ViewGene
Locus Name:
GeneID:
Location:

GeneID:517
TrivialName: PA14_50140
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_50140
PAO1 Ortholog Locus:PA1101
Sequence Length:1797
Protein Length:598
Start: 4457526
Stop: 4455730
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04490.1|AE004540_10 (AE004540) Flagella M-ring outer membrane protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 598/598 (100%), Positives = 598/598 (100%)
Sequence:
ATGGCCGATGCACTGATCGACAGCCAGGTTCCCGCCAAGAGCGGCGGTGCCGGGATGCTGAAGAAATCCTTC
CCGGGACTGTCGTTCCTCGACAACCTTTCCGAGATGACCATGCTGCGCCAGATCGGCCTGCTGGTCGGTCTC
GCCGCCAGCGTGGCGATCGGCTTCGCCGTCGTGCTCTGGTCCCAGCAGCCGGACTACAAGCCGCTCTATGGC
AGCCTCAACGGGGTCGACGCGAACCGTGTGGTGGAAGCGCTGACCGCGGCGGACATTCCCTACAAGGTCGAA
CCCAACTCCGGCGCGCTGCTGGTCAAGGCCGACGACCTCGGCCGGGCACGCATGAAGGTGGCCTCCGCCGGC
GTGGCGCCGACCGACAACAACGTCGGTTTCGAGATCCTCGACAAGGAACAGGCGCTGGGCACCAGCCAGTTC
ATGGAAGCCACCAACTATCGCCGCGGCCTGGAAGGCGAGCTGGCGCGCACCGTGTCGAGCCTGAACAACGTC
AAGGCCGCCCGCGTGCACCTGGCGATCCCGAAGAGTTCGGTGTTCGTCCGCGACGACCGCAAGCCCAGCGCC
TCGGTGCTGGTCGAGCTGTATCCGGGGCGCAGCCTGGAGCCGAGCCAGGTGATGGCGATCGTCAACCTGGTC
GCCACCAGTGTGCCGGAGCTGGACAAGTCGCAGGTCACCGTGGTCGACCAGAAGGGCAACCTGCTCTCCGAC
CAGCAGGAGCTCTCCGAGCTGACCATGGCCGGCAAGCAGTTCGACTTCACCCGGCGCATGGAAGGCCTGCTC
ACCCAGCGCGTGCACAATATCCTGCAACCTGTGCTGGGCAACGGTCGCTACAAGGCCGAGGTGTCCGCCGAC
GTCGACTTCAGCGCGGTCGAGTCGACCTCGGAGATGTACAACCCGGACCAGCCGGCCCTGCGCAGCGAGCAA
CGCAACAACGAGGAACGGCAGAATTCGTCCGGCCCGCAGGGCGTGCCGGGGGCGCTGTCGAACCAGCCGCCG
GGCCCGGCCAGCGCGCCGCAGCAGGCCACCGCCAGCGCGCCGGCCGACTACGTCGCGCCGGGCCAGCCGCTG
AAGGACGCCAACGGCCAGACCATCATCGATCCGAAGACCGGCAAGCCGGAGCTGGCGCCGTACCCGACCGAC
AAGCGCGACCAGACTACCCGCAACTATGAACTGGACCGCTCGATCAGCTACACCAAGCAGCAGCAGGGGCGC
TTGCGCCGACTGTCGGTGGCGGTGGTGCTGGACGACCAGATGAAGGTCGACGCGAAGACCGGCGAGGTCAGC
CACCAGCCCTGGAGCGCCGACGAACTGGCGCGCTTCACCCGCCTGGTACAGGATTCGGTGGGCTACGACGCC
AGCCGCGGCGACAGCGTCAGCGTGATCAACGCGCCGTTCGCCCCGGCCCAGGCCGAAGAGATCGACAGCATT
CCGTTCTACTCGCAGCCGTGGTTCTGGGACATCGTCAAGCAGGTGCTCGGCGTGCTGTTCATCCTGGTCCTG
GTGTTCGGCGTGCTGCGTCCGGTGCTGAGCAACATCACCGGCGGCGGCAAGGGCAAGTCGCTGGCCGGCGGC
GGCGGTCGCGACGGCGACCTGGCGCTGGGCGAGAGTGGCCTGGAAGGCTCGCTGGCCGACGATCGGGTGAGC
ATCGGCGGGCCGTCGAGCATCCTCCTGCCGAGCCCCACGGAGGGGTATGATGCGCAATTGAACGCGATCAAG
AACCTGGTCGCACAGGACCCGGGCCGCGTCGCCCAGGTCGTGAAAGAGTGGATCAACGCCGATGAGTAA
Translation:
MADALIDSQVPAKSGGAGMLKKSFPGLSFLDNLSEMTMLRQIGLLVGLAASVAIGFAVVLWSQQPDYKPLYG
SLNGVDANRVVEALTAADIPYKVEPNSGALLVKADDLGRARMKVASAGVAPTDNNVGFEILDKEQALGTSQF
MEATNYRRGLEGELARTVSSLNNVKAARVHLAIPKSSVFVRDDRKPSASVLVELYPGRSLEPSQVMAIVNLV
ATSVPELDKSQVTVVDQKGNLLSDQQELSELTMAGKQFDFTRRMEGLLTQRVHNILQPVLGNGRYKAEVSAD
VDFSAVESTSEMYNPDQPALRSEQRNNEERQNSSGPQGVPGALSNQPPGPASAPQQATASAPADYVAPGQPL
KDANGQTIIDPKTGKPELAPYPTDKRDQTTRNYELDRSISYTKQQQGRLRRLSVAVVLDDQMKVDAKTGEVS
HQPWSADELARFTRLVQDSVGYDASRGDSVSVINAPFAPAQAEEIDSIPFYSQPWFWDIVKQVLGVLFILVL
VFGVLRPVLSNITGGGKGKSLAGGGGRDGDLALGESGLEGSLADDRVSIGGPSSILLPSPTEGYDAQLNAIK
NLVAQDPGRVAQVVKEWINADE*
AnnotationID:526GeneID:517
AnnotatorUID: danlee
Modification Date/Time: 2004-06-23 11:11:23
Gene Name:fliF
Confidence Code:1
GeneProduct:Flagella M-ring outer membrane protein precursor
Cell Localization:(6) Outer membrane protein
Synonyms:
Cell Localization Confidence Code:1
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1766
Secondary Functional Category(ies):Cell wall / LPS / capsule
EC Number:
Status:ACTIVE
Pathway:
Flagella assembly
Homology:






