGA
Translation:
MQGLTMELEIEVLEAAATWYVRFGAEPPNDGERQAWREWLEADPRHRRAWERIERLQGQWAALPAETSLHTL
AGVRARRRALLKTLALLACAAGSGWLASGGLPYRALLAGERTGVGERRQLRLADGTRVDLSSATALDLAYDE
SLRQAYLWRGEILVESAQSARPFLVHTAQGSVRALGTRFSVRCEGPRTRVCVLSHAVELRPLQTPTRPMRLP
AGQQALFDERRGYSSTPLREGEGSWTQGVLTVVDWPLGEFIAELSRYRTGVLRCAAEVAELRLSGAYRLDRI
DAVLQNLGKSLPVRVRYLTRYWVTVESV*
AnnotationID:5184GeneID:5179
AnnotatorUID: danlee
Modification Date/Time: 2005-10-12 12:12:21
Gene Name:
Confidence Code:4
GeneProduct:Possible Fe2+-dicitrate sensor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam04773, COG3712
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|67088448|gb|EAM07914.1|  FecR protein [Azotobacter vinelandii AvOP]
     Length=311
     Score =  295 bits (756),  Expect = 1e-78
     Identities = 163/311 (52%), Positives = 209/311 (67%), Gaps = 4/311 (1%)

gi|68343959|gb|AAY91565.1|  sigma factor regulatory protein FecR [Pseudomonas fluorescens Pf-5]
     Length=323
     Score =  228 bits (580),  Expect = 3e-58
     Identities = 126/307 (41%), Positives = 180/307 (58%), Gaps = 3/307 (0%)

gi|68347952|gb|AAY95558.1|  sigma factor regulatory protein, FecR/PupR family [Pseudomonas fluorescens Pf-5]
     Length=317
     Score =  220 bits (560),  Expect = 6e-56
     Identities = 130/306 (42%), Positives = 184/306 (60%), Gaps = 6/306 (1%)








 
Structural Features:
pfam04773, FecR, FecR protein. FecR is involved in regulation of iron dicitrate transport. In the absence of 
citrate FecR inactivates FecI. FecR is probably a sensor that recognises iron dicitrate in the periplasm.
             CD-Length = 323 residues,  97.5% aligned
             Score =  197 bits (501), Expect = 2e-51

COG3712, FecR, Fe2+-dicitrate sensor, membrane component.
             CD-Length = 322 residues,  98.4% aligned
             Score =  180 bits (459), Expect = 1e-46






   
Genomic Context:






   
Comment:


















    ReferenceID:15264




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:16957




  
Author/Investigator(s):
        Jonathan Urbach





  
Title:
        NCBI Conserved Domain Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:wrpsb.cgi


    		




 
URL:

















    ReferenceID:16958




  
Author/Investigator(s):
        Jonathan Urbach





  
Title:
        Blast against NR




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Blast (text/plain)







    
Data:Blast.cgi


    		




 
URL:

















    ReferenceID:27502




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:5179
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7591
                           PA1911_tr
                           translation of PA1911
                           333
                           39.63
                           52.25
                           34
                           495.0
     

     
     
194519
                           gb|AAG05299.1|AE004617_3
                           (AE004617) probable transmembrane sensor [Pseudomonas aeruginosa PAO1]
                           333
                           39.63
                           52.25
                           34
                           495.0
     

     
     
194518
                           gb|AAN68693.1|AE016498_6
                           (AE016785) transmembrane sensor, putative [Pseudomonas putida KT2440]
                           326
                           38.65
                           56.13
                           15
                           540.0
     

     
     
194517
                           emb|CAD86346.1|
                           (BX321864) putative transmembrane sensor [Nitrosomonas europaea ATCC 19718]
                           339
                           38.05
                           53.39
                           24
                           549.0
     

     
     
194516
                           emb|CAD85013.1|
                           (BX321859) putative transmembrane sensor [Nitrosomonas europaea ATCC 19718]
                           360
                           36.66
                           50.27
                           52
                           578.0
     

     






      

          
GC ORFID: 48181How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1911Glimmer Score: 

          
Start: 2448552Stop: 2447602

          
Length: 951


          
Start Codon: CTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1911 translation of PA1911Homolog Bit Score 192.0

          
Other Homologs:  t_PA1911 (ORF: GLIMMER)

          
GC ORF Sequence

           
CTGCAAGGACTGACCATGGAACTGGAGATCGAGGTGCTGGAGGCCGCCGCCACCTGGTACGTGCGCTTCGGC 
GCGGAACCGCCGAACGATGGCGAGCGCCAGGCCTGGCGCGAATGGCTGGAGGCCGACCCGCGACACCGTCGG 
GCCTGGGAGCGTATCGAGCGCTTGCAGGGCCAATGGGCGGCGCTGCCGGCGGAGACCTCCTTGCACACGCTG 
GCAGGCGTGCGTGCGCGGCGCCGAGCGCTGCTCAAGACGCTCGCCCTGCTGGCCTGCGCCGCGGGTAGCGGC 
TGGCTGGCGAGCGGCGGCCTGCCTTACCGTGCGCTCCTCGCCGGGGAGCGCACGGGCGTCGGCGAACGCCGC 
CAACTGCGCCTGGCGGACGGTACCCGGGTCGACCTGAGCAGCGCCACCGCGCTGGACCTGGCCTACGACGAA 
AGCCTGCGCCAGGCGTACCTGTGGCGGGGCGAGATCCTCGTGGAAAGCGCGCAGAGCGCTCGCCCGTTCCTC 
GTGCACACCGCACAAGGCAGCGTGCGCGCGCTGGGAACCCGTTTCAGCGTGCGTTGCGAGGGGCCGCGCACG 
CGCGTTTGCGTCCTCAGCCATGCAGTGGAGCTACGCCCGTTGCAAACGCCGACCCGGCCGATGCGCCTGCCA 
GCCGGCCAGCAGGCTCTCTTCGACGAGCGACGCGGCTACTCGTCGACGCCCTTGCGCGAAGGCGAAGGCAGT 
TGGACGCAGGGCGTGCTGACGGTGGTGGACTGGCCGCTGGGCGAGTTCATCGCCGAGCTGTCCCGCTATCGC 
ACCGGGGTGTTGCGCTGCGCCGCCGAGGTGGCGGAGCTGCGCCTTTCCGGCGCCTACCGCCTGGACCGGATC 
GACGCGGTCCTGCAGAACCTGGGCAAGTCGCTGCCGGTGCGCGTGCGCTACCTGACGCGTTACTGGGTCACC 
GTCGAGTCGGTCTGA