ViewGene
Locus Name:
GeneID:
Location:

GeneID:51
TrivialName: PA14_66400
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66400
PAO1 Ortholog Locus:PA5022
Sequence Length:3357
Protein Length:1118
Start: 5920919
Stop: 5924275
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08407.1|AE004915_2 (AE004915) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 1109/1118 (99%), Positives = 1112/1118 (99%)
Sequence:
ATGCCTGCCCTCCGCTCCCTGATCGCTACTCTTTTCCTCGGCCTCTGCCTTGCCAGTCCGCCGCTCCTCGCC
CAGTCCGAGCCGCCCTCGGCCGAAGCCGTCCAGCAAAGCCTCGACAAGCTGGCCGAACGCAAGCTCGCCGAA
GCCGACCAGAAGGTCGTCAAGGCCAGACTCGAACAGACCCTCAAGTTCCTCGCCGCGCGCGACGAAGCGCTG
CAGAGCCTGGAAGACCTGAAAAAGCGTCTGAGCGACGCGCCCCGGCAGATCGAGGATAACCAGCGCGAACTG
GAGCGCCTGAAGAAGACCCGCGAACGCCCGGTCAGCGAGCGCTACAGCGGCGAGAGCGTGGCCCGCCTGGAG
ATGCTGCTCAACGACCGCACCACCCAGCAGGCCGAGTGGCAGAAGGCCCTGGGCGAGGCCAACAGCCTGTCG
ATCACCGCCGAGACCCGCCCGGAACGAGCCCAGGCCGGCATCAGCAGCATGCAGGCACGAATCCTGGAGATC
GGCTCGCTGCTCAAGGCCGGCAAGGAAAGCGGCAAGGCGATCAACGCCGACCGTCGCGGCGAGCTGCTCGCC
GAGCAGGCCGCGCTGACCGTGCAGAGCCAGCTGTTGCGCCAGGAACTGGCCGGCAACAACCTGCTGCAGGAC
CTCGGCAAGAGCCAGCACGATCTGCTCACCGAGAAGATCTCGCGCCTGGAAAAGGAAACCCTCGACCTGCAG
GCGCTAATCAGCGAGAAGCGCCGCGAGCAGTCGGAAAAGACCGTCGCCGAGCTGTCCAAGGAAGGCGCCCAG
GGGGCCGGCACCGACAGCCTGCTGAGCCAGGAGAACGCCAAGAATCTGCGTCTCTCCGACTACCTGCTGCGC
GCCACCGACCGCCTCAACGTGCTCACCCGGCGCAACCTCGAGACCAAGCAGCAACTGGACAACCTGACCCAG
TCCAACCAGGCCCTCGAGGAACAGATCAACGTCCTGCGCGGCAGCCTGCTGCTGTCGCGCATCCTCTACAAG
CAGAAACAGGCGCTGCCGAAGATCAAGACCGACCAGAGCCTGGCCGACGAGATCGCCGACCTGCGCCTCGGC
CAGTTCGAACTGAACCAGGAACGCGACAAGCTGGCGACCCCGCAGCAATACCTGGACGACCTGCTCGCCCAG
CAGCCCAGCGAGCAGGTCACGCCGGAACTGCGCAAGGATCTCGACACCCTGCTGGCAACCCGTTCCGAGCTG
CTCGAACGGCTCAACCACGAACTCAACGCGCTGCTCAACGAAGCGATCACCTTGCAGTTGAACCAGAAGCAA
CTGCTTTCCACCAGCGAGAGCCTGCGCACCACCCTCGACGAGCAGATGTTCTGGATTCCCAGCAACCAGCCG
CTCGACCTGTCCTGGTTCAAGATGACCCCGACCCTGCTGAAGAACCAGCTCACCGAGATCCCCTGGGGCTCC
GGGGTGCGTGAACTGGGCGAAGGCCTGGTCGATCGGCCGCTGCTGTTCCTGCCGCTGTTCCTGCTGATCGCC
GCTCTGCTGTGGAAACGCCGCTACCTGTACGACAAGCTGGCGGAGCTGAACGACGACATCGGCCACTTCAAG
CGCGACAGCCAGCTGCATACCCCGCTGGCGATCCTGATCACCATCCTCCTCGCCCTGCCCGGCACCCTGACC
TTCGCCGTCGCCGGCCTGGCCCTGCTGCTGGACGCCCGCGGCCAGAACGCCACCCTCGGCAGCGCCCTGCTG
GAAATGGCCCAGGTCTGGCTGCTGTTCTACACCGCCCATCGCATCCTCACCCCGGGCGGCATCGCCGAGCGG
CATTTCCACTGGAGCCGCGAACAGGTCGGCTTCCTCGACCGGCACCTGGTCCGCCTCGGCGTTGTGGTCCTG
GCGCTGGTCGGCGTGGTGACGGTGGCCGAGCACCAGCCGGCGAGCCTGGCCGACGACGTGATCGGCATCGCC
GTGGTCCTGCTCGGCTACGCGGCCATGGCCTGGCTGCTCAGCCGCCTGCTGTTCAGCAGCCCCGGCGACGAG
CGTCCGTCGCTGATCAAGATGATCGTCGGCCTCCTCTTCACCGCGCTGCCGCTGGTGCTCTTCGTCGCGGTC
TGCTTCGGCTACTACTACACCTCGCTGAAGCTCACCGACCGTCTCATCGATACCCTCTACCTGCTGCTGCTC
TGGTTCGTCATCGAAGCCGCCTTCGTCCGCGGCCTGGGCGTGGCGGCCCGGCGCCTGGCCTACGCCCGGGCC
CTGGCGAAACGGCAGAACGCGGCGAAGGAAGGCGTCGACGGCGAGACCAACGTCGAGGAGCCGACCCTCGGC