 
Structural Features:
COG1766, FliF, Flagellar biosynthesis/type III secretory pathway lipoprotein 
[Cell motility and secretion / Intracellular trafficking and secretion]
             CD-Length = 545 residues,  97.6% aligned
             Score =  426 bits (1097), Expect = 3e-120






   
Genomic Context:
fliFGHI (Ecoli) 






   
Comment:


















    ReferenceID:1037




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18179




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32714




  
Author/Investigator(s):
        Arora SK, Ritchings BW, Almira EC, Lory S, Ramphal R.







  
Title:
        Cloning and characterization of Pseudomonas aeruginosa fliF, necessary for flagellar assembly and bacterial adherence to mucin.





  
PubMed:
        96239027


MedLine:
        




    
Source:
Infect Immun. 1996 Jun;64(6):2130-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:517
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
911
                           PA1101_tr
                           translation of PA1101
                           598
                           99.99
                           99.99
                           0
                           3018.0
     

     
     
36869
                           gb|AAG04490.1|AE004540_10
                           (AE004540) Flagella M-ring outer membrane protein precursor [Pseudomonas aeruginosa PAO1]
                           598
                           99.99
                           99.99
                           0
                           3018.0
     

     
     
36870
                           gb|AAB06801.1|
                           (L43507) flagellar MS ring [Pseudomonas aeruginosa]
                           599
                           98.83
                           98.99
                           1
                           2973.0
     

     
     
36872
                           gb|AAO55476.1|
                           (AE016862) flagellar M-ring protein FliF [Pseudomonas syringae pv. tomato str. DC3000]
                           600
                           75.50
                           86.00
                           8
                           2265.0
     

     
     
36871
                           gb|AAN69947.1|AE016633_5
                           (AE016790) flagellar basal-body M-ring protein FliF [Pseudomonas putida KT2440]
                           600
                           75.16
                           86.50
                           10
                           2284.0
     

     






      

          
GC ORFID: 43519How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1101Glimmer Score: 

