ViewGene

Locus Name:
GeneID:
Location:

GeneID:5127
TrivialName: PA14_09220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_09220
PAO1 Ortholog Locus:PA4230
Sequence Length:306
Protein Length:101
Start: 781999
Stop: 781694
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07618.1|AE004840_4 (AE004840) salicylate biosynthesis protein PchB [Pseudomonas aeruginosa PAO1]
Identities = 99/101 (98%), Positives = 101/101 (100%)
Sequence:
ATGAAAACTCCCGAAGACTGCACCGGCCTGGCGGACATCCGCGAGGCCATCGACCAGATCGACCTGGATATC
GTCCAGGCCCTCGGCCGCCGCATGGACTACGTCAAGGCGGCGTCGCGCTTCAAGGCCAACGAGGCGGCGATT
CCGGCGCCCGAGCGGGTCGCCGCGATGCTCCCCGAGCGCGCCCGCTGGGCCGAGGAAAACGGACTCGACGCG
CCCTTCGTCGAGGGACTGTTCGCGCAGATCATCCACTGGTACATCGCCGAGCAGATCAAGTACTGGCGCCAG
ACACGGGGTGCCGCATGA
Translation:
MKTPEDCTGLADIREAIDQIDLDIVQALGRRMDYVKAASRFKANEAAIPAPERVAAMLPERARWAEENGLDA
PFVEGLFAQIIHWYIAEQIKYWRQTRGAA*
AnnotationID:5132GeneID:5127
AnnotatorUID: diggins
Modification Date/Time: 2005-06-06 09:09:00
Gene Name:pchB
Confidence Code:1
GeneProduct:salicylate biosynthesis protein PchB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:isochorismate pyruvate-lyase
Functional Category Confidence Code:5
COGs:COG1605,pfam01817
Secondary Functional Category(ies):amino acid biosynthesis and metabolism
EC Number:
Status:ACTIVE
Pathway:
Siderophore biosynthesis; salicylate biosynthesis.
Homology:
>gb|AAO07762.1|AE016811_3 (AE016811) Chorismate mutase [Vibrio vulnificus CMCP6]
          Length = 109

 Score =  118 bits (295), Expect = 8e-27
 Identities = 56/101 (55%), Positives = 71/101 (70%)

>gb|AAO56100.1| (AE016865) isochorismate pyruvate-lyase [Pseudomonas syringae pv.
          tomato str. DC3000]
          Length = 106

 Score =  109 bits (273), Expect = 3e-24
 Identities = 50/95 (52%), Positives = 67/95 (70%)

Structural Features:
COG1605, PheA, Chorismate mutase [Amino acid transport and metabolism]
CD-Length = 101 residues, 100.0% aligned
Score = 78.8 bits (194), Expect = 1e-16
Identities = 31/101 (30%), Positives = 46/101 (45%), Gaps = 2/101 (1%)

pfam01817, Chorismate_mut, Chorismate mutase.
CD-Length = 88 residues,  97.7% aligned
Score = 75.0 bits (184), Expect = 2e-15
Identities = 28/86 (32%), Positives = 42/86 (48%), Gaps = 1/86 (1%)
Genomic Context:
Comment:
ReferenceID:10254
Author/Investigator(s): GenNotator
Title:
PubMed:
MedLine:
Source:
Reference Type:BLASTP
Data:
URL:
ReferenceID:27398
Author/Investigator(s): GenNotator
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:RPSBLAST
Data:
URL:
ReferenceID:36928
Author/Investigator(s):
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:Misc (text/plain)
Data:5127_cdd.html
URL:
ReferenceID:37016
Author/Investigator(s): Serino L, Reimmann C, Baur H, Beyeler M, Visca P, Haas D.
Title: Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa.
PubMed: 7500944
MedLine:
Source:
Mol Gen Genet. 1995 Nov 15;249(2):217-28.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=96086939&dopt=Abstract
Homologs By Global Alignment
Gene ID:5127

Identity:

0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score
7535 PA4230_tr translation of PA4230 101 98.01 99.99 0 516.0
193678 gb|AAG07618.1|AE004840_4 (AE004840) salicylate biosynthesis protein PchB [Pseudomonas aeruginosa PAO1] 101 98.01 99.99 0 516.0
193679 emb|CAA57968.1| (X82644) pchB [Pseudomonas aeruginosa] 102 97.05 99.01 1 516.0
193680 gb|AAO07762.1|AE016811_3 (AE016811) Chorismate mutase [Vibrio vulnificus CMCP6] 109 51.37 65.13 8 295.0
193681 dbj|BAC97331.1| (AP005349) chorismate mutase [Vibrio vulnificus YJ016] 109 51.37 65.13 8 295.0
GC ORFID: 48129How Found: BLASTX
GC_TrimmedSeqID: 69Blast Result ID
Subject Sequence Name: t_PA4230Glimmer Score:
Start: 4171938Stop: 4172243
Length: 306
Start Codon: ATGTruncated Start:
Stop Codon: TGATruncated Stop:
Homolog: t_PA4230 translation of PA4230Homolog Bit Score 203.0
Other Homologs: t_PA4230 (ORF: GLIMMER)
GC ORF Sequence
ATGAAAACTCCCGAAGACTGCACCGGCCTGGCGGACATCCGCGAGGCCATCGACCAGATCGACCTGGATATC
GTCCAGGCCCTCGGCCGCCGCATGGACTACGTCAAGGCGGCGTCGCGCTTCAAGGCCAACGAGGCGGCGATT
CCGGCGCCCGAGCGGGTCGCCGCGATGCTCCCCGAGCGCGCCCGCTGGGCCGAGGAAAACGGACTCGACGCG
CCCTTCGTCGAGGGACTGTTCGCGCAGATCATCCACTGGTACATCGCCGAGCAGATCAAGTACTGGCGCCAG
ACACGGGGTGCCGCATGA