ViewGene
Locus Name:
GeneID:
Location:

GeneID:499
TrivialName: PA14_45000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45000
PAO1 Ortholog Locus:PA1502
Sequence Length:1776
Protein Length:591
Start: 4012138
Stop: 4013913
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04891.1|AE004579_3 (AE004579) glyoxylate carboligase [Pseudomonas aeruginosa PAO1]
Identities = 590/591 (99%), Positives = 591/591 (100%)
Sequence:
ATGGCCAGAATGAGAGCAATCGAAGCCGCCGTACTGGTGATGCGCCGCGAAGGCGTGGATACCGCGTTCGGC
GTCCCCGGTGCCGCGATCAACCCCATGTACGCCGCGCTGAAGAAGATCGGCGGCATCGACCATGTCCTGGCC
CGTCACGTCGAAGGCGCCTCGCACATGGCCGAGGGCTACACCCGTACCAAGGCCGGCAACATCGGCGTCTGC
ATCGGCACCTCCGGCCCTGCCGGCACCGACATGATCACCGGCCTCTACTCCGCCTCCGCCGACTCCATCCCG
ATTCTCTGCATCACCGGCCAGGCACCGCGCGCCCGCCTGCACAAGGAAGACTTCCAGGCGGTCGACATCTCC
AGCATCGCCGCGCCGGTGGCCAAGTGGGCGACCACCGTGCTGGAACCGGCCCAGGTCCCCTATGTATTCCAG
AAGGCCTTCCAGCTGATGCGCAGCGACCGGCCCGGCCCGGTACTGATCGACCTGCCGTTCGACGTACAGATG
GCCGAGATCGAGTTCGACATCGACGCCTACGAGCCCCTCCCGGTGAGCAAGCCGAAGGCCACCCGGGTCCAG
GCCGAGAAGGTCCTGGCCATGCTCGACGAAGCCGAGCGCCCGCTGCTGGTGGCCGGCGGCGGCATCTTCAAC
GCCGACGCCGCCGAGCGCTTCCGCGAATTCGCCGAGCTGACCGGCATCCCGGTGATCCCGACCCTGATGGGC
TGGGGCGTAATCCCCGACGACCACCCACAGATGGCCGGCATGGTCGGCCTGCAGACCTCGCACCGCTACGGC
AACGCCACCCTGCTGGAATCCGACCTGGTGCTCGGCATCGGCAACCGCTGGGCCAACCGCCACACCGGCAGC
GTCGAGGTCTATACCGAAGGTCGCCGCTTCATCCATGTCGACATCGAACCGACCCAGATCGGCCGGGTGTTC
ATGCCCGACCTGGGCATCGTCTCCGACGCCGGCGCCGCCCTCGACGCATTCCTCGAAGTGGCCCGCGAATGG
AAGGCCGCCGGCAAGCTGAAGTCGCGCGCCGCCTGGCTGGAAAGCTGCCAGCAGCGCAAACGCACGCTGCAG
CGCAAGACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTCTACGAAGAGATGAACGAGTTCTTCGGC
AAGGATGCCTGCTACGTCAGCACCATCGGCCTGTCGCAGATCGCCGGCGCACAGTTCCTCCACGTCTACCAA
CCGCGCCACTGGATCAACTGCGGCCAGGCCGGCCCGCTGGGCTGGACCATTCCCGCCGCGCTGGGGGTGGTC
AAGGCCGACCCGAGCCGCCAGGTGGTGGCGCTCTCCGGCGACTACGACTTCCAGTTCATGATCGAGGAACTG
GCGGTCGGCGCGCAGTTcAAGCTGCCCTATATCCACGTACTGGTGAACAACTCCTACCTGGGCCTGATCCGC
CAGGCGCAGCGCGGCTTCGACATGGACTATTGCGTGCAGCTGGCCTTCGACAACATCAACGCCCCCGAGCTG
GAAGGCTACGGCGTCGACCACGTCGCGGTGGTCGAGGGCCTGGGCTGCAAGGCGATCCGGGTGTTCGACCCG
AACGAGATCGGCGCCGCCCTGGCCAAGGCCCGCGAACTGATGCAGCAGCACCAGGTGCCGGTGGTGGTCGAG
GTGATCCTGGAGCGCGTCACCAACATCTCCATGGGTACCGAGATCAACGCCATCAACGAGTTCGAGGAACTG
GCCGAGAAAGGCGCCGACGCGCCGACCGCCATCTCGCTGCTCGACTGA
Translation:
MARMRAIEAAVLVMRREGVDTAFGVPGAAINPMYAALKKIGGIDHVLARHVEGASHMAEGYTRTKAGNIGVC
IGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLHKEDFQAVDISSIAAPVAKWATTVLEPAQVPYVFQ
KAFQLMRSDRPGPVLIDLPFDVQMAEIEFDIDAYEPLPVSKPKATRVQAEKVLAMLDEAERPLLVAGGGIFN
ADAAERFREFAELTGIPVIPTLMGWGVIPDDHPQMAGMVGLQTSHRYGNATLLESDLVLGIGNRWANRHTGS
VEVYTEGRRFIHVDIEPTQIGRVFMPDLGIVSDAGAALDAFLEVAREWKAAGKLKSRAAWLESCQQRKRTLQ
RKTHFDNVPVKPQRVYEEMNEFFGKDACYVSTIGLSQIAGAQFLHVYQPRHWINCGQAGPLGWTIPAALGVV
KADPSRQVVALSGDYDFQFMIEELAVGAQFKLPYIHVLVNNSYLGLIRQAQRGFDMDYCVQLAFDNINAPEL
EGYGVDHVAVVEGLGCKAIRVFDPNEIGAALAKARELMQQHQVPVVVEVILERVTNISMGTEINAINEFEEL
AEKGADAPTAISLLD*
AnnotationID:508GeneID:499
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 18:06:43
Gene Name:gcl
Confidence Code:2
GeneProduct:glyoxylate carboligase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3960,COG0028
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:4.1.1.47
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69877.1|AE016626_12 (AE016790) glyoxylate carboligase [Pseudomonas putida KT2440]
          Length = 591