ATCGAGCAGATCAACCAGCAATCGCTGCGGCTGATCCGCCTGGCCCTGCTGATCGGCTTCATCGTCTGCCTG
TACTGGGTCTGGGCCGACCTGATCAGCGTGATCTCCTACCTCGACAACGTCACCCTGTACCAGTACACCGCC
GGCACCGGCGAAGCGGCCACCAGCGTGCCGATCAGCCTGCGCGACTTCCTCGCGGCGCTGGCCATCGCGGTG
GTCACCGTGGCCCTGGCGCGCAACCTGCCCGGCCTGCTGGAGGTATTGGTGCTACAGCGCCTGACCCTGGCC
CAGGGCAGCGCCTACGCCACCACCACCCTGCTTTCCTATGCCATCAGCGGCATCGGCATCGTCAGCGCCCTG
TCCACCCTCGGGGTCAGCTGGGACAAGCTGCAATGGCTGGTGGCGGCGCTCTCGGTAGGCCTCGGCTTCGGC
CTGCAGGCGATCTTCTCCAACTTCGTCTCCGGCCTGATCATCCTTTTCGAGCGTCCGGTGCGAATCGGCGAC
GTGGTGACCATCGGCAACCTGTCCGGCACGGTCAGTCGGATCCGCATCCGCGCCACCACCATCACCGATTTC
GACCGCAAGGAGATCATCGTCCCGAACCAGACCTTCATCACCGGGCAACTGGTGAACTGGTCGCTGAGCGAT
ACCGTGACCCGGGTCACCATCAAGATCGGCCTGGCCTACGAGACCGACCTGCCGCTGGCGCGCAAGCTGATG
ATGCAGGCGGTCAAGGAAAACCCGCGGGTACTGCGCGACCCGGAGCCGCTGCTGTACTTCCTGACCATCAGC
GCCAGTACCTTCGACTACGAGCTGCGCTTCCACGTCCGCGAACTGGGCGACCGCAACCCCTCCACCGACGAG
ATCCTGACGAAGATCGCCACCAGCTTCCGCGAACACAAGATCGACATGGCTTTCAACCAGGTCGATGTGTTC
GTCAAGAACCTCCAGGGCCAGCAGGTCCAGCTGTTCGTCGGCGAGCCGCAGCACAGCGGCGTCGGCAATCCG
CCCGCGCTCAAGGGCCAGGACGGTCCCGCCGAACCGACCCGCTGA
Translation:
MPALRSLIATLFLGLCLASPPLLAQSEPPSAEAVQQSLDKLAERKLAEADQKVVKARLEQTLKFLAARDEAL
QSLEDLKKRLSDAPRQIEDNQRELERLKKTRERPVSERYSGESVARLEMLLNDRTTQQAEWQKALGEANSLS
ITAETRPERAQAGISSMQARILEIGSLLKAGKESGKAINADRRGELLAEQAALTVQSQLLRQELAGNNLLQD
LGKSQHDLLTEKISRLEKETLDLQALISEKRREQSEKTVAELSKEGAQGAGTDSLLSQENAKNLRLSDYLLR
ATDRLNVLTRRNLETKQQLDNLTQSNQALEEQINVLRGSLLLSRILYKQKQALPKIKTDQSLADEIADLRLG
QFELNQERDKLATPQQYLDDLLAQQPSEQVTPELRKDLDTLLATRSELLERLNHELNALLNEAITLQLNQKQ
LLSTSESLRTTLDEQMFWIPSNQPLDLSWFKMTPTLLKNQLTEIPWGSGVRELGEGLVDRPLLFLPLFLLIA
ALLWKRRYLYDKLAELNDDIGHFKRDSQLHTPLAILITILLALPGTLTFAVAGLALLLDARGQNATLGSALL
EMAQVWLLFYTAHRILTPGGIAERHFHWSREQVGFLDRHLVRLGVVVLALVGVVTVAEHQPASLADDVIGIA
VVLLGYAAMAWLLSRLLFSSPGDERPSLIKMIVGLLFTALPLVLFVAVCFGYYYTSLKLTDRLIDTLYLLLL
WFVIEAAFVRGLGVAARRLAYARALAKRQNAAKEGVDGETNVEEPTLGIEQINQQSLRLIRLALLIGFIVCL
YWVWADLISVISYLDNVTLYQYTAGTGEAATSVPISLRDFLAALAIAVVTVALARNLPGLLEVLVLQRLTLA
QGSAYATTTLLSYAISGIGIVSALSTLGVSWDKLQWLVAALSVGLGFGLQAIFSNFVSGLIILFERPVRIGD
VVTIGNLSGTVSRIRIRATTITDFDRKEIIVPNQTFITGQLVNWSLSDTVTRVTIKIGLAYETDLPLARKLM
MQAVKENPRVLRDPEPLLYFLTISASTFDYELRFHVRELGDRNPSTDEILTKIATSFREHKIDMAFNQVDVF
VKNLQGQQVQLFVGEPQHSGVGNPPALKGQDGPAEPTR*
AnnotationID:61GeneID:51
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 12:12:57
Gene Name:
Confidence Code:3
GeneProduct:putative small-conductance mechanosensitive channel protein
Cell Localization:()
Synonyms:aefA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative putative potassium efflux system
Functional Category Confidence Code:5
COGs:COG3264,pfam00924
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70632.1|AE016707_2 (AE016793) potassium efflux system protein KefA, putative
            [Pseudomonas putida KT2440]
          Length = 1102

