ViewGene
Locus Name:
GeneID:
Location:

GeneID:498
TrivialName: PA14_14780
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14780
PAO1 Ortholog Locus:PA3810
Sequence Length:1860
Protein Length:619
Start: 1256916
Stop: 1258775
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07197.1|AE004799_3 (AE004799) heat shock protein HscA [Pseudomonas aeruginosa PAO1]
Identities = 617/619 (99%), Positives = 619/619 (100%)
Sequence:
ATGGCCCTACTGCAGATCGCCGAGCCCGGTCAGAGTCCCAAGCCCCATGAGCGTCGCCTGGCGGTTGGGATC
GACCTGGGTACCACCAATTCGCTGGTCGCCGCGGTGCGTAGCGGCGTCGCCGAGCCCTTGCCGGATGCGCAG
GGGCGCCTGATCCTGCCTTCCGCGGTGCGTTATCACGCCGAGCGGGCGGAAGTGGGTGAGAGCGCCCGCGCG
GCTGCCGCGAAGGATCCCTTCAATACCATCATTTCGGTCAAGCGTCTGATGGGGCGCGGCCTGGAGGACGTC
AAGCAACTGGGCGAGCAATTGCCCTACCGCTTCCGCCAGGGCGAGTCGCACATGCCCTTCATCGAGACCGTA
CAGGGGCTGAAGAGTCCTGTGGAAGTCTCGGCGGACATCCTGCGTGAGCTGCGCCAGCGCGCCGAAACCACC
CTCGGTGGCGAGCTGGTGGGTGCGGTGATCACCGTGCCGGCCTATTTCGACGACGCCCAGCGCCAGGCTACC
AAGGATGCCGCGCGCCTGGCCGGACTGAACGTCCTGCGCCTGCTCAACGAGCCGACCGCCGCCGCCGTGGCC
TACGGCCTGGACAAGGGCGCGGAAGGCCTGGTGGCCATCTATGACCTGGGTGGTGGAACCTTCGACATTTCC
ATCCTGCGCCTGACCCGCGGTGTCTTCGAAGTTCTGGCCACCGGCGGCGATACGGCCCTGGGTGGCGACGAC
TTCGACCATGCCATCGCTGGCTGGGTGATCGAGCAGGCTGGCCTTTCTGCCGATCTGGACCCGGGCAGCCAG
CGGCAGTTGCTGCAAATCGCCTGTGCGGCCAAGGAGCGTCTTACCGATGAGGCGTCGGTCCGGGTCGCCTAT
GGCGACTGGAGCGGCGAGCTGAGCCGGGCGACGCTCGACGAGTTGATCGAGCCGTTCGTCGCTCGCAGCCTG
AAGTCCTGCCGCCGAGCGGTGCGCGACTCCGGTGTCGATCTGGAAGAAATCCGCTCTGTGGTGATGGTCGGT
GGCTCGACCCGCGTGCCCCGTGTGCGCACGGCGGTTGGCGAGTTGTTCGGCTGCGAGCCGCTGACCGATATC
GATCCGGACCAGGTGGTCGCCATCGGCGCGGCCATCCAGGCCGATGCCCTGGCCGGCAACAAGCGCGGCGAG
GAATTGCTGCTGCTGGACGTCATTCCGCTGTCGCTGGGCCTGGAAACCATGGGCGGGCTGATGGAGAAGGTG
ATTCCGCGTAATACCACCATCCCGGTGGCGCGCGCCCAGGAGTTCACCACCTACAAGGACGGCCAGACGGCC
ATGATGATCCACGTGCTGCAGGGCGAGCGCGAGCTGGTGAAGGATTGTCGTTCGCTGGCGCGCTTCGAGCTG
CGCGGCATTCCGCCGATGGTGGCGGGCGCGGCGAAAATCCGGGTGACCTTCCAGGTCGACGCCGACGGCTTG
CTCGGCGTGTCCGCACGCGAACTGAGTTCCGGCGTCGAGGCCAGCATCCAGGTCAAGCCGTCCTATGGGCTG
ACCGACGGCGAGATCGCGCGGATGCTCAAGGACTCCTTCGACTACGCCGGCGACGACAAGGCTGCCCGCGCC
CTGCGCGAACAGCAGGTCGAGGCGCAGCGGCTGCTGGAGGCCGTGCAATCGGCGCTGGACGTCGACGGCGAG
CGGCTCCTGGACGAAGAGGAGCGCCTGGCCATCGCGGCGCAGATGGACACCTTGCGTGAACTGGCCGGTGGT
AGCGACACCGCGGCCATCGAGAACCAGATCAAGCGCCTGTCCCAGGTGACCGACGCTTTCGCCGCACGGCGG
ATGGACGCGACCGTCAAGGCCGCGCTCTCCGGCCGCCGGCTCAACGAAATCGAGGAATAA
Translation:
MALLQIAEPGQSPKPHERRLAVGIDLGTTNSLVAAVRSGVAEPLPDAQGRLILPSAVRYHAERAEVGESARA
AAAKDPFNTIISVKRLMGRGLEDVKQLGEQLPYRFRQGESHMPFIETVQGLKSPVEVSADILRELRQRAETT
LGGELVGAVITVPAYFDDAQRQATKDAARLAGLNVLRLLNEPTAAAVAYGLDKGAEGLVAIYDLGGGTFDIS
ILRLTRGVFEVLATGGDTALGGDDFDHAIAGWVIEQAGLSADLDPGSQRQLLQIACAAKERLTDEASVRVAY
GDWSGELSRATLDELIEPFVARSLKSCRRAVRDSGVDLEEIRSVVMVGGSTRVPRVRTAVGELFGCEPLTDI
DPDQVVAIGAAIQADALAGNKRGEELLLLDVIPLSLGLETMGGLMEKVIPRNTTIPVARAQEFTTYKDGQTA
MMIHVLQGERELVKDCRSLARFELRGIPPMVAGAAKIRVTFQVDADGLLGVSARELSSGVEASIQVKPSYGL
TDGEIARMLKDSFDYAGDDKAARALREQQVEAQRLLEAVQSALDVDGERLLDEEERLAIAAQMDTLRELAGG
SDTAAIENQIKRLSQVTDAFAARRMDATVKAALSGRRLNEIEE*
AnnotationID:507GeneID:498
AnnotatorUID: danlee
Modification Date/Time: 2006-01-30 11:11:00
Gene Name:hscA
Confidence Code:1
GeneProduct:heat shock protein HscA
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00012,COG0443,KOG0100
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54948.1| (AE016860) chaperone protein HscA [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 620