 Score = 1036 bits (2680), Expect = 0.0
 Identities = 505/591 (85%), Positives = 546/591 (92%)

>emb|CAC49788.1| (AL603646) putative glyoxylate carboligase protein [Sinorhizobium
           meliloti]
          Length = 594

 Score =  941 bits (2432), Expect = 0.0
 Identities = 459/592 (77%), Positives = 515/592 (86%), Gaps = 2/592 (0%)

>gb|AAC73609.1| (AE000157) glyoxylate carboligase [Escherichia coli K12]
          Length = 593

 Score =  904 bits (2337), Expect = 0.0
 Identities = 439/585 (75%), Positives = 503/585 (85%)






 
Structural Features:
COG3960, COG3960, Glyoxylate carboligase
CD-Length = 592 residues,  99.7% aligned
Score = 1123 bits (2907), Expect = 0.0
Identities = 476/590 (80%), Positives = 520/590 (88%)

COG0028, IlvB, Thiamine pyrophosphate-requiring enzymes 
[acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, 
phosphonopyruvate decarboxylase] 
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 550 residues,  97.5% aligned
Score =  439 bits (1130), Expect = 4e-124
Identities = 205/562 (36%), Positives = 297/562 (52%), Gaps = 29/562 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1001




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18143




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37371




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:499_cdd.html


    		




 
URL:

















    ReferenceID:37944




  
Author/Investigator(s):
        Chang YY, Wang AY, Cronan JE Jr.





  
Title:
        Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family.




  
PubMed:
        8440684


MedLine:
        




    
Source:
J Biol Chem. 1993 Feb 25;268(6):3911-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8440684









           
Homologs By Global Alignment 

           

       



     
Gene ID:499
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
872
                           PA1502_tr
                           translation of PA1502
                           591
                           99.83
                           99.99
                           0
                           3050.0
     

     
     
36338
                           gb|AAG04891.1|AE004579_3
                           (AE004579) glyoxylate carboligase [Pseudomonas aeruginosa PAO1]
                           591
                           99.83
                           99.99
                           0
                           3050.0
     

     
     
36339
                           gb|AAN69877.1|AE016626_12
                           (AE016790) glyoxylate carboligase [Pseudomonas putida KT2440]
                           591
                           85.44
                           92.38
                           0
                           2680.0
     

     
     
36340
                           emb|CAC49788.1|
                           (AL603646) putative glyoxylate carboligase protein [Sinorhizobium meliloti]
                           594
                           77.60
                           87.03
                           3
                           2435.0
     

     
     
36341
                           dbj|BAB47877.1|
                           (AP002994) glyoxylate carboligase [Mesorhizobium loti]
                           594
                           76.09
                           86.19
                           4
                           2377.0
     

