ViewGene
Locus Name:
GeneID:
Location:

GeneID:5042
TrivialName: PA14_69810
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69810
PAO1 Ortholog Locus:PA5288
Sequence Length:339
Protein Length:112
Start: 6226035
Stop: 6225697
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08673.1|AE004941_6 (AE004941) nitrogen regulatory protein P-II 2 [Pseudomonas aeruginosa PAO1]
Identities = 112/112 (100%), Positives = 112/112 (100%)
Sequence:
ATGAAGCTAGTCACTGCCATCATCAAGCCGTTCAAGTTGGACGACGTCCGCGAGTCGTTGTCCGAGATCGGT
GTGCAGGGCATCACGGTGACCGAGGTCAAGGGTTTCGGCCGGCAAAAGGGCCACACCGAGCTGTATCGCGGC
GCCGAGTACGTCGTCGACTTCCTGCCCAAGGTGAAGATCGACGTGGCGATCGCCGACGACCAGCTGGACCGC
GTCATCGAGGCGATCACCAAGGCGGCCAATACCGGCAAGATCGGTGACGGCAAGATCTTCGTGGTGAACCTG
GAACAGGCCATCCGCATCCGTACCGGCGAAACCGATACCGACGCGATCTAA
Translation:
MKLVTAIIKPFKLDDVRESLSEIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIDVAIADDQLDR
VIEAITKAANTGKIGDGKIFVVNLEQAIRIRTGETDTDAI*
AnnotationID:5047GeneID:5042
AnnotatorUID: diggins
Modification Date/Time: 2005-03-16 09:09:08
Gene Name:glnK
Confidence Code:2
GeneProduct:Nitrogen regulatory protein PII
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0347,pfam00543
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL83553.1|AF414717_3 (AF414717) GlnK [Pseudomonas stutzeri]
          Length = 112

 Score =  218 bits (554), Expect = 7e-57
 Identities = 112/112 (100%), Positives = 112/112 (100%)

>gb|AAO53763.1| (AE016856) nitrogen regulatory protein P-II [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 112

 Score =  211 bits (536), Expect = 8e-55
 Identities = 109/112 (97%), Positives = 109/112 (97%)

>gb|AAN70799.1|AE016723_11 (AE016793) nitrogen regulatory protein P-II [Pseudomonas putida
           KT2440]
          Length = 112

 Score =  211 bits (536), Expect = 8e-55
 Identities = 109/112 (97%), Positives = 109/112 (97%)








 
Structural Features:
COG0347, GlnK, Nitrogen regulatory protein PII [Amino acid transport and metabolism]
CD-Length = 112 residues, 100.0% aligned
Score =  156 bits (397), Expect = 4e-40
Identities = 79/112 (70%), Positives = 94/112 (83%)

pfam00543, P-II, Nitrogen regulatory protein P-II. P-II modulates 
the activity of glutamine synthetase.
CD-Length = 106 residues, 100.0% aligned
Score =  124 bits (312), Expect = 3e-30
Identities = 59/106 (55%), Positives = 80/106 (75%)







   
Genomic Context:






   
Comment:


















    ReferenceID:10084




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:27225




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34805




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:5042_cdd.html


    		




 
URL:

















    ReferenceID:35716




  
Author/Investigator(s):
        Vermeiren H, Keijers V, Vanderleyden J.





  
Title:
        Isolation and sequence analysis of the GlnKamtB1amtB2 gene cluster, encoding a PII homologue and two putative ammonium transporters, from Pseudomonas stutzeri A15.




  
PubMed:
        12180140


MedLine:
        




    
Source:
DNA Seq. 2002 Feb;13(1):67-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12180140









           
Homologs By Global Alignment 

           

       



     
Gene ID:5042
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7448
                           PA5288_tr
                           translation of PA5288
                           112
                           99.99
                           99.99
                           0
                           554.0
     

     
     
192524
                           gb|AAG08673.1|AE004941_6
                           (AE004941) nitrogen regulatory protein P-II 2 [Pseudomonas aeruginosa PAO1]
                           112
                           99.99
                           99.99
                           0
                           554.0
     

     
     
192525
                           gb|AAL83553.1|AF414717_3
                           (AF414717) GlnK [Pseudomonas stutzeri]
                           112
                           99.99
                           99.99
                           0
                           554.0
     

     
     
192526
                           gb|AAO53763.1|
                           (AE016856) nitrogen regulatory protein P-II [Pseudomonas syringae pv. tomato str. DC3000]
                           112
                           97.32
                           97.32
                           0
                           536.0
     

     
     
192527
                           gb|AAN70799.1|AE016723_11
                           (AE016793) nitrogen regulatory protein P-II [Pseudomonas putida KT2440]
                           112
                           97.32
                           97.32
                           0
                           536.0
     

     






      

          
GC ORFID: 48044How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5288Glimmer Score: 

          
Start: 5489723Stop: 5489385

          
Length: 339


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5288 translation of PA5288Homolog Bit Score 218.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3959 (ORF: BLASTX 211.0), t_PA5288 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCTAGTCACTGCCATCATCAAGCCGTTCAAGTTGGACGACGTCCGCGAGTCGTTGTCCGAGATCGGT 
GTGCAGGGCATCACGGTGACCGAGGTCAAGGGTTTCGGCCGGCAAAAGGGCCACACCGAGCTGTATCGCGGC 
GCCGAGTACGTCGTCGACTTCCTGCCCAAGGTGAAGATCGACGTGGCGATCGCCGACGACCAGCTGGACCGC 
GTCATCGAGGCGATCACCAAGGCGGCCAATACCGGCAAGATCGGTGACGGCAAGATCTTCGTGGTGAACCTG 
GAACAGGCCATCCGCATCCGTACCGGCGAAACCGATACCGACGCGATCTAA