ViewGene
Locus Name:
GeneID:
Location:

GeneID:5172
TrivialName: PA14_64350
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64350
PAO1 Ortholog Locus:PA4865
Sequence Length:303
Protein Length:100
Start: 5734271
Stop: 5734573
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08250.1|AE004900_4 (AE004900) urease gamma subunit [Pseudomonas aeruginosa PAO1]
Identities = 99/100 (99%), Positives = 100/100 (100%)
Sequence:
ATGGACTTGTCCCCCCGCGAGAAAGACAAGCTGCTGATCTTCACCGCCGGCCTGCTCGCCGAGCGCCGGCTG
GCACGCGGTCTCAAGCTGAACTACCCGGAGGCGGTGGCGCTGATCTCCGCCGCCCTGCTGGAGGGCGCGCGC
GACGGCCGCAGCGTCGCCGAACTGATGCACTACGGCACCACCCTGCTGAGCCGCGAGCAGGTGATGGAGGGC
GTGCCGGAGATGATCCCGGACATCCAGGTGGAGGCCACCTTCCCCGACGGCACCAAGCTGGTGACCGTCCAC
CAGCCCATCGCCTGA
Translation:
MDLSPREKDKLLIFTAGLLAERRLARGLKLNYPEAVALISAALLEGARDGRSVAELMHYGTTLLSREQVMEG
VPEMIPDIQVEATFPDGTKLVTVHQPIA*
AnnotationID:5177GeneID:5172
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 06:06:56
Gene Name:ureA
Confidence Code:2
GeneProduct:urease gamma subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00547
Secondary Functional Category(ies):
EC Number:3.5.1.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58320.1| (AE016873) urease, gamma subunit [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 100

 Score =  179 bits (455), Expect = 2e-45
 Identities = 87/100 (87%), Positives = 99/100 (99%)

>emb|CAD15737.1| (AL646067) PROBABLE UREASE (GAMMA SUBUNIT) PROTEIN [Ralstonia
          solanacearum]
          Length = 100

 Score =  169 bits (428), Expect = 3e-42
 Identities = 84/99 (84%), Positives = 94/99 (94%)

>dbj|BAA17850.1| (D90909) urease gamma subunit [Synechocystis sp. PCC 6803]
          Length = 100

 Score =  169 bits (428), Expect = 3e-42
 Identities = 83/99 (83%), Positives = 94/99 (94%)








 
Structural Features:
pfam00547, Urease_gamma, Urease, gamma subunit
CD-Length = 99 residues, 100.0% aligned
Score =  144 bits (365), Expect = 2e-36
Identities = 64/99 (64%), Positives = 81/99 (81%)






   
Genomic Context:






   
Comment:


















    ReferenceID:10344




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:27488




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35195




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:5172_cdd.html


    		




 
URL:

















    ReferenceID:35956




  
Author/Investigator(s):
        Maeda M, Hidaka M, Nakamura A, Masaki H, Uozumi T.





  
Title:
        Cloning, sequencing, and expression of thermophilic Bacillus sp. strain TB-90 urease gene complex in Escherichia coli.




  
PubMed:
        8288539


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(2):432-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8288539

















    ReferenceID:35960




  
Author/Investigator(s):
        Jones BD, Mobley HL.





  
Title:
        Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease.




  
PubMed:
        2687233


MedLine:
        




    
Source:
J Bacteriol. 1989 Dec;171(12):6414-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2687233

















    ReferenceID:35964




  
Author/Investigator(s):
        Mulrooney SB, Hausinger RP.





  
Title:
        Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.




  
PubMed:
        2211515


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5837-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2211515

















    ReferenceID:35969




  
Author/Investigator(s):
        Zhu J, Teng CH, Chang CF, Chang CD, Simpson KW, Wei C, McDonough P, McDonough S, Chang YF.





  
Title:
        Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster.




  
PubMed:
        12652903


MedLine:
        




    
Source:
DNA Seq. 2002 Dec;13(6):321-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12652903









           
Homologs By Global Alignment 

           

       



     
Gene ID:5172
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7584
                           PA4865_tr
                           translation of PA4865
                           100
                           99.00
                           99.99
                           0
                           492.0
     

     
     
194325
                           gb|AAG08250.1|AE004900_4
                           (AE004900) urease gamma subunit [Pseudomonas aeruginosa PAO1]
                           100
                           99.00
                           99.99
                           0
                           492.0
     

     
     
194326
                           gb|AAO58320.1|
                           (AE016873) urease, gamma subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           100
                           87.00
                           99.00
                           0
                           455.0
     

     
     
194327
                           emb|CAD15737.1|
                           (AL646067) PROBABLE UREASE (GAMMA SUBUNIT) PROTEIN [Ralstonia solanacearum]
                           100
                           84.00
                           94.00
                           0
                           428.0
     

     
     
194329
                           emb|CAA74062.1|
                           (Y13732) urease, structural subunit [Ralstonia eutropha]
                           100
                           83.00
                           92.00
                           0
                           421.0
     

     






      

          
GC ORFID: 48174How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4865Glimmer Score: 

          
Start: 4997952Stop: 4998254

          
Length: 303


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4865 translation of PA4865Homolog Bit Score 194.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5803 (ORF: BLASTX 179.0), t_PA4865 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGACTTGTCCCCCCGCGAGAAAGACAAGCTGCTGATCTTCACCGCCGGCCTGCTCGCCGAGCGCCGGCTG 
GCACGCGGTCTCAAGCTGAACTACCCGGAGGCGGTGGCGCTGATCTCCGCCGCCCTGCTGGAGGGCGCGCGC 
GACGGCCGCAGCGTCGCCGAACTGATGCACTACGGCACCACCCTGCTGAGCCGCGAGCAGGTGATGGAGGGC 
GTGCCGGAGATGATCCCGGACATCCAGGTGGAGGCCACCTTCCCCGACGGCACCAAGCTGGTGACCGTCCAC 
CAGCCCATCGCCTGA