     






      

          
GC ORFID: 43501How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1502Glimmer Score: 

          
Start: 941771Stop: 939996

          
Length: 1776


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1502 translation of PA1502Homolog Bit Score 1179.0

          
Other Homologs:  t_PA1502 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCAGAATGAGAGCAATCGAAGCCGCCGTACTGGTGATGCGCCGCGAAGGCGTGGATACCGCGTTCGGC 
GTCCCCGGTGCCGCGATCAACCCCATGTACGCCGCGCTGAAGAAGATCGGCGGCATCGACCATGTCCTGGCC 
CGTCACGTCGAAGGCGCCTCGCACATGGCCGAGGGCTACACCCGTACCAAGGCCGGCAACATCGGCGTCTGC 
ATCGGCACCTCCGGCCCTGCCGGCACCGACATGATCACCGGCCTCTACTCCGCCTCCGCCGACTCCATCCCG 
ATTCTCTGCATCACCGGCCAGGCACCGCGCGCCCGCCTGCACAAGGAAGACTTCCAGGCGGTCGACATCTCC 
AGCATCGCCGCGCCGGTGGCCAAGTGGGCGACCACCGTGCTGGAACCGGCCCAGGTCCCCTATGTATTCCAG 
AAGGCCTTCCAGCTGATGCGCAGCGACCGGCCCGGCCCGGTACTGATCGACCTGCCGTTCGACGTACAGATG 
GCCGAGATCGAGTTCGACATCGACGCCTACGAGCCCCTCCCGGTGAGCAAGCCGAAGGCCACCCGGGTCCAG 
GCCGAGAAGGTCCTGGCCATGCTCGACGAAGCCGAGCGCCCGCTGCTGGTGGCCGGCGGCGGCATCTTCAAC 
GCCGACGCCGCCGAGCGCTTCCGCGAATTCGCCGAGCTGACCGGCATCCCGGTGATCCCGACCCTGATGGGC 
TGGGGCGTAATCCCCGACGACCACCCACAGATGGCCGGCATGGTCGGCCTGCAGACCTCGCACCGCTACGGC 
AACGCCACCCTGCTGGAATCCGACCTGGTGCTCGGCATCGGCAACCGCTGGGCCAACCGCCACACCGGCAGC 
GTCGAGGTCTATACCGAAGGTCGCCGCTTCATCCATGTCGACATCGAACCGACCCAGATCGGCCGGGTGTTC 
ATGCCCGACCTGGGCATCGTCTCCGACGCCGGCGCCGCCCTCGACGCATTCCTCGAAGTGGCCCGCGAATGG 
AAGGCCGCCGGCAAGCTGAAGTCGCGCGCCGCCTGGCTGGAAAGCTGCCAGCAGCGCAAACGCACGCTGCAG 
CGCAAGACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTCTACGAAGAGATGAACGAGTTCTTCGGC 
AAGGATGCCTGCTACGTCAGCACCATCGGCCTGTCGCAGATCGCCGGCGCACAGTTCCTCCACGTCTACCAA 
CCGCGCCACTGGATCAACTGCGGCCAGGCCGGCCCGCTGGGCTGGACCATTCCCGCCGCGCTGGGGGTGGTC 
AAGGCCGACCCGAGCCGCCAGGTGGTGGCGCTCTCCGGCGACTACGACTTCCAGTTCATGATCGAGGAACTG 
GCGGTCGGCGCGCAGTTcAAGCTGCCCTATATCCACGTACTGGTGAACAACTCCTACCTGGGCCTGATCCGC 
CAGGCGCAGCGCGGCTTCGACATGGACTATTGCGTGCAGCTGGCCTTCGACAACATCAACGCCCCCGAGCTG 
GAAGGCTACGGCGTCGACCACGTCGCGGTGGTCGAGGGCCTGGGCTGCAAGGCGATCCGGGTGTTCGACCCG 
AACGAGATCGGCGCCGCCCTGGCCAAGGCCCGCGAACTGATGCAGCAGCACCAGGTGCCGGTGGTGGTCGAG 
GTGATCCTGGAGCGCGTCACCAACATCTCCATGGGTACCGAGATCAACGCCATCAACGAGTTCGAGGAACTG 
GCCGAGAAAGGCGCCGACGCGCCGACCGCCATCTCGCTGCTCGACTGA