 Score = 1370 bits (3546), Expect = 0.0
 Identities = 709/1102 (64%), Positives = 873/1102 (79%), Gaps = 3/1102 (0%)

>gb|AAO58452.1| (AE016874) potassium efflux system protein KefA, putative
            [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 1134

 Score = 1347 bits (3486), Expect = 0.0
 Identities = 694/1096 (63%), Positives = 868/1096 (79%), Gaps = 3/1096 (0%)

>emb|CAC92364.1| (AJ414155) putative potassium efflux system [Yersinia pestis CO92]
          Length = 1139

 Score =  856 bits (2212), Expect = 0.0
 Identities = 477/1065 (44%), Positives = 672/1065 (63%), Gaps = 5/1065 (0%)








 
Structural Features:
COG3264, COG3264, Small-conductance mechanosensitive channel 
[Cell envelope biogenesis, outer membrane]
CD-Length = 835 residues,  99.3% aligned
Score =  738 bits (1906), Expect = 0.0
Identities = 369/895 (41%), Positives = 492/895 (54%), Gaps = 66/895 (7%)

pfam00924, MS_channel, Mechanosensitive ion channel. 
CD-Length = 205 residues, 100.0% aligned
Score =  180 bits (459), Expect = 6e-46
Identities = 83/206 (40%), Positives = 124/206 (60%), Gaps = 5/206 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:105




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17247




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35080




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:51_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:51
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
99
                           PA5022_tr
                           translation of PA5022
                           1118
                           99.19
                           99.46
                           0
                           5495.0
     

     
     
12026
                           gb|AAG08407.1|AE004915_2
                           (AE004915) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           1118
                           99.19
                           99.46
                           0
                           5495.0
     

     
     