 Score =  993 bits (2568), Expect = 0.0
 Identities = 510/620 (82%), Positives = 559/620 (90%), Gaps = 1/620 (0%)

>gb|AAC24478.1| (AF010139) heat-shock-cognate protein [Azotobacter vinelandii]
          Length = 619

 Score =  956 bits (2472), Expect = 0.0
 Identities = 501/620 (80%), Positives = 547/620 (88%), Gaps = 2/620 (0%)

>gb|AAN81501.1|AE016764_183 (AE016764) Chaperone protein hscA [Escherichia coli CFT073]
          Length = 634

 Score =  755 bits (1949), Expect = 0.0
 Identities = 407/617 (65%), Positives = 467/617 (75%), Gaps = 1/617 (0%)






 
Structural Features:
pfam00012, HSP70, Hsp70 protein.
CD-Length = 600 residues, 100.0% aligned
Score =  585 bits (1509), Expect = 1e-167
Identities = 255/600 (42%), Positives = 372/600 (62%), Gaps = 18/600 (3%)

COG0443, DnaK, Molecular chaperone 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 579 residues,  99.7% aligned
Score =  530 bits (1366), Expect = 4e-151
Identities = 270/601 (44%), Positives = 378/601 (62%), Gaps = 38/601 (6%)

KOG0100, Molecular chaperones GRP78/BiP/KAR2, HSP70 superfamily
CD-Length = 663 residues,  90.0% aligned
Score =  433 bits (1115), Expect = 5e-122
Identities = 237/602 (39%), Positives = 361/602 (59%), Gaps = 34/602 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:999




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18141




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31746




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:498_cdd.html


    		




 
URL:

















    ReferenceID:34295




  
Author/Investigator(s):
        Campos-Garcia J, G Ordonez L, Soberon-Chavez G.





  
Title:
        The Pseudomonas aeruginosa hscA gene encodes Hsc66, a DnaK homologue.




  
PubMed:
        10846221


MedLine:
        




    
Source:
Microbiology. 2000 Jun;146 ( Pt 6):1429-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10846221

















    ReferenceID:34296




  
Author/Investigator(s):
        Vickery LE, Silberg JJ, Ta DT.





  
Title:
        Hsc66 and Hsc20, a new heat shock cognate molecular chaperone system from Escherichia coli.




  
PubMed:
        9144776


MedLine:
        




    
Source:
Protein Sci. 1997 May;6(5):1047-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9144776

















    ReferenceID:34297




  
Author/Investigator(s):
        Takahashi Y, Nakamura M.





  
Title:
        Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli.




  
PubMed:
        10544286


MedLine:
        




    
Source:
J Biochem (Tokyo). 1999 Nov;126(5):917-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10544286









           
Homologs By Global Alignment 

           

       



     
Gene ID:498
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
870
                           PA3810_tr
                           translation of PA3810
                           619
                           99.67
                           99.99
                           0
                           3054.0
     

     
     
36083
                           gb|AAG07197.1|AE004799_3
                           (AE004799) heat shock protein HscA [Pseudomonas aeruginosa PAO1]
                           619
                           99.67
                           99.99
                           0
                           3054.0
     

     
     
36084
                           gb|AAC79497.2|
                           (AF096864) heat shock protein 66-KDa [Pseudomonas aeruginosa]
                           619
                           88.69
                           93.86
                           0
                           2729.0
     

     
     
36086
                           gb|AAN66471.1|AE016276_6
                           (AE016777) heat shock protein HscA [Pseudomonas putida KT2440]
                           620
                           82.58
                           89.51
                           1
                           2538.0
     