          
Start: 496382Stop: 498178

          
Length: 1797


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA1101 translation of PA1101Homolog Bit Score 1167.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7918 (ORF: BLASTX 865.0), t_PA1101 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCGATGCACTGATCGACAGCCAGGTTCCCGCCAAGAGCGGCGGTGCCGGGATGCTGAAGAAATCCTTC 
CCGGGACTGTCGTTCCTCGACAACCTTTCCGAGATGACCATGCTGCGCCAGATCGGCCTGCTGGTCGGTCTC 
GCCGCCAGCGTGGCGATCGGCTTCGCCGTCGTGCTCTGGTCCCAGCAGCCGGACTACAAGCCGCTCTATGGC 
AGCCTCAACGGGGTCGACGCGAACCGTGTGGTGGAAGCGCTGACCGCGGCGGACATTCCCTACAAGGTCGAA 
CCCAACTCCGGCGCGCTGCTGGTCAAGGCCGACGACCTCGGCCGGGCACGCATGAAGGTGGCCTCCGCCGGC 
GTGGCGCCGACCGACAACAACGTCGGTTTCGAGATCCTCGACAAGGAACAGGCGCTGGGCACCAGCCAGTTC 
ATGGAAGCCACCAACTATCGCCGCGGCCTGGAAGGCGAGCTGGCGCGCACCGTGTCGAGCCTGAACAACGTC 
AAGGCCGCCCGCGTGCACCTGGCGATCCCGAAGAGTTCGGTGTTCGTCCGCGACGACCGCAAGCCCAGCGCC 
TCGGTGCTGGTCGAGCTGTATCCGGGGCGCAGCCTGGAGCCGAGCCAGGTGATGGCGATCGTCAACCTGGTC 
GCCACCAGTGTGCCGGAGCTGGACAAGTCGCAGGTCACCGTGGTCGACCAGAAGGGCAACCTGCTCTCCGAC 
CAGCAGGAGCTCTCCGAGCTGACCATGGCCGGCAAGCAGTTCGACTTCACCCGGCGCATGGAAGGCCTGCTC 
ACCCAGCGCGTGCACAATATCCTGCAACCTGTGCTGGGCAACGGTCGCTACAAGGCCGAGGTGTCCGCCGAC 
GTCGACTTCAGCGCGGTCGAGTCGACCTCGGAGATGTACAACCCGGACCAGCCGGCCCTGCGCAGCGAGCAA 
CGCAACAACGAGGAACGGCAGAATTCGTCCGGCCCGCAGGGCGTGCCGGGGGCGCTGTCGAACCAGCCGCCG 
GGCCCGGCCAGCGCGCCGCAGCAGGCCACCGCCAGCGCGCCGGCCGACTACGTCGCGCCGGGCCAGCCGCTG 
AAGGACGCCAACGGCCAGACCATCATCGATCCGAAGACCGGCAAGCCGGAGCTGGCGCCGTACCCGACCGAC 
AAGCGCGACCAGACTACCCGCAACTATGAACTGGACCGCTCGATCAGCTACACCAAGCAGCAGCAGGGGCGC 
TTGCGCCGACTGTCGGTGGCGGTGGTGCTGGACGACCAGATGAAGGTCGACGCGAAGACCGGCGAGGTCAGC 
CACCAGCCCTGGAGCGCCGACGAACTGGCGCGCTTCACCCGCCTGGTACAGGATTCGGTGGGCTACGACGCC 
AGCCGCGGCGACAGCGTCAGCGTGATCAACGCGCCGTTCGCCCCGGCCCAGGCCGAAGAGATCGACAGCATT 
CCGTTCTACTCGCAGCCGTGGTTCTGGGACATCGTCAAGCAGGTGCTCGGCGTGCTGTTCATCCTGGTCCTG 
GTGTTCGGCGTGCTGCGTCCGGTGCTGAGCAACATCACCGGCGGCGGCAAGGGCAAGTCGCTGGCCGGCGGC 
GGCGGTCGCGACGGCGACCTGGCGCTGGGCGAGAGTGGCCTGGAAGGCTCGCTGGCCGACGATCGGGTGAGC 
ATCGGCGGGCCGTCGAGCATCCTCCTGCCGAGCCCCACGGAGGGGTATGATGCGCAATTGAACGCGATCAAG 
AACCTGGTCGCACAGGACCCGGGCCGCGTCGCCCAGGTCGTGAAAGAGTGGATCAACGCCGATGAGTAA