12027
                           gb|AAN70632.1|AE016707_2
                           (AE016793) potassium efflux system protein KefA, putative [Pseudomonas putida KT2440]
                           1119
                           63.36
                           78.01
                           18
                           3549.0
     

     
     
12028
                           gb|AAO58452.1|
                           (AE016874) potassium efflux system protein KefA, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           1142
                           61.55
                           76.88
                           32
                           3493.0
     

     
     
12030
                           emb|CAC92364.1|
                           (AJ414155) putative potassium efflux system [Yersinia pestis CO92]
                           1148
                           42.59
                           60.27
                           39
                           2220.0
     

     






      

          
GC ORFID: 43053How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5022Glimmer Score: 

          
Start: 5184602Stop: 5187958

          
Length: 3357


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5022 translation of PA5022Homolog Bit Score 2121.0

          
Other Homologs:  t_PA5022 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCTGCCCTCCGCTCCCTGATCGCTACTCTTTTCCTCGGCCTCTGCCTTGCCAGTCCGCCGCTCCTCGCC 
CAGTCCGAGCCGCCCTCGGCCGAAGCCGTCCAGCAAAGCCTCGACAAGCTGGCCGAACGCAAGCTCGCCGAA 
GCCGACCAGAAGGTCGTCAAGGCCAGACTCGAACAGACCCTCAAGTTCCTCGCCGCGCGCGACGAAGCGCTG 
CAGAGCCTGGAAGACCTGAAAAAGCGTCTGAGCGACGCGCCCCGGCAGATCGAGGATAACCAGCGCGAACTG 
GAGCGCCTGAAGAAGACCCGCGAACGCCCGGTCAGCGAGCGCTACAGCGGCGAGAGCGTGGCCCGCCTGGAG 
ATGCTGCTCAACGACCGCACCACCCAGCAGGCCGAGTGGCAGAAGGCCCTGGGCGAGGCCAACAGCCTGTCG 
ATCACCGCCGAGACCCGCCCGGAACGAGCCCAGGCCGGCATCAGCAGCATGCAGGCACGAATCCTGGAGATC 
GGCTCGCTGCTCAAGGCCGGCAAGGAAAGCGGCAAGGCGATCAACGCCGACCGTCGCGGCGAGCTGCTCGCC 
GAGCAGGCCGCGCTGACCGTGCAGAGCCAGCTGTTGCGCCAGGAACTGGCCGGCAACAACCTGCTGCAGGAC 
CTCGGCAAGAGCCAGCACGATCTGCTCACCGAGAAGATCTCGCGCCTGGAAAAGGAAACCCTCGACCTGCAG 
GCGCTAATCAGCGAGAAGCGCCGCGAGCAGTCGGAAAAGACCGTCGCCGAGCTGTCCAAGGAAGGCGCCCAG 
GGGGCCGGCACCGACAGCCTGCTGAGCCAGGAGAACGCCAAGAATCTGCGTCTCTCCGACTACCTGCTGCGC 
GCCACCGACCGCCTCAACGTGCTCACCCGGCGCAACCTCGAGACCAAGCAGCAACTGGACAACCTGACCCAG 
TCCAACCAGGCCCTCGAGGAACAGATCAACGTCCTGCGCGGCAGCCTGCTGCTGTCGCGCATCCTCTACAAG 