     
     
36085
                           gb|AAO54948.1|
                           (AE016860) chaperone protein HscA [Pseudomonas syringae pv. tomato str. DC3000]
                           620
                           82.25
                           90.16
                           1
                           2570.0
     

     






      

          
GC ORFID: 43500How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3810Glimmer Score: 

          
Start: 3697022Stop: 3695163

          
Length: 1860


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3810 translation of PA3810Homolog Bit Score 1181.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4521 (ORF: BLASTX 999.0), t_PA4761 (ORF: BLASTX 425.0), t_PA3810 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCCTACTGCAGATCGCCGAGCCCGGTCAGAGTCCCAAGCCCCATGAGCGTCGCCTGGCGGTTGGGATC 
GACCTGGGTACCACCAATTCGCTGGTCGCCGCGGTGCGTAGCGGCGTCGCCGAGCCCTTGCCGGATGCGCAG 
GGGCGCCTGATCCTGCCTTCCGCGGTGCGTTATCACGCCGAGCGGGCGGAAGTGGGTGAGAGCGCCCGCGCG 
GCTGCCGCGAAGGATCCCTTCAATACCATCATTTCGGTCAAGCGTCTGATGGGGCGCGGCCTGGAGGACGTC 
AAGCAACTGGGCGAGCAATTGCCCTACCGCTTCCGCCAGGGCGAGTCGCACATGCCCTTCATCGAGACCGTA 
CAGGGGCTGAAGAGTCCTGTGGAAGTCTCGGCGGACATCCTGCGTGAGCTGCGCCAGCGCGCCGAAACCACC 
CTCGGTGGCGAGCTGGTGGGTGCGGTGATCACCGTGCCGGCCTATTTCGACGACGCCCAGCGCCAGGCTACC 
AAGGATGCCGCGCGCCTGGCCGGACTGAACGTCCTGCGCCTGCTCAACGAGCCGACCGCCGCCGCCGTGGCC 
TACGGCCTGGACAAGGGCGCGGAAGGCCTGGTGGCCATCTATGACCTGGGTGGTGGAACCTTCGACATTTCC 
ATCCTGCGCCTGACCCGCGGTGTCTTCGAAGTTCTGGCCACCGGCGGCGATACGGCCCTGGGTGGCGACGAC 
TTCGACCATGCCATCGCTGGCTGGGTGATCGAGCAGGCTGGCCTTTCTGCCGATCTGGACCCGGGCAGCCAG 
CGGCAGTTGCTGCAAATCGCCTGTGCGGCCAAGGAGCGTCTTACCGATGAGGCGTCGGTCCGGGTCGCCTAT 
GGCGACTGGAGCGGCGAGCTGAGCCGGGCGACGCTCGACGAGTTGATCGAGCCGTTCGTCGCTCGCAGCCTG 
AAGTCCTGCCGCCGAGCGGTGCGCGACTCCGGTGTCGATCTGGAAGAAATCCGCTCTGTGGTGATGGTCGGT 
GGCTCGACCCGCGTGCCCCGTGTGCGCACGGCGGTTGGCGAGTTGTTCGGCTGCGAGCCGCTGACCGATATC 
GATCCGGACCAGGTGGTCGCCATCGGCGCGGCCATCCAGGCCGATGCCCTGGCCGGCAACAAGCGCGGCGAG 
GAATTGCTGCTGCTGGACGTCATTCCGCTGTCGCTGGGCCTGGAAACCATGGGCGGGCTGATGGAGAAGGTG 
ATTCCGCGTAATACCACCATCCCGGTGGCGCGCGCCCAGGAGTTCACCACCTACAAGGACGGCCAGACGGCC 
ATGATGATCCACGTGCTGCAGGGCGAGCGCGAGCTGGTGAAGGATTGTCGTTCGCTGGCGCGCTTCGAGCTG 
CGCGGCATTCCGCCGATGGTGGCGGGCGCGGCGAAAATCCGGGTGACCTTCCAGGTCGACGCCGACGGCTTG 
CTCGGCGTGTCCGCACGCGAACTGAGTTCCGGCGTCGAGGCCAGCATCCAGGTCAAGCCGTCCTATGGGCTG 
ACCGACGGCGAGATCGCGCGGATGCTCAAGGACTCCTTCGACTACGCCGGCGACGACAAGGCTGCCCGCGCC 
CTGCGCGAACAGCAGGTCGAGGCGCAGCGGCTGCTGGAGGCCGTGCAATCGGCGCTGGACGTCGACGGCGAG 
CGGCTCCTGGACGAAGAGGAGCGCCTGGCCATCGCGGCGCAGATGGACACCTTGCGTGAACTGGCCGGTGGT 
AGCGACACCGCGGCCATCGAGAACCAGATCAAGCGCCTGTCCCAGGTGACCGACGCTTTCGCCGCACGGCGG 
ATGGACGCGACCGTCAAGGCCGCGCTCTCCGGCCGCCGGCTCAACGAAATCGAGGAATAA