CAGAAACAGGCGCTGCCGAAGATCAAGACCGACCAGAGCCTGGCCGACGAGATCGCCGACCTGCGCCTCGGC 
CAGTTCGAACTGAACCAGGAACGCGACAAGCTGGCGACCCCGCAGCAATACCTGGACGACCTGCTCGCCCAG 
CAGCCCAGCGAGCAGGTCACGCCGGAACTGCGCAAGGATCTCGACACCCTGCTGGCAACCCGTTCCGAGCTG 
CTCGAACGGCTCAACCACGAACTCAACGCGCTGCTCAACGAAGCGATCACCTTGCAGTTGAACCAGAAGCAA 
CTGCTTTCCACCAGCGAGAGCCTGCGCACCACCCTCGACGAGCAGATGTTCTGGATTCCCAGCAACCAGCCG 
CTCGACCTGTCCTGGTTCAAGATGACCCCGACCCTGCTGAAGAACCAGCTCACCGAGATCCCCTGGGGCTCC 
GGGGTGCGTGAACTGGGCGAAGGCCTGGTCGATCGGCCGCTGCTGTTCCTGCCGCTGTTCCTGCTGATCGCC 
GCTCTGCTGTGGAAACGCCGCTACCTGTACGACAAGCTGGCGGAGCTGAACGACGACATCGGCCACTTCAAG 
CGCGACAGCCAGCTGCATACCCCGCTGGCGATCCTGATCACCATCCTCCTCGCCCTGCCCGGCACCCTGACC 
TTCGCCGTCGCCGGCCTGGCCCTGCTGCTGGACGCCCGCGGCCAGAACGCCACCCTCGGCAGCGCCCTGCTG 
GAAATGGCCCAGGTCTGGCTGCTGTTCTACACCGCCCATCGCATCCTCACCCCGGGCGGCATCGCCGAGCGG 
CATTTCCACTGGAGCCGCGAACAGGTCGGCTTCCTCGACCGGCACCTGGTCCGCCTCGGCGTTGTGGTCCTG 
GCGCTGGTCGGCGTGGTGACGGTGGCCGAGCACCAGCCGGCGAGCCTGGCCGACGACGTGATCGGCATCGCC 
GTGGTCCTGCTCGGCTACGCGGCCATGGCCTGGCTGCTCAGCCGCCTGCTGTTCAGCAGCCCCGGCGACGAG 
CGTCCGTCGCTGATCAAGATGATCGTCGGCCTCCTCTTCACCGCGCTGCCGCTGGTGCTCTTCGTCGCGGTC 
TGCTTCGGCTACTACTACACCTCGCTGAAGCTCACCGACCGTCTCATCGATACCCTCTACCTGCTGCTGCTC 
TGGTTCGTCATCGAAGCCGCCTTCGTCCGCGGCCTGGGCGTGGCGGCCCGGCGCCTGGCCTACGCCCGGGCC 
CTGGCGAAACGGCAGAACGCGGCGAAGGAAGGCGTCGACGGCGAGACCAACGTCGAGGAGCCGACCCTCGGC 
ATCGAGCAGATCAACCAGCAATCGCTGCGGCTGATCCGCCTGGCCCTGCTGATCGGCTTCATCGTCTGCCTG 
TACTGGGTCTGGGCCGACCTGATCAGCGTGATCTCCTACCTCGACAACGTCACCCTGTACCAGTACACCGCC 
GGCACCGGCGAAGCGGCCACCAGCGTGCCGATCAGCCTGCGCGACTTCCTCGCGGCGCTGGCCATCGCGGTG 
GTCACCGTGGCCCTGGCGCGCAACCTGCCCGGCCTGCTGGAGGTATTGGTGCTACAGCGCCTGACCCTGGCC 
CAGGGCAGCGCCTACGCCACCACCACCCTGCTTTCCTATGCCATCAGCGGCATCGGCATCGTCAGCGCCCTG 
TCCACCCTCGGGGTCAGCTGGGACAAGCTGCAATGGCTGGTGGCGGCGCTCTCGGTAGGCCTCGGCTTCGGC 
CTGCAGGCGATCTTCTCCAACTTCGTCTCCGGCCTGATCATCCTTTTCGAGCGTCCGGTGCGAATCGGCGAC 
GTGGTGACCATCGGCAACCTGTCCGGCACGGTCAGTCGGATCCGCATCCGCGCCACCACCATCACCGATTTC 
GACCGCAAGGAGATCATCGTCCCGAACCAGACCTTCATCACCGGGCAACTGGTGAACTGGTCGCTGAGCGAT 
ACCGTGACCCGGGTCACCATCAAGATCGGCCTGGCCTACGAGACCGACCTGCCGCTGGCGCGCAAGCTGATG 
ATGCAGGCGGTCAAGGAAAACCCGCGGGTACTGCGCGACCCGGAGCCGCTGCTGTACTTCCTGACCATCAGC 
GCCAGTACCTTCGACTACGAGCTGCGCTTCCACGTCCGCGAACTGGGCGACCGCAACCCCTCCACCGACGAG 
ATCCTGACGAAGATCGCCACCAGCTTCCGCGAACACAAGATCGACATGGCTTTCAACCAGGTCGATGTGTTC 
GTCAAGAACCTCCAGGGCCAGCAGGTCCAGCTGTTCGTCGGCGAGCCGCAGCACAGCGGCGTCGGCAATCCG 
CCCGCGCTCAAGGGCCAGGACGGTCCCGCCGAACCGACCCGCTGA